Restriction map for
pF1KB5961
(Circular) (Six-base) MAPSORT of: pF1KB5961.seq Check: 7019 from: 1 to: 4658
pF1KB5961 4658 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1696 1696
Size: 4658
Acc65I G'GTAC_C
Cuts at: 1674 1674
Size: 4658
AclI AA'CG_TT
Cuts at: 4498 4498
Size: 4658
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1160 2483 2915 3662 3930 1160
Size: 1323 432 747 268 1888
Fragments arranged by size:
1888 1323 747 432 268
AflIII A'CryG_T
Cuts at: 2010 3114 4091 2010
Size: 1104 977 2577
Fragments arranged by size:
2577 1104 977
AgeI A'CCGG_T
Cuts at: 3046 3046
Size: 4658
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 145 1229 145
Size: 1084 3574
AlwNI CAG_nnn'CTG
Cuts at: 1963 3530 1963
Size: 1567 3091
ApaLI G'TGCA_C
Cuts at: 3428 3428
Size: 4658
ApoI r'AATT_y
Cuts at: 1662 4011 4267 1662
Size: 2349 256 2053
Fragments arranged by size:
2349 2053 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4658
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
AvaI C'yCGr_G
Cuts at: 1678 1678
Size: 4658
BamHI G'GATC_C
Cuts at: 1683 1683
Size: 4658
BanI G'GyrC_C
Cuts at: 172 1674 2088 2336 2371 172
Size: 1502 414 248 35 2459
Fragments arranged by size:
2459 1502 414 248 35
BanII G_rGCy'C
Cuts at: 190 1672 2702 190
Size: 1482 1030 2146
Fragments arranged by size:
2146 1482 1030
BbeI G_GCGC'C
Cuts at: 2092 2340 2092
Size: 248 4410
BciVI GTATCCnnnnn_n'
Cuts at: 2556 3317 4658 2556
Size: 761 1341 2556
Fragments arranged by size:
2556 1341 761
BclI T'GATC_A
Cuts at: 2178 2178
Size: 4658
BglI GCCn_nnn'nGGC
Cuts at: 163 661 163
Size: 498 4160
BglII A'GATC_T
Cuts at: 2173 4210 2173
Size: 2037 2621
BlpI GC'TnA_GC
Cuts at: 1770 1770
Size: 4658
Bme1580I G_kGCm'C
Cuts at: 2283 2376 3432 2283
Size: 93 1056 3509
Fragments arranged by size:
3509 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 635 1870 1951 2286 4430 635
Size: 1235 81 335 2144 863
Fragments arranged by size:
2144 1235 863 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 4658
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 577 230
Size: 347 4311
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1238 1834 2713 3205 3503 1238
Size: 596 879 492 298 2393
Fragments arranged by size:
2393 879 596 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4362 4362
Size: 4658
BsaAI yAC'GTr
Cuts at: 2011 2011
Size: 4658
BsaBI GATnn'nnATC
Cuts at: 6 2172 4209 6
Size: 2166 2037 455
Fragments arranged by size:
2166 2037 455
BsaHI Gr'CG_yC
Cuts at: 2089 2337 3039 4535 2089
Size: 248 702 1496 2212
Fragments arranged by size:
2212 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1847 2368 3046 3320 3467 3878 1847
Size: 521 678 274 147 411 2627
Fragments arranged by size:
2627 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 337 340 337
Size: 3 4655
BseYI C'CCAG_C
Cuts at: 158 2083 2841 3418 3914 158
Size: 1925 758 577 496 902
Fragments arranged by size:
1925 902 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2200 2246 3454 104
Size: 2096 46 1208 1308
Fragments arranged by size:
2096 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 450 1672 2450 2640 3432 450
Size: 1222 778 190 792 1676
Fragments arranged by size:
1676 1222 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 206 301 206
Size: 95 4563
Bsp1286I G_dGCh'C
Cuts at: 190 450 1672 2283 2376 2450 2640 2702 3432
Size: 260 1222 611 93 74 190 62 730
Cuts at: 3432 190
Size: 1416
Fragments arranged by size:
1416 1222 730 611 260 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3878 3878
Size: 4658
BspHI T'CATG_A
Cuts at: 875 3834 875
Size: 2959 1699
BspMI ACCTGCnnnn'nnnn_
Cuts at: 585 1708 1922 2224 2605 585
Size: 1123 214 302 381 2638
Fragments arranged by size:
2638 1123 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2950 3004 4488 4653 50
Size: 2900 54 1484 165 55
Fragments arranged by size:
2900 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2570 2570
Size: 4658
BsrFI r'CCGG_y
Cuts at: 2656 2837 3046 2656
Size: 181 209 4268
Fragments arranged by size:
4268 209 181
BssSI C'ACGA_G
Cuts at: 2929 3287 2929
Size: 358 4300
BstAPI GCAn_nnn'nTGC
Cuts at: 2035 2035
Size: 4658
BstBI TT'CG_AA
Cuts at: 3019 3019
Size: 4658
BstYI r'GATC_y
Cuts at: 1 985 1432 1683 2150 2173 2508 2754 3766
Size: 984 447 251 467 23 335 246 1012
Cuts at: 3766 3852 3864 4210 1
Size: 86 12 346 449
Fragments arranged by size:
1012 984 467 449 447 346 335 251
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 228 107
Size: 121 4537
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2608 2789 2952 2608
Size: 181 163 4314
Fragments arranged by size:
4314 181 163
BtsI GCAGTG_nn'
Cuts at: 589 709 935 1213 1901 589
Size: 120 226 278 688 3346
Fragments arranged by size:
3346 688 278 226 120
ClaI AT'CG_AT
Cuts at: 1386 1386
Size: 4658
DraI TTT'AAA
Cuts at: 855 992 1657 855
Size: 137 665 3856
Fragments arranged by size:
3856 665 137
DrdI GACnn_nn'nnGTC
Cuts at: 2364 3222 2364
Size: 858 3800
EaeI y'GGCC_r
Cuts at: 2243 2417 2808 2835 4180 2243
Size: 174 391 27 1345 2721
Fragments arranged by size:
2721 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2243 2243
Size: 4658
EarI CTCTTCn'nnn_
Cuts at: 1194 2681 2891 1194
Size: 1487 210 2961
Fragments arranged by size:
2961 1487 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2866 3176 3322 4468 2866
Size: 310 146 1146 3056
Fragments arranged by size:
3056 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1670 1670
Size: 4658
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 577 1160 2483 2915 3662 3930 230
Size: 347 583 1323 432 747 268 958
Fragments arranged by size:
1323 958 747 583 432 347 268
EcoO109I rG'GnC_Cy
Cuts at: 278 770 1249 1797 278
Size: 492 479 548 3139
Fragments arranged by size:
3139 548 492 479
EcoRI G'AATT_C
Cuts at: 1662 1662
Size: 4658
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4658
FspI TGC'GCA
Cuts at: 2439 2439
Size: 4658
HaeII r_GCGC'y
Cuts at: 2092 2340 3362 4341 2092
Size: 248 1022 979 2409
Fragments arranged by size:
2409 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 432 464 611 643 2500 2530 2532 2562 432
Size: 32 147 32 1857 30 2 30 2528
Fragments arranged by size:
2528 1857 147 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1697 1697
Size: 4658
HindIII A'AGCT_T
Cuts at: 1306 1306
Size: 4658
KasI G'GCGC_C
Cuts at: 2088 2336 2088
Size: 248 4410
KpnI G_GTAC'C
Cuts at: 1678 1678
Size: 4658
MluI A'CGCG_T
Cuts at: 4091 4091
Size: 4658
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 665 1318 3329 3513 665
Size: 653 2011 184 1810
Fragments arranged by size:
2011 1810 653 184
MscI TGG'CCA
Cuts at: 2419 2419
Size: 4658
MslI CAynn'nnrTG
Cuts at: 839 2647 2774 839
Size: 1808 127 2723
Fragments arranged by size:
2723 1808 127
MspA1I CmG'CkG
Cuts at: 275 345 1769 2083 2443 3456 3701 275
Size: 70 1424 314 360 1013 245 1232
Fragments arranged by size:
1424 1232 1013 360 314 245 70
NaeI GCC'GGC
Cuts at: 2839 2839
Size: 4658
NarI GG'CG_CC
Cuts at: 2089 2337 2089
Size: 248 4410
NcoI C'CATG_G
Cuts at: 107 107
Size: 4658
NgoMIV G'CCGG_C
Cuts at: 2837 2837
Size: 4658
NspI r_CATG'y
Cuts at: 1711 2211 3118 1711
Size: 500 907 3251
Fragments arranged by size:
3251 907 500
PciI A'CATG_T
Cuts at: 3114 3114
Size: 4658
PmeI GTTT'AAAC
Cuts at: 1657 1657
Size: 4658
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 3797
Size: 32 4626
PpuMI rG'GwC_Cy
Cuts at: 278 770 278
Size: 492 4166
PstI C_TGCA'G
Cuts at: 596 683 1705 2390 596
Size: 87 1022 685 2864
Fragments arranged by size:
2864 1022 685 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4658
PvuII CAG'CTG
Cuts at: 2083 2443 2083
Size: 360 4298
RsrII CG'GwC_CG
Cuts at: 2853 2853
Size: 4658
SacI G_AGCT'C
Cuts at: 1672 1672
Size: 4658
SalI G'TCGA_C
Cuts at: 1695 1695
Size: 4658
SapI GCTCTTCn'nnn_
Cuts at: 2681 2891 2681
Size: 210 4448
SbfI CC_TGCA'GG
Cuts at: 1705 1705
Size: 4658
ScaI AGT'ACT
Cuts at: 4175 4175
Size: 4658
SfcI C'TryA_G
Cuts at: 33 592 679 1166 1701 2386 3379 3570 33
Size: 559 87 487 535 685 993 191 1121
Fragments arranged by size:
1121 993 685 559 535 487 191 87
SfoI GGC'GCC
Cuts at: 2090 2338 2090
Size: 248 4410
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4658
SmaI CCC'GGG
Cuts at: 1680 1680
Size: 4658
SmlI C'TyrA_G
Cuts at: 1253 1813 2728 3220 3482 1253
Size: 560 915 492 262 2429
Fragments arranged by size:
2429 915 560 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4658
SphI G_CATG'C
Cuts at: 1711 2211 1711
Size: 500 4158
SspI AAT'ATT
Cuts at: 546 546
Size: 4658
StyI C'CwwG_G
Cuts at: 107 1792 2146 107
Size: 1685 354 2619
Fragments arranged by size:
2619 1685 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2241 2374 2833 3042 2241
Size: 133 459 209 3857
Fragments arranged by size:
3857 459 209 133
TatI w'GTAC_w
Cuts at: 382 4173 382
Size: 3791 867
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4175 4207 4175
Size: 32 4626
Tth111I GACn'n_nGTC
Cuts at: 2455 2455
Size: 4658
XbaI T'CTAG_A
Cuts at: 1689 1689
Size: 4658
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2153 2153
Size: 4658
XmaI C'CCGG_G
Cuts at: 1678 1678
Size: 4658
XmnI GAAnn'nnTTC
Cuts at: 812 812
Size: 4658
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI