Restriction map for
pF1KB6006
(Circular) (Six-base) MAPSORT of: pF1KB6006.seq Check: 8661 from: 1 to: 10517
pF1KB6006 10517 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 269 7347 269
Size: 7078 3439
AccI GT'mk_AC
Cuts at: 2867 3254 3472 3517 5097 7555 2867
Size: 387 218 45 1580 2458 5829
Fragments arranged by size:
5829 2458 1580 387 218 45
Acc65I G'GTAC_C
Cuts at: 1435 7403 7533 1435
Size: 5968 130 4419
Fragments arranged by size:
5968 4419 130
AclI AA'CG_TT
Cuts at: 10357 10357
Size: 10517
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 701 892 1493 1660 1762 1892 2066 2369
Size: 297 191 601 167 102 130 174 303
Cuts at: 2369 2663 2728 2825 2900 3176 3206 3251 3689
Size: 294 65 97 75 276 30 45 438
Cuts at: 3689 3698 3815 4217 4694 5076 5183 5927 5978
Size: 9 117 402 477 382 107 744 51
Cuts at: 5978 6188 6275 6476 6626 6668 8342 8774 9127
Size: 210 87 201 150 42 1674 432 353
Cuts at: 9127 9789 404
Size: 662 1132
Fragments arranged by size:
1674 1132 744 662 601 477 438 432
402 382 353 303 297 294 276 210
201 191 174 167 150 130 117 107
102 97 87 75 65 51 45 42
30 9
AflIII A'CryG_T
Cuts at: 2988 4730 7869 9669 9950 2988
Size: 1742 3139 1800 281 3555
Fragments arranged by size:
3555 3139 1800 1742 281
AhdI GACnn_n'nnGTC
Cuts at: 4269 5775 4269
Size: 1506 9011
AleI CACnn'nnGTG
Cuts at: 416 475 1690 1816 2993 416
Size: 59 1215 126 1177 7940
Fragments arranged by size:
7940 1215 1177 126 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 493 1423 1792 5096 7369 493
Size: 930 369 3304 2273 3641
Fragments arranged by size:
3641 3304 2273 930 369
AlwNI CAG_nnn'CTG
Cuts at: 1650 1915 2488 2527 2923 2950 3271 4444 4885
Size: 265 573 39 396 27 321 1173 441
Cuts at: 4885 6798 7822 9260 1650
Size: 1913 1024 1438 2907
Fragments arranged by size:
2907 1913 1438 1173 1024 573 441 396
321 265 39 27
ApaLI G'TGCA_C
Cuts at: 9355 9355
Size: 10517
ApoI r'AATT_y
Cuts at: 1456 2881 3442 3580 3757 4198 5050 7231 7521
Size: 1425 561 138 177 441 852 2181 290
Cuts at: 7521 9870 10126 1456
Size: 2349 256 1847
Fragments arranged by size:
2349 2181 1847 1425 852 561 441 290
256 177 138
AseI AT'TA_AT
Cuts at: 20 6413 20
Size: 6393 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 10517
AvaI C'yCGr_G
Cuts at: 1099 3524 7537 1099
Size: 2425 4013 4079
Fragments arranged by size:
4079 4013 2425
BamHI G'GATC_C
Cuts at: 4321 7542 4321
Size: 3221 7296
BanI G'GyrC_C
Cuts at: 1435 1680 3277 4378 7403 7533 7947 8195 8230
Size: 245 1597 1101 3025 130 414 248 35
Cuts at: 8230 1435
Size: 3722
Fragments arranged by size:
3722 3025 1597 1101 414 248 245 130
35
BanII G_rGCy'C
Cuts at: 290 557 1100 1123 2849 3330 3497 3877 4256
Size: 267 543 23 1726 481 167 380 379
Cuts at: 4256 4789 5267 5594 6495 7298 7531 8561 290
Size: 533 478 327 901 803 233 1030 2246
Fragments arranged by size:
2246 1726 1030 901 803 543 533 481
478 380 379 327 267 233 167 23
BbeI G_GCGC'C
Cuts at: 7951 8199 7951
Size: 248 10269
BbsI GAAGACnn'nnnn_
Cuts at: 2020 2054 2699 3119 3239 3434 3758 4523 4658
Size: 34 645 420 120 195 324 765 135
Cuts at: 4658 4733 5701 5794 5947 6656 6838 7244 2020
Size: 75 968 93 153 709 182 406 5293
Fragments arranged by size:
5293 968 765 709 645 420 406 324
195 182 153 135 120 93 75 34
BbvCI CC'TCA_GC
Cuts at: 2675 2960 4835 7289 2675
Size: 285 1875 2454 5903
Fragments arranged by size:
5903 2454 1875 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3244 3278 7079 7113 3244
Size: 34 3801 34 6648
Fragments arranged by size:
6648 3801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8415 9471 10517 8415
Size: 1056 1046 8415
Fragments arranged by size:
8415 1056 1046
BclI T'GATC_A
Cuts at: 443 488 8037 443
Size: 45 7549 2923
Fragments arranged by size:
7549 2923 45
BfrBI ATG'CAT
Cuts at: 3058 3058
Size: 10517
BglI GCCn_nnn'nGGC
Cuts at: 166 1966 2485 4777 166
Size: 1800 519 2292 5906
Fragments arranged by size:
5906 2292 1800 519
BglII A'GATC_T
Cuts at: 1485 3766 5023 6819 7431 8032 10069 1485
Size: 2281 1257 1796 612 601 2037 1933
Fragments arranged by size:
2281 2037 1933 1796 1257 612 601
BlpI GC'TnA_GC
Cuts at: 7629 7629
Size: 10517
Bme1580I G_kGCm'C
Cuts at: 1685 6616 7160 8142 8235 9359 1685
Size: 4931 544 982 93 1124 2843
Fragments arranged by size:
4931 2843 1124 982 544 93
BmgBI CAC'GTC
Cuts at: 3531 3531
Size: 10517
BmrI ACTGGGnnnn_n'
Cuts at: 1584 3324 7729 7810 8145 10289 1584
Size: 1740 4405 81 335 2144 1812
Fragments arranged by size:
4405 2144 1812 1740 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 822 3908 4860 5286 5455 5457 6633 8884 822
Size: 3086 952 426 169 2 1176 2251 2455
Fragments arranged by size:
3086 2455 2251 1176 952 426 169 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1246 1934 2531 2701 2863 2945 3401 4406
Size: 699 688 597 170 162 82 456 1005
Cuts at: 4406 5090 5181 5695 6488 6695 547
Size: 684 91 514 793 207 4369
Fragments arranged by size:
4369 1005 793 699 688 684 597 514
456 207 170 162 91 82
Bpu10I CC'TnA_GC
Cuts at: 2675 2960 4835 5069 7289 2675
Size: 285 1875 234 2220 5903
Fragments arranged by size:
5903 2220 1875 285 234
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1387 6448 7693 8572 9286 9584 1387
Size: 5061 1245 879 714 298 2320
Fragments arranged by size:
5061 2320 1245 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1891 3538 5872 7057 7405 10221 1891
Size: 1647 2334 1185 348 2816 2187
Fragments arranged by size:
2816 2334 2187 1647 1185 348
BsaAI yAC'GTr
Cuts at: 1827 2991 3778 4733 6689 7042 7263 7870 1827
Size: 1164 787 955 1956 353 221 607 4474
Fragments arranged by size:
4474 1956 1164 955 787 607 353 221
BsaBI GATnn'nnATC
Cuts at: 6 5252 8031 10068 6
Size: 5246 2779 2037 455
Fragments arranged by size:
5246 2779 2037 455
BsaHI Gr'CG_yC
Cuts at: 172 3952 7948 8196 8898 10394 172
Size: 3780 3996 248 702 1496 295
Fragments arranged by size:
3996 3780 1496 702 295 248
BsaWI w'CCGG_w
Cuts at: 547 7706 8227 8905 9316 9463 9737 547
Size: 7159 521 678 411 147 274 1327
Fragments arranged by size:
7159 1327 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 707 737 1141 1171 5218 5248 5835 5865 7005
Size: 30 404 30 4047 30 587 30 1140
Cuts at: 7005 7035 707
Size: 30 4189
Fragments arranged by size:
4189 4047 1140 587 404 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 2074 2330 2851 2968 2977 3175 3187 3214
Size: 1806 256 521 117 9 198 12 27
Cuts at: 3214 3298 3430 5242 5327 5360 7306 268
Size: 84 132 1812 85 33 1946 3479
Fragments arranged by size:
3479 1946 1812 1806 521 256 198 132
117 85 84 33 27 12 9
BseYI C'CCAG_C
Cuts at: 1930 6118 7297 7942 8700 9365 9773 1930
Size: 4188 1179 645 758 665 408 2674
Fragments arranged by size:
4188 2674 1179 758 665 645 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 5147 7022 5147
Size: 1875 8642
BsiEI CG_ry'CG
Cuts at: 104 116 6629 8059 8105 9335 104
Size: 12 6513 1430 46 1230 1286
Fragments arranged by size:
6513 1430 1286 1230 46 12
BsiHKAI G_wGCw'C
Cuts at: 260 290 557 1100 4789 5267 7531 8309 8499
Size: 30 267 543 3689 478 2264 778 190
Cuts at: 8499 9359 260
Size: 860 1418
Fragments arranged by size:
3689 2264 1418 860 778 543 478 267
190 30
BsmI GAATG_Cn'
Cuts at: 328 4948 7077 328
Size: 4620 2129 3768
Fragments arranged by size:
4620 3768 2129
BsmBI CGTCTCn'nnnn_
Cuts at: 3521 3553 3521
Size: 32 10485
Bsp1286I G_dGCh'C
Cuts at: 260 290 557 1100 1123 1685 2849 3330 3497
Size: 30 267 543 23 562 1164 481 167
Cuts at: 3497 3877 4256 4789 5267 5594 6495 6616 7160
Size: 380 379 533 478 327 901 121 544
Cuts at: 7160 7298 7531 8142 8235 8309 8499 8561 9359
Size: 138 233 611 93 74 190 62 798
Cuts at: 9359 260
Size: 1418
Fragments arranged by size:
1418 1164 901 798 611 562 544 543
533 481 478 380 379 327 267 233
190 167 138 121 93 74 62 30
23
BspHI T'CATG_A
Cuts at: 3363 7377 8949 3363
Size: 4014 1572 4931
Fragments arranged by size:
4931 4014 1572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 269 3357 3645 7347 7567 7781 8083 8464 269
Size: 3088 288 3702 220 214 302 381 2322
Fragments arranged by size:
3702 3088 2322 381 302 288 220 214
BsrBI CCG'CTC
Cuts at: 50 3654 8809 8863 10347 10512 50
Size: 3604 5155 54 1484 165 55
Fragments arranged by size:
5155 3604 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 6078 8429 6078
Size: 2351 8166
BsrFI r'CCGG_y
Cuts at: 2899 8515 8696 2899
Size: 5616 181 4720
Fragments arranged by size:
5616 4720 181
BssSI C'ACGA_G
Cuts at: 517 5474 6358 8788 9496 517
Size: 4957 884 2430 708 1538
Fragments arranged by size:
4957 2430 1538 884 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1546 7894 1546
Size: 6348 4169
BstBI TT'CG_AA
Cuts at: 8878 8878
Size: 10517
BstEII G'GTnAC_C
Cuts at: 4008 4008
Size: 10517
BstXI CCAn_nnnn'nTGG
Cuts at: 1692 3337 3504 6109 1692
Size: 1645 167 2605 6100
Fragments arranged by size:
6100 2605 1645 167
BstYI r'GATC_y
Cuts at: 1 401 1485 1665 2610 3766 4321 4639 5023
Size: 400 1084 180 945 1156 555 318 384
Cuts at: 5023 6819 7431 7542 8009 8032 8367 8613 8919
Size: 1796 612 111 467 23 335 246 306
Cuts at: 8919 8931 9017 10069 1
Size: 12 86 1052 449
Fragments arranged by size:
1796 1156 1084 1052 945 612 555 467
449 400 384 335 318 306 246 180
111 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1805 2606 2834 3437 4004 4046 5732 6263 7025
Size: 801 228 603 567 42 1686 531 762
Cuts at: 7025 1805
Size: 5297
Fragments arranged by size:
5297 1686 801 762 603 567 531 228
42
BtgI C'CryG_G
Cuts at: 107 116 3503 3743 4244 6761 107
Size: 9 3387 240 501 2517 3863
Fragments arranged by size:
3863 3387 2517 501 240 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 141 8467 8648 8811 141
Size: 8326 181 163 1847
Fragments arranged by size:
8326 1847 181 163
BtsI GCAGTG_nn'
Cuts at: 538 941 1432 3446 4101 6305 7112 7760 538
Size: 403 491 2014 655 2204 807 648 3295
Fragments arranged by size:
3295 2204 2014 807 655 648 491 403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 5147 5182 5147
Size: 35 10482
DraI TTT'AAA
Cuts at: 2372 4647 7516 2372
Size: 2275 2869 5373
Fragments arranged by size:
5373 2869 2275
DraIII CAC_nnn'GTG
Cuts at: 5704 5704
Size: 10517
DrdI GACnn_nn'nnGTC
Cuts at: 6042 6682 7143 8223 9567 6042
Size: 640 461 1080 1344 6992
Fragments arranged by size:
6992 1344 1080 640 461
EaeI y'GGCC_r
Cuts at: 1652 1674 3086 3339 4524 5055 6099 6626 6782
Size: 22 1412 253 1185 531 1044 527 156
Cuts at: 6782 7100 8102 8276 8667 8694 10039 1652
Size: 318 1002 174 391 27 1345 2130
Fragments arranged by size:
2130 1412 1345 1185 1044 1002 531 527
391 318 253 174 156 27 22
EagI C'GGCC_G
Cuts at: 6626 8102 6626
Size: 1476 9041
EarI CTCTTCn'nnn_
Cuts at: 1225 1934 2135 2174 2249 2390 2624 2639 2693
Size: 709 201 39 75 141 234 15 54
Cuts at: 2693 2930 2966 3067 3152 3551 3744 3865 5310
Size: 237 36 101 85 399 193 121 1445
Cuts at: 5310 5565 5954 6602 7043 8540 8750 1225
Size: 255 389 648 441 1497 210 2992
Fragments arranged by size:
2992 1497 1445 709 648 441 399 389
255 237 234 210 201 193 141 121
101 85 75 54 39 36 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 980 3061 8725 9467 9613 10327 980
Size: 2081 5664 742 146 714 1170
Fragments arranged by size:
5664 2081 1170 742 714 146
EcoICRI GAG'CTC
Cuts at: 288 555 1098 4787 5265 7529 288
Size: 267 543 3689 478 2264 3276
Fragments arranged by size:
3689 3276 2264 543 478 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 547 701 892 1246 1493 1660 1762 1892
Size: 143 154 191 354 247 167 102 130
Cuts at: 1892 1934 2066 2369 2531 2663 2701 2728 2825
Size: 42 132 303 162 132 38 27 97
Cuts at: 2825 2863 2900 2945 3176 3206 3251 3401 3689
Size: 38 37 45 231 30 45 150 288
Cuts at: 3689 3698 3815 4217 4406 4694 5076 5090 5181
Size: 9 117 402 189 288 382 14 91
Cuts at: 5181 5183 5695 5927 5978 6188 6275 6476 6488
Size: 2 512 232 51 210 87 201 12
Cuts at: 6488 6626 6668 6695 8342 8774 9127 9789 404
Size: 138 42 27 1647 432 353 662 1132
Fragments arranged by size:
1647 1132 662 512 432 402 382 354
353 303 288 288 247 232 231 210
201 191 189 167 162 154 150 143
138 132 132 130 117 102 97 91
87 51 45 45 42 42 38 38
37 30 27 27 14 12 9 2
EcoNI CCTnn'n_nnAGG
Cuts at: 683 1166 5677 6506 683
Size: 483 4511 829 4694
Fragments arranged by size:
4694 4511 829 483
EcoO109I rG'GnC_Cy
Cuts at: 2588 2767 5747 6114 7656 2588
Size: 179 2980 367 1542 5449
Fragments arranged by size:
5449 2980 1542 367 179
EcoRI G'AATT_C
Cuts at: 1456 3442 3580 4198 7521 1456
Size: 1986 138 618 3323 4452
Fragments arranged by size:
4452 3323 1986 618 138
EcoRV GAT'ATC
Cuts at: 3485 5457 3485
Size: 1972 8545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 237 8536 237
Size: 8299 2218
FspI TGC'GCA
Cuts at: 1780 8298 1780
Size: 6518 3999
HaeII r_GCGC'y
Cuts at: 3005 7951 8199 9429 10200 3005
Size: 4946 248 1230 771 3322
Fragments arranged by size:
4946 3322 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 386 393 418 425 707 739 964 996 3499
Size: 7 25 7 282 32 225 32 2503
Cuts at: 3499 3531 3550 3582 3820 3852 4123 4155 4255
Size: 32 19 32 238 32 271 32 100
Cuts at: 4255 4287 4720 4752 4801 4833 5008 5040 5218
Size: 32 433 32 49 32 175 32 178
Cuts at: 5218 5250 5319 5341 5351 5373 5503 5535 5566
Size: 32 69 22 10 22 130 32 31
Cuts at: 5566 5598 5761 5793 6883 6915 6943 6975 7359
Size: 32 163 32 1090 32 28 32 384
Cuts at: 7359 7391 8359 8389 8391 8421 386
Size: 32 968 30 2 30 2482
Fragments arranged by size:
2503 2482 1090 968 433 384 282 271
238 225 178 175 163 130 100 69
49 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 31 30 30 28 25 22
22 19 10 7 7 2
HincII GTy'rAC
Cuts at: 3255 5098 7556 3255
Size: 1843 2458 6216
Fragments arranged by size:
6216 2458 1843
KasI G'GCGC_C
Cuts at: 7947 8195 7947
Size: 248 10269
KpnI G_GTAC'C
Cuts at: 1439 7407 7537 1439
Size: 5968 130 4419
Fragments arranged by size:
5968 4419 130
MluI A'CGCG_T
Cuts at: 9950 9950
Size: 10517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1026 1592 6638 7304 9276 9460 1026
Size: 566 5046 666 1972 184 2083
Fragments arranged by size:
5046 2083 1972 666 566 184
MscI TGG'CCA
Cuts at: 1654 1676 3088 4526 5057 6101 6784 8278 1654
Size: 22 1412 1438 531 1044 683 1494 3893
Fragments arranged by size:
3893 1494 1438 1412 1044 683 531 22
MslI CAynn'nnrTG
Cuts at: 416 475 1225 1690 1816 2993 3502 4660 6752
Size: 59 750 465 126 1177 509 1158 2092
Cuts at: 6752 7357 8506 8633 416
Size: 605 1149 127 2300
Fragments arranged by size:
2300 2092 1177 1158 1149 750 605 509
465 127 126 59
MspA1I CmG'CkG
Cuts at: 1083 2365 2500 2527 2542 2679 3841 6931 7628
Size: 1282 135 27 15 137 1162 3090 697
Cuts at: 7628 7942 8302 9086 9331 1083
Size: 314 360 784 245 2269
Fragments arranged by size:
3090 2269 1282 1162 784 697 360 314
245 137 135 27 15
NaeI GCC'GGC
Cuts at: 8698 8698
Size: 10517
NarI GG'CG_CC
Cuts at: 7948 8196 7948
Size: 248 10269
NcoI C'CATG_G
Cuts at: 107 3503 3743 4244 6761 107
Size: 3396 240 501 2517 3863
Fragments arranged by size:
3863 3396 2517 501 240
NdeI CA'TA_TG
Cuts at: 4742 5976 4742
Size: 1234 9283
NgoMIV G'CCGG_C
Cuts at: 8696 8696
Size: 10517
NsiI A_TGCA'T
Cuts at: 3060 3060
Size: 10517
NspI r_CATG'y
Cuts at: 1077 4834 5190 7356 7499 7570 8070 9673 1077
Size: 3757 356 2166 143 71 500 1603 1921
Fragments arranged by size:
3757 2166 1921 1603 500 356 143 71
PasI CC'CwG_GG
Cuts at: 1116 3921 1116
Size: 2805 7712
PciI A'CATG_T
Cuts at: 9669 9669
Size: 10517
PflMI CCAn_nnn'nTGG
Cuts at: 1350 1350
Size: 10517
PfoI T'CCnGG_A
Cuts at: 903 1601 4910 4999 6504 7310 903
Size: 698 3309 89 1505 806 4110
Fragments arranged by size:
4110 3309 1505 806 698 89
PmeI GTTT'AAAC
Cuts at: 7516 7516
Size: 10517
PmlI CAC'GTG
Cuts at: 1827 7042 1827
Size: 5215 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8963 8995 8963
Size: 32 10485
PpuMI rG'GwC_Cy
Cuts at: 2588 2767 5747 6114 2588
Size: 179 2980 367 6991
Fragments arranged by size:
6991 2980 367 179
PshAI GACnn'nnGTC
Cuts at: 4956 5093 5174 4956
Size: 137 81 10299
Fragments arranged by size:
10299 137 81
PspXI vC'TCGA_Gb
Cuts at: 1099 1099
Size: 10517
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 10485
PstI C_TGCA'G
Cuts at: 2499 3267 3978 5526 6243 6312 7119 7564 8249
Size: 768 711 1548 717 69 807 445 685
Cuts at: 8249 2499
Size: 4767
Fragments arranged by size:
4767 1548 807 768 717 711 685 445
69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 10517
PvuII CAG'CTG
Cuts at: 2365 2500 2527 2542 2679 3841 7942 8302 2365
Size: 135 27 15 137 1162 4101 360 4580
Fragments arranged by size:
4580 4101 1162 360 137 135 27 15
RsrII CG'GwC_CG
Cuts at: 8712 8712
Size: 10517
SacI G_AGCT'C
Cuts at: 290 557 1100 4789 5267 7531 290
Size: 267 543 3689 478 2264 3276
Fragments arranged by size:
3689 3276 2264 543 478 267
SalI G'TCGA_C
Cuts at: 3253 5096 7554 3253
Size: 1843 2458 6216
Fragments arranged by size:
6216 2458 1843
SanDI GG'GwC_CC
Cuts at: 6114 6114
Size: 10517
SapI GCTCTTCn'nnn_
Cuts at: 1934 3865 8540 8750 1934
Size: 1931 4675 210 3701
Fragments arranged by size:
4675 3701 1931 210
SbfI CC_TGCA'GG
Cuts at: 7564 7564
Size: 10517
ScaI AGT'ACT
Cuts at: 10034 10034
Size: 10517
SexAI A'CCwGG_T
Cuts at: 5700 5743 5700
Size: 43 10474
SfcI C'TryA_G
Cuts at: 33 2495 3263 3974 4154 5117 5172 5522 6239
Size: 2462 768 711 180 963 55 350 717
Cuts at: 6239 6308 7115 7560 8245 9213 9404 33
Size: 69 807 445 685 968 191 1146
Fragments arranged by size:
2462 1146 968 963 807 768 717 711
685 445 350 191 180 69 55
SfoI GGC'GCC
Cuts at: 7949 8197 7949
Size: 248 10269
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 10517
SmaI CCC'GGG
Cuts at: 7539 7539
Size: 10517
SmlI C'TyrA_G
Cuts at: 1099 1366 3524 6427 7672 8587 9301 9563 1099
Size: 267 2158 2903 1245 915 714 262 2053
Fragments arranged by size:
2903 2158 2053 1245 915 714 267 262
SpeI A'CTAG_T
Cuts at: 67 4459 67
Size: 4392 6125
SphI G_CATG'C
Cuts at: 4834 7499 7570 8070 4834
Size: 2665 71 500 7281
Fragments arranged by size:
7281 2665 500 71
SspI AAT'ATT
Cuts at: 741 741
Size: 10517
StuI AGG'CCT
Cuts at: 5290 6778 7166 5290
Size: 1488 388 8641
Fragments arranged by size:
8641 1488 388
StyI C'CwwG_G
Cuts at: 107 2006 2618 3041 3503 3743 4244 6761 6779
Size: 1899 612 423 462 240 501 2517 18
Cuts at: 6779 6908 7651 8005 107
Size: 129 743 354 2619
Fragments arranged by size:
2619 2517 1899 743 612 501 462 423
354 240 129 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 265 655 1028 1051 1603 1672 1854 3116 3913
Size: 390 373 23 552 69 182 1262 797
Cuts at: 3913 4968 5113 5257 5800 6595 8100 8233 8692
Size: 1055 145 144 543 795 1505 133 459
Cuts at: 8692 8901 265
Size: 209 1881
Fragments arranged by size:
1881 1505 1262 1055 797 795 552 543
459 390 373 209 182 145 144 133
69 23
TatI w'GTAC_w
Cuts at: 1592 10032 1592
Size: 8440 2077
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 627 659 2751 2783 5835 5867 7005 7037 10034
Size: 32 2092 32 3052 32 1138 32 2997
Cuts at: 10034 10066 627
Size: 32 1078
Fragments arranged by size:
3052 2997 2092 1138 1078 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3564 6864 7143 8314 3564
Size: 3300 279 1171 5767
Fragments arranged by size:
5767 3300 1171 279
XbaI T'CTAG_A
Cuts at: 1629 6046 7548 1629
Size: 4417 1502 4598
Fragments arranged by size:
4598 4417 1502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 3813 4773 5670 8012 114
Size: 3699 960 897 2342 2619
Fragments arranged by size:
3699 2619 2342 960 897
XhoI C'TCGA_G
Cuts at: 1099 3524 1099
Size: 2425 8092
XmaI C'CCGG_G
Cuts at: 7537 7537
Size: 10517
XmnI GAAnn'nnTTC
Cuts at: 943 1460 4343 943
Size: 517 2883 7117
Fragments arranged by size:
7117 2883 517
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AloI ApaI AscI AvrII
BaeI BmtI BsiWI BspEI BsrGI BssHII BstZ17I ClaI
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
PacI PsiI PspOMI SacII SfiI SgrAI SnaBI SrfI
SwaI ZraI