Restriction map for
pF1KB5957
(Circular) (Six-base) MAPSORT of: pF1KB5957.seq Check: 1764 from: 1 to: 4652
pF1KB5957 4652 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 160 160
Size: 4652
AatII G_ACGT'C
Cuts at: 588 588
Size: 4652
AccI GT'mk_AC
Cuts at: 1690 1690
Size: 4652
Acc65I G'GTAC_C
Cuts at: 1668 1668
Size: 4652
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 1512 2477 2909 3656 3924 436
Size: 1076 965 432 747 268 1164
Fragments arranged by size:
1164 1076 965 747 432 268
AflIII A'CryG_T
Cuts at: 257 2004 3108 4085 257
Size: 1747 1104 977 824
Fragments arranged by size:
1747 1104 977 824
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 4652
AhdI GACnn_n'nnGTC
Cuts at: 974 974
Size: 4652
AlwNI CAG_nnn'CTG
Cuts at: 1146 1957 3524 1146
Size: 811 1567 2274
Fragments arranged by size:
2274 1567 811
ApaI G_GGCC'C
Cuts at: 374 594 374
Size: 220 4432
ApaLI G'TGCA_C
Cuts at: 3422 3422
Size: 4652
ApoI r'AATT_y
Cuts at: 1656 4005 4261 1656
Size: 2349 256 2047
Fragments arranged by size:
2349 2047 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 502 1159 1542 1672 502
Size: 657 383 130 3482
Fragments arranged by size:
3482 657 383 130
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4652
BanI G'GyrC_C
Cuts at: 314 757 1668 2082 2330 2365 314
Size: 443 911 414 248 35 2601
Fragments arranged by size:
2601 911 443 414 248 35
BanII G_rGCy'C
Cuts at: 185 374 413 594 848 1195 1365 1666 2696
Size: 189 39 181 254 347 170 301 1030
Cuts at: 2696 185
Size: 2141
Fragments arranged by size:
2141 1030 347 301 254 189 181 170
39
BbeI G_GCGC'C
Cuts at: 2086 2334 2086
Size: 248 4404
BbsI GAAGACnn'nnnn_
Cuts at: 1280 1280
Size: 4652
BbvCI CC'TCA_GC
Cuts at: 793 1305 793
Size: 512 4140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 682 716 786 820 682
Size: 34 70 34 4514
Fragments arranged by size:
4514 70 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3311 4652 2550
Size: 761 1341 2550
Fragments arranged by size:
2550 1341 761
BclI T'GATC_A
Cuts at: 1568 2172 1568
Size: 604 4048
BglI GCCn_nnn'nGGC
Cuts at: 312 369 1518 312
Size: 57 1149 3446
Fragments arranged by size:
3446 1149 57
BglII A'GATC_T
Cuts at: 2167 4204 2167
Size: 2037 2615
BlpI GC'TnA_GC
Cuts at: 809 1764 809
Size: 955 3697
Bme1580I G_kGCm'C
Cuts at: 244 268 374 594 2277 2370 3426 244
Size: 24 106 220 1683 93 1056 1470
Fragments arranged by size:
1683 1470 1056 220 106 93 24
BmgBI CAC'GTC
Cuts at: 1059 1059
Size: 4652
BmrI ACTGGGnnnn_n'
Cuts at: 762 1864 1945 2280 4424 762
Size: 1102 81 335 2144 990
Fragments arranged by size:
2144 1102 990 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 634 3019 634
Size: 2385 2267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 902 1390 902
Size: 488 4164
Bpu10I CC'TnA_GC
Cuts at: 793 1305 793
Size: 512 4140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 739 1828 2707 3199 3497 642
Size: 97 1089 879 492 298 1797
Fragments arranged by size:
1797 1089 879 492 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 139 274 1452 1630 4356 139
Size: 135 1178 178 2726 435
Fragments arranged by size:
2726 1178 435 178 135
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 445 2166 4203 6
Size: 439 1721 2037 455
Fragments arranged by size:
2037 1721 455 439
BsaHI Gr'CG_yC
Cuts at: 585 2083 2331 3033 4529 585
Size: 1498 248 702 1496 708
Fragments arranged by size:
1498 1496 708 702 248
BsaWI w'CCGG_w
Cuts at: 1841 2362 3040 3314 3461 3872 1841
Size: 521 678 274 147 411 2621
Fragments arranged by size:
2621 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 682 712 682
Size: 30 4622
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 445 520 736 1553 445
Size: 75 216 817 3544
Fragments arranged by size:
3544 817 216 75
BseYI C'CCAG_C
Cuts at: 236 1087 2077 2835 3412 3908 236
Size: 851 990 758 577 496 980
Fragments arranged by size:
990 980 851 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1577 1305
Size: 272 4380
BsiEI CG_ry'CG
Cuts at: 104 364 465 2194 2240 3448 104
Size: 260 101 1729 46 1208 1308
Fragments arranged by size:
1729 1308 1208 260 101 46
BsiHKAI G_wGCw'C
Cuts at: 479 848 1666 2444 2634 3426 479
Size: 369 818 778 190 792 1705
Fragments arranged by size:
1705 818 792 778 369 190
BsmBI CGTCTCn'nnnn_
Cuts at: 576 576
Size: 4652
Bsp1286I G_dGCh'C
Cuts at: 185 244 268 374 413 479 594 848 1195
Size: 59 24 106 39 66 115 254 347
Cuts at: 1195 1365 1666 2277 2370 2444 2634 2696 3426
Size: 170 301 611 93 74 190 62 730
Cuts at: 3426 185
Size: 1411
Fragments arranged by size:
1411 730 611 347 301 254 190 170
115 106 93 74 66 62 59 39
24
BspEI T'CCGG_A
Cuts at: 3872 3872
Size: 4652
BspHI T'CATG_A
Cuts at: 3828 3828
Size: 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 160 1154 1702 1916 2218 2599 160
Size: 994 548 214 302 381 2213
Fragments arranged by size:
2213 994 548 381 302 214
BsrBI CCG'CTC
Cuts at: 50 360 467 501 1404 2944 2998 4482 4647
Size: 310 107 34 903 1540 54 1484 165
Cuts at: 4647 50
Size: 55
Fragments arranged by size:
1540 1484 903 310 165 107 55 54
34
BsrDI GCAATG_nn'
Cuts at: 2564 2564
Size: 4652
BsrFI r'CCGG_y
Cuts at: 311 2650 2831 3040 311
Size: 2339 181 209 1923
Fragments arranged by size:
2339 1923 209 181
BssSI C'ACGA_G
Cuts at: 608 2923 3281 608
Size: 2315 358 1979
Fragments arranged by size:
2315 1979 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2029 2029
Size: 4652
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 453 453
Size: 4652
BstYI r'GATC_y
Cuts at: 1 1677 2144 2167 2502 2748 3760 3846 3858
Size: 1676 467 23 335 246 1012 86 12
Cuts at: 3858 4204 1
Size: 346 449
Fragments arranged by size:
1676 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 480 1631 107
Size: 373 1151 3128
Fragments arranged by size:
3128 1151 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1191 2602 2783 2946 1191
Size: 1411 181 163 2897
Fragments arranged by size:
2897 1411 181 163
BtsI GCAGTG_nn'
Cuts at: 277 1337 1895 277
Size: 1060 558 3034
Fragments arranged by size:
3034 1060 558
DraI TTT'AAA
Cuts at: 557 575 1651 557
Size: 18 1076 3558
Fragments arranged by size:
3558 1076 18
DraIII CAC_nnn'GTG
Cuts at: 492 492
Size: 4652
DrdI GACnn_nn'nnGTC
Cuts at: 258 2358 3216 258
Size: 2100 858 1694
Fragments arranged by size:
2100 1694 858
EaeI y'GGCC_r
Cuts at: 125 201 320 361 483 734 1605 2237 2411
Size: 76 119 41 122 251 871 632 174
Cuts at: 2411 2802 2829 4174 125
Size: 391 27 1345 603
Fragments arranged by size:
1345 871 632 603 391 251 174 122
119 76 41 27
EagI C'GGCC_G
Cuts at: 361 2237 361
Size: 1876 2776
EarI CTCTTCn'nnn_
Cuts at: 574 812 2675 2885 574
Size: 238 1863 210 2341
Fragments arranged by size:
2341 1863 238 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2860 3170 3316 4462 2860
Size: 310 146 1146 3050
Fragments arranged by size:
3050 1146 310 146
EcoICRI GAG'CTC
Cuts at: 846 1664 846
Size: 818 3834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 436 902 1390 1512 2477 2909 3656 3924 436
Size: 466 488 122 965 432 747 268 1164
Fragments arranged by size:
1164 965 747 488 466 432 268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 139 1138 139
Size: 999 3653
EcoO109I rG'GnC_Cy
Cuts at: 370 1143 1791 370
Size: 773 648 3231
Fragments arranged by size:
3231 773 648
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4652
FspI TGC'GCA
Cuts at: 352 2433 352
Size: 2081 2571
HaeII r_GCGC'y
Cuts at: 2086 2334 3356 4335 2086
Size: 248 1022 979 2403
Fragments arranged by size:
2403 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 900 932 960 992 1155 1187 2494
Size: 32 519 32 28 32 163 32 1307
Cuts at: 2494 2524 2526 2556 349
Size: 30 2 30 2445
Fragments arranged by size:
2445 1307 519 163 32 32 32 32
30 30 28 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4652
KasI G'GCGC_C
Cuts at: 2082 2330 2082
Size: 248 4404
KpnI G_GTAC'C
Cuts at: 1672 1672
Size: 4652
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1161 3323 3507 1161
Size: 2162 184 2306
Fragments arranged by size:
2306 2162 184
MscI TGG'CCA
Cuts at: 322 736 1607 2413 322
Size: 414 871 806 2561
Fragments arranged by size:
2561 871 806 414
MslI CAynn'nnrTG
Cuts at: 451 2641 2768 451
Size: 2190 127 2335
Fragments arranged by size:
2335 2190 127
MspA1I CmG'CkG
Cuts at: 233 348 482 672 1410 1763 2077 2437 3450
Size: 115 134 190 738 353 314 360 1013
Cuts at: 3450 3695 233
Size: 245 1190
Fragments arranged by size:
1190 1013 738 360 353 314 245 190
134 115
NaeI GCC'GGC
Cuts at: 2833 2833
Size: 4652
NarI GG'CG_CC
Cuts at: 2083 2331 2083
Size: 248 4404
NcoI C'CATG_G
Cuts at: 107 1631 107
Size: 1524 3128
NgoMIV G'CCGG_C
Cuts at: 2831 2831
Size: 4652
NspI r_CATG'y
Cuts at: 303 1705 2205 3112 303
Size: 1402 500 907 1843
Fragments arranged by size:
1843 1402 907 500
PasI CC'CwG_GG
Cuts at: 594 594
Size: 4652
PciI A'CATG_T
Cuts at: 3108 3108
Size: 4652
PflMI CCAn_nnn'nTGG
Cuts at: 1413 1413
Size: 4652
PfoI T'CCnGG_A
Cuts at: 115 115
Size: 4652
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4620
PpuMI rG'GwC_Cy
Cuts at: 1143 1143
Size: 4652
PspOMI G'GGCC_C
Cuts at: 370 590 370
Size: 220 4432
PspXI vC'TCGA_Gb
Cuts at: 502 502
Size: 4652
PstI C_TGCA'G
Cuts at: 140 170 854 1699 2384 140
Size: 30 684 845 685 2408
Fragments arranged by size:
2408 845 685 684 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 348 672 2077 2437 348
Size: 324 1405 360 2563
Fragments arranged by size:
2563 1405 360 324
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4652
SacI G_AGCT'C
Cuts at: 848 1666 848
Size: 818 3834
SacII CC_GC'GG
Cuts at: 483 483
Size: 4652
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4442
SbfI CC_TGCA'GG
Cuts at: 854 1699 854
Size: 845 3807
ScaI AGT'ACT
Cuts at: 172 4169 172
Size: 3997 655
SfcI C'TryA_G
Cuts at: 33 136 166 850 1695 2380 3373 3564 33
Size: 103 30 684 845 685 993 191 1121
Fragments arranged by size:
1121 993 845 685 684 191 103 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 369 1518 369
Size: 1149 3503
SfoI GGC'GCC
Cuts at: 2084 2332 2084
Size: 248 4404
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 1674 1674
Size: 4652
SmlI C'TyrA_G
Cuts at: 502 621 718 1807 2722 3214 3476 502
Size: 119 97 1089 915 492 262 1678
Fragments arranged by size:
1678 1089 915 492 262 119 97
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4652
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
SspI AAT'ATT
Cuts at: 1357 1357
Size: 4652
StyI C'CwwG_G
Cuts at: 107 904 1631 1786 2140 107
Size: 797 727 155 354 2619
Fragments arranged by size:
2619 797 727 354 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 781 2235 2368 2827 3036 781
Size: 1454 133 459 209 2397
Fragments arranged by size:
2397 1454 459 209 133
TatI w'GTAC_w
Cuts at: 170 422 4167 170
Size: 252 3745 655
Fragments arranged by size:
3745 655 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1248 1280 4169 4201 1248
Size: 32 2889 32 1699
Fragments arranged by size:
2889 1699 32 32
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 2147 315
Size: 1832 2820
XhoI C'TCGA_G
Cuts at: 502 502
Size: 4652
XmaI C'CCGG_G
Cuts at: 1672 1672
Size: 4652
XmnI GAAnn'nnTTC
Cuts at: 1321 1321
Size: 4652
ZraI GAC'GTC
Cuts at: 586 586
Size: 4652
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AflII AleI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PmlI
PshAI PsiI PsrI SanDI SexAI SgrAI SnaBI SrfI
StuI SwaI