Restriction map for
pF1KB5967
(Circular) (Six-base) MAPSORT of: pF1KB5967.seq Check: 3815 from: 1 to: 4667
pF1KB5967 4667 bp
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 1705 1705
Size: 4667
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 2492 2924 3671 3939 1537
Size: 955 432 747 268 2265
Fragments arranged by size:
2265 955 747 432 268
AflIII A'CryG_T
Cuts at: 1304 2019 3123 4100 1304
Size: 715 1104 977 1871
Fragments arranged by size:
1871 1104 977 715
AgeI A'CCGG_T
Cuts at: 3055 3055
Size: 4667
AleI CACnn'nnGTG
Cuts at: 1348 1348
Size: 4667
AlwNI CAG_nnn'CTG
Cuts at: 1197 1334 1972 3539 1197
Size: 137 638 1567 2325
Fragments arranged by size:
2325 1567 638 137
ApaI G_GGCC'C
Cuts at: 210 1409 210
Size: 1199 3468
ApaLI G'TGCA_C
Cuts at: 517 1621 3437 517
Size: 1104 1816 1747
Fragments arranged by size:
1816 1747 1104
ApoI r'AATT_y
Cuts at: 304 451 627 1064 1671 4020 4276 304
Size: 147 176 437 607 2349 256 695
Fragments arranged by size:
2349 695 607 437 256 176 147
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 190 202 941 1401 1687 190
Size: 12 739 460 286 3170
Fragments arranged by size:
3170 739 460 286 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1059 1092 1059
Size: 33 4634
BamHI G'GATC_C
Cuts at: 1692 1692
Size: 4667
BanI G'GyrC_C
Cuts at: 1201 1546 1638 1683 2097 2345 2380 1201
Size: 345 92 45 414 248 35 3488
Fragments arranged by size:
3488 414 345 248 92 45 35
BanII G_rGCy'C
Cuts at: 210 494 948 1409 1681 2711 210
Size: 284 454 461 272 1030 2166
Fragments arranged by size:
2166 1030 461 454 284 272
BbeI G_GCGC'C
Cuts at: 2101 2349 2101
Size: 248 4419
BciVI GTATCCnnnnn_n'
Cuts at: 2565 3326 4667 2565
Size: 761 1341 2565
Fragments arranged by size:
2565 1341 761
BclI T'GATC_A
Cuts at: 329 2187 329
Size: 1858 2809
BglI GCCn_nnn'nGGC
Cuts at: 541 541
Size: 4667
BglII A'GATC_T
Cuts at: 2182 4219 2182
Size: 2037 2630
BlpI GC'TnA_GC
Cuts at: 488 1779 488
Size: 1291 3376
Bme1580I G_kGCm'C
Cuts at: 210 322 521 1280 1409 1625 2292 2385 3441
Size: 112 199 759 129 216 667 93 1056
Cuts at: 3441 210
Size: 1436
Fragments arranged by size:
1436 1056 759 667 216 199 129 112
93
BmgBI CAC'GTC
Cuts at: 720 720
Size: 4667
BmrI ACTGGGnnnn_n'
Cuts at: 356 1142 1879 1960 2295 4439 356
Size: 786 737 81 335 2144 584
Fragments arranged by size:
2144 786 737 584 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 4667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 4667
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1843 2722 3214 3512 565
Size: 1278 879 492 298 1720
Fragments arranged by size:
1720 1278 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4371 4371
Size: 4667
BsaAI yAC'GTr
Cuts at: 516 1307 2020 516
Size: 791 713 3163
Fragments arranged by size:
3163 791 713
BsaBI GATnn'nnATC
Cuts at: 6 2181 4218 6
Size: 2175 2037 455
Fragments arranged by size:
2175 2037 455
BsaHI Gr'CG_yC
Cuts at: 2098 2346 3048 4544 2098
Size: 248 702 1496 2221
Fragments arranged by size:
2221 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1856 2377 3055 3329 3476 3887 1856
Size: 521 678 274 147 411 2636
Fragments arranged by size:
2636 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 511 541 1429 1459 1468 1498 511
Size: 30 888 30 9 30 3680
Fragments arranged by size:
3680 888 30 30 30 9
BseYI C'CCAG_C
Cuts at: 211 248 1264 1279 1455 2092 2850 3427 3923
Size: 37 1016 15 176 637 758 577 496
Cuts at: 3923 211
Size: 955
Fragments arranged by size:
1016 955 758 637 577 496 176 37
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 823 1320 1372 696
Size: 127 497 52 3991
Fragments arranged by size:
3991 497 127 52
BsiEI CG_ry'CG
Cuts at: 104 2209 2255 3463 104
Size: 2105 46 1208 1308
Fragments arranged by size:
2105 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 521 892 948 1625 1681 2459 2649 3441 521
Size: 371 56 677 56 778 190 792 1747
Fragments arranged by size:
1747 792 778 677 371 190 56 56
BsmI GAATG_Cn'
Cuts at: 860 860
Size: 4667
BsmBI CGTCTCn'nnnn_
Cuts at: 570 570
Size: 4667
Bsp1286I G_dGCh'C
Cuts at: 210 322 494 521 892 948 1280 1409 1625
Size: 112 172 27 371 56 332 129 216
Cuts at: 1625 1681 2292 2385 2459 2649 2711 3441 210
Size: 56 611 93 74 190 62 730 1436
Fragments arranged by size:
1436 730 611 371 332 216 190 172
129 112 93 74 62 56 56 27
BspEI T'CCGG_A
Cuts at: 3887 3887
Size: 4667
BspHI T'CATG_A
Cuts at: 3843 3843
Size: 4667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 179 438 1717 1931 2233 2614 179
Size: 259 1279 214 302 381 2232
Fragments arranged by size:
2232 1279 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 1172 1390 2959 3013 4497 4662 50
Size: 1122 218 1569 54 1484 165 55
Fragments arranged by size:
1569 1484 1122 218 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2579 2579
Size: 4667
BsrFI r'CCGG_y
Cuts at: 2665 2846 3055 2665
Size: 181 209 4277
Fragments arranged by size:
4277 209 181
BssHII G'CGCG_C
Cuts at: 197 197
Size: 4667
BssSI C'ACGA_G
Cuts at: 2938 3296 2938
Size: 358 4309
BstAPI GCAn_nnn'nTGC
Cuts at: 2044 2044
Size: 4667
BstBI TT'CG_AA
Cuts at: 184 3028 184
Size: 2844 1823
BstYI r'GATC_y
Cuts at: 1 1692 2159 2182 2517 2763 3775 3861 3873
Size: 1691 467 23 335 246 1012 86 12
Cuts at: 3873 4219 1
Size: 346 449
Fragments arranged by size:
1691 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 875 1358 1440 1487 107
Size: 768 483 82 47 3287
Fragments arranged by size:
3287 768 483 82 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2617 2798 2961 2617
Size: 181 163 4323
Fragments arranged by size:
4323 181 163
BtsI GCAGTG_nn'
Cuts at: 752 828 1042 1519 1910 752
Size: 76 214 477 391 3509
Fragments arranged by size:
3509 477 391 214 76
DraI TTT'AAA
Cuts at: 1666 1666
Size: 4667
DraIII CAC_nnn'GTG
Cuts at: 315 663 1150 1620 315
Size: 348 487 470 3362
Fragments arranged by size:
3362 487 470 348
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3231 2373
Size: 858 3809
EaeI y'GGCC_r
Cuts at: 110 504 1309 1433 2252 2426 2817 2844 4189
Size: 394 805 124 819 174 391 27 1345
Cuts at: 4189 110
Size: 588
Fragments arranged by size:
1345 819 805 588 394 391 174 124
27
EagI C'GGCC_G
Cuts at: 2252 2252
Size: 4667
EarI CTCTTCn'nnn_
Cuts at: 2690 2900 2690
Size: 210 4457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 366 2875 3185 3331 4477 366
Size: 2509 310 146 1146 556
Fragments arranged by size:
2509 1146 556 310 146
EcoICRI GAG'CTC
Cuts at: 946 1679 946
Size: 733 3934
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1537 2492 2924 3671 3939 172
Size: 1365 955 432 747 268 900
Fragments arranged by size:
1365 955 900 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1209 1209
Size: 4667
EcoO109I rG'GnC_Cy
Cuts at: 206 207 971 1014 1194 1405 1406 1806 206
Size: 1 764 43 180 211 1 400 3067
Fragments arranged by size:
3067 764 400 211 180 43 1 1
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1337 2686 1337
Size: 1349 3318
FspI TGC'GCA
Cuts at: 2448 2448
Size: 4667
HaeII r_GCGC'y
Cuts at: 2101 2349 3371 4350 2101
Size: 248 1022 979 2418
Fragments arranged by size:
2418 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 644 676 2509 2539 2541 2571 644
Size: 32 1833 30 2 30 2740
Fragments arranged by size:
2740 1833 32 30 30 2
HincII GTy'rAC
Cuts at: 1706 1706
Size: 4667
HindIII A'AGCT_T
Cuts at: 1235 1235
Size: 4667
KasI G'GCGC_C
Cuts at: 2097 2345 2097
Size: 248 4419
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 938 3338 3522 938
Size: 2400 184 2083
Fragments arranged by size:
2400 2083 184
MscI TGG'CCA
Cuts at: 1311 1435 2428 1311
Size: 124 993 3550
Fragments arranged by size:
3550 993 124
MslI CAynn'nnrTG
Cuts at: 325 1348 2656 2783 325
Size: 1023 1308 127 2209
Fragments arranged by size:
2209 1308 1023 127
MspA1I CmG'CkG
Cuts at: 1230 1298 1334 1455 1778 2092 2452 3465 3710
Size: 68 36 121 323 314 360 1013 245
Cuts at: 3710 1230
Size: 2187
Fragments arranged by size:
2187 1013 360 323 314 245 121 68
36
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 2098 2346 2098
Size: 248 4419
NcoI C'CATG_G
Cuts at: 107 1358 1440 107
Size: 1251 82 3334
Fragments arranged by size:
3334 1251 82
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 1245 1320 1720 2220 3127 1245
Size: 75 400 500 907 2785
Fragments arranged by size:
2785 907 500 400 75
PciI A'CATG_T
Cuts at: 3123 3123
Size: 4667
PflMI CCAn_nnn'nTGG
Cuts at: 315 1503 315
Size: 1188 3479
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PmlI CAC'GTG
Cuts at: 1307 1307
Size: 4667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1453 1485 3806 3838 1453
Size: 32 2321 32 2282
Fragments arranged by size:
2321 2282 32 32
PpuMI rG'GwC_Cy
Cuts at: 971 1014 971
Size: 43 4624
PspOMI G'GGCC_C
Cuts at: 206 1405 206
Size: 1199 3468
PstI C_TGCA'G
Cuts at: 1538 1714 2399 1538
Size: 176 685 3806
Fragments arranged by size:
3806 685 176
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 1298 1334 1455 2092 2452 1298
Size: 36 121 637 360 3513
Fragments arranged by size:
3513 637 360 121 36
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 948 1681 948
Size: 733 3934
SalI G'TCGA_C
Cuts at: 1704 1704
Size: 4667
SanDI GG'GwC_CC
Cuts at: 971 971
Size: 4667
SapI GCTCTTCn'nnn_
Cuts at: 2690 2900 2690
Size: 210 4457
SbfI CC_TGCA'GG
Cuts at: 1714 1714
Size: 4667
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SfcI C'TryA_G
Cuts at: 33 1209 1326 1534 1710 2395 3388 3579 33
Size: 1176 117 208 176 685 993 191 1121
Fragments arranged by size:
1176 1121 993 685 208 191 176 117
SfoI GGC'GCC
Cuts at: 2099 2347 2099
Size: 248 4419
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 1689 1689
Size: 4667
SmlI C'TyrA_G
Cuts at: 580 941 1822 2737 3229 3491 580
Size: 361 881 915 492 262 1756
Fragments arranged by size:
1756 915 881 492 361 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 1320 1720 2220 1320
Size: 400 500 3767
Fragments arranged by size:
3767 500 400
StyI C'CwwG_G
Cuts at: 107 338 1358 1440 1588 1801 2155 107
Size: 231 1020 82 148 213 354 2619
Fragments arranged by size:
2619 1020 354 231 213 148 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 262 536 1044 1161 1189 2250 2383 2842 3051
Size: 274 508 117 28 1061 133 459 209
Cuts at: 3051 262
Size: 1878
Fragments arranged by size:
1878 1061 508 459 274 209 133 117
28
TatI w'GTAC_w
Cuts at: 404 4182 404
Size: 3778 889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 511 543 1098 1130 1468 1500 4184
Size: 32 222 32 555 32 338 32 2684
Cuts at: 4184 4216 257
Size: 32 708
Fragments arranged by size:
2684 708 555 338 222 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1218 2464 1218
Size: 1246 3421
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 597 988 2162 597
Size: 391 1174 3102
Fragments arranged by size:
3102 1174 391
XhoI C'TCGA_G
Cuts at: 941 941
Size: 4667
XmaI C'CCGG_G
Cuts at: 1687 1687
Size: 4667
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BbsI BbvCI BcgI BfrBI BmtI Bpu10I BseRI
BsiWI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI