Restriction map for
pF1KB5986
(Circular) (Six-base) MAPSORT of: pF1KB5986.seq Check: 6265 from: 1 to: 4820
pF1KB5986 4820 bp
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 821 1303 1858 821
Size: 482 555 3783
Fragments arranged by size:
3783 555 482
Acc65I G'GTAC_C
Cuts at: 1712 1836 1712
Size: 124 4696
AclI AA'CG_TT
Cuts at: 4660 4660
Size: 4820
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1473 1532 2645 3077 3824 4092 1229
Size: 244 59 1113 432 747 268 1957
Fragments arranged by size:
1957 1113 747 432 268 244 59
AflIII A'CryG_T
Cuts at: 2172 3276 4253 2172
Size: 1104 977 2739
Fragments arranged by size:
2739 1104 977
AgeI A'CCGG_T
Cuts at: 3208 3208
Size: 4820
AleI CACnn'nnGTG
Cuts at: 629 629
Size: 4820
AlwNI CAG_nnn'CTG
Cuts at: 1761 2125 3692 1761
Size: 364 1567 2889
Fragments arranged by size:
2889 1567 364
ApaLI G'TGCA_C
Cuts at: 3590 3590
Size: 4820
ApoI r'AATT_y
Cuts at: 405 442 748 1618 1824 4173 4429 405
Size: 37 306 870 206 2349 256 796
Fragments arranged by size:
2349 870 796 306 256 206 37
AseI AT'TA_AT
Cuts at: 20 1427 20
Size: 1407 3413
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
AvaI C'yCGr_G
Cuts at: 737 1810 1840 737
Size: 1073 30 3717
Fragments arranged by size:
3717 1073 30
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 287 320 287
Size: 33 4787
BamHI G'GATC_C
Cuts at: 829 1845 829
Size: 1016 3804
BanI G'GyrC_C
Cuts at: 1712 1836 2250 2498 2533 1712
Size: 124 414 248 35 3999
Fragments arranged by size:
3999 414 248 124 35
BanII G_rGCy'C
Cuts at: 799 1834 2864 799
Size: 1035 1030 2755
Fragments arranged by size:
2755 1035 1030
BbeI G_GCGC'C
Cuts at: 2254 2502 2254
Size: 248 4572
BbsI GAAGACnn'nnnn_
Cuts at: 941 1415 1458 941
Size: 474 43 4303
Fragments arranged by size:
4303 474 43
BciVI GTATCCnnnnn_n'
Cuts at: 1289 2718 3479 4820 1289
Size: 1429 761 1341 1289
Fragments arranged by size:
1429 1341 1289 761
BclI T'GATC_A
Cuts at: 2340 2340
Size: 4820
BfrBI ATG'CAT
Cuts at: 700 700
Size: 4820
BglII A'GATC_T
Cuts at: 2335 4372 2335
Size: 2037 2783
BlpI GC'TnA_GC
Cuts at: 1932 1932
Size: 4820
Bme1580I G_kGCm'C
Cuts at: 535 2445 2538 3594 535
Size: 1910 93 1056 1761
Fragments arranged by size:
1910 1761 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 916 2032 2113 2448 4592 916
Size: 1116 81 335 2144 1144
Fragments arranged by size:
2144 1144 1116 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3187 3187
Size: 4820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 306 1597 181
Size: 125 1291 3404
Fragments arranged by size:
3404 1291 125
Bpu10I CC'TnA_GC
Cuts at: 365 365
Size: 4820
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1591 1996 2875 3367 3665 1591
Size: 405 879 492 298 2746
Fragments arranged by size:
2746 879 492 405 298
BsaI GGTCTCn'nnnn_
Cuts at: 4524 4524
Size: 4820
BsaAI yAC'GTr
Cuts at: 1803 2173 1803
Size: 370 4450
BsaBI GATnn'nnATC
Cuts at: 6 2334 4371 6
Size: 2328 2037 455
Fragments arranged by size:
2328 2037 455
BsaHI Gr'CG_yC
Cuts at: 2251 2499 3201 4697 2251
Size: 248 702 1496 2374
Fragments arranged by size:
2374 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2009 2530 3208 3482 3629 4040 2009
Size: 521 678 274 147 411 2789
Fragments arranged by size:
2789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 512 542 512
Size: 30 4790
BseYI C'CCAG_C
Cuts at: 798 1497 2245 3003 3580 4076 798
Size: 699 748 758 577 496 1542
Fragments arranged by size:
1542 758 748 699 577 496
BsiEI CG_ry'CG
Cuts at: 104 2362 2408 3616 104
Size: 2258 46 1208 1308
Fragments arranged by size:
2258 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1834 2612 2802 3594 1834
Size: 778 190 792 3060
Fragments arranged by size:
3060 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 509 509
Size: 4820
Bsp1286I G_dGCh'C
Cuts at: 535 799 1834 2445 2538 2612 2802 2864 3594
Size: 264 1035 611 93 74 190 62 730
Cuts at: 3594 535
Size: 1761
Fragments arranged by size:
1761 1035 730 611 264 190 93 74
62
BspEI T'CCGG_A
Cuts at: 4040 4040
Size: 4820
BspHI T'CATG_A
Cuts at: 3996 3996
Size: 4820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1870 2084 2386 2767 1870
Size: 214 302 381 3923
Fragments arranged by size:
3923 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3112 3166 4650 4815 50
Size: 3062 54 1484 165 55
Fragments arranged by size:
3062 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 875 2732 875
Size: 1857 2963
BsrFI r'CCGG_y
Cuts at: 859 2818 2999 3208 859
Size: 1959 181 209 2471
Fragments arranged by size:
2471 1959 209 181
BssSI C'ACGA_G
Cuts at: 3091 3449 3091
Size: 358 4462
BstAPI GCAn_nnn'nTGC
Cuts at: 2197 2197
Size: 4820
BstBI TT'CG_AA
Cuts at: 3181 3181
Size: 4820
BstEII G'GTnAC_C
Cuts at: 1736 1736
Size: 4820
BstYI r'GATC_y
Cuts at: 1 829 1845 2312 2335 2670 2916 3928 4014
Size: 828 1016 467 23 335 246 1012 86
Cuts at: 4014 4026 4372 1
Size: 12 346 449
Fragments arranged by size:
1016 1012 828 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1343 1343
Size: 4820
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2770 2951 3114 2770
Size: 181 163 4476
Fragments arranged by size:
4476 181 163
BtsI GCAGTG_nn'
Cuts at: 2063 2063
Size: 4820
ClaI AT'CG_AT
Cuts at: 1612 1612
Size: 4820
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 428 463 428
Size: 35 4785
DraI TTT'AAA
Cuts at: 552 1332 1819 552
Size: 780 487 3553
Fragments arranged by size:
3553 780 487
DrdI GACnn_nn'nnGTC
Cuts at: 2526 3384 2526
Size: 858 3962
EaeI y'GGCC_r
Cuts at: 861 2405 2579 2970 2997 4342 861
Size: 1544 174 391 27 1345 1339
Fragments arranged by size:
1544 1345 1339 391 174 27
EagI C'GGCC_G
Cuts at: 2405 2405
Size: 4820
EarI CTCTTCn'nnn_
Cuts at: 1508 2843 3053 1508
Size: 1335 210 3275
Fragments arranged by size:
3275 1335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3028 3338 3484 4630 3028
Size: 310 146 1146 3218
Fragments arranged by size:
3218 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1832 1832
Size: 4820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 306 1229 1473 1532 1597 2645 3077 3824
Size: 125 923 244 59 65 1048 432 747
Cuts at: 3824 4092 181
Size: 268 909
Fragments arranged by size:
1048 923 909 747 432 268 244 125
65 59
EcoO109I rG'GnC_Cy
Cuts at: 1959 1959
Size: 4820
EcoRI G'AATT_C
Cuts at: 1824 1824
Size: 4820
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 364 2839 364
Size: 2475 2345
FspI TGC'GCA
Cuts at: 2601 2601
Size: 4820
HaeII r_GCGC'y
Cuts at: 2254 2502 3524 4503 2254
Size: 248 1022 979 2571
Fragments arranged by size:
2571 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 141 173 814 846 1445 1477 2662 2692 2694
Size: 32 641 32 599 32 1185 30 2
Cuts at: 2694 2724 141
Size: 30 2237
Fragments arranged by size:
2237 1185 641 599 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1142 1859 1142
Size: 717 4103
HindIII A'AGCT_T
Cuts at: 1241 1241
Size: 4820
KasI G'GCGC_C
Cuts at: 2250 2498 2250
Size: 248 4572
KpnI G_GTAC'C
Cuts at: 1716 1840 1716
Size: 124 4696
MfeI C'AATT_G
Cuts at: 841 841
Size: 4820
MluI A'CGCG_T
Cuts at: 4253 4253
Size: 4820
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3491 3675 3491
Size: 184 4636
MscI TGG'CCA
Cuts at: 2581 2581
Size: 4820
MslI CAynn'nnrTG
Cuts at: 112 629 655 988 1153 2809 2936 112
Size: 517 26 333 165 1656 127 1996
Fragments arranged by size:
1996 1656 517 333 165 127 26
MspA1I CmG'CkG
Cuts at: 1493 1649 1931 2245 2605 3618 3863 1493
Size: 156 282 314 360 1013 245 2450
Fragments arranged by size:
2450 1013 360 314 282 245 156
NaeI GCC'GGC
Cuts at: 3001 3001
Size: 4820
NarI GG'CG_CC
Cuts at: 2251 2499 2251
Size: 248 4572
NgoMIV G'CCGG_C
Cuts at: 2999 2999
Size: 4820
NsiI A_TGCA'T
Cuts at: 702 702
Size: 4820
NspI r_CATG'y
Cuts at: 1722 1873 2373 3280 1722
Size: 151 500 907 3262
Fragments arranged by size:
3262 907 500 151
PciI A'CATG_T
Cuts at: 3276 3276
Size: 4820
PfoI T'CCnGG_A
Cuts at: 322 322
Size: 4820
PmeI GTTT'AAAC
Cuts at: 1819 1819
Size: 4820
PmlI CAC'GTG
Cuts at: 1803 1803
Size: 4820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3959 3991 3959
Size: 32 4788
PshAI GACnn'nnGTC
Cuts at: 1677 1677
Size: 4820
PsiI TTA'TAA
Cuts at: 473 1025 473
Size: 552 4268
PstI C_TGCA'G
Cuts at: 1867 2552 1867
Size: 685 4135
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4820
PvuII CAG'CTG
Cuts at: 1493 2245 2605 1493
Size: 752 360 3708
Fragments arranged by size:
3708 752 360
RsrII CG'GwC_CG
Cuts at: 3015 3015
Size: 4820
SacI G_AGCT'C
Cuts at: 1834 1834
Size: 4820
SalI G'TCGA_C
Cuts at: 1857 1857
Size: 4820
SapI GCTCTTCn'nnn_
Cuts at: 1508 2843 3053 1508
Size: 1335 210 3275
Fragments arranged by size:
3275 1335 210
SbfI CC_TGCA'GG
Cuts at: 1867 1867
Size: 4820
ScaI AGT'ACT
Cuts at: 4337 4337
Size: 4820
SfcI C'TryA_G
Cuts at: 33 308 1863 2548 3541 3732 33
Size: 275 1555 685 993 191 1121
Fragments arranged by size:
1555 1121 993 685 275 191
SfoI GGC'GCC
Cuts at: 2252 2500 2252
Size: 248 4572
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4820
SmaI CCC'GGG
Cuts at: 739 1842 739
Size: 1103 3717
SmlI C'TyrA_G
Cuts at: 1570 1975 2890 3382 3644 1570
Size: 405 915 492 262 2746
Fragments arranged by size:
2746 915 492 405 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4820
SphI G_CATG'C
Cuts at: 1873 2373 1873
Size: 500 4320
StuI AGG'CCT
Cuts at: 783 783
Size: 4820
StyI C'CwwG_G
Cuts at: 1954 2308 1954
Size: 354 4466
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1489 2403 2536 2995 3204 1489
Size: 914 133 459 209 3105
Fragments arranged by size:
3105 914 459 209 133
TatI w'GTAC_w
Cuts at: 4335 4335
Size: 4820
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 512 544 1345 1377 4337 4369 512
Size: 32 801 32 2960 32 963
Fragments arranged by size:
2960 963 801 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2617 2617
Size: 4820
XbaI T'CTAG_A
Cuts at: 759 1851 759
Size: 1092 3728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2315 2315
Size: 4820
XmaI C'CCGG_G
Cuts at: 737 1840 737
Size: 1103 3717
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BglI BmgBI BmtI BseRI
BsgI BsiWI BsmI BsrGI BssHII BstXI BstZ17I BtgI
DraIII EcoNI EcoRV FseI FspAI HpaI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI