Restriction map for
pF1KB5973
(Circular) (Six-base) MAPSORT of: pF1KB5973.seq Check: 6380 from: 1 to: 4670
pF1KB5973 4670 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 248 260 1708 248
Size: 12 1448 3210
Fragments arranged by size:
3210 1448 12
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 2495 2927 3674 3942 952
Size: 1543 432 747 268 1680
Fragments arranged by size:
1680 1543 747 432 268
AflIII A'CryG_T
Cuts at: 2022 3126 4103 2022
Size: 1104 977 2589
Fragments arranged by size:
2589 1104 977
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AlwNI CAG_nnn'CTG
Cuts at: 463 1418 1975 3542 463
Size: 955 557 1567 1591
Fragments arranged by size:
1591 1567 955 557
ApaI G_GGCC'C
Cuts at: 224 1077 224
Size: 853 3817
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 1674 4023 4279 1674
Size: 2349 256 2065
Fragments arranged by size:
2349 2065 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 1507 1690 1507
Size: 183 4487
BamHI G'GATC_C
Cuts at: 1695 1695
Size: 4670
BanI G'GyrC_C
Cuts at: 240 453 701 1372 1385 1492 1609 1686 2100
Size: 213 248 671 13 107 117 77 414
Cuts at: 2100 2348 2383 240
Size: 248 35 2527
Fragments arranged by size:
2527 671 414 248 248 213 117 107
77 35 13
BanII G_rGCy'C
Cuts at: 224 299 821 832 848 1077 1684 2714 224
Size: 75 522 11 16 229 607 1030 2180
Fragments arranged by size:
2180 1030 607 522 229 75 16 11
BbeI G_GCGC'C
Cuts at: 244 705 1376 1496 1613 2104 2352 244
Size: 461 671 120 117 491 248 2562
Fragments arranged by size:
2562 671 491 461 248 120 117
BbsI GAAGACnn'nnnn_
Cuts at: 1425 1425
Size: 4670
BciVI GTATCCnnnnn_n'
Cuts at: 121 2568 3329 4670 121
Size: 2447 761 1341 121
Fragments arranged by size:
2447 1341 761 121
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BglI GCCn_nnn'nGGC
Cuts at: 232 1318 232
Size: 1086 3584
BglII A'GATC_T
Cuts at: 2185 4222 2185
Size: 2037 2633
BlpI GC'TnA_GC
Cuts at: 1199 1782 1199
Size: 583 4087
Bme1580I G_kGCm'C
Cuts at: 224 1077 1589 2295 2388 3444 224
Size: 853 512 706 93 1056 1450
Fragments arranged by size:
1450 1056 853 706 512 93
BmgBI CAC'GTC
Cuts at: 728 1343 728
Size: 615 4055
BmrI ACTGGGnnnn_n'
Cuts at: 144 1428 1882 1963 2298 4442 144
Size: 1284 454 81 335 2144 372
Fragments arranged by size:
2144 1284 454 372 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 318 3037 318
Size: 2719 1951
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 985 1087 1213 892
Size: 93 102 126 4349
Fragments arranged by size:
4349 126 102 93
Bpu10I CC'TnA_GC
Cuts at: 175 1261 175
Size: 1086 3584
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 691 808 1276 1846 2725 3217 3515 562
Size: 129 117 468 570 879 492 298 1717
Fragments arranged by size:
1717 879 570 492 468 298 129 117
BsaI GGTCTCn'nnnn_
Cuts at: 556 678 4374 556
Size: 122 3696 852
Fragments arranged by size:
3696 852 122
BsaAI yAC'GTr
Cuts at: 182 366 2023 182
Size: 184 1657 2829
Fragments arranged by size:
2829 1657 184
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 241 320 385 702 1180 1373 1493 1595 1610
Size: 79 65 317 478 193 120 102 15
Cuts at: 1610 2101 2349 3051 4547 241
Size: 491 248 702 1496 364
Fragments arranged by size:
1496 702 491 478 364 317 248 193
120 102 79 65 15
BsaWI w'CCGG_w
Cuts at: 193 1019 1859 2380 3058 3332 3479 3890 193
Size: 826 840 521 678 274 147 411 973
Fragments arranged by size:
973 840 826 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 121 151 1378 1408 121
Size: 30 1227 30 3383
Fragments arranged by size:
3383 1227 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 129 796 799 802 814 817 829 874 129
Size: 667 3 3 12 3 12 45 3925
Fragments arranged by size:
3925 667 45 12 12 3 3 3
BseYI C'CCAG_C
Cuts at: 315 1158 1527 1599 2095 2853 3430 3926 315
Size: 843 369 72 496 758 577 496 1059
Fragments arranged by size:
1059 843 758 577 496 496 369 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 1538
Size: 4670
BsiEI CG_ry'CG
Cuts at: 104 308 1103 2212 2258 3466 104
Size: 204 795 1109 46 1208 1308
Fragments arranged by size:
1308 1208 1109 795 204 46
BsiHKAI G_wGCw'C
Cuts at: 181 299 599 832 1684 2462 2652 3444 181
Size: 118 300 233 852 778 190 792 1407
Fragments arranged by size:
1407 852 792 778 300 233 190 118
Bsp1286I G_dGCh'C
Cuts at: 181 224 299 599 821 832 848 1077 1589
Size: 43 75 300 222 11 16 229 512
Cuts at: 1589 1684 2295 2388 2462 2652 2714 3444 181
Size: 95 611 93 74 190 62 730 1407
Fragments arranged by size:
1407 730 611 512 300 229 222 190
95 93 75 74 62 43 16 11
BspEI T'CCGG_A
Cuts at: 193 1019 3890 193
Size: 826 2871 973
Fragments arranged by size:
2871 973 826
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1720 1934 2236 2617 1720
Size: 214 302 381 3773
Fragments arranged by size:
3773 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2962 3016 4500 4665 50
Size: 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2582 2582
Size: 4670
BsrFI r'CCGG_y
Cuts at: 243 269 698 918 1170 1642 2668 2849 3058
Size: 26 429 220 252 472 1026 181 209
Cuts at: 3058 243
Size: 1855
Fragments arranged by size:
1855 1026 472 429 252 220 209 181
26
BssHII G'CGCG_C
Cuts at: 286 694 1013 286
Size: 408 319 3943
Fragments arranged by size:
3943 408 319
BssSI C'ACGA_G
Cuts at: 292 2941 3299 292
Size: 2649 358 1663
Fragments arranged by size:
2649 1663 358
BstAPI GCAn_nnn'nTGC
Cuts at: 185 2047 185
Size: 1862 2808
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstEII G'GTnAC_C
Cuts at: 1401 1401
Size: 4670
BstYI r'GATC_y
Cuts at: 1 433 1695 2162 2185 2520 2766 3778 3864
Size: 432 1262 467 23 335 246 1012 86
Cuts at: 3864 3876 4222 1
Size: 12 346 449
Fragments arranged by size:
1262 1012 467 449 432 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1008 1139 1450 1008
Size: 131 311 4228
Fragments arranged by size:
4228 311 131
BtgI C'CryG_G
Cuts at: 308 524 736 848 1034 1307 308
Size: 216 212 112 186 273 3671
Fragments arranged by size:
3671 273 216 212 186 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2620 2801 2964 2620
Size: 181 163 4326
Fragments arranged by size:
4326 181 163
BtsI GCAGTG_nn'
Cuts at: 1114 1913 1114
Size: 799 3871
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 994 2376 3234 994
Size: 1382 858 2430
Fragments arranged by size:
2430 1382 858
EaeI y'GGCC_r
Cuts at: 161 278 305 641 730 1031 1100 2255 2429
Size: 117 27 336 89 301 69 1155 174
Cuts at: 2429 2820 2847 4192 161
Size: 391 27 1345 639
Fragments arranged by size:
1345 1155 639 391 336 301 174 117
89 69 27 27
EagI C'GGCC_G
Cuts at: 305 1100 2255 305
Size: 795 1155 2720
Fragments arranged by size:
2720 1155 795
EarI CTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 226 356 378 1058 1565 2878 3188 3334 4480
Size: 130 22 680 507 1313 310 146 1146
Cuts at: 4480 226
Size: 416
Fragments arranged by size:
1313 1146 680 507 416 310 146 130
22
EcoICRI GAG'CTC
Cuts at: 297 830 1682 297
Size: 533 852 3285
Fragments arranged by size:
3285 852 533
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 892 952 985 1087 1213 2495 2927 3674 3942
Size: 60 33 102 126 1282 432 747 268
Cuts at: 3942 892
Size: 1620
Fragments arranged by size:
1620 1282 747 432 268 126 102 60
33
EcoNI CCTnn'n_nnAGG
Cuts at: 510 667 709 1636 510
Size: 157 42 927 3544
Fragments arranged by size:
3544 927 157 42
EcoO109I rG'GnC_Cy
Cuts at: 331 447 1062 1073 1303 1809 331
Size: 116 615 11 230 506 3192
Fragments arranged by size:
3192 615 506 230 116 11
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 957 2689 957
Size: 1732 2938
FspI TGC'GCA
Cuts at: 440 750 2451 440
Size: 310 1701 2659
Fragments arranged by size:
2659 1701 310
HaeII r_GCGC'y
Cuts at: 244 316 705 1299 1376 1496 1613 2104 2352
Size: 72 389 594 77 120 117 491 248
Cuts at: 2352 3374 4353 244
Size: 1022 979 561
Fragments arranged by size:
1022 979 594 561 491 389 248 120
117 77 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1264 1296 2512 2542 2544 2574 1264
Size: 32 1216 30 2 30 3360
Fragments arranged by size:
3360 1216 32 30 30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
KasI G'GCGC_C
Cuts at: 240 701 1372 1492 1609 2100 2348 240
Size: 461 671 120 117 491 248 2562
Fragments arranged by size:
2562 671 491 461 248 120 117
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3341 3525 3341
Size: 184 4486
MscI TGG'CCA
Cuts at: 163 280 643 2431 163
Size: 117 363 1788 2402
Fragments arranged by size:
2402 1788 363 117
MslI CAynn'nnrTG
Cuts at: 655 2659 2786 655
Size: 2004 127 2539
Fragments arranged by size:
2539 2004 127
MspA1I CmG'CkG
Cuts at: 310 463 533 738 903 1036 1240 1309 1603
Size: 153 70 205 165 133 204 69 294
Cuts at: 1603 1781 2095 2455 3468 3713 310
Size: 178 314 360 1013 245 1267
Fragments arranged by size:
1267 1013 360 314 294 245 205 204
178 165 153 133 70 69
NaeI GCC'GGC
Cuts at: 271 700 920 1172 1644 2851 271
Size: 429 220 252 472 1207 2090
Fragments arranged by size:
2090 1207 472 429 252 220
NarI GG'CG_CC
Cuts at: 241 702 1373 1493 1610 2101 2349 241
Size: 461 671 120 117 491 248 2562
Fragments arranged by size:
2562 671 491 461 248 120 117
NcoI C'CATG_G
Cuts at: 524 524
Size: 4670
NgoMIV G'CCGG_C
Cuts at: 269 698 918 1170 1642 2849 269
Size: 429 220 252 472 1207 2090
Fragments arranged by size:
2090 1207 472 429 252 220
NspI r_CATG'y
Cuts at: 1290 1723 2223 3130 1290
Size: 433 500 907 2830
Fragments arranged by size:
2830 907 500 433
PciI A'CATG_T
Cuts at: 3126 3126
Size: 4670
PflMI CCAn_nnn'nTGG
Cuts at: 951 951
Size: 4670
PfoI T'CCnGG_A
Cuts at: 1499 1499
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PmlI CAC'GTG
Cuts at: 182 182
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PpuMI rG'GwC_Cy
Cuts at: 331 1062 1303 331
Size: 731 241 3698
Fragments arranged by size:
3698 731 241
PshAI GACnn'nnGTC
Cuts at: 959 959
Size: 4670
PspOMI G'GGCC_C
Cuts at: 220 1073 220
Size: 853 3817
PstI C_TGCA'G
Cuts at: 593 1608 1717 2402 593
Size: 1015 109 685 2861
Fragments arranged by size:
2861 1015 685 109
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 1603 2095 2455 1603
Size: 492 360 3818
Fragments arranged by size:
3818 492 360
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 299 832 1684 299
Size: 533 852 3285
Fragments arranged by size:
3285 852 533
SacII CC_GC'GG
Cuts at: 311 739 1037 1310 311
Size: 428 298 273 3671
Fragments arranged by size:
3671 428 298 273
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SanDI GG'GwC_CC
Cuts at: 331 331
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SexAI A'CCwGG_T
Cuts at: 679 679
Size: 4670
SfcI C'TryA_G
Cuts at: 33 589 1604 1713 2398 3391 3582 33
Size: 556 1015 109 685 993 191 1121
Fragments arranged by size:
1121 1015 993 685 556 191 109
SfoI GGC'GCC
Cuts at: 242 703 1374 1494 1611 2102 2350 242
Size: 461 671 120 117 491 248 2562
Fragments arranged by size:
2562 671 491 461 248 120 117
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SgrAI Cr'CCGG_yG
Cuts at: 698 918 698
Size: 220 4450
SmaI CCC'GGG
Cuts at: 1509 1692 1509
Size: 183 4487
SmlI C'TyrA_G
Cuts at: 577 706 823 1291 1825 2740 3232 3494 577
Size: 129 117 468 534 915 492 262 1753
Fragments arranged by size:
1753 915 534 492 468 262 129 117
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1290 1723 2223 1290
Size: 433 500 3737
Fragments arranged by size:
3737 500 433
StuI AGG'CCT
Cuts at: 583 1006 1190 1474 583
Size: 423 184 284 3779
Fragments arranged by size:
3779 423 284 184
StyI C'CwwG_G
Cuts at: 419 524 620 1299 1487 1637 1804 2158 419
Size: 105 96 679 188 150 167 354 2931
Fragments arranged by size:
2931 679 354 188 167 150 105 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1410 2253 2386 2845 3054 1410
Size: 843 133 459 209 3026
Fragments arranged by size:
3026 843 459 209 133
TatI w'GTAC_w
Cuts at: 4185 4185
Size: 4670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1378 1410 4187 4219 1378
Size: 32 2777 32 1829
Fragments arranged by size:
2777 1829 32 32
Tth111I GACn'n_nGTC
Cuts at: 1429 2467 1429
Size: 1038 3632
XbaI T'CTAG_A
Cuts at: 1701 1701
Size: 4670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 4670
XmaI C'CCGG_G
Cuts at: 1507 1690 1507
Size: 183 4487
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmtI BsiWI
BsmI BsmBI BsrGI BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PsiI PspXI PsrI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI ZraI