Restriction map for
pF1KB6003
(Circular) (Six-base) MAPSORT of: pF1KB6003.seq Check: 5235 from: 1 to: 6707
pF1KB6003 6707 bp
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 255 255
Size: 6707
AatII G_ACGT'C
Cuts at: 3319 3319
Size: 6707
AccI GT'mk_AC
Cuts at: 615 3560 3745 615
Size: 2945 185 3577
Fragments arranged by size:
3577 2945 185
Acc65I G'GTAC_C
Cuts at: 3723 3723
Size: 6707
AclI AA'CG_TT
Cuts at: 917 6547 917
Size: 5630 1077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 551 1761 2017 2233 2314 2459 2728 3604 3700
Size: 1210 256 216 81 145 269 876 96
Cuts at: 3700 4532 4964 5317 5979 551
Size: 832 432 353 662 1279
Fragments arranged by size:
1279 1210 876 832 662 432 353 269
256 216 145 96 81
AflII C'TTAA_G
Cuts at: 1278 1278
Size: 6707
AflIII A'CryG_T
Cuts at: 2808 4059 5859 6140 2808
Size: 1251 1800 281 3375
Fragments arranged by size:
3375 1800 1251 281
AleI CACnn'nnGTG
Cuts at: 3594 3594
Size: 6707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3402 3402
Size: 6707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3301 3333 3301
Size: 32 6675
AlwNI CAG_nnn'CTG
Cuts at: 2427 2967 3402 4012 5450 2427
Size: 540 435 610 1438 3684
Fragments arranged by size:
3684 1438 610 540 435
ApaLI G'TGCA_C
Cuts at: 2657 5545 2657
Size: 2888 3819
ApoI r'AATT_y
Cuts at: 377 593 1868 2046 2493 2571 2620 3577 3711
Size: 216 1275 178 447 78 49 957 134
Cuts at: 3711 6060 6316 377
Size: 2349 256 768
Fragments arranged by size:
2349 1275 957 768 447 256 216 178
134 78 49
AseI AT'TA_AT
Cuts at: 20 313 1886 1940 20
Size: 293 1573 54 4787
Fragments arranged by size:
4787 1573 293 54
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
AvaI C'yCGr_G
Cuts at: 3625 3727 3625
Size: 102 6605
BamHI G'GATC_C
Cuts at: 1703 3732 1703
Size: 2029 4678
BanI G'GyrC_C
Cuts at: 462 3280 3723 4137 4385 4420 462
Size: 2818 443 414 248 35 2749
Fragments arranged by size:
2818 2749 443 414 248 35
BanII G_rGCy'C
Cuts at: 2450 3721 4751 2450
Size: 1271 1030 4406
Fragments arranged by size:
4406 1271 1030
BbeI G_GCGC'C
Cuts at: 4141 4389 4141
Size: 248 6459
BbsI GAAGACnn'nnnn_
Cuts at: 443 692 1520 2023 443
Size: 249 828 503 5127
Fragments arranged by size:
5127 828 503 249
BbvCI CC'TCA_GC
Cuts at: 175 175
Size: 6707
BciVI GTATCCnnnnn_n'
Cuts at: 4605 5661 6707 4605
Size: 1056 1046 4605
Fragments arranged by size:
4605 1056 1046
BclI T'GATC_A
Cuts at: 4227 4227
Size: 6707
BfrBI ATG'CAT
Cuts at: 1579 3150 1579
Size: 1571 5136
BglI GCCn_nnn'nGGC
Cuts at: 112 3505 112
Size: 3393 3314
BglII A'GATC_T
Cuts at: 1165 4222 6259 1165
Size: 3057 2037 1613
Fragments arranged by size:
3057 2037 1613
BlpI GC'TnA_GC
Cuts at: 1157 3819 1157
Size: 2662 4045
Bme1580I G_kGCm'C
Cuts at: 467 2232 2661 3437 4332 4425 5549 467
Size: 1765 429 776 895 93 1124 1625
Fragments arranged by size:
1765 1625 1124 895 776 429 93
BmrI ACTGGGnnnn_n'
Cuts at: 240 3919 4000 4335 6479 240
Size: 3679 81 335 2144 468
Fragments arranged by size:
3679 2144 468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5074 5074
Size: 6707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 834 1156 1159 1446 2013 3315 3372 548
Size: 286 322 3 287 567 1302 57 3883
Fragments arranged by size:
3883 1302 567 322 287 286 57 3
Bpu10I CC'TnA_GC
Cuts at: 175 175
Size: 6707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 654 2254 3883 4762 5476 5774 654
Size: 1600 1629 879 714 298 1587
Fragments arranged by size:
1629 1600 1587 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 6411 6411
Size: 6707
BsaAI yAC'GTr
Cuts at: 3564 4060 3564
Size: 496 6211
BsaBI GATnn'nnATC
Cuts at: 6 4221 6258 6
Size: 4215 2037 455
Fragments arranged by size:
4215 2037 455
BsaHI Gr'CG_yC
Cuts at: 2679 3316 3485 4138 4386 5088 6584 2679
Size: 637 169 653 248 702 1496 2802
Fragments arranged by size:
2802 1496 702 653 637 248 169
BsaWI w'CCGG_w
Cuts at: 1683 3896 4417 5095 5506 5653 5927 1683
Size: 2213 521 678 411 147 274 2463
Fragments arranged by size:
2463 2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 537 567 3199 3229 537
Size: 30 2632 30 4015
Fragments arranged by size:
4015 2632 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 1282 2482 2557 163
Size: 1119 1200 75 4313
Fragments arranged by size:
4313 1200 1119 75
BseYI C'CCAG_C
Cuts at: 2977 3005 3636 4132 4890 5555 5963 2977
Size: 28 631 496 758 665 408 3721
Fragments arranged by size:
3721 758 665 631 496 408 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 650 3178 3388 650
Size: 2528 210 3969
Fragments arranged by size:
3969 2528 210
BsiEI CG_ry'CG
Cuts at: 104 4249 4295 5525 104
Size: 4145 46 1230 1286
Fragments arranged by size:
4145 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 864 933 1055 2661 3721 4499 4689 5549 864
Size: 69 122 1606 1060 778 190 860 2022
Fragments arranged by size:
2022 1606 1060 860 778 190 122 69
BsmI GAATG_Cn'
Cuts at: 2995 2995
Size: 6707
Bsp1286I G_dGCh'C
Cuts at: 467 864 933 1055 2232 2450 2661 3437 3721
Size: 397 69 122 1177 218 211 776 284
Cuts at: 3721 4332 4425 4499 4689 4751 5549 467
Size: 611 93 74 190 62 798 1625
Fragments arranged by size:
1625 1177 798 776 611 397 284 218
211 190 122 93 74 69 62
BspEI T'CCGG_A
Cuts at: 1683 1683
Size: 6707
BspHI T'CATG_A
Cuts at: 297 753 1293 2367 5139 297
Size: 456 540 1074 2772 1865
Fragments arranged by size:
2772 1865 1074 540 456
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 3757 3971 4273 4654 255
Size: 3502 214 302 381 2308
Fragments arranged by size:
3502 2308 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4999 5053 6537 6702 50
Size: 4949 54 1484 165 55
Fragments arranged by size:
4949 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 893 1031 1371 2556 3116 3132 3561 3575 4619
Size: 138 340 1185 560 16 429 14 1044
Cuts at: 4619 893
Size: 2981
Fragments arranged by size:
2981 1185 1044 560 429 340 138 16
14
BsrFI r'CCGG_y
Cuts at: 134 4705 4886 134
Size: 4571 181 1955
Fragments arranged by size:
4571 1955 181
BsrGI T'GTAC_A
Cuts at: 642 1624 2330 642
Size: 982 706 5019
Fragments arranged by size:
5019 982 706
BssSI C'ACGA_G
Cuts at: 206 780 3141 4978 5686 206
Size: 574 2361 1837 708 1227
Fragments arranged by size:
2361 1837 1227 708 574
BstAPI GCAn_nnn'nTGC
Cuts at: 4084 4084
Size: 6707
BstBI TT'CG_AA
Cuts at: 5068 5068
Size: 6707
BstEII G'GTnAC_C
Cuts at: 742 742
Size: 6707
BstXI CCAn_nnnn'nTGG
Cuts at: 3169 3300 3169
Size: 131 6576
BstYI r'GATC_y
Cuts at: 1 480 1165 1703 1830 3732 4199 4222 4557
Size: 479 685 538 127 1902 467 23 335
Cuts at: 4557 4803 5109 5121 5207 6259 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1902 1052 685 538 479 467 449 335
306 246 127 86 23 12
BstZ17I GTA'TAC
Cuts at: 616 616
Size: 6707
Bsu36I CC'TnA_GG
Cuts at: 468 468
Size: 6707
BtgI C'CryG_G
Cuts at: 107 3503 107
Size: 3396 3311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4657 4838 5001 4657
Size: 181 163 6363
Fragments arranged by size:
6363 181 163
BtsI GCAGTG_nn'
Cuts at: 1725 2361 3950 1725
Size: 636 1589 4482
Fragments arranged by size:
4482 1589 636
DraI TTT'AAA
Cuts at: 3706 3706
Size: 6707
DraIII CAC_nnn'GTG
Cuts at: 1050 1050
Size: 6707
DrdI GACnn_nn'nnGTC
Cuts at: 4413 5757 4413
Size: 1344 5363
EaeI y'GGCC_r
Cuts at: 132 2090 2153 2732 3449 4292 4466 4857 4884
Size: 1958 63 579 717 843 174 391 27
Cuts at: 4884 6229 132
Size: 1345 610
Fragments arranged by size:
1958 1345 843 717 610 579 391 174
63 27
EagI C'GGCC_G
Cuts at: 4292 4292
Size: 6707
EarI CTCTTCn'nnn_
Cuts at: 383 879 1736 2438 4730 4940 383
Size: 496 857 702 2292 210 2150
Fragments arranged by size:
2292 2150 857 702 496 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 174 3382 4915 5657 5803 6517 174
Size: 3208 1533 742 146 714 364
Fragments arranged by size:
3208 1533 742 714 364 146
EcoICRI GAG'CTC
Cuts at: 3719 3719
Size: 6707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 548 551 834 1156 1159 1446 1761 2013 2017
Size: 3 283 322 3 287 315 252 4
Cuts at: 2017 2233 2314 2459 2728 3315 3372 3604 3700
Size: 216 81 145 269 587 57 232 96
Cuts at: 3700 4532 4964 5317 5979 548
Size: 832 432 353 662 1276
Fragments arranged by size:
1276 832 662 587 432 353 322 315
287 283 269 252 232 216 145 96
81 57 4 3 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 3394 2642
Size: 752 5955
EcoO109I rG'GnC_Cy
Cuts at: 212 474 2472 2716 3324 3846 212
Size: 262 1998 244 608 522 3073
Fragments arranged by size:
3073 1998 608 522 262 244
EcoRI G'AATT_C
Cuts at: 2493 2571 3711 2493
Size: 78 1140 5489
Fragments arranged by size:
5489 1140 78
EcoRV GAT'ATC
Cuts at: 1413 1509 1413
Size: 96 6611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 483 4726 483
Size: 4243 2464
FspI TGC'GCA
Cuts at: 4488 4488
Size: 6707
HaeII r_GCGC'y
Cuts at: 3696 4141 4389 5619 6390 3696
Size: 445 248 1230 771 4013
Fragments arranged by size:
4013 1230 771 445 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 535 567 642 674 1438 1470 1477 1509 1688
Size: 32 75 32 764 32 7 32 179
Cuts at: 1688 1720 4549 4579 4581 4611 535
Size: 32 2829 30 2 30 2631
Fragments arranged by size:
2829 2631 764 179 75 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 336 906 1353 2085 3218 3746 336
Size: 570 447 732 1133 528 3297
Fragments arranged by size:
3297 1133 732 570 528 447
HindIII A'AGCT_T
Cuts at: 2147 3616 2147
Size: 1469 5238
HpaI GTT'AAC
Cuts at: 336 3218 336
Size: 2882 3825
KasI G'GCGC_C
Cuts at: 4137 4385 4137
Size: 248 6459
KpnI G_GTAC'C
Cuts at: 3727 3727
Size: 6707
MluI A'CGCG_T
Cuts at: 6140 6140
Size: 6707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3203 5466 5650 3203
Size: 2263 184 4260
Fragments arranged by size:
4260 2263 184
MscI TGG'CCA
Cuts at: 2092 2155 2734 3451 4468 2092
Size: 63 579 717 1017 4331
Fragments arranged by size:
4331 1017 717 579 63
MslI CAynn'nnrTG
Cuts at: 296 313 2123 3145 3167 3594 4696 4823 296
Size: 17 1810 1022 22 427 1102 127 2180
Fragments arranged by size:
2180 1810 1102 1022 427 127 22 17
MspA1I CmG'CkG
Cuts at: 2306 3005 3818 4132 4492 5276 5521 2306
Size: 699 813 314 360 784 245 3492
Fragments arranged by size:
3492 813 784 699 360 314 245
NaeI GCC'GGC
Cuts at: 4888 4888
Size: 6707
NarI GG'CG_CC
Cuts at: 4138 4386 4138
Size: 248 6459
NcoI C'CATG_G
Cuts at: 107 3503 107
Size: 3396 3311
NgoMIV G'CCGG_C
Cuts at: 4886 4886
Size: 6707
NsiI A_TGCA'T
Cuts at: 1581 3152 1581
Size: 1571 5136
NspI r_CATG'y
Cuts at: 622 1117 3760 4260 5863 622
Size: 495 2643 500 1603 1466
Fragments arranged by size:
2643 1603 1466 500 495
PciI A'CATG_T
Cuts at: 5859 5859
Size: 6707
PflMI CCAn_nnn'nTGG
Cuts at: 230 2967 3476 230
Size: 2737 509 3461
Fragments arranged by size:
3461 2737 509
PmeI GTTT'AAAC
Cuts at: 3706 3706
Size: 6707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 763 795 2538 2570 5153 5185 763
Size: 32 1743 32 2583 32 2285
Fragments arranged by size:
2583 2285 1743 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 474 2472 2716 3324 474
Size: 1998 244 608 3857
Fragments arranged by size:
3857 1998 608 244
PshAI GACnn'nnGTC
Cuts at: 524 740 524
Size: 216 6491
PstI C_TGCA'G
Cuts at: 2761 3754 4439 2761
Size: 993 685 5029
Fragments arranged by size:
5029 993 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6707
PvuII CAG'CTG
Cuts at: 4132 4492 4132
Size: 360 6347
RsrII CG'GwC_CG
Cuts at: 4902 4902
Size: 6707
SacI G_AGCT'C
Cuts at: 3721 3721
Size: 6707
SalI G'TCGA_C
Cuts at: 3744 3744
Size: 6707
SapI GCTCTTCn'nnn_
Cuts at: 2438 4730 4940 2438
Size: 2292 210 4205
Fragments arranged by size:
4205 2292 210
SbfI CC_TGCA'GG
Cuts at: 3754 3754
Size: 6707
ScaI AGT'ACT
Cuts at: 6224 6224
Size: 6707
SfcI C'TryA_G
Cuts at: 33 763 1733 2757 3750 4435 5403 5594 33
Size: 730 970 1024 993 685 968 191 1146
Fragments arranged by size:
1146 1024 993 970 968 730 685 191
SfoI GGC'GCC
Cuts at: 4139 4387 4139
Size: 248 6459
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6707
SmaI CCC'GGG
Cuts at: 3729 3729
Size: 6707
SmlI C'TyrA_G
Cuts at: 633 1278 2233 3862 4777 5491 5753 633
Size: 645 955 1629 915 714 262 1587
Fragments arranged by size:
1629 1587 955 915 714 645 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6707
SphI G_CATG'C
Cuts at: 1117 3760 4260 1117
Size: 2643 500 3564
Fragments arranged by size:
3564 2643 500
SspI AAT'ATT
Cuts at: 2407 2407
Size: 6707
StuI AGG'CCT
Cuts at: 761 2239 2251 761
Size: 1478 12 5217
Fragments arranged by size:
5217 1478 12
StyI C'CwwG_G
Cuts at: 107 458 2918 3366 3503 3841 4195 107
Size: 351 2460 448 137 338 354 2619
Fragments arranged by size:
2619 2460 448 354 351 338 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1509 4290 4423 4882 5091 1509
Size: 2781 133 459 209 3125
Fragments arranged by size:
3125 2781 459 209 133
TatI w'GTAC_w
Cuts at: 586 642 1624 2330 6222 586
Size: 56 982 706 3892 1071
Fragments arranged by size:
3892 1071 982 706 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 537 569 859 891 1780 1812 2256 2288 2687
Size: 32 290 32 889 32 444 32 399
Cuts at: 2687 2719 6224 6256 537
Size: 32 3505 32 988
Fragments arranged by size:
3505 988 889 444 399 290 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 304 3487 4504 304
Size: 3183 1017 2507
Fragments arranged by size:
3183 2507 1017
XbaI T'CTAG_A
Cuts at: 597 2682 3738 597
Size: 2085 1056 3566
Fragments arranged by size:
3566 2085 1056
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4202 4202
Size: 6707
XmaI C'CCGG_G
Cuts at: 3727 3727
Size: 6707
XmnI GAAnn'nnTTC
Cuts at: 3541 3541
Size: 6707
ZraI GAC'GTC
Cuts at: 3317 3317
Size: 6707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PpuMI
PshAI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AgeI AhdI ApaI AscI AvrII BaeI BcgI
BmgBI BmtI BsiWI BsmBI BssHII ClaI CspCI FseI
FspAI MfeI NdeI NheI NotI NruI PacI PasI
PfoI PmlI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI