(Circular) (Six-base) MAPSORT of: pF1KB6014.seq Check: 2141 from: 1 to: 5207 pF1KB6014 5207 bp With 182 enzymes: SgfI * April 24, 2009 13:05 .. AccI GT'mk_AC Cuts at: 2245 2245 Size: 5207 Acc65I G'GTAC_C Cuts at: 2223 2223 Size: 5207 AclI AA'CG_TT Cuts at: 5047 5047 Size: 5207 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 857 1177 1675 3032 3464 3817 4479 857 Size: 320 498 1357 432 353 662 1585 Fragments arranged by size: 1585 1357 662 498 432 353 320 AflIII A'CryG_T Cuts at: 1814 2559 4359 4640 1814 Size: 745 1800 281 2381 Fragments arranged by size: 2381 1800 745 281 AhdI GACnn_n'nnGTC Cuts at: 1089 1089 Size: 5207 AlwNI CAG_nnn'CTG Cuts at: 2013 2512 3950 2013 Size: 499 1438 3270 Fragments arranged by size: 3270 1438 499 ApaI G_GGCC'C Cuts at: 417 1957 2024 2031 417 Size: 1540 67 7 3593 Fragments arranged by size: 3593 1540 67 7 ApaLI G'TGCA_C Cuts at: 1946 4045 1946 Size: 2099 3108 ApoI r'AATT_y Cuts at: 576 1029 1054 2211 4560 4816 576 Size: 453 25 1157 2349 256 967 Fragments arranged by size: 2349 1157 967 453 256 25 AseI AT'TA_AT Cuts at: 20 20 Size: 5207 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5207 AvaI C'yCGr_G Cuts at: 141 824 2023 2062 2227 141 Size: 683 1199 39 165 3121 Fragments arranged by size: 3121 1199 683 165 39 BamHI G'GATC_C Cuts at: 1271 1671 2232 1271 Size: 400 561 4246 Fragments arranged by size: 4246 561 400 BanI G'GyrC_C Cuts at: 2223 2637 2885 2920 2223 Size: 414 248 35 4510 Fragments arranged by size: 4510 414 248 35 BanII G_rGCy'C Cuts at: 174 417 1957 2024 2031 2221 3251 174 Size: 243 1540 67 7 190 1030 2130 Fragments arranged by size: 2130 1540 1030 243 190 67 7 BbeI G_GCGC'C Cuts at: 2641 2889 2641 Size: 248 4959 BbsI GAAGACnn'nnnn_ Cuts at: 1165 1481 1165 Size: 316 4891 BciVI GTATCCnnnnn_n' Cuts at: 3105 4161 5207 3105 Size: 1056 1046 3105 Fragments arranged by size: 3105 1056 1046 BclI T'GATC_A Cuts at: 2727 2727 Size: 5207 BglI GCCn_nnn'nGGC Cuts at: 112 124 408 1655 112 Size: 12 284 1247 3664 Fragments arranged by size: 3664 1247 284 12 BglII A'GATC_T Cuts at: 2722 4759 2722 Size: 2037 3170 BlpI GC'TnA_GC Cuts at: 2319 2319 Size: 5207 Bme1580I G_kGCm'C Cuts at: 417 1950 1957 2024 2031 2832 2925 4049 417 Size: 1533 7 67 7 801 93 1124 1575 Fragments arranged by size: 1575 1533 1124 801 93 67 7 7 BmrI ACTGGGnnnn_n' Cuts at: 447 689 1960 2419 2500 2835 4979 447 Size: 242 1271 459 81 335 2144 675 Fragments arranged by size: 2144 1271 675 459 335 242 81 BmtI G_CTAG'C Cuts at: 229 229 Size: 5207 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3574 3574 Size: 5207 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 590 874 1108 1990 2061 590 Size: 284 234 882 71 3736 Fragments arranged by size: 3736 882 284 234 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1150 2383 3262 3976 4274 1150 Size: 1233 879 714 298 2083 Fragments arranged by size: 2083 1233 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 1907 4911 1907 Size: 3004 2203 BsaAI yAC'GTr Cuts at: 738 1767 1817 2112 2560 738 Size: 1029 50 295 448 3385 Fragments arranged by size: 3385 1029 448 295 50 BsaBI GATnn'nnATC Cuts at: 6 2721 4758 6 Size: 2715 2037 455 Fragments arranged by size: 2715 2037 455 BsaHI Gr'CG_yC Cuts at: 290 2638 2886 3588 5084 290 Size: 2348 248 702 1496 413 Fragments arranged by size: 2348 1496 702 413 248 BsaWI w'CCGG_w Cuts at: 533 1704 2396 2917 3595 4006 4153 4427 533 Size: 1171 692 521 678 411 147 274 1313 Fragments arranged by size: 1313 1171 692 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1091 1121 1091 Size: 30 5177 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1521 1891 1521 Size: 370 4837 BseYI C'CCAG_C Cuts at: 421 1716 1751 2632 3390 4055 4463 421 Size: 1295 35 881 758 665 408 1165 Fragments arranged by size: 1295 1165 881 758 665 408 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 379 379 Size: 5207 BsiEI CG_ry'CG Cuts at: 104 119 347 1210 1647 2198 2749 2795 4025 Size: 15 228 863 437 551 551 46 1230 Cuts at: 4025 104 Size: 1286 Fragments arranged by size: 1286 1230 863 551 551 437 228 46 15 BsiHKAI G_wGCw'C Cuts at: 831 1553 1772 1950 2221 2999 3189 4049 831 Size: 722 219 178 271 778 190 860 1989 Fragments arranged by size: 1989 860 778 722 271 219 190 178 Bsp1286I G_dGCh'C Cuts at: 174 417 831 1553 1772 1950 1957 2024 2031 Size: 243 414 722 219 178 7 67 7 Cuts at: 2031 2221 2832 2925 2999 3189 3251 4049 174 Size: 190 611 93 74 190 62 798 1332 Fragments arranged by size: 1332 798 722 611 414 243 219 190 190 178 93 74 67 62 7 7 BspEI T'CCGG_A Cuts at: 1704 1704 Size: 5207 BspHI T'CATG_A Cuts at: 3639 3639 Size: 5207 BspMI ACCTGCnnnn'nnnn_ Cuts at: 168 2257 2471 2773 3154 168 Size: 2089 214 302 381 2221 Fragments arranged by size: 2221 2089 381 302 214 BsrBI CCG'CTC Cuts at: 50 154 3499 3553 5037 5202 50 Size: 104 3345 54 1484 165 55 Fragments arranged by size: 3345 1484 165 104 55 54 BsrDI GCAATG_nn' Cuts at: 970 3119 970 Size: 2149 3058 BsrFI r'CCGG_y Cuts at: 656 3205 3386 656 Size: 2549 181 2477 Fragments arranged by size: 2549 2477 181 BsrGI T'GTAC_A Cuts at: 1073 1073 Size: 5207 BssHII G'CGCG_C Cuts at: 133 133 Size: 5207 BssSI C'ACGA_G Cuts at: 355 3478 4186 355 Size: 3123 708 1376 Fragments arranged by size: 3123 1376 708 BstAPI GCAn_nnn'nTGC Cuts at: 2584 2584 Size: 5207 BstBI TT'CG_AA Cuts at: 3568 3568 Size: 5207 BstEII G'GTnAC_C Cuts at: 819 1387 819 Size: 568 4639 BstYI r'GATC_y Cuts at: 1 1271 1671 2232 2699 2722 3057 3303 3609 Size: 1270 400 561 467 23 335 246 306 Cuts at: 3609 3621 3707 4759 1 Size: 12 86 1052 449 Fragments arranged by size: 1270 1052 561 467 449 400 335 306 246 86 23 12 BtgI C'CryG_G Cuts at: 107 190 208 723 1577 1908 2198 107 Size: 83 18 515 854 331 290 3116 Fragments arranged by size: 3116 854 515 331 290 83 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 3157 3338 3501 3157 Size: 181 163 4863 Fragments arranged by size: 4863 181 163 BtsI GCAGTG_nn' Cuts at: 384 2450 384 Size: 2066 3141 DraI TTT'AAA Cuts at: 543 582 2206 543 Size: 39 1624 3544 Fragments arranged by size: 3544 1624 39 DraIII CAC_nnn'GTG Cuts at: 1945 1945 Size: 5207 DrdI GACnn_nn'nnGTC Cuts at: 2913 4257 2913 Size: 1344 3863 EaeI y'GGCC_r Cuts at: 116 344 381 1142 1187 1207 1250 1644 1745 Size: 228 37 761 45 20 43 394 101 Cuts at: 1745 1794 1859 2195 2792 2966 3357 3384 4729 Size: 49 65 336 597 174 391 27 1345 Cuts at: 4729 116 Size: 594 Fragments arranged by size: 1345 761 597 594 394 391 336 228 174 101 65 49 45 43 37 27 20 EagI C'GGCC_G Cuts at: 116 344 1207 1644 2195 2792 116 Size: 228 863 437 551 597 2531 Fragments arranged by size: 2531 863 597 551 437 228 EarI CTCTTCn'nnn_ Cuts at: 473 1868 3230 3440 473 Size: 1395 1362 210 2240 Fragments arranged by size: 2240 1395 1362 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3415 4157 4303 5017 3415 Size: 742 146 714 3605 Fragments arranged by size: 3605 742 714 146 EcoICRI GAG'CTC Cuts at: 2219 2219 Size: 5207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 590 857 874 1108 1177 1675 1990 2061 3032 Size: 267 17 234 69 498 315 71 971 Cuts at: 3032 3464 3817 4479 590 Size: 432 353 662 1318 Fragments arranged by size: 1318 971 662 498 432 353 315 267 234 71 69 17 EcoO109I rG'GnC_Cy Cuts at: 157 414 586 1740 1954 2027 2346 157 Size: 257 172 1154 214 73 319 3018 Fragments arranged by size: 3018 1154 319 257 214 172 73 EcoRI G'AATT_C Cuts at: 2211 2211 Size: 5207 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3226 3226 Size: 5207 FspI TGC'GCA Cuts at: 2988 2988 Size: 5207 HaeII r_GCGC'y Cuts at: 180 2641 2889 4119 4890 180 Size: 2461 248 1230 771 497 Fragments arranged by size: 2461 1230 771 497 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1075 1107 1656 1688 1920 1952 1968 2000 3049 Size: 32 549 32 232 32 16 32 1049 Cuts at: 3049 3079 3081 3111 1075 Size: 30 2 30 3171 Fragments arranged by size: 3171 1049 549 232 32 32 32 32 30 30 16 2 HincII GTy'rAC Cuts at: 1302 2070 2246 1302 Size: 768 176 4263 Fragments arranged by size: 4263 768 176 HindIII A'AGCT_T Cuts at: 1687 1687 Size: 5207 KasI G'GCGC_C Cuts at: 2637 2885 2637 Size: 248 4959 KpnI G_GTAC'C Cuts at: 2227 2227 Size: 5207 MluI A'CGCG_T Cuts at: 4640 4640 Size: 5207 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1208 1335 3966 4150 1208 Size: 127 2631 184 2265 Fragments arranged by size: 2631 2265 184 127 MscI TGG'CCA Cuts at: 383 1189 1252 1747 1861 2968 383 Size: 806 63 495 114 1107 2622 Fragments arranged by size: 2622 1107 806 495 114 63 MslI CAynn'nnrTG Cuts at: 817 1448 1664 2137 3196 3323 817 Size: 631 216 473 1059 127 2701 Fragments arranged by size: 2701 1059 631 473 216 127 MspA1I CmG'CkG Cuts at: 192 210 311 1751 2318 2632 2992 3776 4021 Size: 18 101 1440 567 314 360 784 245 Cuts at: 4021 192 Size: 1378 Fragments arranged by size: 1440 1378 784 567 360 314 245 101 18 NaeI GCC'GGC Cuts at: 658 3388 658 Size: 2730 2477 NarI GG'CG_CC Cuts at: 2638 2886 2638 Size: 248 4959 NcoI C'CATG_G Cuts at: 107 1577 1908 107 Size: 1470 331 3406 Fragments arranged by size: 3406 1470 331 NgoMIV G'CCGG_C Cuts at: 656 3386 656 Size: 2730 2477 NheI G'CTAG_C Cuts at: 225 225 Size: 5207 NspI r_CATG'y Cuts at: 2260 2760 4363 2260 Size: 500 1603 3104 Fragments arranged by size: 3104 1603 500 PciI A'CATG_T Cuts at: 4359 4359 Size: 5207 PflMI CCAn_nnn'nTGG Cuts at: 738 1613 2013 2171 738 Size: 875 400 158 3774 Fragments arranged by size: 3774 875 400 158 PmeI GTTT'AAAC Cuts at: 2206 2206 Size: 5207 PmlI CAC'GTG Cuts at: 1767 1767 Size: 5207 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3653 3685 3653 Size: 32 5175 PpuMI rG'GwC_Cy Cuts at: 157 586 157 Size: 429 4778 PshAI GACnn'nnGTC Cuts at: 2090 2090 Size: 5207 PspOMI G'GGCC_C Cuts at: 413 1953 2020 2027 413 Size: 1540 67 7 3593 Fragments arranged by size: 3593 1540 67 7 PstI C_TGCA'G Cuts at: 638 1241 1499 2254 2939 638 Size: 603 258 755 685 2906 Fragments arranged by size: 2906 755 685 603 258 PvuI CG_AT'CG Cuts at: 104 104 Size: 5207 PvuII CAG'CTG Cuts at: 1751 2632 2992 1751 Size: 881 360 3966 Fragments arranged by size: 3966 881 360 RsrII CG'GwC_CG Cuts at: 3402 3402 Size: 5207 SacI G_AGCT'C Cuts at: 2221 2221 Size: 5207 SacII CC_GC'GG Cuts at: 193 211 193 Size: 18 5189 SalI G'TCGA_C Cuts at: 2244 2244 Size: 5207 SapI GCTCTTCn'nnn_ Cuts at: 3230 3440 3230 Size: 210 4997 SbfI CC_TGCA'GG Cuts at: 2254 2254 Size: 5207 ScaI AGT'ACT Cuts at: 4724 4724 Size: 5207 SexAI A'CCwGG_T Cuts at: 1295 1941 1295 Size: 646 4561 SfcI C'TryA_G Cuts at: 33 634 1237 1495 2250 2935 3903 4094 33 Size: 601 603 258 755 685 968 191 1146 Fragments arranged by size: 1146 968 755 685 603 601 258 191 SfoI GGC'GCC Cuts at: 2639 2887 2639 Size: 248 4959 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5207 SmaI CCC'GGG Cuts at: 143 2025 2229 143 Size: 1882 204 3121 Fragments arranged by size: 3121 1882 204 SmlI C'TyrA_G Cuts at: 1165 2362 3277 3991 4253 1165 Size: 1197 915 714 262 2119 Fragments arranged by size: 2119 1197 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 5207 SphI G_CATG'C Cuts at: 2260 2760 2260 Size: 500 4707 SspI AAT'ATT Cuts at: 1328 1328 Size: 5207 StuI AGG'CCT Cuts at: 1962 1962 Size: 5207 StyI C'CwwG_G Cuts at: 107 771 1577 1908 2341 2695 107 Size: 664 806 331 433 354 2619 Fragments arranged by size: 2619 806 664 433 354 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 288 608 1460 1520 1931 1939 2790 2923 3382 Size: 320 852 60 411 8 851 133 459 Cuts at: 3382 3591 288 Size: 209 1904 Fragments arranged by size: 1904 852 851 459 411 320 209 133 60 8 TatI w'GTAC_w Cuts at: 1073 2141 4722 1073 Size: 1068 2581 1558 Fragments arranged by size: 2581 1558 1068 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 280 312 767 799 4724 4756 280 Size: 32 455 32 3925 32 731 Fragments arranged by size: 3925 731 455 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1297 3004 1297 Size: 1707 3500 XbaI T'CTAG_A Cuts at: 2238 2238 Size: 5207 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1260 1691 2014 2702 1260 Size: 431 323 688 3765 Fragments arranged by size: 3765 688 431 323 XmaI C'CCGG_G Cuts at: 141 2023 2227 141 Size: 1882 204 3121 Fragments arranged by size: 3121 1882 204 XmnI GAAnn'nnTTC Cuts at: 1411 1411 Size: 5207 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AgeI AleI AlfI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI Bpu10I BsiWI BsmI BsmBI BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NotI NruI NsiI PacI PasI PfoI PsiI PspXI PsrI SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI