Restriction map for
pF1KB5952
(Circular) (Six-base) MAPSORT of: pF1KB5952.seq Check: 8052 from: 1 to: 4754
pF1KB5952 4754 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AatII G_ACGT'C
Cuts at: 310 310
Size: 4754
AccI GT'mk_AC
Cuts at: 869 1792 869
Size: 923 3831
Acc65I G'GTAC_C
Cuts at: 120 1770 120
Size: 1650 3104
AclI AA'CG_TT
Cuts at: 4594 4594
Size: 4754
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1171 1620 2579 3011 3758 4026 1171
Size: 449 959 432 747 268 1899
Fragments arranged by size:
1899 959 747 449 432 268
AflIII A'CryG_T
Cuts at: 1362 2106 3210 4187 1362
Size: 744 1104 977 1929
Fragments arranged by size:
1929 1104 977 744
AgeI A'CCGG_T
Cuts at: 3142 3142
Size: 4754
AleI CACnn'nnGTG
Cuts at: 562 562
Size: 4754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1705 1705
Size: 4754
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1243 1275 1243
Size: 32 4722
AlwNI CAG_nnn'CTG
Cuts at: 146 708 894 2059 3626 146
Size: 562 186 1165 1567 1274
Fragments arranged by size:
1567 1274 1165 562 186
ApaLI G'TGCA_C
Cuts at: 3524 3524
Size: 4754
ApoI r'AATT_y
Cuts at: 1758 4107 4363 1758
Size: 2349 256 2149
Fragments arranged by size:
2349 2149 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4754
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
AvaI C'yCGr_G
Cuts at: 974 1022 1127 1167 1243 1399 1774 974
Size: 48 105 40 76 156 375 3954
Fragments arranged by size:
3954 375 156 105 76 48 40
AvrII C'CTAG_G
Cuts at: 999 1050 999
Size: 51 4703
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 104 137 104
Size: 33 4721
BamHI G'GATC_C
Cuts at: 386 1448 1779 386
Size: 1062 331 3361
Fragments arranged by size:
3361 1062 331
BanI G'GyrC_C
Cuts at: 120 474 970 1437 1770 2184 2432 2467 120
Size: 354 496 467 333 414 248 35 2407
Fragments arranged by size:
2407 496 467 414 354 333 248 35
BanII G_rGCy'C
Cuts at: 221 666 1079 1128 1558 1768 2798 221
Size: 445 413 49 430 210 1030 2177
Fragments arranged by size:
2177 1030 445 430 413 210 49
BbeI G_GCGC'C
Cuts at: 2188 2436 2188
Size: 248 4506
BbsI GAAGACnn'nnnn_
Cuts at: 1566 1626 1566
Size: 60 4694
BbvCI CC'TCA_GC
Cuts at: 729 1069 1745 729
Size: 340 676 3738
Fragments arranged by size:
3738 676 340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1307 1341 1307
Size: 34 4720
BciVI GTATCCnnnnn_n'
Cuts at: 2652 3413 4754 2652
Size: 761 1341 2652
Fragments arranged by size:
2652 1341 761
BclI T'GATC_A
Cuts at: 2274 2274
Size: 4754
BglI GCCn_nnn'nGGC
Cuts at: 489 705 859 489
Size: 216 154 4384
Fragments arranged by size:
4384 216 154
BglII A'GATC_T
Cuts at: 2269 4306 2269
Size: 2037 2717
BlpI GC'TnA_GC
Cuts at: 589 1866 589
Size: 1277 3477
Bme1580I G_kGCm'C
Cuts at: 975 2379 2472 3528 975
Size: 1404 93 1056 2201
Fragments arranged by size:
2201 1404 1056 93
BmgBI CAC'GTC
Cuts at: 1365 1700 1365
Size: 335 4419
BmrI ACTGGGnnnn_n'
Cuts at: 1966 2047 2382 4526 1966
Size: 81 335 2144 2194
Fragments arranged by size:
2194 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 4754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1410 263
Size: 1147 3607
Bpu10I CC'TnA_GC
Cuts at: 729 1069 1745 729
Size: 340 676 3738
Fragments arranged by size:
3738 676 340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1930 2809 3301 3599 1930
Size: 879 492 298 3085
Fragments arranged by size:
3085 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4458 4458
Size: 4754
BsaAI yAC'GTr
Cuts at: 2107 2107
Size: 4754
BsaBI GATnn'nnATC
Cuts at: 6 2268 4305 6
Size: 2262 2037 455
Fragments arranged by size:
2262 2037 455
BsaHI Gr'CG_yC
Cuts at: 307 2185 2433 3135 4631 307
Size: 1878 248 702 1496 430
Fragments arranged by size:
1878 1496 702 430 248
BsaWI w'CCGG_w
Cuts at: 1943 2464 3142 3416 3563 3974 1943
Size: 521 678 274 147 411 2723
Fragments arranged by size:
2723 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 523 553 1644 1674 523
Size: 30 1091 30 3603
Fragments arranged by size:
3603 1091 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 420 595 598 625 1690 420
Size: 175 3 27 1065 3484
Fragments arranged by size:
3484 1065 175 27 3
BseYI C'CCAG_C
Cuts at: 280 344 575 708 879 1120 1331 1549 2179
Size: 64 231 133 171 241 211 218 630
Cuts at: 2179 2937 3514 4010 280
Size: 758 577 496 1024
Fragments arranged by size:
1024 758 630 577 496 241 231 218
211 171 133 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 4754
BsiEI CG_ry'CG
Cuts at: 104 1160 2296 2342 3550 104
Size: 1056 1136 46 1208 1308
Fragments arranged by size:
1308 1208 1136 1056 46
BsiHKAI G_wGCw'C
Cuts at: 236 1558 1646 1684 1768 2546 2736 3528 236
Size: 1322 88 38 84 778 190 792 1462
Fragments arranged by size:
1462 1322 792 778 190 88 84 38
BsmI GAATG_Cn'
Cuts at: 903 903
Size: 4754
BsmBI CGTCTCn'nnnn_
Cuts at: 298 644 1005 298
Size: 346 361 4047
Fragments arranged by size:
4047 361 346
Bsp1286I G_dGCh'C
Cuts at: 221 236 666 975 1079 1128 1558 1646 1684
Size: 15 430 309 104 49 430 88 38
Cuts at: 1684 1768 2379 2472 2546 2736 2798 3528 221
Size: 84 611 93 74 190 62 730 1447
Fragments arranged by size:
1447 730 611 430 430 309 190 104
93 88 84 74 62 49 38 15
BspEI T'CCGG_A
Cuts at: 3974 3974
Size: 4754
BspHI T'CATG_A
Cuts at: 908 3930 908
Size: 3022 1732
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1804 2018 2320 2701 1804
Size: 214 302 381 3857
Fragments arranged by size:
3857 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1655 3046 3100 4584 4749 50
Size: 1605 1391 54 1484 165 55
Fragments arranged by size:
1605 1484 1391 165 55 54
BsrDI GCAATG_nn'
Cuts at: 414 1727 2666 414
Size: 1313 939 2502
Fragments arranged by size:
2502 1313 939
BsrFI r'CCGG_y
Cuts at: 495 593 819 1440 2752 2933 3142 495
Size: 98 226 621 1312 181 209 2107
Fragments arranged by size:
2107 1312 621 226 209 181 98
BsrGI T'GTAC_A
Cuts at: 446 446
Size: 4754
BssHII G'CGCG_C
Cuts at: 952 954 952
Size: 2 4752
BssSI C'ACGA_G
Cuts at: 781 1508 3025 3383 781
Size: 727 1517 358 2152
Fragments arranged by size:
2152 1517 727 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2131 2131
Size: 4754
BstBI TT'CG_AA
Cuts at: 3115 3115
Size: 4754
BstXI CCAn_nnnn'nTGG
Cuts at: 339 639 339
Size: 300 4454
BstYI r'GATC_y
Cuts at: 1 386 1448 1779 2246 2269 2604 2850 3862
