Restriction map for
pF1KB5951
(Circular) (Six-base) MAPSORT of: pF1KB5951.seq Check: 9088 from: 1 to: 4643
pF1KB5951 4643 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 1439 1681 1439
Size: 242 4401
Acc65I G'GTAC_C
Cuts at: 1659 1659
Size: 4643
AclI AA'CG_TT
Cuts at: 4483 4483
Size: 4643
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 305 1195 1528 2468 2900 3647 3915 257
Size: 48 890 333 940 432 747 268 985
Fragments arranged by size:
985 940 890 747 432 333 268 48
AflIII A'CryG_T
Cuts at: 1995 3099 4076 1995
Size: 1104 977 2562
Fragments arranged by size:
2562 1104 977
AgeI A'CCGG_T
Cuts at: 3031 3031
Size: 4643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 614 695 614
Size: 81 4562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 299 331 845 877 914 946 299
Size: 32 514 32 37 32 3996
Fragments arranged by size:
3996 514 37 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 658 708 1227 1948 3515 658
Size: 50 519 721 1567 1786
Fragments arranged by size:
1786 1567 721 519 50
ApaI G_GGCC'C
Cuts at: 628 950 1187 628
Size: 322 237 4084
Fragments arranged by size:
4084 322 237
ApaLI G'TGCA_C
Cuts at: 3413 3413
Size: 4643
ApoI r'AATT_y
Cuts at: 1444 1647 3996 4252 1444
Size: 203 2349 256 1835
Fragments arranged by size:
2349 1835 256 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4643
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
AvaI C'yCGr_G
Cuts at: 933 995 1663 933
Size: 62 668 3913
Fragments arranged by size:
3913 668 62
BamHI G'GATC_C
Cuts at: 1668 1668
Size: 4643
BanI G'GyrC_C
Cuts at: 1659 2073 2321 2356 1659
Size: 414 248 35 3946
Fragments arranged by size:
3946 414 248 35
BanII G_rGCy'C
Cuts at: 628 950 1187 1657 2687 628
Size: 322 237 470 1030 2584
Fragments arranged by size:
2584 1030 470 322 237
BbeI G_GCGC'C
Cuts at: 2077 2325 2077
Size: 248 4395
BbsI GAAGACnn'nnnn_
Cuts at: 1574 1574
Size: 4643
BbvCI CC'TCA_GC
Cuts at: 800 1531 800
Size: 731 3912
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 976 1010 1612 1646 976
Size: 34 602 34 3973
Fragments arranged by size:
3973 602 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 752 2541 3302 4643 752
Size: 1789 761 1341 752
Fragments arranged by size:
1789 1341 761 752
BclI T'GATC_A
Cuts at: 123 2163 123
Size: 2040 2603
BglII A'GATC_T
Cuts at: 219 2158 4195 219
Size: 1939 2037 667
Fragments arranged by size:
2037 1939 667
BlpI GC'TnA_GC
Cuts at: 1372 1755 1372
Size: 383 4260
Bme1580I G_kGCm'C
Cuts at: 628 950 1187 2268 2361 3417 628
Size: 322 237 1081 93 1056 1854
Fragments arranged by size:
1854 1081 1056 322 237 93
BmrI ACTGGGnnnn_n'
Cuts at: 604 824 1855 1936 2271 4415 604
Size: 220 1031 81 335 2144 832
Fragments arranged by size:
2144 1031 832 335 220 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1397 3010 1397
Size: 1613 3030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 836 1047 1445 836
Size: 211 398 4034
Fragments arranged by size:
4034 398 211
Bpu10I CC'TnA_GC
Cuts at: 800 1531 800
Size: 731 3912
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1819 2698 3190 3488 1819
Size: 879 492 298 2974
Fragments arranged by size:
2974 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4347 4347
Size: 4643
BsaAI yAC'GTr
Cuts at: 1996 1996
Size: 4643
BsaBI GATnn'nnATC
Cuts at: 6 2157 4194 6
Size: 2151 2037 455
Fragments arranged by size:
2151 2037 455
BsaHI Gr'CG_yC
Cuts at: 2074 2322 3024 4520 2074
Size: 248 702 1496 2197
Fragments arranged by size:
2197 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1308 1832 2353 3031 3305 3452 3863 1308
Size: 524 521 678 274 147 411 2088
Fragments arranged by size:
2088 678 524 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 376 406 376
Size: 30 4613
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 384 788 1552 384
Size: 404 764 3475
Fragments arranged by size:
3475 764 404
BseYI C'CCAG_C
Cuts at: 951 1248 1328 2068 2826 3403 3899 951
Size: 297 80 740 758 577 496 1695
Fragments arranged by size:
1695 758 740 577 496 297 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 4643
BsiEI CG_ry'CG
Cuts at: 104 2185 2231 3439 104
Size: 2081 46 1208 1308
Fragments arranged by size:
2081 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1657 2435 2625 3417 1657
Size: 778 190 792 2883
Fragments arranged by size:
2883 792 778 190
BsmI GAATG_Cn'
Cuts at: 175 175
Size: 4643
Bsp1286I G_dGCh'C
Cuts at: 628 950 1187 1657 2268 2361 2435 2625 2687
Size: 322 237 470 611 93 74 190 62
Cuts at: 2687 3417 628
Size: 730 1854
Fragments arranged by size:
1854 730 611 470 322 237 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3863 3863
Size: 4643
BspHI T'CATG_A
Cuts at: 3819 3819
Size: 4643
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1693 1907 2209 2590 1693
Size: 214 302 381 3746
Fragments arranged by size:
3746 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1379 2935 2989 4473 4638 50
Size: 1329 1556 54 1484 165 55
Fragments arranged by size:
1556 1484 1329 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1265 2555 1265
Size: 1290 3353
BsrFI r'CCGG_y
Cuts at: 1346 2641 2822 3031 1346
Size: 1295 181 209 2958
Fragments arranged by size:
2958 1295 209 181
BssSI C'ACGA_G
Cuts at: 2914 3272 2914
Size: 358 4285
BstAPI GCAn_nnn'nTGC
Cuts at: 2020 2020
Size: 4643
BstBI TT'CG_AA
Cuts at: 3004 3004
Size: 4643
BstEII G'GTnAC_C
Cuts at: 1230 1230
Size: 4643
BstYI r'GATC_y
Cuts at: 1 219 252 1095 1668 2135 2158 2493 2739
Size: 218 33 843 573 467 23 335 246
Cuts at: 2739 3751 3837 3849 4195 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 843 573 467 449 346 335 246
218 86 33 23 12
Bsu36I CC'TnA_GG
Cuts at: 566 585 884 566
Size: 19 299 4325
Fragments arranged by size:
4325 299 19
BtgI C'CryG_G
Cuts at: 107 1475 107
Size: 1368 3275
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2593 2774 2937 2593
Size: 181 163 4299
Fragments arranged by size:
4299 181 163
BtsI GCAGTG_nn'
Cuts at: 1886 1886
Size: 4643
DraI TTT'AAA
Cuts at: 1642 1642
Size: 4643
DrdI GACnn_nn'nnGTC
Cuts at: 2349 3207 2349
Size: 858 3785
EaeI y'GGCC_r
Cuts at: 1348 2228 2402 2793 2820 4165 1348
Size: 880 174 391 27 1345 1826
Fragments arranged by size:
1826 1345 880 391 174 27
EagI C'GGCC_G
Cuts at: 2228 2228
Size: 4643
EarI CTCTTCn'nnn_
Cuts at: 233 1307 1474 2666 2876 233
Size: 1074 167 1192 210 2000
Fragments arranged by size:
2000 1192 1074 210 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2851 3161 3307 4453 2851
Size: 310 146 1146 3041
Fragments arranged by size:
3041 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1655 1655
Size: 4643
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 257 305 836 1047 1195 1445 1528 2468 2900
Size: 48 531 211 148 250 83 940 432
Cuts at: 2900 3647 3915 257
Size: 747 268 985
Fragments arranged by size:
985 940 747 531 432 268 250 211
148 83 48
EcoNI CCTnn'n_nnAGG
Cuts at: 477 803 1534 477
Size: 326 731 3586
Fragments arranged by size:
3586 731 326
EcoO109I rG'GnC_Cy
Cuts at: 625 930 946 947 1183 1184 1782 625
Size: 305 16 1 236 1 598 3486
Fragments arranged by size:
3486 598 305 236 16 1 1
EcoRI G'AATT_C
Cuts at: 1647 1647
Size: 4643
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4643
FspI TGC'GCA
Cuts at: 2424 2424
Size: 4643
HaeII r_GCGC'y
Cuts at: 1329 2077 2325 3347 4326 1329
Size: 748 248 1022 979 1646
Fragments arranged by size:
1646 1022 979 748 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 313 345 391 423 2485 2515 2517 2547 313
Size: 32 46 32 2062 30 2 30 2409
Fragments arranged by size:
2409 2062 46 32 32 30 30 2
HincII GTy'rAC
Cuts at: 262 1682 262
Size: 1420 3223
KasI G'GCGC_C
Cuts at: 2073 2321 2073
Size: 248 4395
KpnI G_GTAC'C
Cuts at: 1663 1663
Size: 4643
MluI A'CGCG_T
Cuts at: 4076 4076
Size: 4643
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 385 3314 3498 385
Size: 2929 184 1530
Fragments arranged by size:
2929 1530 184
MscI TGG'CCA
Cuts at: 2404 2404
Size: 4643
MslI CAynn'nnrTG
Cuts at: 2632 2759 2632
Size: 127 4516
MspA1I CmG'CkG
Cuts at: 604 1215 1554 1601 1754 2068 2428 3441 3686
Size: 611 339 47 153 314 360 1013 245
Cuts at: 3686 604
Size: 1561
Fragments arranged by size:
1561 1013 611 360 339 314 245 153
47
NaeI GCC'GGC
Cuts at: 2824 2824
Size: 4643
NarI GG'CG_CC
Cuts at: 2074 2322 2074
Size: 248 4395
NcoI C'CATG_G
Cuts at: 107 1475 107
Size: 1368 3275
NdeI CA'TA_TG
Cuts at: 1086 1086
Size: 4643
NgoMIV G'CCGG_C
Cuts at: 2822 2822
Size: 4643
NspI r_CATG'y
Cuts at: 595 942 1696 2196 3103 595
Size: 347 754 500 907 2135
Fragments arranged by size:
2135 907 754 500 347
PasI CC'CwG_GG
Cuts at: 148 148
Size: 4643
PciI A'CATG_T
Cuts at: 3099 3099
Size: 4643
PflMI CCAn_nnn'nTGG
Cuts at: 658 658
Size: 4643
PfoI T'CCnGG_A
Cuts at: 813 1484 1586 813
Size: 671 102 3870
Fragments arranged by size:
3870 671 102
PmeI GTTT'AAAC
Cuts at: 1642 1642
Size: 4643
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1188 1220 3782 3814 1188
Size: 32 2562 32 2017
Fragments arranged by size:
2562 2017 32 32
PpuMI rG'GwC_Cy
Cuts at: 930 930
Size: 4643
PshAI GACnn'nnGTC
Cuts at: 771 771
Size: 4643
PspOMI G'GGCC_C
Cuts at: 624 946 1183 624
Size: 322 237 4084
Fragments arranged by size:
4084 322 237
PstI C_TGCA'G
Cuts at: 603 713 870 1606 1690 2375 603
Size: 110 157 736 84 685 2871
Fragments arranged by size:
2871 736 685 157 110 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4643
PvuII CAG'CTG
Cuts at: 604 1215 2068 2428 604
Size: 611 853 360 2819
Fragments arranged by size:
2819 853 611 360
RsrII CG'GwC_CG
Cuts at: 2838 2838
Size: 4643
SacI G_AGCT'C
Cuts at: 1657 1657
Size: 4643
SalI G'TCGA_C
Cuts at: 1680 1680
Size: 4643
SanDI GG'GwC_CC
Cuts at: 930 930
Size: 4643
SapI GCTCTTCn'nnn_
Cuts at: 233 2666 2876 233
Size: 2433 210 2000
Fragments arranged by size:
2433 2000 210
SbfI CC_TGCA'GG
Cuts at: 1690 1690
Size: 4643
ScaI AGT'ACT
Cuts at: 4160 4160
Size: 4643
SexAI A'CCwGG_T
Cuts at: 338 338
Size: 4643
SfcI C'TryA_G
Cuts at: 33 599 709 866 1602 1686 2371 3364 3555
Size: 566 110 157 736 84 685 993 191
Cuts at: 3555 33
Size: 1121
Fragments arranged by size:
1121 993 736 685 566 191 157 110
84
SfoI GGC'GCC
Cuts at: 2075 2323 2075
Size: 248 4395
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4643
SmaI CCC'GGG
Cuts at: 935 1665 935
Size: 730 3913
SmlI C'TyrA_G
Cuts at: 1798 2713 3205 3467 1798
Size: 915 492 262 2974
Fragments arranged by size:
2974 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4643
SphI G_CATG'C
Cuts at: 595 942 1696 2196 595
Size: 347 754 500 3042
Fragments arranged by size:
3042 754 500 347
StyI C'CwwG_G
Cuts at: 107 1475 1777 2131 107
Size: 1368 302 354 2619
Fragments arranged by size:
2619 1368 354 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 549 553 2226 2359 2818 3027 549
Size: 4 1673 133 459 209 2165
Fragments arranged by size:
2165 1673 459 209 133 4
TatI w'GTAC_w
Cuts at: 4158 4158
Size: 4643
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 376 408 947 979 4160 4192 376
Size: 32 539 32 3181 32 827
Fragments arranged by size:
3181 827 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2440 2440
Size: 4643
XbaI T'CTAG_A
Cuts at: 1674 1674
Size: 4643
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2138 2138
Size: 4643
XmaI C'CCGG_G
Cuts at: 933 1663 933
Size: 730 3913
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PmlI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI