Restriction map for
pF1KB5966
(Circular) (Six-base) MAPSORT of: pF1KB5966.seq Check: 8534 from: 1 to: 4778
pF1KB5966 4778 bp
With 182 enzymes: SgfI *
January 6, 2012 11:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1213 1213
Size: 4778
AccI GT'mk_AC
Cuts at: 1562 1816 1562
Size: 254 4524
Acc65I G'GTAC_C
Cuts at: 1794 1794
Size: 4778
AclI AA'CG_TT
Cuts at: 1772 4618 1772
Size: 2846 1932
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1019 1045 2603 3035 3782 4050 1019
Size: 26 1558 432 747 268 1747
Fragments arranged by size:
1747 1558 747 432 268 26
AflIII A'CryG_T
Cuts at: 2130 3234 4211 2130
Size: 1104 977 2697
Fragments arranged by size:
2697 1104 977
AgeI A'CCGG_T
Cuts at: 3166 3166
Size: 4778
AleI CACnn'nnGTG
Cuts at: 118 118
Size: 4778
AlwNI CAG_nnn'CTG
Cuts at: 346 1068 2083 3650 346
Size: 722 1015 1567 1474
Fragments arranged by size:
1567 1474 1015 722
ApaI G_GGCC'C
Cuts at: 340 469 340
Size: 129 4649
ApaLI G'TGCA_C
Cuts at: 3548 3548
Size: 4778
ApoI r'AATT_y
Cuts at: 787 1267 1782 4131 4387 787
Size: 480 515 2349 256 1178
Fragments arranged by size:
2349 1178 515 480 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4778
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
AvaI C'yCGr_G
Cuts at: 1798 1798
Size: 4778
BamHI G'GATC_C
Cuts at: 1803 1803
Size: 4778
BanI G'GyrC_C
Cuts at: 330 1794 2208 2456 2491 330
Size: 1464 414 248 35 2617
Fragments arranged by size:
2617 1464 414 248 35
BanII G_rGCy'C
Cuts at: 268 340 469 854 1183 1592 1792 2822 268
Size: 72 129 385 329 409 200 1030 2224
Fragments arranged by size:
2224 1030 409 385 329 200 129 72
BbeI G_GCGC'C
Cuts at: 2212 2460 2212
Size: 248 4530
BbsI GAAGACnn'nnnn_
Cuts at: 222 222
Size: 4778
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1167 1201 90
Size: 34 1043 34 3667
Fragments arranged by size:
3667 1043 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2676 3437 4778 2676
Size: 761 1341 2676
Fragments arranged by size:
2676 1341 761
BclI T'GATC_A
Cuts at: 1286 2298 1286
Size: 1012 3766
BglI GCCn_nnn'nGGC
Cuts at: 479 1363 479
Size: 884 3894
BglII A'GATC_T
Cuts at: 2293 4330 2293
Size: 2037 2741
BlpI GC'TnA_GC
Cuts at: 1472 1890 1472
Size: 418 4360
Bme1580I G_kGCm'C
Cuts at: 340 469 2403 2496 3552 340
Size: 129 1934 93 1056 1566
Fragments arranged by size:
1934 1566 1056 129 93
BmrI ACTGGGnnnn_n'
Cuts at: 472 1990 2071 2406 4550 472
Size: 1518 81 335 2144 700
Fragments arranged by size:
2144 1518 700 335 81
BmtI G_CTAG'C
Cuts at: 865 865
Size: 4778
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 4778
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 321 698 912 1649 154
Size: 167 377 214 737 3283
Fragments arranged by size:
3283 737 377 214 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1606 1954 2833 3325 3623 1606
Size: 348 879 492 298 2761
Fragments arranged by size:
2761 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 1672 4482 1672
Size: 2810 1968
BsaAI yAC'GTr
Cuts at: 219 710 2131 219
Size: 491 1421 2866
Fragments arranged by size:
2866 1421 491
BsaBI GATnn'nnATC
Cuts at: 6 2292 4329 6
Size: 2286 2037 455
Fragments arranged by size:
2286 2037 455
BsaHI Gr'CG_yC
Cuts at: 496 1314 1505 2209 2457 3159 4655 496
Size: 818 191 704 248 702 1496 619
Fragments arranged by size:
1496 818 704 702 619 248 191
BsaWI w'CCGG_w
Cuts at: 1967 2488 3166 3440 3587 3998 1967
Size: 521 678 274 147 411 2747
Fragments arranged by size:
2747 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1622 1652 1622
Size: 30 4748
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1121 1121
Size: 4778
BseYI C'CCAG_C
Cuts at: 482 865 1272 2203 2961 3538 4034 482
Size: 383 407 931 758 577 496 1226
Fragments arranged by size:
1226 931 758 577 496 407 383
BsiEI CG_ry'CG
Cuts at: 104 1305 2320 2366 3574 104
Size: 1201 1015 46 1208 1308
Fragments arranged by size:
1308 1208 1201 1015 46
BsiHKAI G_wGCw'C
Cuts at: 406 1183 1190 1592 1792 2570 2760 3552 406
Size: 777 7 402 200 778 190 792 1632
Fragments arranged by size:
1632 792 778 777 402 200 190 7
BsmI GAATG_Cn'
Cuts at: 999 999
Size: 4778
BsmBI CGTCTCn'nnnn_
Cuts at: 487 487
Size: 4778
Bsp1286I G_dGCh'C
Cuts at: 268 340 406 469 854 1183 1190 1592 1792
Size: 72 66 63 385 329 7 402 200
Cuts at: 1792 2403 2496 2570 2760 2822 3552 268
Size: 611 93 74 190 62 730 1494
Fragments arranged by size:
1494 730 611 402 385 329 200 190
93 74 72 66 63 62 7
BspEI T'CCGG_A
Cuts at: 3998 3998
Size: 4778
BspHI T'CATG_A
Cuts at: 1283 3954 1283
Size: 2671 2107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 354 1213 1828 2042 2344 2725 354
Size: 859 615 214 302 381 2407
Fragments arranged by size:
2407 859 615 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3070 3124 4608 4773 50
Size: 3020 54 1484 165 55
Fragments arranged by size:
3020 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2690 2690
Size: 4778
BsrFI r'CCGG_y
Cuts at: 1565 2776 2957 3166 1565
Size: 1211 181 209 3177
Fragments arranged by size:
3177 1211 209 181
BsrGI T'GTAC_A
Cuts at: 1163 1163
Size: 4778
BssSI C'ACGA_G
Cuts at: 1176 3049 3407 1176
Size: 1873 358 2547
Fragments arranged by size:
2547 1873 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2155 2155
Size: 4778
BstBI TT'CG_AA
Cuts at: 3139 3139
Size: 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 120 219 120
Size: 99 4679
BstYI r'GATC_y
Cuts at: 1 1803 2270 2293 2628 2874 3886 3972 3984
Size: 1802 467 23 335 246 1012 86 12
Cuts at: 3984 4330 1
Size: 346 449
Fragments arranged by size:
1802 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 119 119
Size: 4778
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1008 2728 2909 3072 1008
Size: 1720 181 163 2714
Fragments arranged by size:
2714 1720 181 163
BtsI GCAGTG_nn'
Cuts at: 2021 2021
Size: 4778
DraI TTT'AAA
Cuts at: 1777 1777
Size: 4778
DrdI GACnn_nn'nnGTC
Cuts at: 2484 3342 2484
Size: 858 3920
EaeI y'GGCC_r
Cuts at: 275 428 630 689 2363 2537 2928 2955 4300
Size: 153 202 59 1674 174 391 27 1345
Cuts at: 4300 275
Size: 753
Fragments arranged by size:
1674 1345 753 391 202 174 153 59
27
EagI C'GGCC_G
Cuts at: 2363 2363
Size: 4778
EarI CTCTTCn'nnn_
Cuts at: 1073 2801 3011 1073
Size: 1728 210 2840
Fragments arranged by size:
2840 1728 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 813 2986 3296 3442 4588 813
Size: 2173 310 146 1146 1003
Fragments arranged by size:
2173 1146 1003 310 146
EcoICRI GAG'CTC
Cuts at: 1181 1590 1790 1181
Size: 409 200 4169
Fragments arranged by size:
4169 409 200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 321 698 912 1019 1045 1649 2603 3035
Size: 167 377 214 107 26 604 954 432
Cuts at: 3035 3782 4050 154
Size: 747 268 882
Fragments arranged by size:
954 882 747 604 432 377 268 214
167 107 26
EcoO109I rG'GnC_Cy
Cuts at: 343 466 1197 1917 343
Size: 123 731 720 3204
Fragments arranged by size:
3204 731 720 123
EcoRI G'AATT_C
Cuts at: 1267 1782 1267
Size: 515 4263
EcoRV GAT'ATC
Cuts at: 805 805
Size: 4778
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4778
FspI TGC'GCA
Cuts at: 2559 2559
Size: 4778
HaeII r_GCGC'y
Cuts at: 381 1068 2212 2460 3482 4461 381
Size: 687 1144 248 1022 979 698
Fragments arranged by size:
1144 1022 979 698 687 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2620 2650 2652 2682 2620
Size: 30 2 30 4716
Fragments arranged by size:
4716 30 30 2
HincII GTy'rAC
Cuts at: 1817 1817
Size: 4778
HindIII A'AGCT_T
Cuts at: 1667 1667
Size: 4778
KasI G'GCGC_C
Cuts at: 2208 2456 2208
Size: 248 4530
KpnI G_GTAC'C
Cuts at: 1798 1798
Size: 4778
MluI A'CGCG_T
Cuts at: 4211 4211
Size: 4778
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 533 3449 3633 533
Size: 2916 184 1678
Fragments arranged by size:
2916 1678 184
MscI TGG'CCA
Cuts at: 430 691 2539 430
Size: 261 1848 2669
Fragments arranged by size:
2669 1848 261
MslI CAynn'nnrTG
Cuts at: 118 217 1210 2767 2894 118
Size: 99 993 1557 127 2002
Fragments arranged by size:
2002 1557 993 127 99
MspA1I CmG'CkG
Cuts at: 257 676 1276 1596 1889 2203 2563 3576 3821
Size: 419 600 320 293 314 360 1013 245
Cuts at: 3821 257
Size: 1214
Fragments arranged by size:
1214 1013 600 419 360 320 314 293
245
NaeI GCC'GGC
Cuts at: 2959 2959
Size: 4778
NarI GG'CG_CC
Cuts at: 2209 2457 2209
Size: 248 4530
NgoMIV G'CCGG_C
Cuts at: 2957 2957
Size: 4778
NheI G'CTAG_C
Cuts at: 861 861
Size: 4778
NspI r_CATG'y
Cuts at: 1831 2331 3238 1831
Size: 500 907 3371
Fragments arranged by size:
3371 907 500
PciI A'CATG_T
Cuts at: 3234 3234
Size: 4778
PflMI CCAn_nnn'nTGG
Cuts at: 545 741 545
Size: 196 4582
PfoI T'CCnGG_A
Cuts at: 131 544 131
Size: 413 4365
PmeI GTTT'AAAC
Cuts at: 1777 1777
Size: 4778
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 3917
Size: 32 4746
PpuMI rG'GwC_Cy
Cuts at: 343 343
Size: 4778
PspOMI G'GGCC_C
Cuts at: 336 465 336
Size: 129 4649
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1156 1188 1156
Size: 32 4746
PstI C_TGCA'G
Cuts at: 312 675 1825 2510 312
Size: 363 1150 685 2580
Fragments arranged by size:
2580 1150 685 363
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4778
PvuII CAG'CTG
Cuts at: 676 1276 2203 2563 676
Size: 600 927 360 2891
Fragments arranged by size:
2891 927 600 360
RsrII CG'GwC_CG
Cuts at: 2973 2973
Size: 4778
SacI G_AGCT'C
Cuts at: 1183 1592 1792 1183
Size: 409 200 4169
Fragments arranged by size:
4169 409 200
SalI G'TCGA_C
Cuts at: 1815 1815
Size: 4778
SapI GCTCTTCn'nnn_
Cuts at: 2801 3011 2801
Size: 210 4568
SbfI CC_TGCA'GG
Cuts at: 1825 1825
Size: 4778
ScaI AGT'ACT
Cuts at: 4295 4295
Size: 4778
SexAI A'CCwGG_T
Cuts at: 527 1380 527
Size: 853 3925
SfcI C'TryA_G
Cuts at: 33 308 653 671 1821 2506 3499 3690 33
Size: 275 345 18 1150 685 993 191 1121
Fragments arranged by size:
1150 1121 993 685 345 275 191 18
SfoI GGC'GCC
Cuts at: 2210 2458 2210
Size: 248 4530
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4778
SmaI CCC'GGG
Cuts at: 1800 1800
Size: 4778
SmlI C'TyrA_G
Cuts at: 1585 1933 2848 3340 3602 1585
Size: 348 915 492 262 2761
Fragments arranged by size:
2761 915 492 348 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4778
SphI G_CATG'C
Cuts at: 1831 2331 1831
Size: 500 4278
StyI C'CwwG_G
Cuts at: 735 1324 1912 2266 735
Size: 589 588 354 3247
Fragments arranged by size:
3247 589 588 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 474 2361 2494 2953 3162 474
Size: 1887 133 459 209 2090
Fragments arranged by size:
2090 1887 459 209 133
TatI w'GTAC_w
Cuts at: 1163 4293 1163
Size: 3130 1648
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1501 1533 1622 1654 4295 4327 1501
Size: 32 89 32 2641 32 1952
Fragments arranged by size:
2641 1952 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 127 1120 2575 127
Size: 993 1455 2330
Fragments arranged by size:
2330 1455 993
XbaI T'CTAG_A
Cuts at: 1809 1809
Size: 4778
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 707 2273 707
Size: 1566 3212
XmaI C'CCGG_G
Cuts at: 1798 1798
Size: 4778
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI Bpu10I BsgI BsiWI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PspXI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI