Restriction map for
pF1KB6007
(Circular) (Six-base) MAPSORT of: pF1KB6007.seq Check: 1935 from: 1 to: 5615
pF1KB6007 5615 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 1526 1633 1526
Size: 107 5508
AccI GT'mk_AC
Cuts at: 971 2162 2653 971
Size: 1191 491 3933
Fragments arranged by size:
3933 1191 491
Acc65I G'GTAC_C
Cuts at: 2631 2631
Size: 5615
AclI AA'CG_TT
Cuts at: 5455 5455
Size: 5615
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 618 835 1315 3440 3872 4225 4887 618
Size: 217 480 2125 432 353 662 1346
Fragments arranged by size:
2125 1346 662 480 432 353 217
AfeI AGC'GCT
Cuts at: 1504 1882 1504
Size: 378 5237
AflIII A'CryG_T
Cuts at: 986 2039 2967 4767 5048 986
Size: 1053 928 1800 281 1553
Fragments arranged by size:
1800 1553 1053 928 281
AleI CACnn'nnGTG
Cuts at: 1537 2128 1537
Size: 591 5024
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1168 1772 1168
Size: 604 5011
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 424 456 833 865 424
Size: 32 377 32 5174
Fragments arranged by size:
5174 377 32 32
AlwNI CAG_nnn'CTG
Cuts at: 526 1063 2920 4358 526
Size: 537 1857 1438 1783
Fragments arranged by size:
1857 1783 1438 537
ApaI G_GGCC'C
Cuts at: 1869 1869
Size: 5615
ApaLI G'TGCA_C
Cuts at: 487 4453 487
Size: 3966 1649
ApoI r'AATT_y
Cuts at: 311 829 1735 1896 1933 2619 4968 5224 311
Size: 518 906 161 37 686 2349 256 702
Fragments arranged by size:
2349 906 702 686 518 256 161 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 5615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
AvaI C'yCGr_G
Cuts at: 189 908 1797 2635 189
Size: 719 889 838 3169
Fragments arranged by size:
3169 889 838 719
BamHI G'GATC_C
Cuts at: 848 2640 848
Size: 1792 3823
BanI G'GyrC_C
Cuts at: 1152 2208 2631 3045 3293 3328 1152
Size: 1056 423 414 248 35 3439
Fragments arranged by size:
3439 1056 423 414 248 35
BanII G_rGCy'C
Cuts at: 131 952 1052 1869 2629 3659 131
Size: 821 100 817 760 1030 2087
Fragments arranged by size:
2087 1030 821 817 760 100
BbeI G_GCGC'C
Cuts at: 2212 3049 3297 2212
Size: 837 248 4530
Fragments arranged by size:
4530 837 248
BbsI GAAGACnn'nnnn_
Cuts at: 129 302 658 676 770 986 1410 2524 129
Size: 173 356 18 94 216 424 1114 3220
Fragments arranged by size:
3220 1114 424 356 216 173 94 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1965 1999 1965
Size: 34 5581
BciVI GTATCCnnnnn_n'
Cuts at: 3513 4569 5615 3513
Size: 1056 1046 3513
Fragments arranged by size:
3513 1056 1046
BclI T'GATC_A
Cuts at: 1856 3135 1856
Size: 1279 4336
BfrBI ATG'CAT
Cuts at: 1275 1275
Size: 5615
BglII A'GATC_T
Cuts at: 3130 5167 3130
Size: 2037 3578
BlpI GC'TnA_GC
Cuts at: 505 2727 505
Size: 2222 3393
Bme1580I G_kGCm'C
Cuts at: 491 1869 3240 3333 4457 491
Size: 1378 1371 93 1124 1649
Fragments arranged by size:
1649 1378 1371 1124 93
BmgBI CAC'GTC
Cuts at: 768 768
Size: 5615
BmrI ACTGGGnnnn_n'
Cuts at: 536 678 2487 2827 2908 3243 5387 536
Size: 142 1809 340 81 335 2144 764
Fragments arranged by size:
2144 1809 764 340 335 142 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 3982 811
Size: 3171 2444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 886 2032 2359 514
Size: 372 1146 327 3770
Fragments arranged by size:
3770 1146 372 327
Bpu10I CC'TnA_GC
Cuts at: 2182 2182
Size: 5615
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2791 3670 4384 4682 2791
Size: 879 714 298 3724
Fragments arranged by size:
3724 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1246 1802 2425 2584 5319 1246
Size: 556 623 159 2735 1542
Fragments arranged by size:
2735 1542 623 556 159
BsaAI yAC'GTr
Cuts at: 456 987 2968 456
Size: 531 1981 3103
Fragments arranged by size:
3103 1981 531
BsaBI GATnn'nnATC
Cuts at: 6 157 3129 5166 6
Size: 151 2972 2037 455
Fragments arranged by size:
2972 2037 455 151
BsaHI Gr'CG_yC
Cuts at: 1234 1523 1630 2209 3046 3294 3996 5492 1234
Size: 289 107 579 837 248 702 1496 1357
Fragments arranged by size:
1496 1357 837 702 579 289 248 107
BsaWI w'CCGG_w
Cuts at: 365 2804 3325 4003 4414 4561 4835 365
Size: 2439 521 678 411 147 274 1145
Fragments arranged by size:
2439 1145 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 312 342 934 964 1039 1069 2005 2035 312
Size: 30 592 30 75 30 936 30 3892
Fragments arranged by size:
3892 936 592 75 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 772 1324 2029 2032 2410 256
Size: 516 552 705 3 378 3461
Fragments arranged by size:
3461 705 552 516 378 3
BseYI C'CCAG_C
Cuts at: 2256 2461 3040 3798 4463 4871 2256
Size: 205 579 758 665 408 3000
Fragments arranged by size:
3000 758 665 579 408 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 204 496 2226 204
Size: 292 1730 3593
Fragments arranged by size:
3593 1730 292
BsiEI CG_ry'CG
Cuts at: 104 1112 1457 3157 3203 4433 104
Size: 1008 345 1700 46 1230 1286
Fragments arranged by size:
1700 1286 1230 1008 345 46
BsiHKAI G_wGCw'C
Cuts at: 491 2489 2543 2629 3407 3597 4457 491
Size: 1998 54 86 778 190 860 1649
Fragments arranged by size:
1998 1649 860 778 190 86 54
BsmI GAATG_Cn'
Cuts at: 807 1976 807
Size: 1169 4446
BsmBI CGTCTCn'nnnn_
Cuts at: 350 2287 350
Size: 1937 3678
Bsp1286I G_dGCh'C
Cuts at: 131 491 952 1052 1869 2489 2543 2629 3240
Size: 360 461 100 817 620 54 86 611
Cuts at: 3240 3333 3407 3597 3659 4457 131
Size: 93 74 190 62 798 1289
Fragments arranged by size:
1289 817 798 620 611 461 360 190
100 93 86 74 62 54
BspEI T'CCGG_A
Cuts at: 365 365
Size: 5615
BspHI T'CATG_A
Cuts at: 1853 4047 1853
Size: 2194 3421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1031 1289 2665 2879 3181 3562 1031
Size: 258 1376 214 302 381 3084
Fragments arranged by size:
3084 1376 381 302 258 214
BsrBI CCG'CTC
Cuts at: 50 2186 3907 3961 5445 5610 50
Size: 2136 1721 54 1484 165 55
Fragments arranged by size:
2136 1721 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 601 3527 601
Size: 2926 2689
BsrFI r'CCGG_y
Cuts at: 3613 3794 3613
Size: 181 5434
BsrGI T'GTAC_A
Cuts at: 2072 2324 2072
Size: 252 5363
BssSI C'ACGA_G
Cuts at: 3886 4594 3886
Size: 708 4907
BstAPI GCAn_nnn'nTGC
Cuts at: 1281 2992 1281
Size: 1711 3904
BstBI TT'CG_AA
Cuts at: 3976 3976
Size: 5615
BstEII G'GTnAC_C
Cuts at: 711 711
Size: 5615
BstXI CCAn_nnnn'nTGG
Cuts at: 114 264 1263 114
Size: 150 999 4466
Fragments arranged by size:
4466 999 150
BstYI r'GATC_y
Cuts at: 1 848 1782 2640 3107 3130 3465 3711 4017
Size: 847 934 858 467 23 335 246 306
Cuts at: 4017 4029 4115 5167 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 934 858 847 467 449 335 306
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 972 972
Size: 5615
Bsu36I CC'TnA_GG
Cuts at: 1558 1558
Size: 5615
BtgI C'CryG_G
Cuts at: 107 107
Size: 5615
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1155 2442 3565 3746 3909 1155
Size: 1287 1123 181 163 2861
Fragments arranged by size:
2861 1287 1123 181 163
BtsI GCAGTG_nn'
Cuts at: 1532 1544 2006 2858 1532
Size: 12 462 852 4289
Fragments arranged by size:
4289 852 462 12
DraI TTT'AAA
Cuts at: 2614 2614
Size: 5615
DrdI GACnn_nn'nnGTC
Cuts at: 529 1522 3321 4665 529
Size: 993 1799 1344 1479
Fragments arranged by size:
1799 1479 1344 993
EaeI y'GGCC_r
Cuts at: 392 1109 1496 2187 3200 3374 3765 3792 5137
Size: 717 387 691 1013 174 391 27 1345
Cuts at: 5137 392
Size: 870
Fragments arranged by size:
1345 1013 870 717 691 391 387 174
27
EagI C'GGCC_G
Cuts at: 1109 3200 1109
Size: 2091 3524
EarI CTCTTCn'nnn_
Cuts at: 658 1135 1600 1800 1900 2320 3638 3848 658
Size: 477 465 200 100 420 1318 210 2425
Fragments arranged by size:
2425 1318 477 465 420 210 200 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2163 3823 4565 4711 5425 2163
Size: 1660 742 146 714 2353
Fragments arranged by size:
2353 1660 742 714 146
EcoICRI GAG'CTC
Cuts at: 2627 2627
Size: 5615
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 618 835 886 1315 2032 2359 3440 3872
Size: 104 217 51 429 717 327 1081 432
Cuts at: 3872 4225 4887 514
Size: 353 662 1242
Fragments arranged by size:
1242 1081 717 662 432 429 353 327
217 104 51
EcoNI CCTnn'n_nnAGG
Cuts at: 235 1081 1486 235
Size: 846 405 4364
Fragments arranged by size:
4364 846 405
EcoO109I rG'GnC_Cy
Cuts at: 573 628 1020 1200 1865 2754 573
Size: 55 392 180 665 889 3434
Fragments arranged by size:
3434 889 665 392 180 55
EcoRI G'AATT_C
Cuts at: 829 1735 2619 829
Size: 906 884 3825
Fragments arranged by size:
3825 906 884
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 426 762 1794 3634 426
Size: 336 1032 1840 2407
Fragments arranged by size:
2407 1840 1032 336
FspI TGC'GCA
Cuts at: 3396 3396
Size: 5615
HaeII r_GCGC'y
Cuts at: 1506 1884 2154 2212 3049 3297 4527 5298 1506
Size: 378 270 58 837 248 1230 771 1823
Fragments arranged by size:
1823 1230 837 771 378 270 248 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 312 344 451 483 663 695 934 966 1039
Size: 32 107 32 180 32 239 32 73
Cuts at: 1039 1071 2005 2037 2583 2615 3457 3487 3489
Size: 32 934 32 546 32 842 30 2
Cuts at: 3489 3519 312
Size: 30 2408
Fragments arranged by size:
2408 934 842 546 239 180 107 73
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 792 2654 792
Size: 1862 3753
KasI G'GCGC_C
Cuts at: 2208 3045 3293 2208
Size: 837 248 4530
Fragments arranged by size:
4530 837 248
KpnI G_GTAC'C
Cuts at: 2635 2635
Size: 5615
MluI A'CGCG_T
Cuts at: 5048 5048
Size: 5615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4374 4558 2240
Size: 2134 184 3297
Fragments arranged by size:
3297 2134 184
MscI TGG'CCA
Cuts at: 394 3376 394
Size: 2982 2633
MslI CAynn'nnrTG
Cuts at: 112 1537 2128 3604 3731 112
Size: 1425 591 1476 127 1996
Fragments arranged by size:
1996 1476 1425 591 127
MspA1I CmG'CkG
Cuts at: 447 1119 2271 2726 3040 3400 4184 4429 447
Size: 672 1152 455 314 360 784 245 1633
Fragments arranged by size:
1633 1152 784 672 455 360 314 245
NaeI GCC'GGC
Cuts at: 3796 3796
Size: 5615
NarI GG'CG_CC
Cuts at: 2209 3046 3294 2209
Size: 837 248 4530
Fragments arranged by size:
4530 837 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5615
NgoMIV G'CCGG_C
Cuts at: 3794 3794
Size: 5615
NsiI A_TGCA'T
Cuts at: 1277 1277
Size: 5615
NspI r_CATG'y
Cuts at: 1288 2043 2668 3168 4771 1288
Size: 755 625 500 1603 2132
Fragments arranged by size:
2132 1603 755 625 500
PciI A'CATG_T
Cuts at: 2039 4767 2039
Size: 2728 2887
PflMI CCAn_nnn'nTGG
Cuts at: 1077 2108 1077
Size: 1031 4584
PfoI T'CCnGG_A
Cuts at: 1484 1508 1633 1484
Size: 24 125 5466
Fragments arranged by size:
5466 125 24
PmeI GTTT'AAAC
Cuts at: 2614 2614
Size: 5615
PmlI CAC'GTG
Cuts at: 456 987 456
Size: 531 5084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 4061
Size: 32 5583
PpuMI rG'GwC_Cy
Cuts at: 628 1020 1200 628
Size: 392 180 5043
Fragments arranged by size:
5043 392 180
PshAI GACnn'nnGTC
Cuts at: 2410 2410
Size: 5615
PspOMI G'GGCC_C
Cuts at: 1865 1865
Size: 5615
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 955 987 2392 2424 955
Size: 32 1405 32 4146
Fragments arranged by size:
4146 1405 32 32
PstI C_TGCA'G
Cuts at: 452 1539 2662 3347 452
Size: 1087 1123 685 2720
Fragments arranged by size:
2720 1123 1087 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5615
PvuII CAG'CTG
Cuts at: 447 2271 3040 3400 447
Size: 1824 769 360 2662
Fragments arranged by size:
2662 1824 769 360
RsrII CG'GwC_CG
Cuts at: 3810 3810
Size: 5615
SacI G_AGCT'C
Cuts at: 2629 2629
Size: 5615
SalI G'TCGA_C
Cuts at: 2652 2652
Size: 5615
SapI GCTCTTCn'nnn_
Cuts at: 1135 3638 3848 1135
Size: 2503 210 2902
Fragments arranged by size:
2902 2503 210
SbfI CC_TGCA'GG
Cuts at: 2662 2662
Size: 5615
ScaI AGT'ACT
Cuts at: 5132 5132
Size: 5615
SexAI A'CCwGG_T
Cuts at: 436 436
Size: 5615
SfcI C'TryA_G
Cuts at: 33 448 723 729 1535 1971 2202 2658 3343
Size: 415 275 6 806 436 231 456 685
Cuts at: 3343 4311 4502 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 806 685 456 436 415 275
231 191 6
SfoI GGC'GCC
Cuts at: 2210 3047 3295 2210
Size: 837 248 4530
Fragments arranged by size:
4530 837 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5615
SmaI CCC'GGG
Cuts at: 191 910 2637 191
Size: 719 1727 3169
Fragments arranged by size:
3169 1727 719
SmlI C'TyrA_G
Cuts at: 2770 3685 4399 4661 2770
Size: 915 714 262 3724
Fragments arranged by size:
3724 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5615
SphI G_CATG'C
Cuts at: 2668 3168 2668
Size: 500 5115
StuI AGG'CCT
Cuts at: 2356 2356
Size: 5615
StyI C'CwwG_G
Cuts at: 107 1869 2102 2749 3103 107
Size: 1762 233 647 354 2619
Fragments arranged by size:
2619 1762 647 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3198 3331 3790 3999 3198
Size: 133 459 209 4814
Fragments arranged by size:
4814 459 209 133
TatI w'GTAC_w
Cuts at: 2072 2324 5130 2072
Size: 252 2806 2557
Fragments arranged by size:
2806 2557 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5132 5164 5132
Size: 32 5583
Tth111I GACn'n_nGTC
Cuts at: 1414 3412 1414
Size: 1998 3617
XbaI T'CTAG_A
Cuts at: 2646 2646
Size: 5615
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 618 2091 2268 3110 618
Size: 1473 177 842 3123
Fragments arranged by size:
3123 1473 842 177
XmaI C'CCGG_G
Cuts at: 189 908 2635 189
Size: 719 1727 3169
Fragments arranged by size:
3169 1727 719
XmnI GAAnn'nnTTC
Cuts at: 1774 1774
Size: 5615
ZraI GAC'GTC
Cuts at: 1524 1631 1524
Size: 107 5508
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AhdI AscI AvrII BaeI BbvCI
BglI BmtI BsiWI BssHII ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PsiI PspXI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI