Restriction map for
pF1KB5979
(Circular) (Six-base) MAPSORT of: pF1KB5979.seq Check: 5003 from: 1 to: 4673
pF1KB5979 4673 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1406 2498 2930 3677 3945 857
Size: 549 1092 432 747 268 1585
Fragments arranged by size:
1585 1092 747 549 432 268
AfeI AGC'GCT
Cuts at: 258 258
Size: 4673
AflIII A'CryG_T
Cuts at: 2025 3129 4106 2025
Size: 1104 977 2592
Fragments arranged by size:
2592 1104 977
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1518 1518
Size: 4673
AlwNI CAG_nnn'CTG
Cuts at: 1978 3545 1978
Size: 1567 3106
ApaI G_GGCC'C
Cuts at: 1101 1101
Size: 4673
ApaLI G'TGCA_C
Cuts at: 778 3443 778
Size: 2665 2008
ApoI r'AATT_y
Cuts at: 1677 4026 4282 1677
Size: 2349 256 2068
Fragments arranged by size:
2349 2068 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 122 660 773 1693 122
Size: 538 113 920 3102
Fragments arranged by size:
3102 920 538 113
BamHI G'GATC_C
Cuts at: 504 1698 504
Size: 1194 3479
BanI G'GyrC_C
Cuts at: 540 1689 2103 2351 2386 540
Size: 1149 414 248 35 2827
Fragments arranged by size:
2827 1149 414 248 35
BanII G_rGCy'C
Cuts at: 121 305 1101 1687 2717 121
Size: 184 796 586 1030 2077
Fragments arranged by size:
2077 1030 796 586 184
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 1394 1394
Size: 4673
BciVI GTATCCnnnnn_n'
Cuts at: 1655 2571 3332 4673 1655
Size: 916 761 1341 1655
Fragments arranged by size:
1655 1341 916 761
BclI T'GATC_A
Cuts at: 443 1398 2193 443
Size: 955 795 2923
Fragments arranged by size:
2923 955 795
BfrBI ATG'CAT
Cuts at: 759 759
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 782 1101 2298 2391 3447 782
Size: 319 1197 93 1056 2008
Fragments arranged by size:
2008 1197 1056 319 93
BmrI ACTGGGnnnn_n'
Cuts at: 1885 1966 2301 4445 1885
Size: 81 335 2144 2113
Fragments arranged by size:
2144 2113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1377 1061
Size: 316 4357
Bpu10I CC'TnA_GC
Cuts at: 206 206
Size: 4673
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1849 2728 3220 3518 1849
Size: 879 492 298 3004
Fragments arranged by size:
3004 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 153 661 988 4377 153
Size: 508 327 3389 449
Fragments arranged by size:
3389 508 449 327
BsaAI yAC'GTr
Cuts at: 783 2026 783
Size: 1243 3430
BsaBI GATnn'nnATC
Cuts at: 6 1403 2187 4224 6
Size: 1397 784 2037 455
Fragments arranged by size:
2037 1397 784 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 256 253
Size: 3 4670
BseYI C'CCAG_C
Cuts at: 163 598 2098 2856 3433 3929 163
Size: 435 1500 758 577 496 907
Fragments arranged by size:
1500 907 758 577 496 435
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 121 782 1687 2465 2655 3447 121
Size: 661 905 778 190 792 1347
Fragments arranged by size:
1347 905 792 778 661 190
BsmI GAATG_Cn'
Cuts at: 1181 1181
Size: 4673
Bsp1286I G_dGCh'C
Cuts at: 121 305 782 1101 1687 2298 2391 2465 2655
Size: 184 477 319 586 611 93 74 190
Cuts at: 2655 2717 3447 121
Size: 62 730 1347
Fragments arranged by size:
1347 730 611 586 477 319 190 184
93 74 62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 1325 3849 1325
Size: 2524 2149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1205 1220 1723 1937 2239 2620 1205
Size: 15 503 214 302 381 3258
Fragments arranged by size:
3258 503 381 302 214 15
BsrBI CCG'CTC
Cuts at: 50 2965 3019 4503 4668 50
Size: 2915 54 1484 165 55
Fragments arranged by size:
2915 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 543 1272 2671 2852 3061 543
Size: 729 1399 181 209 2155
Fragments arranged by size:
2155 1399 729 209 181
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 756 2050 756
Size: 1294 3379
BstBI TT'CG_AA
Cuts at: 3034 3034
Size: 4673
BstEII G'GTnAC_C
Cuts at: 1359 1359
Size: 4673
BstXI CCAn_nnnn'nTGG
Cuts at: 537 537
Size: 4673
BstYI r'GATC_y
Cuts at: 1 400 504 1698 2165 2188 2523 2769 3781
Size: 399 104 1194 467 23 335 246 1012
Cuts at: 3781 3867 3879 4225 1
Size: 86 12 346 449
Fragments arranged by size:
1194 1012 467 449 399 346 335 246
104 86 23 12
BtgI C'CryG_G
Cuts at: 107 140 1003 107
Size: 33 863 3777
Fragments arranged by size:
3777 863 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 807 1071 1916 807
Size: 264 845 3564
Fragments arranged by size:
3564 845 264
ClaI AT'CG_AT
Cuts at: 533 533
Size: 4673
DraI TTT'AAA
Cuts at: 861 1032 1672 861
Size: 171 640 3862
Fragments arranged by size:
3862 640 171
DraIII CAC_nnn'GTG
Cuts at: 1057 1057
Size: 4673
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 527 2258 2432 2823 2850 4195 527
Size: 1731 174 391 27 1345 1005
Fragments arranged by size:
1731 1345 1005 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 230 262 365 2696 2906 230
Size: 32 103 2331 210 1997
Fragments arranged by size:
2331 1997 210 103 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 211 242 480 2881 3191 3337 4483 211
Size: 31 238 2401 310 146 1146 401
Fragments arranged by size:
2401 1146 401 310 238 146 31
EcoICRI GAG'CTC
Cuts at: 119 1685 119
Size: 1566 3107
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1061 1377 1406 2498 2930 3677 3945 857
Size: 204 316 29 1092 432 747 268 1585
Fragments arranged by size:
1585 1092 747 432 316 268 204 29
EcoO109I rG'GnC_Cy
Cuts at: 1097 1098 1812 1097
Size: 1 714 3958
Fragments arranged by size:
3958 714 1
EcoRI G'AATT_C
Cuts at: 1677 1677
Size: 4673
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 950 2692 950
Size: 1742 2931
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 260 2107 2355 3377 4356 260
Size: 1847 248 1022 979 577
Fragments arranged by size:
1847 1022 979 577 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2515 2545 2547 2577 2515
Size: 30 2 30 4611
Fragments arranged by size:
4611 30 30 2
HincII GTy'rAC
Cuts at: 807 1209 1712 807
Size: 402 503 3768
Fragments arranged by size:
3768 503 402
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 1261 1598 3344 3528 482
Size: 779 337 1746 184 1627
Fragments arranged by size:
1746 1627 779 337 184
MscI TGG'CCA
Cuts at: 529 2434 529
Size: 1905 2768
MslI CAynn'nnrTG
Cuts at: 535 2662 2789 535
Size: 2127 127 2419
Fragments arranged by size:
2419 2127 127
MspA1I CmG'CkG
Cuts at: 341 1784 2098 2458 3471 3716 341
Size: 1443 314 360 1013 245 1298
Fragments arranged by size:
1443 1298 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 1003 107
Size: 896 3777
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NsiI A_TGCA'T
Cuts at: 761 761
Size: 4673
NspI r_CATG'y
Cuts at: 1726 2226 3133 1726
Size: 500 907 3266
Fragments arranged by size:
3266 907 500
PasI CC'CwG_GG
Cuts at: 1353 1353
Size: 4673
PciI A'CATG_T
Cuts at: 3129 3129
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PmlI CAC'GTG
Cuts at: 783 783
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4641
PshAI GACnn'nnGTC
Cuts at: 1598 1598
Size: 4673
PspOMI G'GGCC_C
Cuts at: 1097 1097
Size: 4673
PstI C_TGCA'G
Cuts at: 264 1720 2405 264
Size: 1456 685 2532
Fragments arranged by size:
2532 1456 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 2098 2458 2098
Size: 360 4313
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 121 1687 121
Size: 1566 3107
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 262 2696 2906 262
Size: 2434 210 2029
Fragments arranged by size:
2434 2029 210
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SfcI C'TryA_G
Cuts at: 33 260 561 1716 2401 3394 3585 33
Size: 227 301 1155 685 993 191 1121
Fragments arranged by size:
1155 1121 993 685 301 227 191
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 1828 2743 3235 3497 1828
Size: 915 492 262 3004
Fragments arranged by size:
3004 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
SspI AAT'ATT
Cuts at: 1464 1464
Size: 4673
StuI AGG'CCT
Cuts at: 1011 1011
Size: 4673
StyI C'CwwG_G
Cuts at: 107 149 1003 1170 1807 2161 107
Size: 42 854 167 637 354 2619
Fragments arranged by size:
2619 854 637 354 167 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2256 2389 2848 3057 2256
Size: 133 459 209 3872
Fragments arranged by size:
3872 459 209 133
TatI w'GTAC_w
Cuts at: 764 4188 764
Size: 3424 1249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 2470 2470
Size: 4673
XbaI T'CTAG_A
Cuts at: 1555 1704 1555
Size: 149 4524
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 720 1244 2168 720
Size: 524 924 3225
Fragments arranged by size:
3225 924 524
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
XmnI GAAnn'nnTTC
Cuts at: 418 418
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PmeI PmlI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PflMI PfoI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SwaI XhoI ZraI