Restriction map for pF1KB6014
 (Circular) (Six-base) MAPSORT of: pF1KB6014.seq  Check: 2141  from: 1  to: 5207



pF1KB6014  5207 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:   2245    2245 
   Size:       5207
 
Acc65I G'GTAC_C

Cuts at:   2223    2223 
   Size:       5207
 
AclI AA'CG_TT

Cuts at:   5047    5047 
   Size:       5207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    857    1177    1675    3032    3464    3817    4479     857 
   Size:        320     498    1357     432     353     662    1585

  Fragments arranged by size: 

                1585    1357     662     498     432     353     320
 
AflIII A'CryG_T

Cuts at:   1814    2559    4359    4640    1814 
   Size:        745    1800     281    2381

  Fragments arranged by size: 

                2381    1800     745     281
 
AhdI GACnn_n'nnGTC

Cuts at:   1089    1089 
   Size:       5207
 
AlwNI CAG_nnn'CTG

Cuts at:   2013    2512    3950    2013 
   Size:        499    1438    3270

  Fragments arranged by size: 

                3270    1438     499
 
ApaI G_GGCC'C

Cuts at:    417    1957    2024    2031     417 
   Size:       1540      67       7    3593

  Fragments arranged by size: 

                3593    1540      67       7
 
ApaLI G'TGCA_C

Cuts at:   1946    4045    1946 
   Size:       2099    3108
 
ApoI r'AATT_y

Cuts at:    576    1029    1054    2211    4560    4816     576 
   Size:        453      25    1157    2349     256     967

  Fragments arranged by size: 

                2349    1157     967     453     256      25
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5207
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5207
 
AvaI C'yCGr_G

Cuts at:    141     824    2023    2062    2227     141 
   Size:        683    1199      39     165    3121

  Fragments arranged by size: 

                3121    1199     683     165      39
 
BamHI G'GATC_C

Cuts at:   1271    1671    2232    1271 
   Size:        400     561    4246

  Fragments arranged by size: 

                4246     561     400
 
BanI G'GyrC_C

Cuts at:   2223    2637    2885    2920    2223 
   Size:        414     248      35    4510

  Fragments arranged by size: 

                4510     414     248      35
 
BanII G_rGCy'C

Cuts at:    174     417    1957    2024    2031    2221    3251     174 
   Size:        243    1540      67       7     190    1030    2130

  Fragments arranged by size: 

                2130    1540    1030     243     190      67       7
 
BbeI G_GCGC'C

Cuts at:   2641    2889    2641 
   Size:        248    4959
 
BbsI GAAGACnn'nnnn_

Cuts at:   1165    1481    1165 
   Size:        316    4891
 
BciVI GTATCCnnnnn_n'

Cuts at:   3105    4161    5207    3105 
   Size:       1056    1046    3105

  Fragments arranged by size: 

                3105    1056    1046
 
BclI T'GATC_A

Cuts at:   2727    2727 
   Size:       5207
 
BglI GCCn_nnn'nGGC

Cuts at:    112     124     408    1655     112 
   Size:         12     284    1247    3664

  Fragments arranged by size: 

                3664    1247     284      12
 
BglII A'GATC_T

Cuts at:   2722    4759    2722 
   Size:       2037    3170
 
BlpI GC'TnA_GC

Cuts at:   2319    2319 
   Size:       5207
 
Bme1580I G_kGCm'C

Cuts at:    417    1950    1957    2024    2031    2832    2925    4049     417 
   Size:       1533       7      67       7     801      93    1124    1575

  Fragments arranged by size: 

                1575    1533    1124     801      93      67       7       7
            
 
BmrI ACTGGGnnnn_n'

Cuts at:    447     689    1960    2419    2500    2835    4979     447 
   Size:        242    1271     459      81     335    2144     675

  Fragments arranged by size: 

                2144    1271     675     459     335     242      81
 
BmtI G_CTAG'C

Cuts at:    229     229 
   Size:       5207
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3574    3574 
   Size:       5207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    590     874    1108    1990    2061     590 
   Size:        284     234     882      71    3736

  Fragments arranged by size: 

                3736     882     284     234      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1150    2383    3262    3976    4274    1150 
   Size:       1233     879     714     298    2083

  Fragments arranged by size: 

                2083    1233     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1907    4911    1907 
   Size:       3004    2203
 
BsaAI yAC'GTr

Cuts at:    738    1767    1817    2112    2560     738 
   Size:       1029      50     295     448    3385

  Fragments arranged by size: 

                3385    1029     448     295      50
 
BsaBI GATnn'nnATC

Cuts at:      6    2721    4758       6 
   Size:       2715    2037     455

  Fragments arranged by size: 

                2715    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    290    2638    2886    3588    5084     290 
   Size:       2348     248     702    1496     413

  Fragments arranged by size: 

                2348    1496     702     413     248
 
BsaWI w'CCGG_w

Cuts at:    533    1704    2396    2917    3595    4006    4153    4427     533 
   Size:       1171     692     521     678     411     147     274    1313

  Fragments arranged by size: 

                1313    1171     692     678     521     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1091    1121    1091 
   Size:         30    5177
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1521    1891    1521 
   Size:        370    4837
 
BseYI C'CCAG_C

Cuts at:    421    1716    1751    2632    3390    4055    4463     421 
   Size:       1295      35     881     758     665     408    1165

  Fragments arranged by size: 

                1295    1165     881     758     665     408      35
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    379     379 
   Size:       5207
 
BsiEI CG_ry'CG

Cuts at:    104     119     347    1210    1647    2198    2749    2795    4025 
   Size:         15     228     863     437     551     551      46    1230

Cuts at:   4025     104 
   Size:       1286

  Fragments arranged by size: 

                1286    1230     863     551     551     437     228      46
                  15
 
BsiHKAI G_wGCw'C

Cuts at:    831    1553    1772    1950    2221    2999    3189    4049     831 
   Size:        722     219     178     271     778     190     860    1989

  Fragments arranged by size: 

                1989     860     778     722     271     219     190     178
            
 
Bsp1286I G_dGCh'C

Cuts at:    174     417     831    1553    1772    1950    1957    2024    2031 
   Size:        243     414     722     219     178       7      67       7

Cuts at:   2031    2221    2832    2925    2999    3189    3251    4049     174 
   Size:        190     611      93      74     190      62     798    1332

  Fragments arranged by size: 

                1332     798     722     611     414     243     219     190
                 190     178      93      74      67      62       7       7
            
 
BspEI T'CCGG_A

Cuts at:   1704    1704 
   Size:       5207
 
BspHI T'CATG_A

Cuts at:   3639    3639 
   Size:       5207
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    168    2257    2471    2773    3154     168 
   Size:       2089     214     302     381    2221

  Fragments arranged by size: 

                2221    2089     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     154    3499    3553    5037    5202      50 
   Size:        104    3345      54    1484     165      55

  Fragments arranged by size: 

                3345    1484     165     104      55      54
 
BsrDI GCAATG_nn'

Cuts at:    970    3119     970 
   Size:       2149    3058
 
BsrFI r'CCGG_y

Cuts at:    656    3205    3386     656 
   Size:       2549     181    2477

  Fragments arranged by size: 

                2549    2477     181
 
BsrGI T'GTAC_A

Cuts at:   1073    1073 
   Size:       5207
 
BssHII G'CGCG_C

Cuts at:    133     133 
   Size:       5207
 
BssSI C'ACGA_G

Cuts at:    355    3478    4186     355 
   Size:       3123     708    1376

  Fragments arranged by size: 

                3123    1376     708
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2584    2584 
   Size:       5207
 
BstBI TT'CG_AA

Cuts at:   3568    3568 
   Size:       5207
 
BstEII G'GTnAC_C

Cuts at:    819    1387     819 
   Size:        568    4639
 
BstYI r'GATC_y

Cuts at:      1    1271    1671    2232    2699    2722    3057    3303    3609 
   Size:       1270     400     561     467      23     335     246     306

Cuts at:   3609    3621    3707    4759       1 
   Size:         12      86    1052     449

  Fragments arranged by size: 

                1270    1052     561     467     449     400     335     306
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     190     208     723    1577    1908    2198     107 
   Size:         83      18     515     854     331     290    3116

  Fragments arranged by size: 

                3116     854     515     331     290      83      18
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3157    3338    3501    3157 
   Size:        181     163    4863

  Fragments arranged by size: 

                4863     181     163
 
BtsI GCAGTG_nn'

Cuts at:    384    2450     384 
   Size:       2066    3141
 
DraI TTT'AAA

Cuts at:    543     582    2206     543 
   Size:         39    1624    3544

  Fragments arranged by size: 

                3544    1624      39
 
DraIII CAC_nnn'GTG

Cuts at:   1945    1945 
   Size:       5207
 
DrdI GACnn_nn'nnGTC

Cuts at:   2913    4257    2913 
   Size:       1344    3863
 
EaeI y'GGCC_r

Cuts at:    116     344     381    1142    1187    1207    1250    1644    1745 
   Size:        228      37     761      45      20      43     394     101

Cuts at:   1745    1794    1859    2195    2792    2966    3357    3384    4729 
   Size:         49      65     336     597     174     391      27    1345

Cuts at:   4729     116 
   Size:        594

  Fragments arranged by size: 

                1345     761     597     594     394     391     336     228
                 174     101      65      49      45      43      37      27
                  20
 
EagI C'GGCC_G

Cuts at:    116     344    1207    1644    2195    2792     116 
   Size:        228     863     437     551     597    2531

  Fragments arranged by size: 

                2531     863     597     551     437     228
 
EarI CTCTTCn'nnn_

Cuts at:    473    1868    3230    3440     473 
   Size:       1395    1362     210    2240

  Fragments arranged by size: 

                2240    1395    1362     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3415    4157    4303    5017    3415 
   Size:        742     146     714    3605

  Fragments arranged by size: 

                3605     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   2219    2219 
   Size:       5207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    590     857     874    1108    1177    1675    1990    2061    3032 
   Size:        267      17     234      69     498     315      71     971

Cuts at:   3032    3464    3817    4479     590 
   Size:        432     353     662    1318

  Fragments arranged by size: 

                1318     971     662     498     432     353     315     267
                 234      71      69      17
 
EcoO109I rG'GnC_Cy

Cuts at:    157     414     586    1740    1954    2027    2346     157 
   Size:        257     172    1154     214      73     319    3018

  Fragments arranged by size: 

                3018    1154     319     257     214     172      73
 
EcoRI G'AATT_C

Cuts at:   2211    2211 
   Size:       5207
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3226    3226 
   Size:       5207
 
FspI TGC'GCA

Cuts at:   2988    2988 
   Size:       5207
 
HaeII r_GCGC'y

Cuts at:    180    2641    2889    4119    4890     180 
   Size:       2461     248    1230     771     497

  Fragments arranged by size: 

                2461    1230     771     497     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1075    1107    1656    1688    1920    1952    1968    2000    3049 
   Size:         32     549      32     232      32      16      32    1049

Cuts at:   3049    3079    3081    3111    1075 
   Size:         30       2      30    3171

  Fragments arranged by size: 

                3171    1049     549     232      32      32      32      32
                  30      30      16       2
 
HincII GTy'rAC

Cuts at:   1302    2070    2246    1302 
   Size:        768     176    4263

  Fragments arranged by size: 

                4263     768     176
 
HindIII A'AGCT_T

Cuts at:   1687    1687 
   Size:       5207
 
KasI G'GCGC_C

Cuts at:   2637    2885    2637 
   Size:        248    4959
 
KpnI G_GTAC'C

Cuts at:   2227    2227 
   Size:       5207
 
MluI A'CGCG_T

Cuts at:   4640    4640 
   Size:       5207
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1208    1335    3966    4150    1208 
   Size:        127    2631     184    2265

  Fragments arranged by size: 

                2631    2265     184     127
 
MscI TGG'CCA

Cuts at:    383    1189    1252    1747    1861    2968     383 
   Size:        806      63     495     114    1107    2622

  Fragments arranged by size: 

                2622    1107     806     495     114      63
 
MslI CAynn'nnrTG

Cuts at:    817    1448    1664    2137    3196    3323     817 
   Size:        631     216     473    1059     127    2701

  Fragments arranged by size: 

                2701    1059     631     473     216     127
 
MspA1I CmG'CkG

Cuts at:    192     210     311    1751    2318    2632    2992    3776    4021 
   Size:         18     101    1440     567     314     360     784     245

Cuts at:   4021     192 
   Size:       1378

  Fragments arranged by size: 

                1440    1378     784     567     360     314     245     101
                  18
 
NaeI GCC'GGC

Cuts at:    658    3388     658 
   Size:       2730    2477
 
NarI GG'CG_CC

Cuts at:   2638    2886    2638 
   Size:        248    4959
 
NcoI C'CATG_G

Cuts at:    107    1577    1908     107 
   Size:       1470     331    3406

  Fragments arranged by size: 

                3406    1470     331
 
NgoMIV G'CCGG_C

Cuts at:    656    3386     656 
   Size:       2730    2477
 
NheI G'CTAG_C

Cuts at:    225     225 
   Size:       5207
 
NspI r_CATG'y

Cuts at:   2260    2760    4363    2260 
   Size:        500    1603    3104

  Fragments arranged by size: 

                3104    1603     500
 
PciI A'CATG_T

Cuts at:   4359    4359 
   Size:       5207
 
PflMI CCAn_nnn'nTGG

Cuts at:    738    1613    2013    2171     738 
   Size:        875     400     158    3774

  Fragments arranged by size: 

                3774     875     400     158
 
PmeI GTTT'AAAC

Cuts at:   2206    2206 
   Size:       5207
 
PmlI CAC'GTG

Cuts at:   1767    1767 
   Size:       5207
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3653    3685    3653 
   Size:         32    5175
 
PpuMI rG'GwC_Cy

Cuts at:    157     586     157 
   Size:        429    4778
 
PshAI GACnn'nnGTC

Cuts at:   2090    2090 
   Size:       5207
 
PspOMI G'GGCC_C

Cuts at:    413    1953    2020    2027     413 
   Size:       1540      67       7    3593

  Fragments arranged by size: 

                3593    1540      67       7
 
PstI C_TGCA'G

Cuts at:    638    1241    1499    2254    2939     638 
   Size:        603     258     755     685    2906

  Fragments arranged by size: 

                2906     755     685     603     258
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5207
 
PvuII CAG'CTG

Cuts at:   1751    2632    2992    1751 
   Size:        881     360    3966

  Fragments arranged by size: 

                3966     881     360
 
RsrII CG'GwC_CG

Cuts at:   3402    3402 
   Size:       5207
 
SacI G_AGCT'C

Cuts at:   2221    2221 
   Size:       5207
 
SacII CC_GC'GG

Cuts at:    193     211     193 
   Size:         18    5189
 
SalI G'TCGA_C

Cuts at:   2244    2244 
   Size:       5207
 
SapI GCTCTTCn'nnn_

Cuts at:   3230    3440    3230 
   Size:        210    4997
 
SbfI CC_TGCA'GG

Cuts at:   2254    2254 
   Size:       5207
 
ScaI AGT'ACT

Cuts at:   4724    4724 
   Size:       5207
 
SexAI A'CCwGG_T

Cuts at:   1295    1941    1295 
   Size:        646    4561
 
SfcI C'TryA_G

Cuts at:     33     634    1237    1495    2250    2935    3903    4094      33 
   Size:        601     603     258     755     685     968     191    1146

  Fragments arranged by size: 

                1146     968     755     685     603     601     258     191
            
 
SfoI GGC'GCC

Cuts at:   2639    2887    2639 
   Size:        248    4959
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5207
 
SmaI CCC'GGG

Cuts at:    143    2025    2229     143 
   Size:       1882     204    3121

  Fragments arranged by size: 

                3121    1882     204
 
SmlI C'TyrA_G

Cuts at:   1165    2362    3277    3991    4253    1165 
   Size:       1197     915     714     262    2119

  Fragments arranged by size: 

                2119    1197     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5207
 
SphI G_CATG'C

Cuts at:   2260    2760    2260 
   Size:        500    4707
 
SspI AAT'ATT

Cuts at:   1328    1328 
   Size:       5207
 
StuI AGG'CCT

Cuts at:   1962    1962 
   Size:       5207
 
StyI C'CwwG_G

Cuts at:    107     771    1577    1908    2341    2695     107 
   Size:        664     806     331     433     354    2619

  Fragments arranged by size: 

                2619     806     664     433     354     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    288     608    1460    1520    1931    1939    2790    2923    3382 
   Size:        320     852      60     411       8     851     133     459

Cuts at:   3382    3591     288 
   Size:        209    1904

  Fragments arranged by size: 

                1904     852     851     459     411     320     209     133
                  60       8
 
TatI w'GTAC_w

Cuts at:   1073    2141    4722    1073 
   Size:       1068    2581    1558

  Fragments arranged by size: 

                2581    1558    1068
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    280     312     767     799    4724    4756     280 
   Size:         32     455      32    3925      32     731

  Fragments arranged by size: 

                3925     731     455      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1297    3004    1297 
   Size:       1707    3500
 
XbaI T'CTAG_A

Cuts at:   2238    2238 
   Size:       5207
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1260    1691    2014    2702    1260 
   Size:        431     323     688    3765

  Fragments arranged by size: 

                3765     688     431     323
 
XmaI C'CCGG_G

Cuts at:    141    2023    2227     141 
   Size:       1882     204    3121

  Fragments arranged by size: 

                3121    1882     204
 
XmnI GAAnn'nnTTC

Cuts at:   1411    1411 
   Size:       5207
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AlfI     AloI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI   Bpu10I
    BsiWI     BsmI    BsmBI    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PsiI    PspXI     PsrI
    SanDI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI     ZraI
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