Restriction map for
pF1KB5955
(Circular) (Six-base) MAPSORT of: pF1KB5955.seq Check: 8599 from: 1 to: 4586
pF1KB5955 4586 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1624 1624
Size: 4586
Acc65I G'GTAC_C
Cuts at: 1602 1602
Size: 4586
AclI AA'CG_TT
Cuts at: 1510 4426 1510
Size: 2916 1670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 895 1057 2411 2843 3590 3858 895
Size: 162 1354 432 747 268 1623
Fragments arranged by size:
1623 1354 747 432 268 162
AfeI AGC'GCT
Cuts at: 492 492
Size: 4586
AflIII A'CryG_T
Cuts at: 1938 3042 4019 1938
Size: 1104 977 2505
Fragments arranged by size:
2505 1104 977
AgeI A'CCGG_T
Cuts at: 2974 2974
Size: 4586
AhdI GACnn_n'nnGTC
Cuts at: 288 288
Size: 4586
AleI CACnn'nnGTG
Cuts at: 1182 1182
Size: 4586
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1078 1078
Size: 4586
AlwNI CAG_nnn'CTG
Cuts at: 1891 3458 1891
Size: 1567 3019
ApaLI G'TGCA_C
Cuts at: 3356 3356
Size: 4586
ApoI r'AATT_y
Cuts at: 1367 1590 3939 4195 1367
Size: 223 2349 256 1758
Fragments arranged by size:
2349 1758 256 223
AseI AT'TA_AT
Cuts at: 20 20
Size: 4586
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
AvaI C'yCGr_G
Cuts at: 773 1606 773
Size: 833 3753
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 834 867 834
Size: 33 4553
BamHI G'GATC_C
Cuts at: 1611 1611
Size: 4586
BanI G'GyrC_C
Cuts at: 477 1602 2016 2264 2299 477
Size: 1125 414 248 35 2764
Fragments arranged by size:
2764 1125 414 248 35
BanII G_rGCy'C
Cuts at: 986 1460 1600 2630 986
Size: 474 140 1030 2942
Fragments arranged by size:
2942 1030 474 140
BbeI G_GCGC'C
Cuts at: 2020 2268 2020
Size: 248 4338
BbsI GAAGACnn'nnnn_
Cuts at: 172 934 172
Size: 762 3824
BciVI GTATCCnnnnn_n'
Cuts at: 2484 3245 4586 2484
Size: 761 1341 2484
Fragments arranged by size:
2484 1341 761
BclI T'GATC_A
Cuts at: 2106 2106
Size: 4586
BglII A'GATC_T
Cuts at: 2101 4138 2101
Size: 2037 2549
BlpI GC'TnA_GC
Cuts at: 1698 1698
Size: 4586
Bme1580I G_kGCm'C
Cuts at: 2211 2304 3360 2211
Size: 93 1056 3437
Fragments arranged by size:
3437 1056 93
BmgBI CAC'GTC
Cuts at: 1398 1398
Size: 4586
BmrI ACTGGGnnnn_n'
Cuts at: 1798 1879 2214 4358 1798
Size: 81 335 2144 2026
Fragments arranged by size:
2144 2026 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4586
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 515 1262 343
Size: 172 747 3667
Fragments arranged by size:
3667 747 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 1762 2641 3133 3431 162
Size: 1600 879 492 298 1317
Fragments arranged by size:
1600 1317 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1173 1497 4290 1173
Size: 324 2793 1469
Fragments arranged by size:
2793 1469 324
BsaAI yAC'GTr
Cuts at: 1939 1939
Size: 4586
BsaBI GATnn'nnATC
Cuts at: 6 2100 4137 6
Size: 2094 2037 455
Fragments arranged by size:
2094 2037 455
BsaHI Gr'CG_yC
Cuts at: 2017 2265 2967 4463 2017
Size: 248 702 1496 2140
Fragments arranged by size:
2140 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1775 2296 2974 3248 3395 3806 1775
Size: 521 678 274 147 411 2555
Fragments arranged by size:
2555 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 230 260 230
Size: 30 4556
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 340 994 340
Size: 654 3932
BseYI C'CCAG_C
Cuts at: 385 808 2011 2769 3346 3842 385
Size: 423 1203 758 577 496 1129
Fragments arranged by size:
1203 1129 758 577 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 720 720
Size: 4586
BsiEI CG_ry'CG
Cuts at: 104 2128 2174 3382 104
Size: 2024 46 1208 1308
Fragments arranged by size:
2024 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1460 1600 2378 2568 3360 1460
Size: 140 778 190 792 2686
Fragments arranged by size:
2686 792 778 190 140
Bsp1286I G_dGCh'C
Cuts at: 986 1460 1600 2211 2304 2378 2568 2630 3360
Size: 474 140 611 93 74 190 62 730
Cuts at: 3360 986
Size: 2212
Fragments arranged by size:
2212 730 611 474 190 140 93 74
62
BspEI T'CCGG_A
Cuts at: 3806 3806
Size: 4586
BspHI T'CATG_A
Cuts at: 923 3762 923
Size: 2839 1747
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1154 1636 1850 2152 2533 1154
Size: 482 214 302 381 3207
Fragments arranged by size:
3207 482 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1122 2878 2932 4416 4581 50
Size: 1072 1756 54 1484 165 55
Fragments arranged by size:
1756 1484 1072 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2498 2498
Size: 4586
BsrFI r'CCGG_y
Cuts at: 2584 2765 2974 2584
Size: 181 209 4196
Fragments arranged by size:
4196 209 181
BssSI C'ACGA_G
Cuts at: 949 2857 3215 949
Size: 1908 358 2320
Fragments arranged by size:
2320 1908 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1963 1963
Size: 4586
BstBI TT'CG_AA
Cuts at: 2947 2947
Size: 4586
BstEII G'GTnAC_C
Cuts at: 969 969
Size: 4586
BstXI CCAn_nnnn'nTGG
Cuts at: 789 789
Size: 4586
BstYI r'GATC_y
Cuts at: 1 222 767 1611 2078 2101 2436 2682 3694
Size: 221 545 844 467 23 335 246 1012
Cuts at: 3694 3780 3792 4138 1
Size: 86 12 346 449
Fragments arranged by size:
1012 844 545 467 449 346 335 246
221 86 23 12
BtgI C'CryG_G
Cuts at: 107 745 1553 107
Size: 638 808 3140
Fragments arranged by size:
3140 808 638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1058 2536 2717 2880 1058
Size: 1478 181 163 2764
Fragments arranged by size:
2764 1478 181 163
BtsI GCAGTG_nn'
Cuts at: 1829 1829
Size: 4586
DraI TTT'AAA
Cuts at: 1585 1585
Size: 4586
DrdI GACnn_nn'nnGTC
Cuts at: 1410 2292 3150 1410
Size: 882 858 2846
Fragments arranged by size:
2846 882 858
EaeI y'GGCC_r
Cuts at: 398 1010 2171 2345 2736 2763 4108 398
Size: 612 1161 174 391 27 1345 876
Fragments arranged by size:
1345 1161 876 612 391 174 27
EagI C'GGCC_G
Cuts at: 2171 2171
Size: 4586
EarI CTCTTCn'nnn_
Cuts at: 419 1033 1493 2609 2819 419
Size: 614 460 1116 210 2186
Fragments arranged by size:
2186 1116 614 460 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2794 3104 3250 4396 2794
Size: 310 146 1146 2984
Fragments arranged by size:
2984 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1458 1598 1458
Size: 140 4446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 515 895 1057 1262 2411 2843 3590 3858
Size: 172 380 162 205 1149 432 747 268
Cuts at: 3858 343
Size: 1071
Fragments arranged by size:
1149 1071 747 432 380 268 205 172
162
EcoNI CCTnn'n_nnAGG
Cuts at: 1414 1414
Size: 4586
EcoO109I rG'GnC_Cy
Cuts at: 1143 1725 1143
Size: 582 4004
EcoRI G'AATT_C
Cuts at: 1590 1590
Size: 4586
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1081 2605 1081
Size: 1524 3062
FspI TGC'GCA
Cuts at: 2367 2367
Size: 4586
HaeII r_GCGC'y
Cuts at: 494 809 2020 2268 3290 4269 494
Size: 315 1211 248 1022 979 811
Fragments arranged by size:
1211 1022 979 811 315 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 274 306 1309 1341 2428 2458 2460 2490 274
Size: 32 1003 32 1087 30 2 30 2370
Fragments arranged by size:
2370 1087 1003 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1625 1625
Size: 4586
HindIII A'AGCT_T
Cuts at: 534 534
Size: 4586
KasI G'GCGC_C
Cuts at: 2016 2264 2016
Size: 248 4338
KpnI G_GTAC'C
Cuts at: 1606 1606
Size: 4586
MluI A'CGCG_T
Cuts at: 4019 4019
Size: 4586
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3257 3441 3257
Size: 184 4402
MscI TGG'CCA
Cuts at: 400 1012 2347 400
Size: 612 1335 2639
Fragments arranged by size:
2639 1335 612
MslI CAynn'nnrTG
Cuts at: 575 1182 2575 2702 575
Size: 607 1393 127 2459
Fragments arranged by size:
2459 1393 607 127
MspA1I CmG'CkG
Cuts at: 1697 2011 2371 3384 3629 1697
Size: 314 360 1013 245 2654
Fragments arranged by size:
2654 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2767 2767
Size: 4586
NarI GG'CG_CC
Cuts at: 2017 2265 2017
Size: 248 4338
NcoI C'CATG_G
Cuts at: 107 745 107
Size: 638 3948
NgoMIV G'CCGG_C
Cuts at: 2765 2765
Size: 4586
NspI r_CATG'y
Cuts at: 1639 2139 3046 1639
Size: 500 907 3179
Fragments arranged by size:
3179 907 500
PasI CC'CwG_GG
Cuts at: 1378 1416 1378
Size: 38 4548
PciI A'CATG_T
Cuts at: 3042 3042
Size: 4586
PfoI T'CCnGG_A
Cuts at: 320 905 1532 320
Size: 585 627 3374
Fragments arranged by size:
3374 627 585
PmeI GTTT'AAAC
Cuts at: 1585 1585
Size: 4586
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3725 3757 3725
Size: 32 4554
PpuMI rG'GwC_Cy
Cuts at: 1143 1143
Size: 4586
PstI C_TGCA'G
Cuts at: 938 1633 2318 938
Size: 695 685 3206
Fragments arranged by size:
3206 695 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4586
PvuII CAG'CTG
Cuts at: 2011 2371 2011
Size: 360 4226
RsrII CG'GwC_CG
Cuts at: 2781 2781
Size: 4586
SacI G_AGCT'C
Cuts at: 1460 1600 1460
Size: 140 4446
SalI G'TCGA_C
Cuts at: 1623 1623
Size: 4586
SapI GCTCTTCn'nnn_
Cuts at: 1033 2609 2819 1033
Size: 1576 210 2800
Fragments arranged by size:
2800 1576 210
SbfI CC_TGCA'GG
Cuts at: 1633 1633
Size: 4586
ScaI AGT'ACT
Cuts at: 4103 4103
Size: 4586
SfcI C'TryA_G
Cuts at: 33 934 1422 1629 2314 3307 3498 33
Size: 901 488 207 685 993 191 1121
Fragments arranged by size:
1121 993 901 685 488 207 191
SfoI GGC'GCC
Cuts at: 2018 2266 2018
Size: 248 4338
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4586
SmaI CCC'GGG
Cuts at: 775 1608 775
Size: 833 3753
SmlI C'TyrA_G
Cuts at: 141 1741 2656 3148 3410 141
Size: 1600 915 492 262 1317
Fragments arranged by size:
1600 1317 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4586
SphI G_CATG'C
Cuts at: 1639 2139 1639
Size: 500 4086
StyI C'CwwG_G
Cuts at: 107 745 854 1013 1720 2074 107
Size: 638 109 159 707 354 2619
Fragments arranged by size:
2619 707 638 354 159 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2169 2302 2761 2970 2169
Size: 133 459 209 3785
Fragments arranged by size:
3785 459 209 133
TatI w'GTAC_w
Cuts at: 1004 1232 4101 1004
Size: 228 2869 1489
Fragments arranged by size:
2869 1489 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 230 262 1419 1451 4103 4135 230
Size: 32 1157 32 2652 32 681
Fragments arranged by size:
2652 1157 681 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2383 2383
Size: 4586
XbaI T'CTAG_A
Cuts at: 1617 1617
Size: 4586
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 743 2081 393
Size: 350 1338 2898
Fragments arranged by size:
2898 1338 350
XmaI C'CCGG_G
Cuts at: 773 1606 773
Size: 833 3753
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AloI ApaI AscI AvrII BbvCI
BcgI BfrBI BglI BmtI Bpu10I BsiWI BsmI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI