(Circular) (Six-base) MAPSORT of: pF1KB5983.seq Check: 5000 from: 1 to: 4676 pF1KB5983 4676 bp With 182 enzymes: SgfI * June 26, 2008 10:45 .. AccI GT'mk_AC Cuts at: 1714 1714 Size: 4676 Acc65I G'GTAC_C Cuts at: 1692 1692 Size: 4676 AclI AA'CG_TT Cuts at: 4516 4516 Size: 4676 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 482 587 659 667 1558 2501 2933 3680 3948 Size: 105 72 8 891 943 432 747 268 Cuts at: 3948 482 Size: 1210 Fragments arranged by size: 1210 943 891 747 432 268 105 72 8 AfeI AGC'GCT Cuts at: 415 1203 415 Size: 788 3888 AflIII A'CryG_T Cuts at: 2028 3132 4109 2028 Size: 1104 977 2595 Fragments arranged by size: 2595 1104 977 AgeI A'CCGG_T Cuts at: 1093 3064 1093 Size: 1971 2705 AlwNI CAG_nnn'CTG Cuts at: 293 1149 1981 3548 293 Size: 856 832 1567 1421 Fragments arranged by size: 1567 1421 856 832 ApaLI G'TGCA_C Cuts at: 3446 3446 Size: 4676 ApoI r'AATT_y Cuts at: 130 136 973 1680 4029 4285 130 Size: 6 837 707 2349 256 521 Fragments arranged by size: 2349 837 707 521 256 6 AseI AT'TA_AT Cuts at: 20 20 Size: 4676 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4676 AvaI C'yCGr_G Cuts at: 1658 1696 1658 Size: 38 4638 BamHI G'GATC_C Cuts at: 1701 1701 Size: 4676 BanI G'GyrC_C Cuts at: 1692 2106 2354 2389 1692 Size: 414 248 35 3979 Fragments arranged by size: 3979 414 248 35 BanII G_rGCy'C Cuts at: 1690 2720 1690 Size: 1030 3646 BbeI G_GCGC'C Cuts at: 2110 2358 2110 Size: 248 4428 BbsI GAAGACnn'nnnn_ Cuts at: 1285 1285 Size: 4676 BbvCI CC'TCA_GC Cuts at: 1211 1211 Size: 4676 BciVI GTATCCnnnnn_n' Cuts at: 2574 3335 4676 2574 Size: 761 1341 2574 Fragments arranged by size: 2574 1341 761 BclI T'GATC_A Cuts at: 2196 2196 Size: 4676 BglI GCCn_nnn'nGGC Cuts at: 1633 1633 Size: 4676 BglII A'GATC_T Cuts at: 2191 4228 2191 Size: 2037 2639 BlpI GC'TnA_GC Cuts at: 1127 1788 1127 Size: 661 4015 Bme1580I G_kGCm'C Cuts at: 1467 2301 2394 3450 1467 Size: 834 93 1056 2693 Fragments arranged by size: 2693 1056 834 93 BmrI ACTGGGnnnn_n' Cuts at: 1031 1888 1969 2304 4448 1031 Size: 857 81 335 2144 1259 Fragments arranged by size: 2144 1259 857 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1047 1410 3043 1047 Size: 363 1633 2680 Fragments arranged by size: 2680 1633 363 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 679 679 Size: 4676 Bpu10I CC'TnA_GC Cuts at: 1211 1211 Size: 4676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1852 2731 3223 3521 1852 Size: 879 492 298 3007 Fragments arranged by size: 3007 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 4380 4380 Size: 4676 BsaAI yAC'GTr Cuts at: 2029 2029 Size: 4676 BsaBI GATnn'nnATC Cuts at: 6 341 2190 4227 6 Size: 335 1849 2037 455 Fragments arranged by size: 2037 1849 455 335 BsaHI Gr'CG_yC Cuts at: 2107 2355 3057 4553 2107 Size: 248 702 1496 2230 Fragments arranged by size: 2230 1496 702 248 BsaWI w'CCGG_w Cuts at: 1093 1865 2386 3064 3338 3485 3896 1093 Size: 772 521 678 274 147 411 1873 Fragments arranged by size: 1873 772 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 405 405 Size: 4676 BseYI C'CCAG_C Cuts at: 293 843 2101 2859 3436 3932 293 Size: 550 1258 758 577 496 1037 Fragments arranged by size: 1258 1037 758 577 550 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1249 1249 Size: 4676 BsiEI CG_ry'CG Cuts at: 104 2218 2264 3472 104 Size: 2114 46 1208 1308 Fragments arranged by size: 2114 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 397 1690 2468 2658 3450 397 Size: 1293 778 190 792 1623 Fragments arranged by size: 1623 1293 792 778 190 Bsp1286I G_dGCh'C Cuts at: 397 1467 1690 2301 2394 2468 2658 2720 3450 Size: 1070 223 611 93 74 190 62 730 Cuts at: 3450 397 Size: 1623 Fragments arranged by size: 1623 1070 730 611 223 190 93 74 62 BspEI T'CCGG_A Cuts at: 3896 3896 Size: 4676 BspHI T'CATG_A Cuts at: 3852 3852 Size: 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 410 1726 1940 2242 2623 410 Size: 1316 214 302 381 2463 Fragments arranged by size: 2463 1316 381 302 214 BsrBI CCG'CTC Cuts at: 50 2968 3022 4506 4671 50 Size: 2918 54 1484 165 55 Fragments arranged by size: 2918 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1138 2588 1138 Size: 1450 3226 BsrFI r'CCGG_y Cuts at: 1093 1223 2674 2855 3064 1093 Size: 130 1451 181 209 2705 Fragments arranged by size: 2705 1451 209 181 130 BssSI C'ACGA_G Cuts at: 2947 3305 2947 Size: 358 4318 BstAPI GCAn_nnn'nTGC Cuts at: 2053 2053 Size: 4676 BstBI TT'CG_AA Cuts at: 3037 3037 Size: 4676 BstEII G'GTnAC_C Cuts at: 1429 1429 Size: 4676 BstXI CCAn_nnnn'nTGG Cuts at: 277 277 Size: 4676 BstYI r'GATC_y Cuts at: 1 251 1701 2168 2191 2526 2772 3784 3870 Size: 250 1450 467 23 335 246 1012 86 Cuts at: 3870 3882 4228 1 Size: 12 346 449 Fragments arranged by size: 1450 1012 467 449 346 335 250 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 388 388 Size: 4676 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2626 2807 2970 2626 Size: 181 163 4332 Fragments arranged by size: 4332 181 163 BtsI GCAGTG_nn' Cuts at: 1919 1919 Size: 4676 DraI TTT'AAA Cuts at: 135 1675 135 Size: 1540 3136 DrdI GACnn_nn'nnGTC Cuts at: 2382 3240 2382 Size: 858 3818 EaeI y'GGCC_r Cuts at: 2261 2435 2826 2853 4198 2261 Size: 174 391 27 1345 2739 Fragments arranged by size: 2739 1345 391 174 27 EagI C'GGCC_G Cuts at: 2261 2261 Size: 4676 EarI CTCTTCn'nnn_ Cuts at: 1264 2699 2909 1264 Size: 1435 210 3031 Fragments arranged by size: 3031 1435 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1031 1230 2884 3194 3340 4486 1031 Size: 199 1654 310 146 1146 1221 Fragments arranged by size: 1654 1221 1146 310 199 146 EcoICRI GAG'CTC Cuts at: 1688 1688 Size: 4676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 482 587 659 667 679 1558 2501 2933 3680 Size: 105 72 8 12 879 943 432 747 Cuts at: 3680 3948 482 Size: 268 1210 Fragments arranged by size: 1210 943 879 747 432 268 105 72 12 8 EcoNI CCTnn'n_nnAGG Cuts at: 868 868 Size: 4676 EcoO109I rG'GnC_Cy Cuts at: 1389 1815 1389 Size: 426 4250 EcoRI G'AATT_C Cuts at: 1680 1680 Size: 4676 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1536 2695 1536 Size: 1159 3517 FspI TGC'GCA Cuts at: 2457 2457 Size: 4676 HaeII r_GCGC'y Cuts at: 417 1205 1224 2110 2358 3380 4359 417 Size: 788 19 886 248 1022 979 734 Fragments arranged by size: 1022 979 886 788 734 248 19 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 472 504 780 812 2518 2548 2550 2580 472 Size: 32 276 32 1706 30 2 30 2568 Fragments arranged by size: 2568 1706 276 32 32 30 30 2 HincII GTy'rAC Cuts at: 861 1715 861 Size: 854 3822 HindIII A'AGCT_T Cuts at: 1402 1402 Size: 4676 KasI G'GCGC_C Cuts at: 2106 2354 2106 Size: 248 4428 KpnI G_GTAC'C Cuts at: 1696 1696 Size: 4676 MluI A'CGCG_T Cuts at: 4109 4109 Size: 4676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 262 3347 3531 262 Size: 3085 184 1407 Fragments arranged by size: 3085 1407 184 MscI TGG'CCA Cuts at: 2437 2437 Size: 4676 MslI CAynn'nnrTG Cuts at: 275 829 2665 2792 275 Size: 554 1836 127 2159 Fragments arranged by size: 2159 1836 554 127 MspA1I CmG'CkG Cuts at: 657 1787 2101 2461 3474 3719 657 Size: 1130 314 360 1013 245 1614 Fragments arranged by size: 1614 1130 1013 360 314 245 NaeI GCC'GGC Cuts at: 1225 2857 1225 Size: 1632 3044 NarI GG'CG_CC Cuts at: 2107 2355 2107 Size: 248 4428 NdeI CA'TA_TG Cuts at: 936 936 Size: 4676 NgoMIV G'CCGG_C Cuts at: 1223 2855 1223 Size: 1632 3044 NspI r_CATG'y Cuts at: 820 1251 1729 2229 3136 820 Size: 431 478 500 907 2360 Fragments arranged by size: 2360 907 500 478 431 PciI A'CATG_T Cuts at: 3132 3132 Size: 4676 PfoI T'CCnGG_A Cuts at: 1396 1396 Size: 4676 PmeI GTTT'AAAC Cuts at: 1675 1675 Size: 4676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 3815 Size: 32 4644 PpuMI rG'GwC_Cy Cuts at: 1389 1389 Size: 4676 PstI C_TGCA'G Cuts at: 421 1723 2408 421 Size: 1302 685 2689 Fragments arranged by size: 2689 1302 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4676 PvuII CAG'CTG Cuts at: 657 2101 2461 657 Size: 1444 360 2872 Fragments arranged by size: 2872 1444 360 RsrII CG'GwC_CG Cuts at: 2871 2871 Size: 4676 SacI G_AGCT'C Cuts at: 1690 1690 Size: 4676 SalI G'TCGA_C Cuts at: 1713 1713 Size: 4676 SapI GCTCTTCn'nnn_ Cuts at: 1264 2699 2909 1264 Size: 1435 210 3031 Fragments arranged by size: 3031 1435 210 SbfI CC_TGCA'GG Cuts at: 1723 1723 Size: 4676 ScaI AGT'ACT Cuts at: 4193 4193 Size: 4676 SfcI C'TryA_G Cuts at: 33 377 417 510 626 1719 2404 3397 3588 Size: 344 40 93 116 1093 685 993 191 Cuts at: 3588 33 Size: 1121 Fragments arranged by size: 1121 1093 993 685 344 191 116 93 40 SfoI GGC'GCC Cuts at: 2108 2356 2108 Size: 248 4428 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4676 SgrAI Cr'CCGG_yG Cuts at: 1093 1093 Size: 4676 SmaI CCC'GGG Cuts at: 1660 1698 1660 Size: 38 4638 SmlI C'TyrA_G Cuts at: 1831 2746 3238 3500 1831 Size: 915 492 262 3007 Fragments arranged by size: 3007 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4676 SphI G_CATG'C Cuts at: 1251 1729 2229 1251 Size: 478 500 3698 Fragments arranged by size: 3698 500 478 StuI AGG'CCT Cuts at: 452 792 452 Size: 340 4336 StyI C'CwwG_G Cuts at: 182 1506 1810 2164 182 Size: 1324 304 354 2694 Fragments arranged by size: 2694 1324 354 304 SwaI ATTT'AAAT Cuts at: 135 135 Size: 4676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2259 2392 2851 3060 2259 Size: 133 459 209 3875 Fragments arranged by size: 3875 459 209 133 TatI w'GTAC_w Cuts at: 4191 4191 Size: 4676 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4193 4225 4193 Size: 32 4644 Tth111I GACn'n_nGTC Cuts at: 439 966 2473 439 Size: 527 1507 2642 Fragments arranged by size: 2642 1507 527 XbaI T'CTAG_A Cuts at: 1707 1707 Size: 4676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2171 2171 Size: 4676 XmaI C'CCGG_G Cuts at: 1658 1696 1658 Size: 38 4638 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI XhoI XmnI ZraI