Restriction map for
pF1KB5968
(Circular) (Six-base) MAPSORT of: pF1KB5968.seq Check: 1787 from: 1 to: 4781
pF1KB5968 4781 bp
With 182 enzymes: SgfI *
August 9, 2016 17:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 217 231 217
Size: 14 4767
AatII G_ACGT'C
Cuts at: 1376 1376
Size: 4781
AccI GT'mk_AC
Cuts at: 1630 1819 1630
Size: 189 4592
Acc65I G'GTAC_C
Cuts at: 1797 1797
Size: 4781
AclI AA'CG_TT
Cuts at: 4621 4621
Size: 4781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 1475 1727 2606 3038 3785 4053 278
Size: 1197 252 879 432 747 268 1006
Fragments arranged by size:
1197 1006 879 747 432 268 252
AflII C'TTAA_G
Cuts at: 1635 1635
Size: 4781
AflIII A'CryG_T
Cuts at: 472 1569 2133 3237 4214 472
Size: 1097 564 1104 977 1039
Fragments arranged by size:
1104 1097 1039 977 564
AgeI A'CCGG_T
Cuts at: 3169 3169
Size: 4781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 533 1190 533
Size: 657 4124
AlwNI CAG_nnn'CTG
Cuts at: 2086 3653 2086
Size: 1567 3214
ApaI G_GGCC'C
Cuts at: 123 123
Size: 4781
ApaLI G'TGCA_C
Cuts at: 3551 3551
Size: 4781
ApoI r'AATT_y
Cuts at: 637 1420 1550 1747 1785 4134 4390 637
Size: 783 130 197 38 2349 256 1028
Fragments arranged by size:
2349 1028 783 256 197 130 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 4781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
AvaI C'yCGr_G
Cuts at: 623 1801 623
Size: 1178 3603
BamHI G'GATC_C
Cuts at: 1806 1806
Size: 4781
BanI G'GyrC_C
Cuts at: 1797 2211 2459 2494 1797
Size: 414 248 35 4084
Fragments arranged by size:
4084 414 248 35
BanII G_rGCy'C
Cuts at: 123 1091 1795 2825 123
Size: 968 704 1030 2079
Fragments arranged by size:
2079 1030 968 704
BbeI G_GCGC'C
Cuts at: 2215 2463 2215
Size: 248 4533
BbsI GAAGACnn'nnnn_
Cuts at: 175 284 1368 1403 175
Size: 109 1084 35 3553
Fragments arranged by size:
3553 1084 109 35
BciVI GTATCCnnnnn_n'
Cuts at: 295 385 2679 3440 4781 295
Size: 90 2294 761 1341 295
Fragments arranged by size:
2294 1341 761 295 90
BclI T'GATC_A
Cuts at: 1257 2301 1257
Size: 1044 3737
BglII A'GATC_T
Cuts at: 428 2296 4333 428
Size: 1868 2037 876
Fragments arranged by size:
2037 1868 876
BlpI GC'TnA_GC
Cuts at: 743 1893 743
Size: 1150 3631
Bme1580I G_kGCm'C
Cuts at: 123 827 2406 2499 3555 123
Size: 704 1579 93 1056 1349
Fragments arranged by size:
1579 1349 1056 704 93
BmgBI CAC'GTC
Cuts at: 1572 1572
Size: 4781
BmrI ACTGGGnnnn_n'
Cuts at: 1993 2074 2409 4553 1993
Size: 81 335 2144 2221
Fragments arranged by size:
2221 2144 335 81
BmtI G_CTAG'C
Cuts at: 1561 1561
Size: 4781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1105 3148 1105
Size: 2043 2738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 755 1075 354
Size: 401 320 4060
Fragments arranged by size:
4060 401 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1098 1957 2836 3328 3626 1098
Size: 859 879 492 298 2253
Fragments arranged by size:
2253 879 859 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4485 4485
Size: 4781
BsaAI yAC'GTr
Cuts at: 2134 2134
Size: 4781
BsaBI GATnn'nnATC
Cuts at: 6 2295 4332 6
Size: 2289 2037 455
Fragments arranged by size:
2289 2037 455
BsaHI Gr'CG_yC
Cuts at: 320 754 1373 2212 2460 3162 4658 320
Size: 434 619 839 248 702 1496 443
Fragments arranged by size:
1496 839 702 619 443 434 248
BsaWI w'CCGG_w
Cuts at: 695 1970 2491 3169 3443 3590 4001 695
Size: 1275 521 678 274 147 411 1475
Fragments arranged by size:
1475 1275 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1147 1177 1147
Size: 30 4751
BseYI C'CCAG_C
Cuts at: 2206 2964 3541 4037 2206
Size: 758 577 496 2950
Fragments arranged by size:
2950 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2323 2369 3577 104
Size: 2219 46 1208 1308
Fragments arranged by size:
2219 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1091 1795 2573 2763 3555 1091
Size: 704 778 190 792 2317
Fragments arranged by size:
2317 792 778 704 190
BsmI GAATG_Cn'
Cuts at: 405 405
Size: 4781
Bsp1286I G_dGCh'C
Cuts at: 123 827 1091 1795 2406 2499 2573 2763 2825
Size: 704 264 704 611 93 74 190 62
Cuts at: 2825 3555 123
Size: 730 1349
Fragments arranged by size:
1349 730 704 704 611 264 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4001 4001
Size: 4781
BspHI T'CATG_A
Cuts at: 3957 3957
Size: 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 217 231 1075 1270 1831 2045 2347 2728 217
Size: 14 844 195 561 214 302 381 2270
Fragments arranged by size:
2270 844 561 381 302 214 195 14
BsrBI CCG'CTC
Cuts at: 50 3073 3127 4611 4776 50
Size: 3023 54 1484 165 55
Fragments arranged by size:
3023 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 522 1766 2693 522
Size: 1244 927 2610
Fragments arranged by size:
2610 1244 927
BsrFI r'CCGG_y
Cuts at: 2779 2960 3169 2779
Size: 181 209 4391
Fragments arranged by size:
4391 209 181
BssSI C'ACGA_G
Cuts at: 3052 3410 3052
Size: 358 4423
BstAPI GCAn_nnn'nTGC
Cuts at: 2158 2158
Size: 4781
BstBI TT'CG_AA
Cuts at: 186 3142 186
Size: 2956 1825
BstYI r'GATC_y
Cuts at: 1 428 1806 2273 2296 2631 2877 3889 3975
Size: 427 1378 467 23 335 246 1012 86
Cuts at: 3975 3987 4333 1
Size: 12 346 449
Fragments arranged by size:
1378 1012 467 449 427 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 833 1386 833
Size: 553 4228
BtgI C'CryG_G
Cuts at: 160 1013 160
Size: 853 3928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2731 2912 3075 2731
Size: 181 163 4437
Fragments arranged by size:
4437 181 163
BtsI GCAGTG_nn'
Cuts at: 2024 2024
Size: 4781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 414 449 414
Size: 35 4746
DraI TTT'AAA
Cuts at: 1680 1780 1680
Size: 100 4681
DrdI GACnn_nn'nnGTC
Cuts at: 856 2487 3345 856
Size: 1631 858 2292
Fragments arranged by size:
2292 1631 858
EaeI y'GGCC_r
Cuts at: 642 773 2366 2540 2931 2958 4303 642
Size: 131 1593 174 391 27 1345 1120
Fragments arranged by size:
1593 1345 1120 391 174 131 27
EagI C'GGCC_G
Cuts at: 2366 2366
Size: 4781
EarI CTCTTCn'nnn_
Cuts at: 1079 1568 2804 3014 1079
Size: 489 1236 210 2846
Fragments arranged by size:
2846 1236 489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 843 2989 3299 3445 4591 843
Size: 2146 310 146 1146 1033
Fragments arranged by size:
2146 1146 1033 310 146
EcoICRI GAG'CTC
Cuts at: 1089 1793 1089
Size: 704 4077
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 278 354 755 1075 1475 1727 2606 3038 3785
Size: 76 401 320 400 252 879 432 747
Cuts at: 3785 4053 278
Size: 268 1006
Fragments arranged by size:
1006 879 747 432 401 400 320 268
252 76
EcoO109I rG'GnC_Cy
Cuts at: 119 120 1920 119
Size: 1 1800 2980
Fragments arranged by size:
2980 1800 1
EcoRI G'AATT_C
Cuts at: 1785 1785
Size: 4781
EcoRV GAT'ATC
Cuts at: 912 912
Size: 4781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4781
FspI TGC'GCA
Cuts at: 2562 2562
Size: 4781
HaeII r_GCGC'y
Cuts at: 2215 2463 3485 4464 2215
Size: 248 1022 979 2532
Fragments arranged by size:
2532 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 430 462 2623 2653 2655 2685 430
Size: 32 2161 30 2 30 2526
Fragments arranged by size:
2526 2161 32 30 30 2
HincII GTy'rAC
Cuts at: 1820 1820
Size: 4781
KasI G'GCGC_C
Cuts at: 2211 2459 2211
Size: 248 4533
KpnI G_GTAC'C
Cuts at: 1801 1801
Size: 4781
MfeI C'AATT_G
Cuts at: 1168 1168
Size: 4781
MluI A'CGCG_T
Cuts at: 4214 4214
Size: 4781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3452 3636 3452
Size: 184 4597
MscI TGG'CCA
Cuts at: 2542 2542
Size: 4781
MslI CAynn'nnrTG
Cuts at: 461 2770 2897 461
Size: 2309 127 2345
Fragments arranged by size:
2345 2309 127
MspA1I CmG'CkG
Cuts at: 578 750 1152 1892 2206 2566 3579 3824 578
Size: 172 402 740 314 360 1013 245 1535
Fragments arranged by size:
1535 1013 740 402 360 314 245 172
NaeI GCC'GGC
Cuts at: 2962 2962
Size: 4781
NarI GG'CG_CC
Cuts at: 2212 2460 2212
Size: 248 4533
NcoI C'CATG_G
Cuts at: 1013 1013
Size: 4781
NgoMIV G'CCGG_C
Cuts at: 2960 2960
Size: 4781
NheI G'CTAG_C
Cuts at: 1557 1557
Size: 4781
NspI r_CATG'y
Cuts at: 476 1834 2334 3241 476
Size: 1358 500 907 2016
Fragments arranged by size:
2016 1358 907 500
PciI A'CATG_T
Cuts at: 472 3237 472
Size: 2765 2016
PfoI T'CCnGG_A
Cuts at: 331 331
Size: 4781
PmeI GTTT'AAAC
Cuts at: 1780 1780
Size: 4781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1422 1454 3920 3952 1422
Size: 32 2466 32 2251
Fragments arranged by size:
2466 2251 32 32
PspOMI G'GGCC_C
Cuts at: 119 119
Size: 4781
PstI C_TGCA'G
Cuts at: 228 1828 2513 228
Size: 1600 685 2496
Fragments arranged by size:
2496 1600 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4781
PvuII CAG'CTG
Cuts at: 2206 2566 2206
Size: 360 4421
RsrII CG'GwC_CG
Cuts at: 2976 2976
Size: 4781
SacI G_AGCT'C
Cuts at: 1091 1795 1091
Size: 704 4077
SalI G'TCGA_C
Cuts at: 1818 1818
Size: 4781
SapI GCTCTTCn'nnn_
Cuts at: 1079 1568 2804 3014 1079
Size: 489 1236 210 2846
Fragments arranged by size:
2846 1236 489 210
SbfI CC_TGCA'GG
Cuts at: 228 1828 228
Size: 1600 3181
ScaI AGT'ACT
Cuts at: 4298 4298
Size: 4781
SfcI C'TryA_G
Cuts at: 33 224 1576 1824 2509 3502 3693 33
Size: 191 1352 248 685 993 191 1121
Fragments arranged by size:
1352 1121 993 685 248 191 191
SfoI GGC'GCC
Cuts at: 2213 2461 2213
Size: 248 4533
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
SmaI CCC'GGG
Cuts at: 1803 1803
Size: 4781
SmlI C'TyrA_G
Cuts at: 623 1077 1635 1936 2851 3343 3605 623
Size: 454 558 301 915 492 262 1799
Fragments arranged by size:
1799 915 558 492 454 301 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4781
SphI G_CATG'C
Cuts at: 1834 2334 1834
Size: 500 4281
StyI C'CwwG_G
Cuts at: 1013 1915 2269 1013
Size: 902 354 3525
Fragments arranged by size:
3525 902 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 231 2364 2497 2956 3165 231
Size: 2133 133 459 209 1847
Fragments arranged by size:
2133 1847 459 209 133
TatI w'GTAC_w
Cuts at: 381 684 1343 1485 4296 381
Size: 303 659 142 2811 866
Fragments arranged by size:
2811 866 659 303 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1147 1179 4298 4330 1147
Size: 32 3119 32 1598
Fragments arranged by size:
3119 1598 32 32
Tth111I GACn'n_nGTC
Cuts at: 2578 2578
Size: 4781
XbaI T'CTAG_A
Cuts at: 1287 1812 1287
Size: 525 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 730 2276 730
Size: 1546 3235
XhoI C'TCGA_G
Cuts at: 623 623
Size: 4781
XmaI C'CCGG_G
Cuts at: 1801 1801
Size: 4781
XmnI GAAnn'nnTTC
Cuts at: 416 416
Size: 4781
ZraI GAC'GTC
Cuts at: 1374 1374
Size: 4781
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PfoI PmeI PpiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AhdI AleI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BglI Bpu10I BseRI BsgI BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI DraIII EcoNI
FseI FspAI HindIII HpaI NdeI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI