Restriction map for
pF1KB5959
(Circular) (Six-base) MAPSORT of: pF1KB5959.seq Check: 6909 from: 1 to: 4652
pF1KB5959 4652 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 312 1488 312
Size: 1176 3476
AatII G_ACGT'C
Cuts at: 634 634
Size: 4652
AccI GT'mk_AC
Cuts at: 1690 1690
Size: 4652
Acc65I G'GTAC_C
Cuts at: 1668 1668
Size: 4652
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1111 1440 2477 2909 3656 3924 301
Size: 810 329 1037 432 747 268 1029
Fragments arranged by size:
1037 1029 810 747 432 329 268
AfeI AGC'GCT
Cuts at: 1636 1636
Size: 4652
AflII C'TTAA_G
Cuts at: 292 292
Size: 4652
AflIII A'CryG_T
Cuts at: 1005 2004 3108 4085 1005
Size: 999 1104 977 1572
Fragments arranged by size:
1572 1104 999 977
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 4652
AleI CACnn'nnGTG
Cuts at: 770 1081 770
Size: 311 4341
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 994 994
Size: 4652
AlwNI CAG_nnn'CTG
Cuts at: 444 537 1144 1300 1344 1480 1957 3524 444
Size: 93 607 156 44 136 477 1567 1572
Fragments arranged by size:
1572 1567 607 477 156 136 93 44
ApaI G_GGCC'C
Cuts at: 191 496 1524 191
Size: 305 1028 3319
Fragments arranged by size:
3319 1028 305
ApaLI G'TGCA_C
Cuts at: 375 1602 3422 375
Size: 1227 1820 1605
Fragments arranged by size:
1820 1605 1227
ApoI r'AATT_y
Cuts at: 1656 4005 4261 1656
Size: 2349 256 2047
Fragments arranged by size:
2349 2047 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4652
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 158 309 667 1517 1544 1672 158
Size: 151 358 850 27 128 3138
Fragments arranged by size:
3138 850 358 151 128 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 351 384 351
Size: 33 4619
BamHI G'GATC_C
Cuts at: 1677 1677
Size: 4652
BanI G'GyrC_C
Cuts at: 507 1132 1531 1668 2082 2330 2365 507
Size: 625 399 137 414 248 35 2794
Fragments arranged by size:
2794 625 414 399 248 137 35
BanII G_rGCy'C
Cuts at: 159 191 496 524 531 1284 1466 1524 1666
Size: 32 305 28 7 753 182 58 142
Cuts at: 1666 2696 159
Size: 1030 2115
Fragments arranged by size:
2115 1030 753 305 182 142 58 32
28 7
BbeI G_GCGC'C
Cuts at: 2086 2334 2086
Size: 248 4404
BciVI GTATCCnnnnn_n'
Cuts at: 2550 3311 4652 2550
Size: 761 1341 2550
Fragments arranged by size:
2550 1341 761
BclI T'GATC_A
Cuts at: 861 1044 2172 861
Size: 183 1128 3341
Fragments arranged by size:
3341 1128 183
BfrBI ATG'CAT
Cuts at: 1333 1333
Size: 4652
BglII A'GATC_T
Cuts at: 2167 4204 2167
Size: 2037 2615
BlpI GC'TnA_GC
Cuts at: 1450 1764 1450
Size: 314 4338
Bme1580I G_kGCm'C
Cuts at: 191 379 496 1524 1606 2277 2370 3426 191
Size: 188 117 1028 82 671 93 1056 1417
Fragments arranged by size:
1417 1056 1028 671 188 117 93 82
BmgBI CAC'GTC
Cuts at: 1008 1008
Size: 4652
BmrI ACTGGGnnnn_n'
Cuts at: 167 1132 1224 1357 1864 1945 2280 4424 167
Size: 965 92 133 507 81 335 2144 395
Fragments arranged by size:
2144 965 507 395 335 133 92 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 207 3019 207
Size: 2812 1840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 538 540 789 454
Size: 84 2 249 4317
Fragments arranged by size:
4317 249 84 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1828 2707 3199 3497 1828
Size: 879 492 298 2983
Fragments arranged by size:
2983 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 868 4356 868
Size: 3488 1164
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 2166 4203 6
Size: 2160 2037 455
Fragments arranged by size:
2160 2037 455
BsaHI Gr'CG_yC
Cuts at: 129 631 2083 2331 3033 4529 129
Size: 502 1452 248 702 1496 252
Fragments arranged by size:
1496 1452 702 502 252 248
BsaWI w'CCGG_w
Cuts at: 1431 1436 1841 2362 3040 3314 3461 3872 1431
Size: 5 405 521 678 274 147 411 2211
Fragments arranged by size:
2211 678 521 411 405 274 147 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 146 176 457 487 768 798 146
Size: 30 281 30 281 30 4000
Fragments arranged by size:
4000 281 281 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 1075 481
Size: 594 4058
BseYI C'CCAG_C
Cuts at: 236 301 1177 1394 1466 2077 2835 3412 3908
Size: 65 876 217 72 611 758 577 496
Cuts at: 3908 236
Size: 980
Fragments arranged by size:
980 876 758 611 577 496 217 72
65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1204 1105
Size: 99 4553
BsiEI CG_ry'CG
Cuts at: 104 1447 2194 2240 3448 104
Size: 1343 747 46 1208 1308
Fragments arranged by size:
1343 1308 1208 747 46
BsiHKAI G_wGCw'C
Cuts at: 379 1589 1606 1666 2444 2634 3426 379
Size: 1210 17 60 778 190 792 1605
Fragments arranged by size:
1605 1210 792 778 190 60 17
BsmBI CGTCTCn'nnnn_
Cuts at: 558 558
Size: 4652
Bsp1286I G_dGCh'C
Cuts at: 159 191 379 496 524 531 1284 1466 1524
Size: 32 188 117 28 7 753 182 58
Cuts at: 1524 1589 1606 1666 2277 2370 2444 2634 2696
Size: 65 17 60 611 93 74 190 62
Cuts at: 2696 3426 159
Size: 730 1385
Fragments arranged by size:
1385 753 730 611 190 188 182 117
93 74 65 62 60 58 32 28
17 7
BspEI T'CCGG_A
Cuts at: 1436 3872 1436
Size: 2436 2216
BspHI T'CATG_A
Cuts at: 3828 3828
Size: 4652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 312 438 1488 1702 1916 2218 2599 312
Size: 126 1050 214 214 302 381 2365
Fragments arranged by size:
2365 1050 381 302 214 214 126
BsrBI CCG'CTC
Cuts at: 50 232 2944 2998 4482 4647 50
Size: 182 2712 54 1484 165 55
Fragments arranged by size:
2712 1484 182 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2564 2564
Size: 4652
BsrFI r'CCGG_y
Cuts at: 1442 1613 2650 2831 3040 1442
Size: 171 1037 181 209 3054
Fragments arranged by size:
3054 1037 209 181 171
BsrGI T'GTAC_A
Cuts at: 654 944 654
Size: 290 4362
BssSI C'ACGA_G
Cuts at: 2923 3281 2923
Size: 358 4294
BstAPI GCAn_nnn'nTGC
Cuts at: 444 1330 1610 2029 444
Size: 886 280 419 3067
Fragments arranged by size:
3067 886 419 280
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstEII G'GTnAC_C
Cuts at: 417 417
Size: 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 831 831
Size: 4652
BstYI r'GATC_y
Cuts at: 1 1358 1677 2144 2167 2502 2748 3760 3846
Size: 1357 319 467 23 335 246 1012 86
Cuts at: 3846 3858 4204 1
Size: 12 346 449
Fragments arranged by size:
1357 1012 467 449 346 335 319 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1275 1275
Size: 4652
BtgI C'CryG_G
Cuts at: 107 620 712 107
Size: 513 92 4047
Fragments arranged by size:
4047 513 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1498 2602 2783 2946 1498
Size: 1104 181 163 3204
Fragments arranged by size:
3204 1104 181 163
BtsI GCAGTG_nn'
Cuts at: 214 599 786 1895 214
Size: 385 187 1109 2971
Fragments arranged by size:
2971 1109 385 187
ClaI AT'CG_AT
Cuts at: 566 566
Size: 4652
DraI TTT'AAA
Cuts at: 1651 1651
Size: 4652
DrdI GACnn_nn'nnGTC
Cuts at: 636 2358 3216 636
Size: 1722 858 2072
Fragments arranged by size:
2072 1722 858
EaeI y'GGCC_r
Cuts at: 617 958 2237 2411 2802 2829 4174 617
Size: 341 1279 174 391 27 1345 1095
Fragments arranged by size:
1345 1279 1095 391 341 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4652
EarI CTCTTCn'nnn_
Cuts at: 1211 2675 2885 1211
Size: 1464 210 2978
Fragments arranged by size:
2978 1464 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1401 2860 3170 3316 4462 1401
Size: 1459 310 146 1146 1591
Fragments arranged by size:
1591 1459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1664 1664
Size: 4652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 454 538 540 789 1111 1440 2477 2909
Size: 153 84 2 249 322 329 1037 432
Cuts at: 2909 3656 3924 301
Size: 747 268 1029
Fragments arranged by size:
1037 1029 747 432 329 322 268 249
153 84 2
EcoNI CCTnn'n_nnAGG
Cuts at: 319 319
Size: 4652
EcoO109I rG'GnC_Cy
Cuts at: 169 187 188 492 493 534 1304 1791 169
Size: 18 1 304 1 41 770 487 3030
Fragments arranged by size:
3030 770 487 304 41 18 1 1
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4652
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4652
HaeII r_GCGC'y
Cuts at: 1638 2086 2334 3356 4335 1638
Size: 448 248 1022 979 1955
Fragments arranged by size:
1955 1022 979 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 1476 1508 2494 2524 2526 2556 396
Size: 32 1048 32 986 30 2 30 2492
Fragments arranged by size:
2492 1048 986 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4652
HindIII A'AGCT_T
Cuts at: 1452 1452
Size: 4652
KasI G'GCGC_C
Cuts at: 2082 2330 2082
Size: 248 4404
KpnI G_GTAC'C
Cuts at: 1672 1672
Size: 4652
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 566 3323 3507 566
Size: 2757 184 1711
Fragments arranged by size:
2757 1711 184
MscI TGG'CCA
Cuts at: 960 2413 960
Size: 1453 3199
MslI CAynn'nnrTG
Cuts at: 770 1081 2641 2768 770
Size: 311 1560 127 2654
Fragments arranged by size:
2654 1560 311 127
MspA1I CmG'CkG
Cuts at: 401 743 761 1089 1394 1763 2077 2437 3450
Size: 342 18 328 305 369 314 360 1013
Cuts at: 3450 3695 401
Size: 245 1358
Fragments arranged by size:
1358 1013 369 360 342 328 314 305
245 18
NaeI GCC'GGC
Cuts at: 2833 2833
Size: 4652
NarI GG'CG_CC
Cuts at: 2083 2331 2083
Size: 248 4404
NcoI C'CATG_G
Cuts at: 107 620 712 107
Size: 513 92 4047
Fragments arranged by size:
4047 513 92
NgoMIV G'CCGG_C
Cuts at: 2831 2831
Size: 4652
NsiI A_TGCA'T
Cuts at: 1335 1335
Size: 4652
NspI r_CATG'y
Cuts at: 1705 2205 3112 1705
Size: 500 907 3245
Fragments arranged by size:
3245 907 500
PasI CC'CwG_GG
Cuts at: 1144 1144
Size: 4652
PciI A'CATG_T
Cuts at: 3108 3108
Size: 4652
PflMI CCAn_nnn'nTGG
Cuts at: 537 967 1144 537
Size: 430 177 4045
Fragments arranged by size:
4045 430 177
PfoI T'CCnGG_A
Cuts at: 556 556
Size: 4652
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4620
PpuMI rG'GwC_Cy
Cuts at: 169 1304 169
Size: 1135 3517
PspOMI G'GGCC_C
Cuts at: 187 492 1520 187
Size: 305 1028 3319
Fragments arranged by size:
3319 1028 305
PstI C_TGCA'G
Cuts at: 449 460 491 646 1699 2384 449
Size: 11 31 155 1053 685 2717
Fragments arranged by size:
2717 1053 685 155 31 11
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 401 1089 1394 2077 2437 401
Size: 688 305 683 360 2616
Fragments arranged by size:
2616 688 683 360 305
RsrII CG'GwC_CG
Cuts at: 1434 2847 1434
Size: 1413 3239
SacI G_AGCT'C
Cuts at: 1666 1666
Size: 4652
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SanDI GG'GwC_CC
Cuts at: 169 169
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 1211 2675 2885 1211
Size: 1464 210 2978
Fragments arranged by size:
2978 1464 210
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4652
ScaI AGT'ACT
Cuts at: 4169 4169
Size: 4652
SfcI C'TryA_G
Cuts at: 33 445 456 487 543 642 1695 2380 3373
Size: 412 11 31 56 99 1053 685 993
Cuts at: 3373 3564 33
Size: 191 1121
Fragments arranged by size:
1121 1053 993 685 412 191 99 56
31 11
SfoI GGC'GCC
Cuts at: 2084 2332 2084
Size: 248 4404
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 160 669 1519 1674 160
Size: 509 850 155 3138
Fragments arranged by size:
3138 850 509 155
SmlI C'TyrA_G
Cuts at: 292 1807 2722 3214 3476 292
Size: 1515 915 492 262 1468
Fragments arranged by size:
1515 1468 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4652
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
StuI AGG'CCT
Cuts at: 1627 1627
Size: 4652
StyI C'CwwG_G
Cuts at: 107 132 281 620 712 1786 2140 107
Size: 25 149 339 92 1074 354 2619
Fragments arranged by size:
2619 1074 354 339 149 92 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 892 2235 2368 2827 3036 688
Size: 204 1343 133 459 209 2304
Fragments arranged by size:
2304 1343 459 209 204 133
TatI w'GTAC_w
Cuts at: 654 944 4167 654
Size: 290 3223 1139
Fragments arranged by size:
3223 1139 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 146 178 457 489 708 740 768 800 1585
Size: 32 279 32 219 32 28 32 785
Cuts at: 1585 1617 4169 4201 146
Size: 32 2552 32 597
Fragments arranged by size:
2552 785 597 279 219 32 32 32
32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 862 2147 862
Size: 1285 3367
XmaI C'CCGG_G
Cuts at: 158 667 1517 1672 158
Size: 509 850 155 3138
Fragments arranged by size:
3138 850 509 155
ZraI GAC'GTC
Cuts at: 632 632
Size: 4652
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AhdI AloI AscI AvrII BbsI BbvCI BcgI BglI
BmtI Bpu10I BsiWI BsmI BssHII BstZ17I CspCI DraIII
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI