Restriction map for
pF1KB5965
(Circular) (Six-base) MAPSORT of: pF1KB5965.seq Check: 3863 from: 1 to: 4841
pF1KB5965 4841 bp
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1417 1417
Size: 4841
AccI GT'mk_AC
Cuts at: 391 936 1375 1879 391
Size: 545 439 504 3353
Fragments arranged by size:
3353 545 504 439
Acc65I G'GTAC_C
Cuts at: 1857 1857
Size: 4841
AclI AA'CG_TT
Cuts at: 4681 4681
Size: 4841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1471 2666 3098 3845 4113 988
Size: 483 1195 432 747 268 1716
Fragments arranged by size:
1716 1195 747 483 432 268
AflIII A'CryG_T
Cuts at: 2193 3297 4274 2193
Size: 1104 977 2760
Fragments arranged by size:
2760 1104 977
AgeI A'CCGG_T
Cuts at: 3229 3229
Size: 4841
AhdI GACnn_n'nnGTC
Cuts at: 315 315
Size: 4841
AleI CACnn'nnGTG
Cuts at: 1211 1731 1211
Size: 520 4321
AlwNI CAG_nnn'CTG
Cuts at: 274 2146 3713 274
Size: 1872 1567 1402
Fragments arranged by size:
1872 1567 1402
ApaLI G'TGCA_C
Cuts at: 1232 1366 3611 1232
Size: 134 2245 2462
Fragments arranged by size:
2462 2245 134
ApoI r'AATT_y
Cuts at: 1845 4194 4450 1845
Size: 2349 256 2236
Fragments arranged by size:
2349 2236 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4841
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
AvaI C'yCGr_G
Cuts at: 680 721 1125 1861 680
Size: 41 404 736 3660
Fragments arranged by size:
3660 736 404 41
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1101 1134 1101
Size: 33 4808
BamHI G'GATC_C
Cuts at: 1019 1866 1019
Size: 847 3994
BanI G'GyrC_C
Cuts at: 794 1857 2271 2519 2554 794
Size: 1063 414 248 35 3081
Fragments arranged by size:
3081 1063 414 248 35
BanII G_rGCy'C
Cuts at: 1855 2885 1855
Size: 1030 3811
BbeI G_GCGC'C
Cuts at: 2275 2523 2275
Size: 248 4593
BciVI GTATCCnnnnn_n'
Cuts at: 1129 2739 3500 4841 1129
Size: 1610 761 1341 1129
Fragments arranged by size:
1610 1341 1129 761
BclI T'GATC_A
Cuts at: 2361 2361
Size: 4841
BglI GCCn_nnn'nGGC
Cuts at: 1600 1600
Size: 4841
BglII A'GATC_T
Cuts at: 2356 4393 2356
Size: 2037 2804
BlpI GC'TnA_GC
Cuts at: 950 1953 950
Size: 1003 3838
Bme1580I G_kGCm'C
Cuts at: 883 1066 1236 1370 2466 2559 3615 883
Size: 183 170 134 1096 93 1056 2109
Fragments arranged by size:
2109 1096 1056 183 170 134 93
BmgBI CAC'GTC
Cuts at: 479 479
Size: 4841
BmrI ACTGGGnnnn_n'
Cuts at: 1725 2053 2134 2469 4613 1725
Size: 328 81 335 2144 1953
Fragments arranged by size:
2144 1953 335 328 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3208 3208
Size: 4841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 1171 431
Size: 740 4101
Bpu10I CC'TnA_GC
Cuts at: 671 1559 1565 671
Size: 888 6 3947
Fragments arranged by size:
3947 888 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1526 1532 1556 2017 2896 3388 3686 1526
Size: 6 24 461 879 492 298 2681
Fragments arranged by size:
2681 879 492 461 298 24 6
BsaI GGTCTCn'nnnn_
Cuts at: 856 1036 4545 856
Size: 180 3509 1152
Fragments arranged by size:
3509 1152 180
BsaAI yAC'GTr
Cuts at: 2194 2194
Size: 4841
BsaBI GATnn'nnATC
Cuts at: 6 767 838 2355 4392 6
Size: 761 71 1517 2037 455
Fragments arranged by size:
2037 1517 761 455 71
BsaHI Gr'CG_yC
Cuts at: 2272 2520 3222 4718 2272
Size: 248 702 1496 2395
Fragments arranged by size:
2395 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2030 2551 3229 3503 3650 4061 2030
Size: 521 678 274 147 411 2810
Fragments arranged by size:
2810 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 923 1106 2266 3024 3601 4097 923
Size: 183 1160 758 577 496 1667
Fragments arranged by size:
1667 1160 758 577 496 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1395 1519 1395
Size: 124 4717
BsiEI CG_ry'CG
Cuts at: 104 2383 2429 3637 104
Size: 2279 46 1208 1308
Fragments arranged by size:
2279 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 808 1236 1370 1823 1855 2633 2823 3615 808
Size: 428 134 453 32 778 190 792 2034
Fragments arranged by size:
2034 792 778 453 428 190 134 32
Bsp1286I G_dGCh'C
Cuts at: 808 883 1066 1236 1370 1823 1855 2466 2559
Size: 75 183 170 134 453 32 611 93
Cuts at: 2559 2633 2823 2885 3615 808
Size: 74 190 62 730 2034
Fragments arranged by size:
2034 730 611 453 190 183 170 134
93 75 74 62 32
BspEI T'CCGG_A
Cuts at: 4061 4061
Size: 4841
BspHI T'CATG_A
Cuts at: 4017 4017
Size: 4841
BspMI ACCTGCnnnn'nnnn_
Cuts at: 573 1417 1891 2105 2407 2788 573
Size: 844 474 214 302 381 2626
Fragments arranged by size:
2626 844 474 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3133 3187 4671 4836 50
Size: 3083 54 1484 165 55
Fragments arranged by size:
3083 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 885 2753 885
Size: 1868 2973
BsrFI r'CCGG_y
Cuts at: 2839 3020 3229 2839
Size: 181 209 4451
Fragments arranged by size:
4451 209 181
BsrGI T'GTAC_A
Cuts at: 1403 1403
Size: 4841
BssSI C'ACGA_G
Cuts at: 3112 3470 3112
Size: 358 4483
BstAPI GCAn_nnn'nTGC
Cuts at: 1418 1497 2218 1418
Size: 79 721 4041
Fragments arranged by size:
4041 721 79
BstBI TT'CG_AA
Cuts at: 3202 3202
Size: 4841
BstXI CCAn_nnnn'nTGG
Cuts at: 1675 1815 1675
Size: 140 4701
BstYI r'GATC_y
Cuts at: 1 839 1019 1866 2333 2356 2691 2937 3949
Size: 838 180 847 467 23 335 246 1012
Cuts at: 3949 4035 4047 4393 1
Size: 86 12 346 449
Fragments arranged by size:
1012 847 838 467 449 346 335 246
180 86 23 12
BstZ17I GTA'TAC
Cuts at: 937 937
Size: 4841
Bsu36I CC'TnA_GG
Cuts at: 294 486 752 294
Size: 192 266 4383
Fragments arranged by size:
4383 266 192
BtgI C'CryG_G
Cuts at: 107 107
Size: 4841
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2791 2972 3135 2791
Size: 181 163 4497
Fragments arranged by size:
4497 181 163
BtsI GCAGTG_nn'
Cuts at: 666 1068 1529 2084 666
Size: 402 461 555 3423
Fragments arranged by size:
3423 555 461 402
DraI TTT'AAA
Cuts at: 1840 1840
Size: 4841
DraIII CAC_nnn'GTG
Cuts at: 1386 1386
Size: 4841
DrdI GACnn_nn'nnGTC
Cuts at: 739 2547 3405 739
Size: 1808 858 2175
Fragments arranged by size:
2175 1808 858
EaeI y'GGCC_r
Cuts at: 1826 2426 2600 2991 3018 4363 1826
Size: 600 174 391 27 1345 2304
Fragments arranged by size:
2304 1345 600 391 174 27
EagI C'GGCC_G
Cuts at: 2426 2426
Size: 4841
EarI CTCTTCn'nnn_
Cuts at: 432 563 2864 3074 432
Size: 131 2301 210 2199
Fragments arranged by size:
2301 2199 210 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 653 976 1159 1780 3049 3359 3505 4651 653
Size: 323 183 621 1269 310 146 1146 843
Fragments arranged by size:
1269 1146 843 621 323 310 183 146
EcoICRI GAG'CTC
Cuts at: 1853 1853
Size: 4841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 431 988 1171 1471 2666 3098 3845 4113 431
Size: 557 183 300 1195 432 747 268 1159
Fragments arranged by size:
1195 1159 747 557 432 300 268 183
EcoNI CCTnn'n_nnAGG
Cuts at: 625 625
Size: 4841
EcoO109I rG'GnC_Cy
Cuts at: 1980 1980
Size: 4841
EcoRI G'AATT_C
Cuts at: 1845 1845
Size: 4841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2860 2860
Size: 4841
FspI TGC'GCA
Cuts at: 1465 2622 1465
Size: 1157 3684
HaeII r_GCGC'y
Cuts at: 2275 2523 3545 4524 2275
Size: 248 1022 979 2592
Fragments arranged by size:
2592 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 192 224 301 307 333 339 1339 1371 2683
Size: 32 77 6 26 6 1000 32 1312
Cuts at: 2683 2713 2715 2745 192
Size: 30 2 30 2288
Fragments arranged by size:
2288 1312 1000 77 32 32 30 30
26 6 6 2
HincII GTy'rAC
Cuts at: 1880 1880
Size: 4841
KasI G'GCGC_C
Cuts at: 2271 2519 2271
Size: 248 4593
KpnI G_GTAC'C
Cuts at: 1861 1861
Size: 4841
MluI A'CGCG_T
Cuts at: 4274 4274
Size: 4841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 809 3512 3696 809
Size: 2703 184 1954
Fragments arranged by size:
2703 1954 184
MscI TGG'CCA
Cuts at: 1828 2602 1828
Size: 774 4067
MslI CAynn'nnrTG
Cuts at: 304 1211 1227 1731 1813 2830 2957 304
Size: 907 16 504 82 1017 127 2188
Fragments arranged by size:
2188 1017 907 504 127 82 16
MspA1I CmG'CkG
Cuts at: 213 333 1516 1952 2266 2626 3639 3884 213
Size: 120 1183 436 314 360 1013 245 1170
Fragments arranged by size:
1183 1170 1013 436 360 314 245 120
NaeI GCC'GGC
Cuts at: 3022 3022
Size: 4841
NarI GG'CG_CC
Cuts at: 2272 2520 2272
Size: 248 4593
NcoI C'CATG_G
Cuts at: 107 107
Size: 4841
NdeI CA'TA_TG
Cuts at: 1460 1460
Size: 4841
NgoMIV G'CCGG_C
Cuts at: 3020 3020
Size: 4841
NspI r_CATG'y
Cuts at: 1396 1894 2394 3301 1396
Size: 498 500 907 2936
Fragments arranged by size:
2936 907 500 498
PasI CC'CwG_GG
Cuts at: 1689 1689
Size: 4841
PciI A'CATG_T
Cuts at: 3297 3297
Size: 4841
PmeI GTTT'AAAC
Cuts at: 1840 1840
Size: 4841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 3980
Size: 32 4809
PstI C_TGCA'G
Cuts at: 584 1280 1423 1888 2573 584
Size: 696 143 465 685 2852
Fragments arranged by size:
2852 696 685 465 143
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4841
PvuII CAG'CTG
Cuts at: 213 333 2266 2626 213
Size: 120 1933 360 2428
Fragments arranged by size:
2428 1933 360 120
RsrII CG'GwC_CG
Cuts at: 3036 3036
Size: 4841
SacI G_AGCT'C
Cuts at: 1855 1855
Size: 4841
SalI G'TCGA_C
Cuts at: 1878 1878
Size: 4841
SapI GCTCTTCn'nnn_
Cuts at: 563 2864 3074 563
Size: 2301 210 2330
Fragments arranged by size:
2330 2301 210
SbfI CC_TGCA'GG
Cuts at: 584 1888 584
Size: 1304 3537
ScaI AGT'ACT
Cuts at: 4358 4358
Size: 4841
SfcI C'TryA_G
Cuts at: 33 392 580 789 957 1276 1419 1884 2569
Size: 359 188 209 168 319 143 465 685
Cuts at: 2569 3562 3753 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 465 359 319 209 191
188 168 143
SfoI GGC'GCC
Cuts at: 2273 2521 2273
Size: 248 4593
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4841
SmaI CCC'GGG
Cuts at: 682 1863 682
Size: 1181 3660
SmlI C'TyrA_G
Cuts at: 1541 1547 1571 1996 2911 3403 3665 1541
Size: 6 24 425 915 492 262 2717
Fragments arranged by size:
2717 915 492 425 262 24 6
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4841
SphI G_CATG'C
Cuts at: 1396 1894 2394 1396
Size: 498 500 3843
Fragments arranged by size:
3843 500 498
StuI AGG'CCT
Cuts at: 1165 1594 1165
Size: 429 4412
StyI C'CwwG_G
Cuts at: 107 1397 1975 2329 107
Size: 1290 578 354 2619
Fragments arranged by size:
2619 1290 578 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1253 2424 2557 3016 3225 1253
Size: 1171 133 459 209 2869
Fragments arranged by size:
2869 1171 459 209 133
TatI w'GTAC_w
Cuts at: 1403 4356 1403
Size: 2953 1888
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1649 1681 4358 4390 1649
Size: 32 2677 32 2100
Fragments arranged by size:
2677 2100 32 32
Tth111I GACn'n_nGTC
Cuts at: 2638 2638
Size: 4841
XbaI T'CTAG_A
Cuts at: 1872 1872
Size: 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1696 2336 1696
Size: 640 4201
XmaI C'CCGG_G
Cuts at: 680 1861 680
Size: 1181 3660
XmnI GAAnn'nnTTC
Cuts at: 982 1165 1255 982
Size: 183 90 4568
Fragments arranged by size:
4568 183 90
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PasI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BbsI BbvCI BcgI BfrBI BmtI BsaXI BseRI BsiWI
BsmI BsmBI BssHII BstEII ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI