Restriction map for pF1KB5975
 (Circular) (Six-base) MAPSORT of: pF1KB5975.seq  Check: 779  from: 1  to: 4670



pF1KB5975  4670 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    414     414 
   Size:       4670
 
AccI GT'mk_AC

Cuts at:    677    1708     677 
   Size:       1031    3639
 
Acc65I G'GTAC_C

Cuts at:   1686    1686 
   Size:       4670
 
AclI AA'CG_TT

Cuts at:   4510    4510 
   Size:       4670
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    571    1511    2495    2927    3674    3942     571 
   Size:        940     984     432     747     268    1299

  Fragments arranged by size: 

                1299     984     940     747     432     268
 
AflIII A'CryG_T

Cuts at:    382    2022    3126    4103     382 
   Size:       1640    1104     977     949

  Fragments arranged by size: 

                1640    1104     977     949
 
AgeI A'CCGG_T

Cuts at:   3058    3058 
   Size:       4670
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    859    1082     859 
   Size:        223    4447
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    126     158     126 
   Size:         32    4638
 
AlwNI CAG_nnn'CTG

Cuts at:   1491    1534    1561    1615    1975    3542    1491 
   Size:         43      27      54     360    1567    2619

  Fragments arranged by size: 

                2619    1567     360      54      43      27
 
ApaLI G'TGCA_C

Cuts at:   3440    3440 
   Size:       4670
 
ApoI r'AATT_y

Cuts at:   1342    1674    4023    4279    1342 
   Size:        332    2349     256    1733

  Fragments arranged by size: 

                2349    1733     332     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4670
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4670
 
AvaI C'yCGr_G

Cuts at:   1690    1690 
   Size:       4670
 
AvrII C'CTAG_G

Cuts at:    528     528 
   Size:       4670
 
BamHI G'GATC_C

Cuts at:   1695    1695 
   Size:       4670
 
BanI G'GyrC_C

Cuts at:   1005    1686    2100    2348    2383    1005 
   Size:        681     414     248      35    3292

  Fragments arranged by size: 

                3292     681     414     248      35
 
BanII G_rGCy'C

Cuts at:   1127    1498    1684    2714    1127 
   Size:        371     186    1030    3083

  Fragments arranged by size: 

                3083    1030     371     186
 
BbeI G_GCGC'C

Cuts at:   2104    2352    2104 
   Size:        248    4422
 
BbsI GAAGACnn'nnnn_

Cuts at:   1517    1517 
   Size:       4670
 
BbvCI CC'TCA_GC

Cuts at:    113     113 
   Size:       4670
 
BciVI GTATCCnnnnn_n'

Cuts at:   2568    3329    4670    2568 
   Size:        761    1341    2568

  Fragments arranged by size: 

                2568    1341     761
 
BclI T'GATC_A

Cuts at:   2190    2190 
   Size:       4670
 
BfrBI ATG'CAT

Cuts at:   1069    1069 
   Size:       4670
 
BglI GCCn_nnn'nGGC

Cuts at:    262     826     262 
   Size:        564    4106
 
BglII A'GATC_T

Cuts at:    433    2185    4222     433 
   Size:       1752    2037     881

  Fragments arranged by size: 

                2037    1752     881
 
BlpI GC'TnA_GC

Cuts at:   1782    1782 
   Size:       4670
 
Bme1580I G_kGCm'C

Cuts at:   2295    2388    3444    2295 
   Size:         93    1056    3521

  Fragments arranged by size: 

                3521    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1569    1882    1963    2298    4442    1569 
   Size:        313      81     335    2144    1797

  Fragments arranged by size: 

                2144    1797     335     313      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1405    3037    1405 
   Size:       1632    3038
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    170     667    1375    1583    1624     170 
   Size:        497     708     208      41    3216

  Fragments arranged by size: 

                3216     708     497     208      41
 
Bpu10I CC'TnA_GC

Cuts at:    113     113 
   Size:       4670
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    403    1306    1846    2725    3217    3515     403 
   Size:        903     540     879     492     298    1558

  Fragments arranged by size: 

                1558     903     879     540     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4374    4374 
   Size:       4670
 
BsaAI yAC'GTr

Cuts at:   2023    2023 
   Size:       4670
 
BsaBI GATnn'nnATC

Cuts at:      6    2184    4221       6 
   Size:       2178    2037     455

  Fragments arranged by size: 

                2178    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   2101    2349    3051    4547    2101 
   Size:        248     702    1496    2224

  Fragments arranged by size: 

                2224    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    239    1859    2380    3058    3332    3479    3890     239 
   Size:       1620     521     678     274     147     411    1019

  Fragments arranged by size: 

                1620    1019     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    130     130 
   Size:       4670
 
BseYI C'CCAG_C

Cuts at:    627    2095    2853    3430    3926     627 
   Size:       1468     758     577     496    1371

  Fragments arranged by size: 

                1468    1371     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    407     407 
   Size:       4670
 
BsiEI CG_ry'CG

Cuts at:    104    2212    2258    3466     104 
   Size:       2108      46    1208    1308

  Fragments arranged by size: 

                2108    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1127    1684    2462    2652    3444    1127 
   Size:        557     778     190     792    2353

  Fragments arranged by size: 

                2353     792     778     557     190
 
Bsp1286I G_dGCh'C

Cuts at:   1127    1498    1684    2295    2388    2462    2652    2714    3444 
   Size:        371     186     611      93      74     190      62     730

Cuts at:   3444    1127 
   Size:       2353

  Fragments arranged by size: 

                2353     730     611     371     190     186      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:    239    3890     239 
   Size:       3651    1019
 
BspHI T'CATG_A

Cuts at:   3846    3846 
   Size:       4670
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    414    1479    1720    1934    2236    2617     414 
   Size:       1065     241     214     302     381    2467

  Fragments arranged by size: 

                2467    1065     381     302     241     214
 
BsrBI CCG'CTC

Cuts at:     50    2962    3016    4500    4665      50 
   Size:       2912      54    1484     165      55

  Fragments arranged by size: 

                2912    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    792    1526    2582     792 
   Size:        734    1056    2880

  Fragments arranged by size: 

                2880    1056     734
 
BsrFI r'CCGG_y

Cuts at:   2668    2849    3058    2668 
   Size:        181     209    4280

  Fragments arranged by size: 

                4280     209     181
 
BsrGI T'GTAC_A

Cuts at:   1253    1253 
   Size:       4670
 
BssSI C'ACGA_G

Cuts at:   2941    3299    2941 
   Size:        358    4312
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2047    2047 
   Size:       4670
 
BstBI TT'CG_AA

Cuts at:   3031    3031 
   Size:       4670
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1002    1002 
   Size:       4670
 
BstYI r'GATC_y

Cuts at:      1     433     635    1431    1695    2162    2185    2520    2766 
   Size:        432     202     796     264     467      23     335     246

Cuts at:   2766    3778    3864    3876    4222       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     796     467     449     432     346     335     264
                 246     202      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1554    1554 
   Size:       4670
 
BtgI C'CryG_G

Cuts at:    107     852     107 
   Size:        745    3925
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2620    2801    2964    2620 
   Size:        181     163    4326

  Fragments arranged by size: 

                4326     181     163
 
BtsI GCAGTG_nn'

Cuts at:    695    1148    1913     695 
   Size:        453     765    3452

  Fragments arranged by size: 

                3452     765     453
 
DraI TTT'AAA

Cuts at:   1669    1669 
   Size:       4670
 
DrdI GACnn_nn'nnGTC

Cuts at:   2376    3234    2376 
   Size:        858    3812
 
EaeI y'GGCC_r

Cuts at:    428     971    1094    2255    2429    2820    2847    4192     428 
   Size:        543     123    1161     174     391      27    1345     906

  Fragments arranged by size: 

                1345    1161     906     543     391     174     123      27
            
 
EagI C'GGCC_G

Cuts at:   2255    2255 
   Size:       4670
 
EarI CTCTTCn'nnn_

Cuts at:    379    1601    2693    2903     379 
   Size:       1222    1092     210    2146

  Fragments arranged by size: 

                2146    1222    1092     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2878    3188    3334    4480    2878 
   Size:        310     146    1146    3068

  Fragments arranged by size: 

                3068    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1125    1682    1125 
   Size:        557    4113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    170     571     667    1375    1511    1583    1624    2495    2927 
   Size:        401      96     708     136      72      41     871     432

Cuts at:   2927    3674    3942     170 
   Size:        747     268     898

  Fragments arranged by size: 

                 898     871     747     708     432     401     268     136
                  96      72      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:    421    1321     421 
   Size:        900    3770
 
EcoO109I rG'GnC_Cy

Cuts at:   1809    1809 
   Size:       4670
 
EcoRI G'AATT_C

Cuts at:   1674    1674 
   Size:       4670
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    298     657     840    2689     298 
   Size:        359     183    1849    2279

  Fragments arranged by size: 

                2279    1849     359     183
 
FspI TGC'GCA

Cuts at:   2451    2451 
   Size:       4670
 
HaeII r_GCGC'y

Cuts at:   2104    2352    3374    4353    2104 
   Size:        248    1022     979    2421

  Fragments arranged by size: 

                2421    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    120     152    1318    1350    1423    1455    2512    2542    2544 
   Size:         32    1166      32      73      32    1057      30       2

Cuts at:   2544    2574     120 
   Size:         30    2216

  Fragments arranged by size: 

                2216    1166    1057      73      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1709    1709 
   Size:       4670
 
KasI G'GCGC_C

Cuts at:   2100    2348    2100 
   Size:        248    4422
 
KpnI G_GTAC'C

Cuts at:   1690    1690 
   Size:       4670
 
MluI A'CGCG_T

Cuts at:   4103    4103 
   Size:       4670
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1657    3341    3525    1657 
   Size:       1684     184    2802

  Fragments arranged by size: 

                2802    1684     184
 
MscI TGG'CCA

Cuts at:    430    2431     430 
   Size:       2001    2669
 
MslI CAynn'nnrTG

Cuts at:    937    2659    2786     937 
   Size:       1722     127    2821

  Fragments arranged by size: 

                2821    1722     127
 
MspA1I CmG'CkG

Cuts at:    980    1534    1781    2095    2455    3468    3713     980 
   Size:        554     247     314     360    1013     245    1937

  Fragments arranged by size: 

                1937    1013     554     360     314     247     245
 
NaeI GCC'GGC

Cuts at:   2851    2851 
   Size:       4670
 
NarI GG'CG_CC

Cuts at:   2101    2349    2101 
   Size:        248    4422
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4670
 
NgoMIV G'CCGG_C

Cuts at:   2849    2849 
   Size:       4670
 
NruI TCG'CGA

Cuts at:    127     127 
   Size:       4670
 
NsiI A_TGCA'T

Cuts at:   1071    1071 
   Size:       4670
 
NspI r_CATG'y

Cuts at:    386    1134    1723    2223    3130     386 
   Size:        748     589     500     907    1926

  Fragments arranged by size: 

                1926     907     748     589     500
 
PciI A'CATG_T

Cuts at:    382    3126     382 
   Size:       2744    1926
 
PflMI CCAn_nnn'nTGG

Cuts at:    645     645 
   Size:       4670
 
PmeI GTTT'AAAC

Cuts at:   1669    1669 
   Size:       4670
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3809    3841    3809 
   Size:         32    4638
 
PstI C_TGCA'G

Cuts at:   1717    2402    1717 
   Size:        685    3985
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4670
 
PvuII CAG'CTG

Cuts at:    980    1534    2095    2455     980 
   Size:        554     561     360    3195

  Fragments arranged by size: 

                3195     561     554     360
 
RsrII CG'GwC_CG

Cuts at:   2865    2865 
   Size:       4670
 
SacI G_AGCT'C

Cuts at:   1127    1684    1127 
   Size:        557    4113
 
SalI G'TCGA_C

Cuts at:   1707    1707 
   Size:       4670
 
SapI GCTCTTCn'nnn_

Cuts at:   2693    2903    2693 
   Size:        210    4460
 
SbfI CC_TGCA'GG

Cuts at:   1717    1717 
   Size:       4670
 
ScaI AGT'ACT

Cuts at:   4187    4187 
   Size:       4670
 
SfcI C'TryA_G

Cuts at:     33    1713    2398    3391    3582      33 
   Size:       1680     685     993     191    1121

  Fragments arranged by size: 

                1680    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   2102    2350    2102 
   Size:        248    4422
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4670
 
SmaI CCC'GGG

Cuts at:   1692    1692 
   Size:       4670
 
SmlI C'TyrA_G

Cuts at:    418    1321    1825    2740    3232    3494     418 
   Size:        903     504     915     492     262    1594

  Fragments arranged by size: 

                1594     915     903     504     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4670
 
SphI G_CATG'C

Cuts at:   1723    2223    1723 
   Size:        500    4170
 
StyI C'CwwG_G

Cuts at:    107     528    1804    2158     107 
   Size:        421    1276     354    2619

  Fragments arranged by size: 

                2619    1276     421     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2253    2386    2845    3054    2253 
   Size:        133     459     209    3869

  Fragments arranged by size: 

                3869     459     209     133
 
TatI w'GTAC_w

Cuts at:   1253    4185    1253 
   Size:       2932    1738
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4187    4219    4187 
   Size:         32    4638
 
Tth111I GACn'n_nGTC

Cuts at:   2467    2467 
   Size:       4670
 
XbaI T'CTAG_A

Cuts at:   1701    1701 
   Size:       4670
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2165    2165 
   Size:       4670
 
XmaI C'CCGG_G

Cuts at:   1690    1690 
   Size:       4670
 
XmnI GAAnn'nnTTC

Cuts at:    281     556     281 
   Size:        275    4395
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AlfI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NruI     NsiI     NspI     PciI    PflMI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     ApaI     AscI     BaeI
     BcgI    BmgBI     BmtI    BsaXI    BsiWI     BsmI    BsmBI   BssHII
   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     PacI     PasI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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