Restriction map for
pF1KB5980
(Circular) (Six-base) MAPSORT of: pF1KB5980.seq Check: 4055 from: 1 to: 4814
pF1KB5980 4814 bp
With 182 enzymes: SgfI *
November 27, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 678 678
Size: 4814
AccI GT'mk_AC
Cuts at: 1852 1852
Size: 4814
Acc65I G'GTAC_C
Cuts at: 341 1830 341
Size: 1489 3325
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 1043 2639 3071 3818 4086 540
Size: 503 1596 432 747 268 1268
Fragments arranged by size:
1596 1268 747 503 432 268
AfeI AGC'GCT
Cuts at: 415 415
Size: 4814
AflIII A'CryG_T
Cuts at: 326 2166 3270 4247 326
Size: 1840 1104 977 893
Fragments arranged by size:
1840 1104 977 893
AgeI A'CCGG_T
Cuts at: 222 886 3202 222
Size: 664 2316 1834
Fragments arranged by size:
2316 1834 664
AleI CACnn'nnGTG
Cuts at: 223 223
Size: 4814
AlwNI CAG_nnn'CTG
Cuts at: 237 654 693 981 2119 3686 237
Size: 417 39 288 1138 1567 1365
Fragments arranged by size:
1567 1365 1138 417 288 39
ApaI G_GGCC'C
Cuts at: 188 188
Size: 4814
ApaLI G'TGCA_C
Cuts at: 1354 3584 1354
Size: 2230 2584
ApoI r'AATT_y
Cuts at: 1818 4167 4423 1818
Size: 2349 256 2209
Fragments arranged by size:
2349 2209 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 187 195 751 1072 1119 1604 1834 187
Size: 8 556 321 47 485 230 3167
Fragments arranged by size:
3167 556 485 321 230 47 8
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 225 341 791 831 1078 1264 1417 1830 2244
Size: 116 450 40 247 186 153 413 414
Cuts at: 2244 2492 2527 225
Size: 248 35 2512
Fragments arranged by size:
2512 450 414 413 248 247 186 153
116 40 35
BanII G_rGCy'C
Cuts at: 188 202 256 1150 1298 1828 2858 188
Size: 14 54 894 148 530 1030 2144
Fragments arranged by size:
2144 1030 894 530 148 54 14
BbeI G_GCGC'C
Cuts at: 795 835 1082 1421 2248 2496 795
Size: 40 247 339 827 248 3113
Fragments arranged by size:
3113 827 339 248 247 40
BbsI GAAGACnn'nnnn_
Cuts at: 281 1379 1520 281
Size: 1098 141 3575
Fragments arranged by size:
3575 1098 141
BbvCI CC'TCA_GC
Cuts at: 1010 1138 1010
Size: 128 4686
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1242 1276 1449 1483 1242
Size: 34 173 34 4573
Fragments arranged by size:
4573 173 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2712 3473 4814 2712
Size: 761 1341 2712
Fragments arranged by size:
2712 1341 761
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglI GCCn_nnn'nGGC
Cuts at: 251 994 1006 1462 251
Size: 743 12 456 3603
Fragments arranged by size:
3603 743 456 12
BglII A'GATC_T
Cuts at: 2329 4366 2329
Size: 2037 2777
BlpI GC'TnA_GC
Cuts at: 1926 1926
Size: 4814
Bme1580I G_kGCm'C
Cuts at: 188 665 1358 2439 2532 3588 188
Size: 477 693 1081 93 1056 1414
Fragments arranged by size:
1414 1081 1056 693 477 93
BmgBI CAC'GTC
Cuts at: 327 327
Size: 4814
BmrI ACTGGGnnnn_n'
Cuts at: 370 506 1668 2026 2107 2442 4586 370
Size: 136 1162 358 81 335 2144 598
Fragments arranged by size:
2144 1162 598 358 335 136 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 973 1003 1312 1467 1529 1771 619
Size: 354 30 309 155 62 242 3662
Fragments arranged by size:
3662 354 309 242 155 62 30
Bpu10I CC'TnA_GC
Cuts at: 1010 1138 1010
Size: 128 4686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1044 1636 1990 2869 3361 3659 1044
Size: 592 354 879 492 298 2199
Fragments arranged by size:
2199 879 592 492 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 523 1222 4518 523
Size: 699 3296 819
Fragments arranged by size:
3296 819 699
BsaAI yAC'GTr
Cuts at: 348 2167 348
Size: 1819 2995
BsaBI GATnn'nnATC
Cuts at: 6 1318 1324 2328 4365 6
Size: 1312 6 1004 2037 455
Fragments arranged by size:
2037 1312 1004 455 6
BsaHI Gr'CG_yC
Cuts at: 792 832 1079 1418 2245 2493 3195 4691 792
Size: 40 247 339 827 248 702 1496 915
Fragments arranged by size:
1496 915 827 702 339 248 247 40
BsaWI w'CCGG_w
Cuts at: 222 886 1619 2003 2524 3202 3476 3623 4034
Size: 664 733 384 521 678 274 147 411
Cuts at: 4034 222
Size: 1002
Fragments arranged by size:
1002 733 678 664 521 411 384 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 569 599 569
Size: 30 4784
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 970 1640 610
Size: 360 670 3784
Fragments arranged by size:
3784 670 360
BseYI C'CCAG_C
Cuts at: 365 395 606 1098 1349 1575 2239 2997 3574
Size: 30 211 492 251 226 664 758 577
Cuts at: 3574 4070 365
Size: 496 1109
Fragments arranged by size:
1109 758 664 577 496 492 251 226
211 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 679 679
Size: 4814
BsiEI CG_ry'CG
Cuts at: 104 1131 2356 2402 3610 104
Size: 1027 1225 46 1208 1308
Fragments arranged by size:
1308 1225 1208 1027 46
BsiHKAI G_wGCw'C
Cuts at: 1358 1453 1828 2606 2796 3588 1358
Size: 95 375 778 190 792 2584
Fragments arranged by size:
2584 792 778 375 190 95
BsmI GAATG_Cn'
Cuts at: 1503 1503
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 188 202 256 665 1150 1298 1358 1453 1828
Size: 14 54 409 485 148 60 95 375
Cuts at: 1828 2439 2532 2606 2796 2858 3588 188
Size: 611 93 74 190 62 730 1414
Fragments arranged by size:
1414 730 611 485 409 375 190 148
95 93 74 62 60 54 14
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 3990 3990
Size: 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 678 980 1078 1864 2078 2380 2761 678
Size: 302 98 786 214 302 381 2731
Fragments arranged by size:
2731 786 381 302 302 214 98
BsrBI CCG'CTC
Cuts at: 50 848 960 3106 3160 4644 4809 50
Size: 798 112 2146 54 1484 165 55
Fragments arranged by size:
2146 1484 798 165 112 55 54
BsrDI GCAATG_nn'
Cuts at: 2726 2726
Size: 4814
BsrFI r'CCGG_y
Cuts at: 222 886 2812 2993 3202 222
Size: 664 1926 181 209 1834
Fragments arranged by size:
1926 1834 664 209 181
BssSI C'ACGA_G
Cuts at: 3085 3443 3085
Size: 358 4456
BstAPI GCAn_nnn'nTGC
Cuts at: 693 1346 1457 2191 693
Size: 653 111 734 3316
Fragments arranged by size:
3316 734 653 111
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstYI r'GATC_y
Cuts at: 1 560 1839 2306 2329 2664 2910 3922 4008
Size: 559 1279 467 23 335 246 1012 86
Cuts at: 4008 4020 4366 1
Size: 12 346 449
Fragments arranged by size:
1279 1012 559 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1158 1158
Size: 4814
BtgI C'CryG_G
Cuts at: 809 989 809
Size: 180 4634
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1524 2764 2945 3108 1524
Size: 1240 181 163 3230
Fragments arranged by size:
3230 1240 181 163
BtsI GCAGTG_nn'
Cuts at: 860 2057 860
Size: 1197 3617
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DraIII CAC_nnn'GTG
Cuts at: 312 351 722 815 1551 312
Size: 39 371 93 736 3575
Fragments arranged by size:
3575 736 371 93 39
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 215 1128 1475 2399 2573 2964 2991 4336 215
Size: 913 347 924 174 391 27 1345 693
Fragments arranged by size:
1345 924 913 693 391 347 174 27
EagI C'GGCC_G
Cuts at: 1128 2399 1128
Size: 1271 3543
EarI CTCTTCn'nnn_
Cuts at: 433 667 1062 1110 1183 2837 3047 433
Size: 234 395 48 73 1654 210 2200
Fragments arranged by size:
2200 1654 395 234 210 73 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3022 3332 3478 4624 3022
Size: 310 146 1146 3212
Fragments arranged by size:
3212 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 540 619 973 1003 1043 1312 1467 1529 1771
Size: 79 354 30 40 269 155 62 242
Cuts at: 1771 2639 3071 3818 4086 540
Size: 868 432 747 268 1268
Fragments arranged by size:
1268 868 747 432 354 269 268 242
155 79 62 40 30
EcoNI CCTnn'n_nnAGG
Cuts at: 1669 1705 1669
Size: 36 4778
EcoO109I rG'GnC_Cy
Cuts at: 243 651 969 1090 1236 1662 1953 243
Size: 408 318 121 146 426 291 3104
Fragments arranged by size:
3104 426 408 318 291 146 121
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 355 2595 355
Size: 2240 2574
HaeII r_GCGC'y
Cuts at: 116 417 795 835 1082 1421 1727 2248 2496
Size: 301 378 40 247 339 306 521 248
Cuts at: 2496 3518 4497 116
Size: 1022 979 433
Fragments arranged by size:
1022 979 521 433 378 339 306 301
248 247 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2656 2686 2688 2718 2656
Size: 30 2 30 4752
Fragments arranged by size:
4752 30 30 2
HincII GTy'rAC
Cuts at: 1853 1853
Size: 4814
KasI G'GCGC_C
Cuts at: 791 831 1078 1417 2244 2492 791
Size: 40 247 339 827 248 3113
Fragments arranged by size:
3113 827 339 248 247 40
KpnI G_GTAC'C
Cuts at: 345 1834 345
Size: 1489 3325
MluI A'CGCG_T
Cuts at: 4247 4247
Size: 4814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 281 665 3485 3669 281
Size: 384 2820 184 1426
Fragments arranged by size:
2820 1426 384 184
MscI TGG'CCA
Cuts at: 217 1477 2575 217
Size: 1260 1098 2456
Fragments arranged by size:
2456 1260 1098
MslI CAynn'nnrTG
Cuts at: 223 2803 2930 223
Size: 2580 127 2107
Fragments arranged by size:
2580 2107 127
MspA1I CmG'CkG
Cuts at: 140 149 466 1043 1777 1925 2239 2599 3612
Size: 9 317 577 734 148 314 360 1013
Cuts at: 3612 3857 140
Size: 245 1097
Fragments arranged by size:
1097 1013 734 577 360 317 314 245
148 9
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 792 832 1079 1418 2245 2493 792
Size: 40 247 339 827 248 3113
Fragments arranged by size:
3113 827 339 248 247 40
NcoI C'CATG_G
Cuts at: 989 989
Size: 4814
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 1867 2367 3274 1867
Size: 500 907 3407
Fragments arranged by size:
3407 907 500
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 386 815 386
Size: 429 4385
PfoI T'CCnGG_A
Cuts at: 497 497
Size: 4814
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PmlI CAC'GTG
Cuts at: 348 348
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4782
PpuMI rG'GwC_Cy
Cuts at: 969 1090 969
Size: 121 4693
PshAI GACnn'nnGTC
Cuts at: 535 1520 535
Size: 985 3829
PspOMI G'GGCC_C
Cuts at: 184 184
Size: 4814
PstI C_TGCA'G
Cuts at: 242 977 1861 2546 242
Size: 735 884 685 2510
Fragments arranged by size:
2510 884 735 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 466 2239 2599 466
Size: 1773 360 2681
Fragments arranged by size:
2681 1773 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SapI GCTCTTCn'nnn_
Cuts at: 667 2837 3047 667
Size: 2170 210 2434
Fragments arranged by size:
2434 2170 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 238 973 1518 1857 2542 3535 3726 33
Size: 205 735 545 339 685 993 191 1121
Fragments arranged by size:
1121 993 735 685 545 339 205 191
SfoI GGC'GCC
Cuts at: 793 833 1080 1419 2246 2494 793
Size: 40 247 339 827 248 3113
Fragments arranged by size:
3113 827 339 248 247 40
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SgrAI Cr'CCGG_yG
Cuts at: 222 222
Size: 4814
SmaI CCC'GGG
Cuts at: 1074 1121 1606 1836 1074
Size: 47 485 230 4052
Fragments arranged by size:
4052 485 230 47
SmlI C'TyrA_G
Cuts at: 1023 1651 1969 2884 3376 3638 1023
Size: 628 318 915 492 262 2199
Fragments arranged by size:
2199 915 628 492 318 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StuI AGG'CCT
Cuts at: 175 175
Size: 4814
StyI C'CwwG_G
Cuts at: 989 1948 2302 989
Size: 959 354 3501
Fragments arranged by size:
3501 959 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1018 1341 2397 2530 2989 3198 1018
Size: 323 1056 133 459 209 2634
Fragments arranged by size:
2634 1056 459 323 209 133
TatI w'GTAC_w
Cuts at: 1748 4329 1748
Size: 2581 2233
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 569 601 4331 4363 569
Size: 32 3730 32 1020
Fragments arranged by size:
3730 1020 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 1845 1845
Size: 4814
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2309 2309
Size: 4814
XmaI C'CCGG_G
Cuts at: 1072 1119 1604 1834 1072
Size: 47 485 230 4052
Fragments arranged by size:
4052 485 230 47
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI