(Circular) (Six-base) MAPSORT of: pF1KB5975.seq Check: 779 from: 1 to: 4670 pF1KB5975 4670 bp With 182 enzymes: SgfI * October 7, 2009 14:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 414 414 Size: 4670 AccI GT'mk_AC Cuts at: 677 1708 677 Size: 1031 3639 Acc65I G'GTAC_C Cuts at: 1686 1686 Size: 4670 AclI AA'CG_TT Cuts at: 4510 4510 Size: 4670 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 571 1511 2495 2927 3674 3942 571 Size: 940 984 432 747 268 1299 Fragments arranged by size: 1299 984 940 747 432 268 AflIII A'CryG_T Cuts at: 382 2022 3126 4103 382 Size: 1640 1104 977 949 Fragments arranged by size: 1640 1104 977 949 AgeI A'CCGG_T Cuts at: 3058 3058 Size: 4670 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 859 1082 859 Size: 223 4447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 126 158 126 Size: 32 4638 AlwNI CAG_nnn'CTG Cuts at: 1491 1534 1561 1615 1975 3542 1491 Size: 43 27 54 360 1567 2619 Fragments arranged by size: 2619 1567 360 54 43 27 ApaLI G'TGCA_C Cuts at: 3440 3440 Size: 4670 ApoI r'AATT_y Cuts at: 1342 1674 4023 4279 1342 Size: 332 2349 256 1733 Fragments arranged by size: 2349 1733 332 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4670 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4670 AvaI C'yCGr_G Cuts at: 1690 1690 Size: 4670 AvrII C'CTAG_G Cuts at: 528 528 Size: 4670 BamHI G'GATC_C Cuts at: 1695 1695 Size: 4670 BanI G'GyrC_C Cuts at: 1005 1686 2100 2348 2383 1005 Size: 681 414 248 35 3292 Fragments arranged by size: 3292 681 414 248 35 BanII G_rGCy'C Cuts at: 1127 1498 1684 2714 1127 Size: 371 186 1030 3083 Fragments arranged by size: 3083 1030 371 186 BbeI G_GCGC'C Cuts at: 2104 2352 2104 Size: 248 4422 BbsI GAAGACnn'nnnn_ Cuts at: 1517 1517 Size: 4670 BbvCI CC'TCA_GC Cuts at: 113 113 Size: 4670 BciVI GTATCCnnnnn_n' Cuts at: 2568 3329 4670 2568 Size: 761 1341 2568 Fragments arranged by size: 2568 1341 761 BclI T'GATC_A Cuts at: 2190 2190 Size: 4670 BfrBI ATG'CAT Cuts at: 1069 1069 Size: 4670 BglI GCCn_nnn'nGGC Cuts at: 262 826 262 Size: 564 4106 BglII A'GATC_T Cuts at: 433 2185 4222 433 Size: 1752 2037 881 Fragments arranged by size: 2037 1752 881 BlpI GC'TnA_GC Cuts at: 1782 1782 Size: 4670 Bme1580I G_kGCm'C Cuts at: 2295 2388 3444 2295 Size: 93 1056 3521 Fragments arranged by size: 3521 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1569 1882 1963 2298 4442 1569 Size: 313 81 335 2144 1797 Fragments arranged by size: 2144 1797 335 313 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1405 3037 1405 Size: 1632 3038 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 170 667 1375 1583 1624 170 Size: 497 708 208 41 3216 Fragments arranged by size: 3216 708 497 208 41 Bpu10I CC'TnA_GC Cuts at: 113 113 Size: 4670 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 403 1306 1846 2725 3217 3515 403 Size: 903 540 879 492 298 1558 Fragments arranged by size: 1558 903 879 540 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4374 4374 Size: 4670 BsaAI yAC'GTr Cuts at: 2023 2023 Size: 4670 BsaBI GATnn'nnATC Cuts at: 6 2184 4221 6 Size: 2178 2037 455 Fragments arranged by size: 2178 2037 455 BsaHI Gr'CG_yC Cuts at: 2101 2349 3051 4547 2101 Size: 248 702 1496 2224 Fragments arranged by size: 2224 1496 702 248 BsaWI w'CCGG_w Cuts at: 239 1859 2380 3058 3332 3479 3890 239 Size: 1620 521 678 274 147 411 1019 Fragments arranged by size: 1620 1019 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 130 130 Size: 4670 BseYI C'CCAG_C Cuts at: 627 2095 2853 3430 3926 627 Size: 1468 758 577 496 1371 Fragments arranged by size: 1468 1371 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 407 407 Size: 4670 BsiEI CG_ry'CG Cuts at: 104 2212 2258 3466 104 Size: 2108 46 1208 1308 Fragments arranged by size: 2108 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1127 1684 2462 2652 3444 1127 Size: 557 778 190 792 2353 Fragments arranged by size: 2353 792 778 557 190 Bsp1286I G_dGCh'C Cuts at: 1127 1498 1684 2295 2388 2462 2652 2714 3444 Size: 371 186 611 93 74 190 62 730 Cuts at: 3444 1127 Size: 2353 Fragments arranged by size: 2353 730 611 371 190 186 93 74 62 BspEI T'CCGG_A Cuts at: 239 3890 239 Size: 3651 1019 BspHI T'CATG_A Cuts at: 3846 3846 Size: 4670 BspMI ACCTGCnnnn'nnnn_ Cuts at: 414 1479 1720 1934 2236 2617 414 Size: 1065 241 214 302 381 2467 Fragments arranged by size: 2467 1065 381 302 241 214 BsrBI CCG'CTC Cuts at: 50 2962 3016 4500 4665 50 Size: 2912 54 1484 165 55 Fragments arranged by size: 2912 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 792 1526 2582 792 Size: 734 1056 2880 Fragments arranged by size: 2880 1056 734 BsrFI r'CCGG_y Cuts at: 2668 2849 3058 2668 Size: 181 209 4280 Fragments arranged by size: 4280 209 181 BsrGI T'GTAC_A Cuts at: 1253 1253 Size: 4670 BssSI C'ACGA_G Cuts at: 2941 3299 2941 Size: 358 4312 BstAPI GCAn_nnn'nTGC Cuts at: 2047 2047 Size: 4670 BstBI TT'CG_AA Cuts at: 3031 3031 Size: 4670 BstXI CCAn_nnnn'nTGG Cuts at: 1002 1002 Size: 4670 BstYI r'GATC_y Cuts at: 1 433 635 1431 1695 2162 2185 2520 2766 Size: 432 202 796 264 467 23 335 246 Cuts at: 2766 3778 3864 3876 4222 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 796 467 449 432 346 335 264 246 202 86 23 12 Bsu36I CC'TnA_GG Cuts at: 1554 1554 Size: 4670 BtgI C'CryG_G Cuts at: 107 852 107 Size: 745 3925 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2620 2801 2964 2620 Size: 181 163 4326 Fragments arranged by size: 4326 181 163 BtsI GCAGTG_nn' Cuts at: 695 1148 1913 695 Size: 453 765 3452 Fragments arranged by size: 3452 765 453 DraI TTT'AAA Cuts at: 1669 1669 Size: 4670 DrdI GACnn_nn'nnGTC Cuts at: 2376 3234 2376 Size: 858 3812 EaeI y'GGCC_r Cuts at: 428 971 1094 2255 2429 2820 2847 4192 428 Size: 543 123 1161 174 391 27 1345 906 Fragments arranged by size: 1345 1161 906 543 391 174 123 27 EagI C'GGCC_G Cuts at: 2255 2255 Size: 4670 EarI CTCTTCn'nnn_ Cuts at: 379 1601 2693 2903 379 Size: 1222 1092 210 2146 Fragments arranged by size: 2146 1222 1092 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2878 3188 3334 4480 2878 Size: 310 146 1146 3068 Fragments arranged by size: 3068 1146 310 146 EcoICRI GAG'CTC Cuts at: 1125 1682 1125 Size: 557 4113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 170 571 667 1375 1511 1583 1624 2495 2927 Size: 401 96 708 136 72 41 871 432 Cuts at: 2927 3674 3942 170 Size: 747 268 898 Fragments arranged by size: 898 871 747 708 432 401 268 136 96 72 41 EcoNI CCTnn'n_nnAGG Cuts at: 421 1321 421 Size: 900 3770 EcoO109I rG'GnC_Cy Cuts at: 1809 1809 Size: 4670 EcoRI G'AATT_C Cuts at: 1674 1674 Size: 4670 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 298 657 840 2689 298 Size: 359 183 1849 2279 Fragments arranged by size: 2279 1849 359 183 FspI TGC'GCA Cuts at: 2451 2451 Size: 4670 HaeII r_GCGC'y Cuts at: 2104 2352 3374 4353 2104 Size: 248 1022 979 2421 Fragments arranged by size: 2421 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 120 152 1318 1350 1423 1455 2512 2542 2544 Size: 32 1166 32 73 32 1057 30 2 Cuts at: 2544 2574 120 Size: 30 2216 Fragments arranged by size: 2216 1166 1057 73 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1709 1709 Size: 4670 KasI G'GCGC_C Cuts at: 2100 2348 2100 Size: 248 4422 KpnI G_GTAC'C Cuts at: 1690 1690 Size: 4670 MluI A'CGCG_T Cuts at: 4103 4103 Size: 4670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1657 3341 3525 1657 Size: 1684 184 2802 Fragments arranged by size: 2802 1684 184 MscI TGG'CCA Cuts at: 430 2431 430 Size: 2001 2669 MslI CAynn'nnrTG Cuts at: 937 2659 2786 937 Size: 1722 127 2821 Fragments arranged by size: 2821 1722 127 MspA1I CmG'CkG Cuts at: 980 1534 1781 2095 2455 3468 3713 980 Size: 554 247 314 360 1013 245 1937 Fragments arranged by size: 1937 1013 554 360 314 247 245 NaeI GCC'GGC Cuts at: 2851 2851 Size: 4670 NarI GG'CG_CC Cuts at: 2101 2349 2101 Size: 248 4422 NcoI C'CATG_G Cuts at: 107 107 Size: 4670 NgoMIV G'CCGG_C Cuts at: 2849 2849 Size: 4670 NruI TCG'CGA Cuts at: 127 127 Size: 4670 NsiI A_TGCA'T Cuts at: 1071 1071 Size: 4670 NspI r_CATG'y Cuts at: 386 1134 1723 2223 3130 386 Size: 748 589 500 907 1926 Fragments arranged by size: 1926 907 748 589 500 PciI A'CATG_T Cuts at: 382 3126 382 Size: 2744 1926 PflMI CCAn_nnn'nTGG Cuts at: 645 645 Size: 4670 PmeI GTTT'AAAC Cuts at: 1669 1669 Size: 4670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 3809 Size: 32 4638 PstI C_TGCA'G Cuts at: 1717 2402 1717 Size: 685 3985 PvuI CG_AT'CG Cuts at: 104 104 Size: 4670 PvuII CAG'CTG Cuts at: 980 1534 2095 2455 980 Size: 554 561 360 3195 Fragments arranged by size: 3195 561 554 360 RsrII CG'GwC_CG Cuts at: 2865 2865 Size: 4670 SacI G_AGCT'C Cuts at: 1127 1684 1127 Size: 557 4113 SalI G'TCGA_C Cuts at: 1707 1707 Size: 4670 SapI GCTCTTCn'nnn_ Cuts at: 2693 2903 2693 Size: 210 4460 SbfI CC_TGCA'GG Cuts at: 1717 1717 Size: 4670 ScaI AGT'ACT Cuts at: 4187 4187 Size: 4670 SfcI C'TryA_G Cuts at: 33 1713 2398 3391 3582 33 Size: 1680 685 993 191 1121 Fragments arranged by size: 1680 1121 993 685 191 SfoI GGC'GCC Cuts at: 2102 2350 2102 Size: 248 4422 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4670 SmaI CCC'GGG Cuts at: 1692 1692 Size: 4670 SmlI C'TyrA_G Cuts at: 418 1321 1825 2740 3232 3494 418 Size: 903 504 915 492 262 1594 Fragments arranged by size: 1594 915 903 504 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4670 SphI G_CATG'C Cuts at: 1723 2223 1723 Size: 500 4170 StyI C'CwwG_G Cuts at: 107 528 1804 2158 107 Size: 421 1276 354 2619 Fragments arranged by size: 2619 1276 421 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2253 2386 2845 3054 2253 Size: 133 459 209 3869 Fragments arranged by size: 3869 459 209 133 TatI w'GTAC_w Cuts at: 1253 4185 1253 Size: 2932 1738 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4187 4219 4187 Size: 32 4638 Tth111I GACn'n_nGTC Cuts at: 2467 2467 Size: 4670 XbaI T'CTAG_A Cuts at: 1701 1701 Size: 4670 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 4670 XmaI C'CCGG_G Cuts at: 1690 1690 Size: 4670 XmnI GAAnn'nnTTC Cuts at: 281 556 281 Size: 275 4395 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI NsiI NspI PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI ApaI AscI BaeI BcgI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI