Restriction map for
pF1KB5982
(Circular) (Six-base) MAPSORT of: pF1KB5982.seq Check: 4260 from: 1 to: 4817
pF1KB5982 4817 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1855 1855
Size: 4817
Acc65I G'GTAC_C
Cuts at: 1833 1833
Size: 4817
AclI AA'CG_TT
Cuts at: 4657 4657
Size: 4817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 617 704 2642 3074 3821 4089 430
Size: 187 87 1938 432 747 268 1158
Fragments arranged by size:
1938 1158 747 432 268 187 87
AfeI AGC'GCT
Cuts at: 1300 1777 1300
Size: 477 4340
AflIII A'CryG_T
Cuts at: 396 2169 3273 4250 396
Size: 1773 1104 977 963
Fragments arranged by size:
1773 1104 977 963
AgeI A'CCGG_T
Cuts at: 3205 3205
Size: 4817
AleI CACnn'nnGTG
Cuts at: 545 545
Size: 4817
AlwNI CAG_nnn'CTG
Cuts at: 2122 3689 2122
Size: 1567 3250
ApaLI G'TGCA_C
Cuts at: 3587 3587
Size: 4817
ApoI r'AATT_y
Cuts at: 362 668 1428 1821 4170 4426 362
Size: 306 760 393 2349 256 753
Fragments arranged by size:
2349 760 753 393 306 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4817
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
AvaI C'yCGr_G
Cuts at: 1352 1837 1352
Size: 485 4332
AvrII C'CTAG_G
Cuts at: 936 936
Size: 4817
BamHI G'GATC_C
Cuts at: 1842 1842
Size: 4817
BanI G'GyrC_C
Cuts at: 1360 1833 2247 2495 2530 1360
Size: 473 414 248 35 3647
Fragments arranged by size:
3647 473 414 248 35
BanII G_rGCy'C
Cuts at: 305 933 1831 2861 305
Size: 628 898 1030 2261
Fragments arranged by size:
2261 1030 898 628
BbeI G_GCGC'C
Cuts at: 2251 2499 2251
Size: 248 4569
BbvCI CC'TCA_GC
Cuts at: 569 569
Size: 4817
BciVI GTATCCnnnnn_n'
Cuts at: 2715 3476 4817 2715
Size: 761 1341 2715
Fragments arranged by size:
2715 1341 761
BclI T'GATC_A
Cuts at: 2337 2337
Size: 4817
BglII A'GATC_T
Cuts at: 2332 4369 2332
Size: 2037 2780
BlpI GC'TnA_GC
Cuts at: 821 1929 821
Size: 1108 3709
Bme1580I G_kGCm'C
Cuts at: 2442 2535 3591 2442
Size: 93 1056 3668
Fragments arranged by size:
3668 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 723 2029 2110 2445 4589 723
Size: 1306 81 335 2144 951
Fragments arranged by size:
2144 1306 951 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3184 3184
Size: 4817
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1244 1280 1244
Size: 36 4781
Bpu10I CC'TnA_GC
Cuts at: 569 569
Size: 4817
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1993 2872 3364 3662 1993
Size: 879 492 298 3148
Fragments arranged by size:
3148 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4521 4521
Size: 4817
BsaAI yAC'GTr
Cuts at: 2170 2170
Size: 4817
BsaBI GATnn'nnATC
Cuts at: 6 2331 4368 6
Size: 2325 2037 455
Fragments arranged by size:
2325 2037 455
BsaHI Gr'CG_yC
Cuts at: 1281 2248 2496 3198 4694 1281
Size: 967 248 702 1496 1404
Fragments arranged by size:
1496 1404 967 702 248
BsaWI w'CCGG_w
Cuts at: 1094 2006 2527 3205 3479 3626 4037 1094
Size: 912 521 678 274 147 411 1874
Fragments arranged by size:
1874 912 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1332 1745 1772 2242 3000 3577 4073 1332
Size: 413 27 470 758 577 496 2076
Fragments arranged by size:
2076 758 577 496 470 413 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1523 1523
Size: 4817
BsiEI CG_ry'CG
Cuts at: 104 2359 2405 3613 104
Size: 2255 46 1208 1308
Fragments arranged by size:
2255 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 305 1831 2609 2799 3591 305
Size: 1526 778 190 792 1531
Fragments arranged by size:
1531 1526 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1056 1056
Size: 4817
Bsp1286I G_dGCh'C
Cuts at: 305 933 1831 2442 2535 2609 2799 2861 3591
Size: 628 898 611 93 74 190 62 730
Cuts at: 3591 305
Size: 1531
Fragments arranged by size:
1531 898 730 628 611 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4037 4037
Size: 4817
BspHI T'CATG_A
Cuts at: 1089 3993 1089
Size: 2904 1913
BspMI ACCTGCnnnn'nnnn_
Cuts at: 767 1867 2081 2383 2764 767
Size: 1100 214 302 381 2820
Fragments arranged by size:
2820 1100 381 302 214
BsrBI CCG'CTC
Cuts at: 50 738 3109 3163 4647 4812 50
Size: 688 2371 54 1484 165 55
Fragments arranged by size:
2371 1484 688 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2729 2729
Size: 4817
BsrFI r'CCGG_y
Cuts at: 2815 2996 3205 2815
Size: 181 209 4427
Fragments arranged by size:
4427 209 181
BsrGI T'GTAC_A
Cuts at: 380 414 380
Size: 34 4783
BssSI C'ACGA_G
Cuts at: 3088 3446 3088
Size: 358 4459
BstAPI GCAn_nnn'nTGC
Cuts at: 2194 2194
Size: 4817
BstBI TT'CG_AA
Cuts at: 3178 3178
Size: 4817
BstXI CCAn_nnnn'nTGG
Cuts at: 999 1236 999
Size: 237 4580
BstYI r'GATC_y
Cuts at: 1 855 1097 1842 2309 2332 2667 2913 3925
Size: 854 242 745 467 23 335 246 1012
Cuts at: 3925 4011 4023 4369 1
Size: 86 12 346 449
Fragments arranged by size:
1012 854 745 467 449 346 335 246
242 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 184 184
Size: 4817
BtgI C'CryG_G
Cuts at: 107 356 1763 107
Size: 249 1407 3161
Fragments arranged by size:
3161 1407 249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2767 2948 3111 2767
Size: 181 163 4473
Fragments arranged by size:
4473 181 163
BtsI GCAGTG_nn'
Cuts at: 2060 2060
Size: 4817
DraI TTT'AAA
Cuts at: 1816 1816
Size: 4817
DrdI GACnn_nn'nnGTC
Cuts at: 2523 3381 2523
Size: 858 3959
EaeI y'GGCC_r
Cuts at: 1122 1376 1484 2402 2576 2967 2994 4339 1122
Size: 254 108 918 174 391 27 1345 1600
Fragments arranged by size:
1600 1345 918 391 254 174 108 27
EagI C'GGCC_G
Cuts at: 2402 2402
Size: 4817
EarI CTCTTCn'nnn_
Cuts at: 184 608 680 2840 3050 184
Size: 424 72 2160 210 1951
Fragments arranged by size:
2160 1951 424 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3025 3335 3481 4627 3025
Size: 310 146 1146 3215
Fragments arranged by size:
3215 1146 310 146
EcoICRI GAG'CTC
Cuts at: 303 1829 303
Size: 1526 3291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 617 704 1244 1280 2642 3074 3821 4089
Size: 187 87 540 36 1362 432 747 268
Cuts at: 4089 430
Size: 1158
Fragments arranged by size:
1362 1158 747 540 432 268 187 87
36
EcoNI CCTnn'n_nnAGG
Cuts at: 1454 1454
Size: 4817
EcoO109I rG'GnC_Cy
Cuts at: 916 1956 916
Size: 1040 3777
EcoRI G'AATT_C
Cuts at: 1821 1821
Size: 4817
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 442 2836 442
Size: 2394 2423
FspI TGC'GCA
Cuts at: 2598 2598
Size: 4817
HaeII r_GCGC'y
Cuts at: 1302 1779 2251 2499 3521 4500 1302
Size: 477 472 248 1022 979 1619
Fragments arranged by size:
1619 1022 979 477 472 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 132 164 915 947 1232 1264 1380 1412 2659
Size: 32 751 32 285 32 116 32 1247
Cuts at: 2659 2689 2691 2721 132
Size: 30 2 30 2228
Fragments arranged by size:
2228 1247 751 285 116 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 330 552 1856 330
Size: 222 1304 3291
Fragments arranged by size:
3291 1304 222
KasI G'GCGC_C
Cuts at: 2247 2495 2247
Size: 248 4569
KpnI G_GTAC'C
Cuts at: 1837 1837
Size: 4817
MfeI C'AATT_G
Cuts at: 543 1679 543
Size: 1136 3681
MluI A'CGCG_T
Cuts at: 4250 4250
Size: 4817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 1233 3488 3672 476
Size: 757 2255 184 1621
Fragments arranged by size:
2255 1621 757 184
MscI TGG'CCA
Cuts at: 1378 1486 2578 1378
Size: 108 1092 3617
Fragments arranged by size:
3617 1092 108
MslI CAynn'nnrTG
Cuts at: 545 997 1291 1532 1621 2806 2933 545
Size: 452 294 241 89 1185 127 2429
Fragments arranged by size:
2429 1185 452 294 241 127 89
MspA1I CmG'CkG
Cuts at: 568 836 1745 1928 2242 2602 3615 3860 568
Size: 268 909 183 314 360 1013 245 1525
Fragments arranged by size:
1525 1013 909 360 314 268 245 183
NaeI GCC'GGC
Cuts at: 2998 2998
Size: 4817
NarI GG'CG_CC
Cuts at: 2248 2496 2248
Size: 248 4569
NcoI C'CATG_G
Cuts at: 107 356 1763 107
Size: 249 1407 3161
Fragments arranged by size:
3161 1407 249
NgoMIV G'CCGG_C
Cuts at: 2996 2996
Size: 4817
NspI r_CATG'y
Cuts at: 400 727 1332 1870 2370 3277 400
Size: 327 605 538 500 907 1940
Fragments arranged by size:
1940 907 605 538 500 327
PasI CC'CwG_GG
Cuts at: 1236 1236
Size: 4817
PciI A'CATG_T
Cuts at: 396 3273 396
Size: 2877 1940
PfoI T'CCnGG_A
Cuts at: 1603 1603
Size: 4817
PmeI GTTT'AAAC
Cuts at: 1816 1816
Size: 4817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4785
PpuMI rG'GwC_Cy
Cuts at: 916 916
Size: 4817
PshAI GACnn'nnGTC
Cuts at: 1612 1612
Size: 4817
PstI C_TGCA'G
Cuts at: 435 1864 2549 435
Size: 1429 685 2703
Fragments arranged by size:
2703 1429 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4817
PvuII CAG'CTG
Cuts at: 568 1745 2242 2602 568
Size: 1177 497 360 2783
Fragments arranged by size:
2783 1177 497 360
RsrII CG'GwC_CG
Cuts at: 3012 3012
Size: 4817
SacI G_AGCT'C
Cuts at: 305 1831 305
Size: 1526 3291
SalI G'TCGA_C
Cuts at: 1854 1854
Size: 4817
SanDI GG'GwC_CC
Cuts at: 916 916
Size: 4817
SapI GCTCTTCn'nnn_
Cuts at: 184 680 2840 3050 184
Size: 496 2160 210 1951
Fragments arranged by size:
2160 1951 496 210
SbfI CC_TGCA'GG
Cuts at: 1864 1864
Size: 4817
ScaI AGT'ACT
Cuts at: 4334 4334
Size: 4817
SfcI C'TryA_G
Cuts at: 33 267 431 763 837 1860 2545 3538 3729
Size: 234 164 332 74 1023 685 993 191
Cuts at: 3729 33
Size: 1121
Fragments arranged by size:
1121 1023 993 685 332 234 191 164
74
SfoI GGC'GCC
Cuts at: 2249 2497 2249
Size: 248 4569
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4817
SmaI CCC'GGG
Cuts at: 1354 1839 1354
Size: 485 4332
SmlI C'TyrA_G
Cuts at: 1972 2887 3379 3641 1972
Size: 915 492 262 3148
Fragments arranged by size:
3148 915 492 262
SpeI A'CTAG_T
Cuts at: 67 295 67
Size: 228 4589
SphI G_CATG'C
Cuts at: 727 1332 1870 2370 727
Size: 605 538 500 3174
Fragments arranged by size:
3174 605 538 500
StuI AGG'CCT
Cuts at: 891 891
Size: 4817
StyI C'CwwG_G
Cuts at: 107 336 356 936 1763 1951 2305 107
Size: 229 20 580 827 188 354 2619
Fragments arranged by size:
2619 827 580 354 229 188 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 705 2400 2533 2992 3201 705
Size: 1695 133 459 209 2321
Fragments arranged by size:
2321 1695 459 209 133
TatI w'GTAC_w
Cuts at: 380 414 4332 380
Size: 34 3918 865
Fragments arranged by size:
3918 865 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1358 1390 1555 1587 4334 4366 1358
Size: 32 165 32 2747 32 1809
Fragments arranged by size:
2747 1809 165 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1214 2614 1214
Size: 1400 3417
XbaI T'CTAG_A
Cuts at: 1152 1848 1152
Size: 696 4121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2312 2312
Size: 4817
XmaI C'CCGG_G
Cuts at: 1352 1837 1352
Size: 485 4332
XmnI GAAnn'nnTTC
Cuts at: 235 425 235
Size: 190 4627
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PshAI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AloI ApaI AscI
BaeI BbsI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsiWI BsmI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PsiI PspOMI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI