Restriction map for
pF1KB5995
(Circular) (Six-base) MAPSORT of: pF1KB5995.seq Check: 140 from: 1 to: 4844
pF1KB5995 4844 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 251 1647 1882 251
Size: 1396 235 3213
Fragments arranged by size:
3213 1396 235
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 4684 4684
Size: 4844
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 430 580 2669 3101 3848 4116 212
Size: 218 150 2089 432 747 268 940
Fragments arranged by size:
2089 940 747 432 268 218 150
AflIII A'CryG_T
Cuts at: 587 2196 3300 4277 587
Size: 1609 1104 977 1154
Fragments arranged by size:
1609 1154 1104 977
AgeI A'CCGG_T
Cuts at: 3232 3232
Size: 4844
AhdI GACnn_n'nnGTC
Cuts at: 464 464
Size: 4844
AlwNI CAG_nnn'CTG
Cuts at: 895 907 1522 2149 3716 895
Size: 12 615 627 1567 2023
Fragments arranged by size:
2023 1567 627 615 12
ApaLI G'TGCA_C
Cuts at: 3614 3614
Size: 4844
ApoI r'AATT_y
Cuts at: 349 674 1848 4197 4453 349
Size: 325 1174 2349 256 740
Fragments arranged by size:
2349 1174 740 325 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4844
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 1061 1240 1328 1358 1448 1681 1864 1061
Size: 179 88 30 90 233 183 4041
Fragments arranged by size:
4041 233 183 179 90 88 30
BamHI G'GATC_C
Cuts at: 654 754 1033 1323 1597 1869 654
Size: 100 279 290 274 272 3629
Fragments arranged by size:
3629 290 279 274 272 100
BanI G'GyrC_C
Cuts at: 427 1860 2274 2522 2557 427
Size: 1433 414 248 35 2714
Fragments arranged by size:
2714 1433 414 248 35
BanII G_rGCy'C
Cuts at: 1247 1858 2888 1247
Size: 611 1030 3203
Fragments arranged by size:
3203 1030 611
BbeI G_GCGC'C
Cuts at: 2278 2526 2278
Size: 248 4596
BbvCI CC'TCA_GC
Cuts at: 722 722
Size: 4844
BciVI GTATCCnnnnn_n'
Cuts at: 1497 1804 2742 3503 4844 1497
Size: 307 938 761 1341 1497
Fragments arranged by size:
1497 1341 938 761 307
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BfrBI ATG'CAT
Cuts at: 819 819
Size: 4844
BglI GCCn_nnn'nGGC
Cuts at: 796 1369 1667 796
Size: 573 298 3973
Fragments arranged by size:
3973 573 298
BglII A'GATC_T
Cuts at: 2359 4396 2359
Size: 2037 2807
BlpI GC'TnA_GC
Cuts at: 1956 1956
Size: 4844
Bme1580I G_kGCm'C
Cuts at: 432 2469 2562 3618 432
Size: 2037 93 1056 1658
Fragments arranged by size:
2037 1658 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 795 1510 1556 1606 2056 2137 2472 4616 795
Size: 715 46 50 450 81 335 2144 1023
Fragments arranged by size:
2144 1023 715 450 335 81 50 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 4844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 950 1142 1850 950
Size: 192 708 3944
Fragments arranged by size:
3944 708 192
Bpu10I CC'TnA_GC
Cuts at: 722 750 921 947 1778 722
Size: 28 171 26 831 3788
Fragments arranged by size:
3788 831 171 28 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2020 2899 3391 3689 2020
Size: 879 492 298 3175
Fragments arranged by size:
3175 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 122 451 4548 122
Size: 329 4097 418
Fragments arranged by size:
4097 418 329
BsaAI yAC'GTr
Cuts at: 2197 2197
Size: 4844
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 2275 2523 3225 4721 2275
Size: 248 702 1496 2398
Fragments arranged by size:
2398 1496 702 248
BsaWI w'CCGG_w
Cuts at: 657 2033 2554 3232 3506 3653 4064 657
Size: 1376 521 678 274 147 411 1437
Fragments arranged by size:
1437 1376 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1823 1853 1823
Size: 30 4814
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 827 947 827
Size: 120 4724
BseYI C'CCAG_C
Cuts at: 132 739 2269 3027 3604 4100 132
Size: 607 1530 758 577 496 876
Fragments arranged by size:
1530 876 758 607 577 496
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3640 104
Size: 2282 46 1208 1308
Fragments arranged by size:
2282 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 366 1836 1858 2636 2826 3618 366
Size: 1470 22 778 190 792 1592
Fragments arranged by size:
1592 1470 792 778 190 22
BsmI GAATG_Cn'
Cuts at: 821 944 962 1556 1637 821
Size: 123 18 594 81 4028
Fragments arranged by size:
4028 594 123 81 18
Bsp1286I G_dGCh'C
Cuts at: 366 432 1247 1836 1858 2469 2562 2636 2826
Size: 66 815 589 22 611 93 74 190
Cuts at: 2826 2888 3618 366
Size: 62 730 1592
Fragments arranged by size:
1592 815 730 611 589 190 93 74
66 62 22
BspEI T'CCGG_A
Cuts at: 657 4064 657
Size: 3407 1437
BspHI T'CATG_A
Cuts at: 1710 4020 1710
Size: 2310 2534
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1725 1894 2108 2410 2791 1725
Size: 169 214 302 381 3778
Fragments arranged by size:
3778 381 302 214 169
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1415 1445 1475 2756 1415
Size: 30 30 1281 3503
Fragments arranged by size:
3503 1281 30 30
BsrFI r'CCGG_y
Cuts at: 2842 3023 3232 2842
Size: 181 209 4454
Fragments arranged by size:
4454 209 181
BssSI C'ACGA_G
Cuts at: 1343 1433 3115 3473 1343
Size: 90 1682 358 2714
Fragments arranged by size:
2714 1682 358 90
BstAPI GCAn_nnn'nTGC
Cuts at: 322 2221 322
Size: 1899 2945
BstBI TT'CG_AA
Cuts at: 3205 3205
Size: 4844
BstXI CCAn_nnnn'nTGG
Cuts at: 739 1186 1500 739
Size: 447 314 4083
Fragments arranged by size:
4083 447 314
BstYI r'GATC_y
Cuts at: 1 654 754 1033 1323 1597 1869 2336 2359
Size: 653 100 279 290 274 272 467 23
Cuts at: 2359 2694 2940 3952 4038 4050 4396 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 653 467 449 346 335 290 279
274 272 246 100 86 23 12
BstZ17I GTA'TAC
Cuts at: 252 1648 252
Size: 1396 3448
Bsu36I CC'TnA_GG
Cuts at: 470 785 794 470
Size: 315 9 4520
Fragments arranged by size:
4520 315 9
BtgI C'CryG_G
Cuts at: 107 732 980 1093 1364 107
Size: 625 248 113 271 3587
Fragments arranged by size:
3587 625 271 248 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 667 1438 1468 2794 2975 3138 667
Size: 771 30 1326 181 163 2373
Fragments arranged by size:
2373 1326 771 181 163 30
BtsI GCAGTG_nn'
Cuts at: 2087 2087
Size: 4844
DraI TTT'AAA
Cuts at: 1805 1843 1805
Size: 38 4806
DraIII CAC_nnn'GTG
Cuts at: 322 322
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 2550 3408 2550
Size: 858 3986
EaeI y'GGCC_r
Cuts at: 1188 2429 2603 2994 3021 4366 1188
Size: 1241 174 391 27 1345 1666
Fragments arranged by size:
1666 1345 1241 391 174 27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 188 398 406 2867 3077 188
Size: 210 8 2461 210 1955
Fragments arranged by size:
2461 1955 210 210 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3362 3508 4654 3052
Size: 310 146 1146 3242
Fragments arranged by size:
3242 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 430 580 950 1142 1850 2669 3101 3848
Size: 218 150 370 192 708 819 432 747
Cuts at: 3848 4116 212
Size: 268 940
Fragments arranged by size:
940 819 747 708 432 370 268 218
192 150
EcoNI CCTnn'n_nnAGG
Cuts at: 749 932 749
Size: 183 4661
EcoO109I rG'GnC_Cy
Cuts at: 789 904 1273 1534 1558 1608 1983 789
Size: 115 369 261 24 50 375 3650
Fragments arranged by size:
3650 375 369 261 115 50 24
EcoRI G'AATT_C
Cuts at: 349 1848 349
Size: 1499 3345
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 228 2863 228
Size: 2635 2209
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 2278 2526 3548 4527 2278
Size: 248 1022 979 2595
Fragments arranged by size:
2595 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 446 450 478 482 1416 1448 2686 2716 2718
Size: 4 28 4 934 32 1238 30 2
Cuts at: 2718 2748 446
Size: 30 2542
Fragments arranged by size:
2542 1238 934 32 30 30 28 4
4 2
HincII GTy'rAC
Cuts at: 1883 1883
Size: 4844
HindIII A'AGCT_T
Cuts at: 848 848
Size: 4844
KasI G'GCGC_C
Cuts at: 2274 2522 2274
Size: 248 4596
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 204 933 3515 3699 204
Size: 729 2582 184 1349
Fragments arranged by size:
2582 1349 729 184
MscI TGG'CCA
Cuts at: 1190 2605 1190
Size: 1415 3429
MslI CAynn'nnrTG
Cuts at: 824 2833 2960 824
Size: 2009 127 2708
Fragments arranged by size:
2708 2009 127
MspA1I CmG'CkG
Cuts at: 136 892 1029 1955 2269 2629 3642 3887 136
Size: 756 137 926 314 360 1013 245 1093
Fragments arranged by size:
1093 1013 926 756 360 314 245 137
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 2275 2523 2275
Size: 248 4596
NcoI C'CATG_G
Cuts at: 107 732 980 1364 107
Size: 625 248 384 3587
Fragments arranged by size:
3587 625 384 248
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NsiI A_TGCA'T
Cuts at: 821 821
Size: 4844
NspI r_CATG'y
Cuts at: 591 823 1897 2397 3304 591
Size: 232 1074 500 907 2131
Fragments arranged by size:
2131 1074 907 500 232
PasI CC'CwG_GG
Cuts at: 1593 1593
Size: 4844
PciI A'CATG_T
Cuts at: 587 3300 587
Size: 2713 2131
PflMI CCAn_nnn'nTGG
Cuts at: 733 807 967 1522 733
Size: 74 160 555 4055
Fragments arranged by size:
4055 555 160 74
PfoI T'CCnGG_A
Cuts at: 927 927
Size: 4844
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4812
PpuMI rG'GwC_Cy
Cuts at: 904 1273 1534 1558 1608 904
Size: 369 261 24 50 4140
Fragments arranged by size:
4140 369 261 50 24
PshAI GACnn'nnGTC
Cuts at: 975 975
Size: 4844
PspXI vC'TCGA_Gb
Cuts at: 1061 1061
Size: 4844
PstI C_TGCA'G
Cuts at: 1755 1891 2576 1755
Size: 136 685 4023
Fragments arranged by size:
4023 685 136
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 892 2269 2629 892
Size: 1377 360 3107
Fragments arranged by size:
3107 1377 360
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SanDI GG'GwC_CC
Cuts at: 1558 1558
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 188 398 406 2867 3077 188
Size: 210 8 2461 210 1955
Fragments arranged by size:
2461 1955 210 210 8
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SfcI C'TryA_G
Cuts at: 33 1751 1887 2572 3565 3756 33
Size: 1718 136 685 993 191 1121
Fragments arranged by size:
1718 1121 993 685 191 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 796 796
Size: 4844
SfoI GGC'GCC
Cuts at: 2276 2524 2276
Size: 248 4596
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1683 1866 1683
Size: 183 4661
SmlI C'TyrA_G
Cuts at: 1061 1999 2914 3406 3668 1061
Size: 938 915 492 262 2237
Fragments arranged by size:
2237 938 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
StyI C'CwwG_G
Cuts at: 107 417 732 980 1301 1364 1978 2332 107
Size: 310 315 248 321 63 614 354 2619
Fragments arranged by size:
2619 614 354 321 315 310 248 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 799 1185 2427 2560 3019 3228 799
Size: 386 1242 133 459 209 2415
Fragments arranged by size:
2415 1242 459 386 209 133
TatI w'GTAC_w
Cuts at: 4359 4359
Size: 4844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 746 778 4361 4393 746
Size: 32 3583 32 1197
Fragments arranged by size:
3583 1197 32 32
Tth111I GACn'n_nGTC
Cuts at: 2641 2641
Size: 4844
XbaI T'CTAG_A
Cuts at: 1875 1875
Size: 4844
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 808 1254 2339 808
Size: 446 1085 3313
Fragments arranged by size:
3313 1085 446
XhoI C'TCGA_G
Cuts at: 1061 1061
Size: 4844
XmaI C'CCGG_G
Cuts at: 1681 1864 1681
Size: 183 4661
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PspXI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsgI
BsiWI BsmBI BsrGI BssHII BstEII ClaI CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
PacI PmlI PsiI PspOMI PsrI SacII SexAI SgrAI
SnaBI SrfI SspI StuI SwaI XmnI ZraI