Restriction map for
pF1KB5994
(Circular) (Six-base) MAPSORT of: pF1KB5994.seq Check: 9637 from: 1 to: 4940
pF1KB5994 4940 bp
With 182 enzymes: SgfI *
July 10, 2012 13:28 ..
AccI GT'mk_AC
Cuts at: 1978 1978
Size: 4940
Acc65I G'GTAC_C
Cuts at: 1956 1956
Size: 4940
AclI AA'CG_TT
Cuts at: 1696 4780 1696
Size: 3084 1856
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1024 1523 2765 3197 3944 4212 256
Size: 768 499 1242 432 747 268 984
Fragments arranged by size:
1242 984 768 747 499 432 268
AflIII A'CryG_T
Cuts at: 334 2292 3396 4373 334
Size: 1958 1104 977 901
Fragments arranged by size:
1958 1104 977 901
AgeI A'CCGG_T
Cuts at: 3328 3328
Size: 4940
AleI CACnn'nnGTG
Cuts at: 191 191
Size: 4940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1734 1734
Size: 4940
AlwNI CAG_nnn'CTG
Cuts at: 2245 3812 2245
Size: 1567 3373
ApaLI G'TGCA_C
Cuts at: 549 3710 549
Size: 3161 1779
ApoI r'AATT_y
Cuts at: 231 322 793 1306 1335 1384 1944 4293 4549
Size: 91 471 513 29 49 560 2349 256
Cuts at: 4549 231
Size: 622
Fragments arranged by size:
2349 622 560 513 471 256 91 49
29
AseI AT'TA_AT
Cuts at: 20 20
Size: 4940
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
AvaI C'yCGr_G
Cuts at: 1960 1960
Size: 4940
AvrII C'CTAG_G
Cuts at: 1467 1467
Size: 4940
BamHI G'GATC_C
Cuts at: 1965 1965
Size: 4940
BanI G'GyrC_C
Cuts at: 1956 2370 2618 2653 1956
Size: 414 248 35 4243
Fragments arranged by size:
4243 414 248 35
BanII G_rGCy'C
Cuts at: 1954 2984 1954
Size: 1030 3910
BbeI G_GCGC'C
Cuts at: 2374 2622 2374
Size: 248 4692
BbsI GAAGACnn'nnnn_
Cuts at: 618 1146 1535 618
Size: 528 389 4023
Fragments arranged by size:
4023 528 389
BbvCI CC'TCA_GC
Cuts at: 1139 1139
Size: 4940
BciVI GTATCCnnnnn_n'
Cuts at: 2838 3599 4940 2838
Size: 761 1341 2838
Fragments arranged by size:
2838 1341 761
BclI T'GATC_A
Cuts at: 2460 2460
Size: 4940
BglII A'GATC_T
Cuts at: 888 924 2455 4492 888
Size: 36 1531 2037 1336
Fragments arranged by size:
2037 1531 1336 36
BlpI GC'TnA_GC
Cuts at: 2052 2052
Size: 4940
Bme1580I G_kGCm'C
Cuts at: 553 2565 2658 3714 553
Size: 2012 93 1056 1779
Fragments arranged by size:
2012 1779 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2152 2233 2568 4712 2152
Size: 81 335 2144 2380
Fragments arranged by size:
2380 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3307 3307
Size: 4940
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 978 978
Size: 4940
Bpu10I CC'TnA_GC
Cuts at: 835 1139 835
Size: 304 4636
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 757 1443 2116 2995 3487 3785 757
Size: 686 673 879 492 298 1912
Fragments arranged by size:
1912 879 686 673 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 522 1461 1867 4644 522
Size: 939 406 2777 818
Fragments arranged by size:
2777 939 818 406
BsaAI yAC'GTr
Cuts at: 2293 2293
Size: 4940
BsaBI GATnn'nnATC
Cuts at: 6 2454 4491 6
Size: 2448 2037 455
Fragments arranged by size:
2448 2037 455
BsaHI Gr'CG_yC
Cuts at: 2371 2619 3321 4817 2371
Size: 248 702 1496 2494
Fragments arranged by size:
2494 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2129 2650 3328 3602 3749 4160 2129
Size: 521 678 274 147 411 2909
Fragments arranged by size:
2909 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1903 1903
Size: 4940
BseYI C'CCAG_C
Cuts at: 2365 3123 3700 4196 2365
Size: 758 577 496 3109
Fragments arranged by size:
3109 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1544 1593 1745 1544
Size: 49 152 4739
Fragments arranged by size:
4739 152 49
BsiEI CG_ry'CG
Cuts at: 104 2482 2528 3736 104
Size: 2378 46 1208 1308
Fragments arranged by size:
2378 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 553 1954 2732 2922 3714 553
Size: 1401 778 190 792 1779
Fragments arranged by size:
1779 1401 792 778 190
BsmI GAATG_Cn'
Cuts at: 484 916 1015 1288 1398 1520 484
Size: 432 99 273 110 122 3904
Fragments arranged by size:
3904 432 273 122 110 99
Bsp1286I G_dGCh'C
Cuts at: 553 1954 2565 2658 2732 2922 2984 3714 553
Size: 1401 611 93 74 190 62 730 1779
Fragments arranged by size:
1779 1401 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4160 4160
Size: 4940
BspHI T'CATG_A
Cuts at: 561 1278 4116 561
Size: 717 2838 1385
Fragments arranged by size:
2838 1385 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1885 1990 2204 2506 2887 1885
Size: 105 214 302 381 3938
Fragments arranged by size:
3938 381 302 214 105
BsrBI CCG'CTC
Cuts at: 50 3232 3286 4770 4935 50
Size: 3182 54 1484 165 55
Fragments arranged by size:
3182 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1046 2852 1046
Size: 1806 3134
BsrFI r'CCGG_y
Cuts at: 2938 3119 3328 2938
Size: 181 209 4550
Fragments arranged by size:
4550 209 181
BssSI C'ACGA_G
Cuts at: 3211 3569 3211
Size: 358 4582
BstAPI GCAn_nnn'nTGC
Cuts at: 2317 2317
Size: 4940
BstBI TT'CG_AA
Cuts at: 3301 3301
Size: 4940
BstEII G'GTnAC_C
Cuts at: 117 117
Size: 4940
BstXI CCAn_nnnn'nTGG
Cuts at: 796 796
Size: 4940
BstYI r'GATC_y
Cuts at: 1 888 924 1272 1965 2432 2455 2790 3036
Size: 887 36 348 693 467 23 335 246
Cuts at: 3036 4048 4134 4146 4492 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 887 693 467 449 348 346 335
246 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 1926 1926
Size: 4940
BtgI C'CryG_G
Cuts at: 918 1541 918
Size: 623 4317
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2890 3071 3234 2890
Size: 181 163 4596
Fragments arranged by size:
4596 181 163
BtsI GCAGTG_nn'
Cuts at: 283 551 1041 2183 283
Size: 268 490 1142 3040
Fragments arranged by size:
3040 1142 490 268
DraI TTT'AAA
Cuts at: 213 1311 1939 213
Size: 1098 628 3214
Fragments arranged by size:
3214 1098 628
DrdI GACnn_nn'nnGTC
Cuts at: 2646 3504 2646
Size: 858 4082
EaeI y'GGCC_r
Cuts at: 2525 2699 3090 3117 4462 2525
Size: 174 391 27 1345 3003
Fragments arranged by size:
3003 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2525 2525
Size: 4940
EarI CTCTTCn'nnn_
Cuts at: 422 566 1319 2963 3173 422
Size: 144 753 1644 210 2189
Fragments arranged by size:
2189 1644 753 210 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 967 3148 3458 3604 4750 967
Size: 2181 310 146 1146 1157
Fragments arranged by size:
2181 1157 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1952 1952
Size: 4940
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 978 1024 1523 2765 3197 3944 4212 256
Size: 722 46 499 1242 432 747 268 984
Fragments arranged by size:
1242 984 747 722 499 432 268 46
EcoO109I rG'GnC_Cy
Cuts at: 2079 2079
Size: 4940
EcoRI G'AATT_C
Cuts at: 1944 1944
Size: 4940
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 635 1170 2959 635
Size: 535 1789 2616
Fragments arranged by size:
2616 1789 535
FspI TGC'GCA
Cuts at: 2721 2721
Size: 4940
HaeII r_GCGC'y
Cuts at: 2374 2622 3644 4623 2374
Size: 248 1022 979 2691
Fragments arranged by size:
2691 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 976 1008 1522 1554 2782 2812 2814 2844 976
Size: 32 514 32 1228 30 2 30 3072
Fragments arranged by size:
3072 1228 514 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1436 1979 1436
Size: 543 4397
KasI G'GCGC_C
Cuts at: 2370 2618 2370
Size: 248 4692
KpnI G_GTAC'C
Cuts at: 1960 1960
Size: 4940
MluI A'CGCG_T
Cuts at: 4373 4373
Size: 4940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1010 1465 3611 3795 1010
Size: 455 2146 184 2155
Fragments arranged by size:
2155 2146 455 184
MscI TGG'CCA
Cuts at: 2701 2701
Size: 4940
MslI CAynn'nnrTG
Cuts at: 191 497 566 2929 3056 191
Size: 306 69 2363 127 2075
Fragments arranged by size:
2363 2075 306 127 69
MspA1I CmG'CkG
Cuts at: 355 1267 2051 2365 2725 3738 3983 355
Size: 912 784 314 360 1013 245 1312
Fragments arranged by size:
1312 1013 912 784 360 314 245
NaeI GCC'GGC
Cuts at: 3121 3121
Size: 4940
NarI GG'CG_CC
Cuts at: 2371 2619 2371
Size: 248 4692
NcoI C'CATG_G
Cuts at: 918 918
Size: 4940
NdeI CA'TA_TG
Cuts at: 1236 1236
Size: 4940
NgoMIV G'CCGG_C
Cuts at: 3119 3119
Size: 4940
NspI r_CATG'y
Cuts at: 338 1167 1993 2493 3400 338
Size: 829 826 500 907 1878
Fragments arranged by size:
1878 907 829 826 500
PciI A'CATG_T
Cuts at: 334 3396 334
Size: 3062 1878
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4940
PmeI GTTT'AAAC
Cuts at: 1939 1939
Size: 4940
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 4079
Size: 32 4908
PstI C_TGCA'G
Cuts at: 1272 1987 2672 1272
Size: 715 685 3540
Fragments arranged by size:
3540 715 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4940
PvuII CAG'CTG
Cuts at: 355 2365 2725 355
Size: 2010 360 2570
Fragments arranged by size:
2570 2010 360
RsrII CG'GwC_CG
Cuts at: 3135 3135
Size: 4940
SacI G_AGCT'C
Cuts at: 1954 1954
Size: 4940
SalI G'TCGA_C
Cuts at: 1977 1977
Size: 4940
SapI GCTCTTCn'nnn_
Cuts at: 1319 2963 3173 1319
Size: 1644 210 3086
Fragments arranged by size:
3086 1644 210
SbfI CC_TGCA'GG
Cuts at: 1987 1987
Size: 4940
ScaI AGT'ACT
Cuts at: 4457 4457
Size: 4940
SfcI C'TryA_G
Cuts at: 33 1268 1983 2668 3661 3852 33
Size: 1235 715 685 993 191 1121
Fragments arranged by size:
1235 1121 993 715 685 191
SfoI GGC'GCC
Cuts at: 2372 2620 2372
Size: 248 4692
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4940
SmaI CCC'GGG
Cuts at: 1962 1962
Size: 4940
SmlI C'TyrA_G
Cuts at: 772 1458 2095 3010 3502 3764 772
Size: 686 637 915 492 262 1948
Fragments arranged by size:
1948 915 686 637 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4940
SphI G_CATG'C
Cuts at: 1993 2493 1993
Size: 500 4440
StyI C'CwwG_G
Cuts at: 242 918 1467 1619 1790 2074 2428 242
Size: 676 549 152 171 284 354 2754
Fragments arranged by size:
2754 676 549 354 284 171 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2523 2656 3115 3324 2523
Size: 133 459 209 4139
Fragments arranged by size:
4139 459 209 133
TatI w'GTAC_w
Cuts at: 1379 4455 1379
Size: 3076 1864
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4457 4489 4457
Size: 32 4908
Tth111I GACn'n_nGTC
Cuts at: 1594 2737 1594
Size: 1143 3797
XbaI T'CTAG_A
Cuts at: 1248 1971 1248
Size: 723 4217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2435 2435
Size: 4940
XmaI C'CCGG_G
Cuts at: 1960 1960
Size: 4940
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI BsiWI
BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI