Restriction map for
pF1KB5975
(Circular) (Six-base) MAPSORT of: pF1KB5975.seq Check: 779 from: 1 to: 4670
pF1KB5975 4670 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 414 414
Size: 4670
AccI GT'mk_AC
Cuts at: 677 1708 677
Size: 1031 3639
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1511 2495 2927 3674 3942 571
Size: 940 984 432 747 268 1299
Fragments arranged by size:
1299 984 940 747 432 268
AflIII A'CryG_T
Cuts at: 382 2022 3126 4103 382
Size: 1640 1104 977 949
Fragments arranged by size:
1640 1104 977 949
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 859 1082 859
Size: 223 4447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 126 158 126
Size: 32 4638
AlwNI CAG_nnn'CTG
Cuts at: 1491 1534 1561 1615 1975 3542 1491
Size: 43 27 54 360 1567 2619
Fragments arranged by size:
2619 1567 360 54 43 27
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 1342 1674 4023 4279 1342
Size: 332 2349 256 1733
Fragments arranged by size:
2349 1733 332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 1690 1690
Size: 4670
AvrII C'CTAG_G
Cuts at: 528 528
Size: 4670
BamHI G'GATC_C
Cuts at: 1695 1695
Size: 4670
BanI G'GyrC_C
Cuts at: 1005 1686 2100 2348 2383 1005
Size: 681 414 248 35 3292
Fragments arranged by size:
3292 681 414 248 35
BanII G_rGCy'C
Cuts at: 1127 1498 1684 2714 1127
Size: 371 186 1030 3083
Fragments arranged by size:
3083 1030 371 186
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BbsI GAAGACnn'nnnn_
Cuts at: 1517 1517
Size: 4670
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 4670
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BfrBI ATG'CAT
Cuts at: 1069 1069
Size: 4670
BglI GCCn_nnn'nGGC
Cuts at: 262 826 262
Size: 564 4106
BglII A'GATC_T
Cuts at: 433 2185 4222 433
Size: 1752 2037 881
Fragments arranged by size:
2037 1752 881
BlpI GC'TnA_GC
Cuts at: 1782 1782
Size: 4670
Bme1580I G_kGCm'C
Cuts at: 2295 2388 3444 2295
Size: 93 1056 3521
Fragments arranged by size:
3521 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1569 1882 1963 2298 4442 1569
Size: 313 81 335 2144 1797
Fragments arranged by size:
2144 1797 335 313 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1405 3037 1405
Size: 1632 3038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 667 1375 1583 1624 170
Size: 497 708 208 41 3216
Fragments arranged by size:
3216 708 497 208 41
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 4670
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1306 1846 2725 3217 3515 403
Size: 903 540 879 492 298 1558
Fragments arranged by size:
1558 903 879 540 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4374 4374
Size: 4670
BsaAI yAC'GTr
Cuts at: 2023 2023
Size: 4670
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 2101 2349 3051 4547 2101
Size: 248 702 1496 2224
Fragments arranged by size:
2224 1496 702 248
BsaWI w'CCGG_w
Cuts at: 239 1859 2380 3058 3332 3479 3890 239
Size: 1620 521 678 274 147 411 1019
Fragments arranged by size:
1620 1019 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 130
Size: 4670
BseYI C'CCAG_C
Cuts at: 627 2095 2853 3430 3926 627
Size: 1468 758 577 496 1371
Fragments arranged by size:
1468 1371 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 407
Size: 4670
BsiEI CG_ry'CG
Cuts at: 104 2212 2258 3466 104
Size: 2108 46 1208 1308
Fragments arranged by size:
2108 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1127 1684 2462 2652 3444 1127
Size: 557 778 190 792 2353
Fragments arranged by size:
2353 792 778 557 190
Bsp1286I G_dGCh'C
Cuts at: 1127 1498 1684 2295 2388 2462 2652 2714 3444
Size: 371 186 611 93 74 190 62 730
Cuts at: 3444 1127
Size: 2353
Fragments arranged by size:
2353 730 611 371 190 186 93 74
62
BspEI T'CCGG_A
Cuts at: 239 3890 239
Size: 3651 1019
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 414 1479 1720 1934 2236 2617 414
Size: 1065 241 214 302 381 2467
Fragments arranged by size:
2467 1065 381 302 241 214
BsrBI CCG'CTC
Cuts at: 50 2962 3016 4500 4665 50
Size: 2912 54 1484 165 55
Fragments arranged by size:
2912 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 792 1526 2582 792
Size: 734 1056 2880
Fragments arranged by size:
2880 1056 734
BsrFI r'CCGG_y
Cuts at: 2668 2849 3058 2668
Size: 181 209 4280
Fragments arranged by size:
4280 209 181
BsrGI T'GTAC_A
Cuts at: 1253 1253
Size: 4670
BssSI C'ACGA_G
Cuts at: 2941 3299 2941
Size: 358 4312
BstAPI GCAn_nnn'nTGC
Cuts at: 2047 2047
Size: 4670
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 1002 1002
Size: 4670
BstYI r'GATC_y
Cuts at: 1 433 635 1431 1695 2162 2185 2520 2766
Size: 432 202 796 264 467 23 335 246
Cuts at: 2766 3778 3864 3876 4222 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 796 467 449 432 346 335 264
246 202 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1554 1554
Size: 4670
BtgI C'CryG_G
Cuts at: 107 852 107
Size: 745 3925
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2620 2801 2964 2620
Size: 181 163 4326
Fragments arranged by size:
4326 181 163
BtsI GCAGTG_nn'
Cuts at: 695 1148 1913 695
Size: 453 765 3452
Fragments arranged by size:
3452 765 453
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 2376 3234 2376
Size: 858 3812
EaeI y'GGCC_r
Cuts at: 428 971 1094 2255 2429 2820 2847 4192 428
Size: 543 123 1161 174 391 27 1345 906
Fragments arranged by size:
1345 1161 906 543 391 174 123 27
EagI C'GGCC_G
Cuts at: 2255 2255
Size: 4670
EarI CTCTTCn'nnn_
Cuts at: 379 1601 2693 2903 379
Size: 1222 1092 210 2146
Fragments arranged by size:
2146 1222 1092 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2878 3188 3334 4480 2878
Size: 310 146 1146 3068
Fragments arranged by size:
3068 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1125 1682 1125
Size: 557 4113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 571 667 1375 1511 1583 1624 2495 2927
Size: 401 96 708 136 72 41 871 432
Cuts at: 2927 3674 3942 170
Size: 747 268 898
Fragments arranged by size:
898 871 747 708 432 401 268 136
96 72 41
EcoNI CCTnn'n_nnAGG
Cuts at: 421 1321 421
Size: 900 3770
EcoO109I rG'GnC_Cy
Cuts at: 1809 1809
Size: 4670
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 298 657 840 2689 298
Size: 359 183 1849 2279
Fragments arranged by size:
2279 1849 359 183
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 2104 2352 3374 4353 2104
Size: 248 1022 979 2421
Fragments arranged by size:
2421 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 120 152 1318 1350 1423 1455 2512 2542 2544
Size: 32 1166 32 73 32 1057 30 2
Cuts at: 2544 2574 120
Size: 30 2216
Fragments arranged by size:
2216 1166 1057 73 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1657 3341 3525 1657
Size: 1684 184 2802
Fragments arranged by size:
2802 1684 184
MscI TGG'CCA
Cuts at: 430 2431 430
Size: 2001 2669
MslI CAynn'nnrTG
Cuts at: 937 2659 2786 937
Size: 1722 127 2821
Fragments arranged by size:
2821 1722 127
MspA1I CmG'CkG
Cuts at: 980 1534 1781 2095 2455 3468 3713 980
Size: 554 247 314 360 1013 245 1937
Fragments arranged by size:
1937 1013 554 360 314 247 245
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 4670
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NcoI C'CATG_G
Cuts at: 107 107
Size: 4670
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 4670
NruI TCG'CGA
Cuts at: 127 127
Size: 4670
NsiI A_TGCA'T
Cuts at: 1071 1071
Size: 4670
NspI r_CATG'y
Cuts at: 386 1134 1723 2223 3130 386
Size: 748 589 500 907 1926
Fragments arranged by size:
1926 907 748 589 500
PciI A'CATG_T
Cuts at: 382 3126 382
Size: 2744 1926
PflMI CCAn_nnn'nTGG
Cuts at: 645 645
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PstI C_TGCA'G
Cuts at: 1717 2402 1717
Size: 685 3985
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 980 1534 2095 2455 980
Size: 554 561 360 3195
Fragments arranged by size:
3195 561 554 360
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1127 1684 1127
Size: 557 4113
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SfcI C'TryA_G
Cuts at: 33 1713 2398 3391 3582 33
Size: 1680 685 993 191 1121
Fragments arranged by size:
1680 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 1692 1692
Size: 4670
SmlI C'TyrA_G
Cuts at: 418 1321 1825 2740 3232 3494 418
Size: 903 504 915 492 262 1594
Fragments arranged by size:
1594 915 903 504 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
StyI C'CwwG_G
Cuts at: 107 528 1804 2158 107
Size: 421 1276 354 2619
Fragments arranged by size:
2619 1276 421 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2253 2386 2845 3054 2253
Size: 133 459 209 3869
Fragments arranged by size:
3869 459 209 133
TatI w'GTAC_w
Cuts at: 1253 4185 1253
Size: 2932 1738
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4187 4219 4187
Size: 32 4638
Tth111I GACn'n_nGTC
Cuts at: 2467 2467
Size: 4670
XbaI T'CTAG_A
Cuts at: 1701 1701
Size: 4670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2165 2165
Size: 4670
XmaI C'CCGG_G
Cuts at: 1690 1690
Size: 4670
XmnI GAAnn'nnTTC
Cuts at: 281 556 281
Size: 275 4395
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NruI NsiI NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI ApaI AscI BaeI
BcgI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI