Restriction map for
pF1KB5954
(Circular) (Six-base) MAPSORT of: pF1KB5954.seq Check: 109 from: 1 to: 4757
pF1KB5954 4757 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1221 1221
Size: 4757
AccI GT'mk_AC
Cuts at: 1493 1612 1795 1493
Size: 119 183 4455
Fragments arranged by size:
4455 183 119
Acc65I G'GTAC_C
Cuts at: 1773 1773
Size: 4757
AclI AA'CG_TT
Cuts at: 4597 4597
Size: 4757
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 2582 3014 3761 4029 559
Size: 2023 432 747 268 1287
Fragments arranged by size:
2023 1287 747 432 268
AflIII A'CryG_T
Cuts at: 830 1496 1636 1712 2109 3213 4190 830
Size: 666 140 76 397 1104 977 1397
Fragments arranged by size:
1397 1104 977 666 397 140 76
AgeI A'CCGG_T
Cuts at: 1163 3145 1163
Size: 1982 2775
AleI CACnn'nnGTG
Cuts at: 1117 1117
Size: 4757
AlwNI CAG_nnn'CTG
Cuts at: 2062 3629 2062
Size: 1567 3190
ApaLI G'TGCA_C
Cuts at: 3527 3527
Size: 4757
ApoI r'AATT_y
Cuts at: 534 1348 1761 4110 4366 534
Size: 814 413 2349 256 925
Fragments arranged by size:
2349 925 814 413 256
AseI AT'TA_AT
Cuts at: 20 1400 20
Size: 1380 3377
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
AvaI C'yCGr_G
Cuts at: 344 1777 344
Size: 1433 3324
BamHI G'GATC_C
Cuts at: 1782 1782
Size: 4757
BanI G'GyrC_C
Cuts at: 949 1097 1773 2187 2435 2470 949
Size: 148 676 414 248 35 3236
Fragments arranged by size:
3236 676 414 248 148 35
BanII G_rGCy'C
Cuts at: 1238 1771 2801 1238
Size: 533 1030 3194
Fragments arranged by size:
3194 1030 533
BbeI G_GCGC'C
Cuts at: 2191 2439 2191
Size: 248 4509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1697 1731 1697
Size: 34 4723
BciVI GTATCCnnnnn_n'
Cuts at: 2655 3416 4757 2655
Size: 761 1341 2655
Fragments arranged by size:
2655 1341 761
BclI T'GATC_A
Cuts at: 764 2277 764
Size: 1513 3244
BglII A'GATC_T
Cuts at: 2272 4309 2272
Size: 2037 2720
BlpI GC'TnA_GC
Cuts at: 1869 1869
Size: 4757
Bme1580I G_kGCm'C
Cuts at: 2382 2475 3531 2382
Size: 93 1056 3608
Fragments arranged by size:
3608 1056 93
BmgBI CAC'GTC
Cuts at: 1497 1713 1497
Size: 216 4541
BmrI ACTGGGnnnn_n'
Cuts at: 1969 2050 2385 4529 1969
Size: 81 335 2144 2197
Fragments arranged by size:
2197 2144 335 81
BmtI G_CTAG'C
Cuts at: 118 118
Size: 4757
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3124 3124
Size: 4757
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 767 674
Size: 93 4664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1933 2812 3304 3602 1933
Size: 879 492 298 3088
Fragments arranged by size:
3088 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1015 4461 1015
Size: 3446 1311
BsaAI yAC'GTr
Cuts at: 243 1119 2110 243
Size: 876 991 2890
Fragments arranged by size:
2890 991 876
BsaBI GATnn'nnATC
Cuts at: 6 2271 4308 6
Size: 2265 2037 455
Fragments arranged by size:
2265 2037 455
BsaHI Gr'CG_yC
Cuts at: 2188 2436 3138 4634 2188
Size: 248 702 1496 2311
Fragments arranged by size:
2311 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1163 1946 2467 3145 3419 3566 3977 1163
Size: 783 521 678 274 147 411 1943
Fragments arranged by size:
1943 783 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1426 1456 1426
Size: 30 4727
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 764 787 836 905 764
Size: 23 49 69 4616
Fragments arranged by size:
4616 69 49 23
BseYI C'CCAG_C
Cuts at: 503 1032 2182 2940 3517 4013 503
Size: 529 1150 758 577 496 1247
Fragments arranged by size:
1247 1150 758 577 529 496
BsiEI CG_ry'CG
Cuts at: 104 2299 2345 3553 104
Size: 2195 46 1208 1308
Fragments arranged by size:
2195 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1238 1771 2549 2739 3531 1238
Size: 533 778 190 792 2464
Fragments arranged by size:
2464 792 778 533 190
BsmI GAATG_Cn'
Cuts at: 662 662
Size: 4757
BsmBI CGTCTCn'nnnn_
Cuts at: 135 1703 135
Size: 1568 3189
Bsp1286I G_dGCh'C
Cuts at: 1238 1771 2382 2475 2549 2739 2801 3531 1238
Size: 533 611 93 74 190 62 730 2464
Fragments arranged by size:
2464 730 611 533 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3977 3977
Size: 4757
BspHI T'CATG_A
Cuts at: 3933 3933
Size: 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1033 1221 1558 1807 2021 2323 2704 1033
Size: 188 337 249 214 302 381 3086
Fragments arranged by size:
3086 381 337 302 249 214 188
BsrBI CCG'CTC
Cuts at: 50 3049 3103 4587 4752 50
Size: 2999 54 1484 165 55
Fragments arranged by size:
2999 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2669 2669
Size: 4757
BsrFI r'CCGG_y
Cuts at: 1163 2755 2936 3145 1163
Size: 1592 181 209 2775
Fragments arranged by size:
2775 1592 209 181
BssSI C'ACGA_G
Cuts at: 3028 3386 3028
Size: 358 4399
BstAPI GCAn_nnn'nTGC
Cuts at: 2134 2134
Size: 4757
BstBI TT'CG_AA
Cuts at: 3118 3118
Size: 4757
BstEII G'GTnAC_C
Cuts at: 861 861
Size: 4757
BstYI r'GATC_y
Cuts at: 1 1782 2249 2272 2607 2853 3865 3951 3963
Size: 1781 467 23 335 246 1012 86 12
Cuts at: 3963 4309 1
Size: 346 449
Fragments arranged by size:
1781 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1613 1613
Size: 4757
Bsu36I CC'TnA_GG
Cuts at: 1466 1466
Size: 4757
BtgI C'CryG_G
Cuts at: 107 155 629 857 1644 107
Size: 48 474 228 787 3220
Fragments arranged by size:
3220 787 474 228 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 164 1473 2707 2888 3051 164
Size: 1309 1234 181 163 1870
Fragments arranged by size:
1870 1309 1234 181 163
BtsI GCAGTG_nn'
Cuts at: 645 653 2000 645
Size: 8 1347 3402
Fragments arranged by size:
3402 1347 8
DraI TTT'AAA
Cuts at: 186 1756 186
Size: 1570 3187
DraIII CAC_nnn'GTG
Cuts at: 869 869
Size: 4757
DrdI GACnn_nn'nnGTC
Cuts at: 787 2463 3321 787
Size: 1676 858 2223
Fragments arranged by size:
2223 1676 858
EaeI y'GGCC_r
Cuts at: 696 2342 2516 2907 2934 4279 696
Size: 1646 174 391 27 1345 1174
Fragments arranged by size:
1646 1345 1174 391 174 27
EagI C'GGCC_G
Cuts at: 2342 2342
Size: 4757
EarI CTCTTCn'nnn_
Cuts at: 328 372 964 977 2780 2990 328
Size: 44 592 13 1803 210 2095
Fragments arranged by size:
2095 1803 592 210 44 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 604 2965 3275 3421 4567 604
Size: 2361 310 146 1146 794
Fragments arranged by size:
2361 1146 794 310 146
EcoICRI GAG'CTC
Cuts at: 1236 1769 1236
Size: 533 4224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 674 767 2582 3014 3761 4029 559
Size: 115 93 1815 432 747 268 1287
Fragments arranged by size:
1815 1287 747 432 268 115 93
EcoNI CCTnn'n_nnAGG
Cuts at: 746 746
Size: 4757
EcoO109I rG'GnC_Cy
Cuts at: 1896 1896
Size: 4757
EcoRI G'AATT_C
Cuts at: 1761 1761
Size: 4757
EcoRV GAT'ATC
Cuts at: 1362 1362
Size: 4757
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4757
FspI TGC'GCA
Cuts at: 520 2538 520
Size: 2018 2739
HaeII r_GCGC'y
Cuts at: 2191 2439 3461 4440 2191
Size: 248 1022 979 2508
Fragments arranged by size:
2508 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 845 877 1346 1378 1426 1458 2599 2629 2631
Size: 32 469 32 48 32 1141 30 2
Cuts at: 2631 2661 845
Size: 30 2941
Fragments arranged by size:
2941 1141 469 48 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1494 1796 1494
Size: 302 4455
HindIII A'AGCT_T
Cuts at: 964 964
Size: 4757
KasI G'GCGC_C
Cuts at: 2187 2435 2187
Size: 248 4509
KpnI G_GTAC'C
Cuts at: 1777 1777
Size: 4757
MluI A'CGCG_T
Cuts at: 4190 4190
Size: 4757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 943 1148 3428 3612 943
Size: 205 2280 184 2088
Fragments arranged by size:
2280 2088 205 184
MscI TGG'CCA
Cuts at: 698 2518 698
Size: 1820 2937
MslI CAynn'nnrTG
Cuts at: 1117 2746 2873 1117
Size: 1629 127 3001
Fragments arranged by size:
3001 1629 127
MspA1I CmG'CkG
Cuts at: 1868 2182 2542 3555 3800 1868
Size: 314 360 1013 245 2825
Fragments arranged by size:
2825 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2938 2938
Size: 4757
NarI GG'CG_CC
Cuts at: 2188 2436 2188
Size: 248 4509
NcoI C'CATG_G
Cuts at: 107 857 1644 107
Size: 750 787 3220
Fragments arranged by size:
3220 787 750
NgoMIV G'CCGG_C
Cuts at: 2936 2936
Size: 4757
NheI G'CTAG_C
Cuts at: 114 114
Size: 4757
NspI r_CATG'y
Cuts at: 834 1455 1640 1810 2310 3217 834
Size: 621 185 170 500 907 2374
Fragments arranged by size:
2374 907 621 500 185 170
PasI CC'CwG_GG
Cuts at: 1038 1038
Size: 4757
PciI A'CATG_T
Cuts at: 830 1636 3213 830
Size: 806 1577 2374
Fragments arranged by size:
2374 1577 806
PmeI GTTT'AAAC
Cuts at: 1756 1756
Size: 4757
PmlI CAC'GTG
Cuts at: 1119 1119
Size: 4757
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 3896
Size: 32 4725
PsiI TTA'TAA
Cuts at: 1574 1574
Size: 4757
PstI C_TGCA'G
Cuts at: 1218 1804 2489 1218
Size: 586 685 3486
Fragments arranged by size:
3486 685 586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4757
PvuII CAG'CTG
Cuts at: 2182 2542 2182
Size: 360 4397
RsrII CG'GwC_CG
Cuts at: 2952 2952
Size: 4757
SacI G_AGCT'C
Cuts at: 1238 1771 1238
Size: 533 4224
SalI G'TCGA_C
Cuts at: 1492 1794 1492
Size: 302 4455
SapI GCTCTTCn'nnn_
Cuts at: 2780 2990 2780
Size: 210 4547
SbfI CC_TGCA'GG
Cuts at: 1218 1804 1218
Size: 586 4171
ScaI AGT'ACT
Cuts at: 4274 4274
Size: 4757
SexAI A'CCwGG_T
Cuts at: 865 865
Size: 4757
SfcI C'TryA_G
Cuts at: 33 1214 1800 2485 3478 3669 33
Size: 1181 586 685 993 191 1121
Fragments arranged by size:
1181 1121 993 685 586 191
SfoI GGC'GCC
Cuts at: 2189 2437 2189
Size: 248 4509
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4757
SmaI CCC'GGG
Cuts at: 346 1779 346
Size: 1433 3324
SmlI C'TyrA_G
Cuts at: 1912 2827 3319 3581 1912
Size: 915 492 262 3088
Fragments arranged by size:
3088 915 492 262
SnaBI TAC'GTA
Cuts at: 243 243
Size: 4757
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4757
SphI G_CATG'C
Cuts at: 1810 2310 1810
Size: 500 4257
SspI AAT'ATT
Cuts at: 1202 1202
Size: 4757
StyI C'CwwG_G
Cuts at: 107 170 857 1644 1891 2245 107
Size: 63 687 787 247 354 2619
Fragments arranged by size:
2619 787 687 354 247 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 152 2340 2473 2932 3141 152
Size: 2188 133 459 209 1768
Fragments arranged by size:
2188 1768 459 209 133
TatI w'GTAC_w
Cuts at: 1013 1109 4272 1013
Size: 96 3163 1498
Fragments arranged by size:
3163 1498 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 583 615 1114 1146 4274 4306 583
Size: 32 499 32 3128 32 1034
Fragments arranged by size:
3128 1034 499 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2554 2554
Size: 4757
XbaI T'CTAG_A
Cuts at: 1788 1788
Size: 4757
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1651 2252 1651
Size: 601 4156
XmaI C'CCGG_G
Cuts at: 344 1777 344
Size: 1433 3324
XmnI GAAnn'nnTTC
Cuts at: 227 227
Size: 4757
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PasI PciI PmeI PmlI PpiI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BglI Bpu10I BsgI
BsiWI BsrGI BssHII BstXI ClaI CspCI FseI FspAI
HpaI MfeI NdeI NotI NruI NsiI PacI PflMI
PfoI PpuMI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI StuI SwaI XhoI ZraI