Size: 385 1062 331 467 23 335 246 1012
Cuts at: 3862 3948 3960 4306 1
Size: 86 12 346 449
Fragments arranged by size:
1062 1012 467 449 385 346 335 331
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 116 657 915 947 1160 107
Size: 9 541 258 32 213 3701
Fragments arranged by size:
3701 541 258 213 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2704 2885 3048 2704
Size: 181 163 4410
Fragments arranged by size:
4410 181 163
BtsI GCAGTG_nn'
Cuts at: 611 626 1239 1378 1997 611
Size: 15 613 139 619 3368
Fragments arranged by size:
3368 619 613 139 15
DraI TTT'AAA
Cuts at: 1753 1753
Size: 4754
DrdI GACnn_nn'nnGTC
Cuts at: 2460 3318 2460
Size: 858 3896
EaeI y'GGCC_r
Cuts at: 110 329 641 817 932 1157 2339 2513 2904
Size: 219 312 176 115 225 1182 174 391
Cuts at: 2904 2931 4276 110
Size: 27 1345 588
Fragments arranged by size:
1345 1182 588 391 312 225 219 176
174 115 27
EagI C'GGCC_G
Cuts at: 1157 2339 1157
Size: 1182 3572
EarI CTCTTCn'nnn_
Cuts at: 2777 2987 2777
Size: 210 4544
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2962 3272 3418 4564 2962
Size: 310 146 1146 3152
Fragments arranged by size:
3152 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1556 1766 1556
Size: 210 4544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1171 1410 1620 2579 3011 3758 4026 263
Size: 908 239 210 959 432 747 268 991
Fragments arranged by size:
991 959 908 747 432 268 239 210
EcoNI CCTnn'n_nnAGG
Cuts at: 316 316
Size: 4754
EcoO109I rG'GnC_Cy
Cuts at: 143 248 1054 1893 143
Size: 105 806 839 3004
Fragments arranged by size:
3004 839 806 105
EcoRI G'AATT_C
Cuts at: 1758 1758
Size: 4754
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2773 2773
Size: 4754
FspI TGC'GCA
Cuts at: 2535 2535
Size: 4754
HaeII r_GCGC'y
Cuts at: 880 2188 2436 3458 4437 880
Size: 1308 248 1022 979 1197
Fragments arranged by size:
1308 1197 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 523 555 1121 1153 1671 1703 2596 2626 2628
Size: 32 566 32 518 32 893 30 2
Cuts at: 2628 2658 523
Size: 30 2619
Fragments arranged by size:
2619 893 566 518 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1464 1793 1464
Size: 329 4425
KasI G'GCGC_C
Cuts at: 2184 2432 2184
Size: 248 4506
KpnI G_GTAC'C
Cuts at: 124 1774 124
Size: 1650 3104
MluI A'CGCG_T
Cuts at: 4187 4187
Size: 4754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 364 3425 3609 364
Size: 3061 184 1509
Fragments arranged by size:
3061 1509 184
MscI TGG'CCA
Cuts at: 112 331 643 2515 112
Size: 219 312 1872 2351
Fragments arranged by size:
2351 1872 312 219
MslI CAynn'nnrTG
Cuts at: 442 562 1702 2743 2870 442
Size: 120 1140 1041 127 2326
Fragments arranged by size:
2326 1140 1041 127 120
MspA1I CmG'CkG
Cuts at: 619 1162 1287 1865 2179 2539 3552 3797 619
Size: 543 125 578 314 360 1013 245 1576
Fragments arranged by size:
1576 1013 578 543 360 314 245 125
NaeI GCC'GGC
Cuts at: 595 2935 595
Size: 2340 2414
NarI GG'CG_CC
Cuts at: 2185 2433 2185
Size: 248 4506
NcoI C'CATG_G
Cuts at: 107 116 107
Size: 9 4745
NgoMIV G'CCGG_C
Cuts at: 593 2933 593
Size: 2340 2414
NspI r_CATG'y
Cuts at: 696 1328 1807 2307 3214 696
Size: 632 479 500 907 2236
Fragments arranged by size:
2236 907 632 500 479
PasI CC'CwG_GG
Cuts at: 140 702 1596 140
Size: 562 894 3298
Fragments arranged by size:
3298 894 562
PciI A'CATG_T
Cuts at: 3210 3210
Size: 4754
PflMI CCAn_nnn'nTGG
Cuts at: 708 708
Size: 4754
PfoI T'CCnGG_A
Cuts at: 455 455
Size: 4754
PmeI GTTT'AAAC
Cuts at: 1753 1753
Size: 4754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 3893
Size: 32 4722
PpuMI rG'GwC_Cy
Cuts at: 248 1054 248
Size: 806 3948
PspXI vC'TCGA_Gb
Cuts at: 1127 1127
Size: 4754
PstI C_TGCA'G
Cuts at: 268 1234 1373 1801 2486 268
Size: 966 139 428 685 2536
Fragments arranged by size:
2536 966 685 428 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4754
PvuII CAG'CTG
Cuts at: 1287 2179 2539 1287
Size: 892 360 3502
Fragments arranged by size:
3502 892 360
RsrII CG'GwC_CG
Cuts at: 2949 2949
Size: 4754
SacI G_AGCT'C
Cuts at: 1558 1768 1558
Size: 210 4544
SacII CC_GC'GG
Cuts at: 1163 1163
Size: 4754
SalI G'TCGA_C
Cuts at: 1791 1791
Size: 4754
SanDI GG'GwC_CC
Cuts at: 248 248
Size: 4754
SapI GCTCTTCn'nnn_
Cuts at: 2777 2987 2777
Size: 210 4544
SbfI CC_TGCA'GG
Cuts at: 1801 1801
Size: 4754
ScaI AGT'ACT
Cuts at: 270 4271 270
Size: 4001 753
SfcI C'TryA_G
Cuts at: 33 264 1230 1369 1797 2482 3475 3666 33
Size: 231 966 139 428 685 993 191 1121
Fragments arranged by size:
1121 993 966 685 428 231 191 139
SfoI GGC'GCC
Cuts at: 2186 2434 2186
Size: 248 4506
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4754
SmaI CCC'GGG
Cuts at: 976 1024 1169 1776 976
Size: 48 145 607 3954
Fragments arranged by size:
3954 607 145 48
SmlI C'TyrA_G
Cuts at: 1127 1243 1909 2824 3316 3578 1127
Size: 116 666 915 492 262 2303
Fragments arranged by size:
2303 915 666 492 262 116
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4754
SphI G_CATG'C
Cuts at: 1807 2307 1807
Size: 500 4254
StyI C'CwwG_G
Cuts at: 107 116 299 487 999 1050 1888 2242 107
Size: 9 183 188 512 51 838 354 2619
Fragments arranged by size:
2619 838 512 354 188 183 51 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1331 2337 2470 2929 3138 1331
Size: 1006 133 459 209 2947
Fragments arranged by size:
2947 1006 459 209 133
TatI w'GTAC_w
Cuts at: 268 401 446 4269 268
Size: 133 45 3823 753
Fragments arranged by size:
3823 753 133 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 799 831 1046 1078 4271 4303 704
Size: 32 63 32 215 32 3193 32 1155
Fragments arranged by size:
3193 1155 215 63 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2551 2551
Size: 4754
XbaI T'CTAG_A
Cuts at: 1785 1785
Size: 4754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 131 532 558 1245 2249 114
Size: 17 401 26 687 1004 2619
Fragments arranged by size:
2619 1004 687 401 26 17
XhoI C'TCGA_G
Cuts at: 1127 1243 1127
Size: 116 4638
XmaI C'CCGG_G
Cuts at: 974 1022 1167 1774 974
Size: 48 145 607 3954
Fragments arranged by size:
3954 607 145 48
ZraI GAC'GTC
Cuts at: 308 308
Size: 4754
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI ApaI AscI BfrBI BmtI
BsiWI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PsrI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI