Restriction map for
pF1KB6014
(Circular) (Six-base) MAPSORT of: pF1KB6014.seq Check: 2141 from: 1 to: 5207
pF1KB6014 5207 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 2245 2245
Size: 5207
Acc65I G'GTAC_C
Cuts at: 2223 2223
Size: 5207
AclI AA'CG_TT
Cuts at: 5047 5047
Size: 5207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 857 1177 1675 3032 3464 3817 4479 857
Size: 320 498 1357 432 353 662 1585
Fragments arranged by size:
1585 1357 662 498 432 353 320
AflIII A'CryG_T
Cuts at: 1814 2559 4359 4640 1814
Size: 745 1800 281 2381
Fragments arranged by size:
2381 1800 745 281
AhdI GACnn_n'nnGTC
Cuts at: 1089 1089
Size: 5207
AlwNI CAG_nnn'CTG
Cuts at: 2013 2512 3950 2013
Size: 499 1438 3270
Fragments arranged by size:
3270 1438 499
ApaI G_GGCC'C
Cuts at: 417 1957 2024 2031 417
Size: 1540 67 7 3593
Fragments arranged by size:
3593 1540 67 7
ApaLI G'TGCA_C
Cuts at: 1946 4045 1946
Size: 2099 3108
ApoI r'AATT_y
Cuts at: 576 1029 1054 2211 4560 4816 576
Size: 453 25 1157 2349 256 967
Fragments arranged by size:
2349 1157 967 453 256 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 5207
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
AvaI C'yCGr_G
Cuts at: 141 824 2023 2062 2227 141
Size: 683 1199 39 165 3121
Fragments arranged by size:
3121 1199 683 165 39
BamHI G'GATC_C
Cuts at: 1271 1671 2232 1271
Size: 400 561 4246
Fragments arranged by size:
4246 561 400
BanI G'GyrC_C
Cuts at: 2223 2637 2885 2920 2223
Size: 414 248 35 4510
Fragments arranged by size:
4510 414 248 35
BanII G_rGCy'C
Cuts at: 174 417 1957 2024 2031 2221 3251 174
Size: 243 1540 67 7 190 1030 2130
Fragments arranged by size:
2130 1540 1030 243 190 67 7
BbeI G_GCGC'C
Cuts at: 2641 2889 2641
Size: 248 4959
BbsI GAAGACnn'nnnn_
Cuts at: 1165 1481 1165
Size: 316 4891
BciVI GTATCCnnnnn_n'
Cuts at: 3105 4161 5207 3105
Size: 1056 1046 3105
Fragments arranged by size:
3105 1056 1046
BclI T'GATC_A
Cuts at: 2727 2727
Size: 5207
BglI GCCn_nnn'nGGC
Cuts at: 112 124 408 1655 112
Size: 12 284 1247 3664
Fragments arranged by size:
3664 1247 284 12
BglII A'GATC_T
Cuts at: 2722 4759 2722
Size: 2037 3170
BlpI GC'TnA_GC
Cuts at: 2319 2319
Size: 5207
Bme1580I G_kGCm'C
Cuts at: 417 1950 1957 2024 2031 2832 2925 4049 417
Size: 1533 7 67 7 801 93 1124 1575
Fragments arranged by size:
1575 1533 1124 801 93 67 7 7
BmrI ACTGGGnnnn_n'
Cuts at: 447 689 1960 2419 2500 2835 4979 447
Size: 242 1271 459 81 335 2144 675
Fragments arranged by size:
2144 1271 675 459 335 242 81
BmtI G_CTAG'C
Cuts at: 229 229
Size: 5207
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3574 3574
Size: 5207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 874 1108 1990 2061 590
Size: 284 234 882 71 3736
Fragments arranged by size:
3736 882 284 234 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2383 3262 3976 4274 1150
Size: 1233 879 714 298 2083
Fragments arranged by size:
2083 1233 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1907 4911 1907
Size: 3004 2203
BsaAI yAC'GTr
Cuts at: 738 1767 1817 2112 2560 738
Size: 1029 50 295 448 3385
Fragments arranged by size:
3385 1029 448 295 50
BsaBI GATnn'nnATC
Cuts at: 6 2721 4758 6
Size: 2715 2037 455
Fragments arranged by size:
2715 2037 455
BsaHI Gr'CG_yC
Cuts at: 290 2638 2886 3588 5084 290
Size: 2348 248 702 1496 413
Fragments arranged by size:
2348 1496 702 413 248
BsaWI w'CCGG_w
Cuts at: 533 1704 2396 2917 3595 4006 4153 4427 533
Size: 1171 692 521 678 411 147 274 1313
Fragments arranged by size:
1313 1171 692 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1091 1121 1091
Size: 30 5177
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1521 1891 1521
Size: 370 4837
BseYI C'CCAG_C
Cuts at: 421 1716 1751 2632 3390 4055 4463 421
Size: 1295 35 881 758 665 408 1165
Fragments arranged by size:
1295 1165 881 758 665 408 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 379 379
Size: 5207
BsiEI CG_ry'CG
Cuts at: 104 119 347 1210 1647 2198 2749 2795 4025
Size: 15 228 863 437 551 551 46 1230
Cuts at: 4025 104
Size: 1286
Fragments arranged by size:
1286 1230 863 551 551 437 228 46
15
BsiHKAI G_wGCw'C
Cuts at: 831 1553 1772 1950 2221 2999 3189 4049 831
Size: 722 219 178 271 778 190 860 1989
Fragments arranged by size:
1989 860 778 722 271 219 190 178
Bsp1286I G_dGCh'C
Cuts at: 174 417 831 1553 1772 1950 1957 2024 2031
Size: 243 414 722 219 178 7 67 7
Cuts at: 2031 2221 2832 2925 2999 3189 3251 4049 174
Size: 190 611 93 74 190 62 798 1332
Fragments arranged by size:
1332 798 722 611 414 243 219 190
190 178 93 74 67 62 7 7
BspEI T'CCGG_A
Cuts at: 1704 1704
Size: 5207
BspHI T'CATG_A
Cuts at: 3639 3639
Size: 5207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 2257 2471 2773 3154 168
Size: 2089 214 302 381 2221
Fragments arranged by size:
2221 2089 381 302 214
BsrBI CCG'CTC
Cuts at: 50 154 3499 3553 5037 5202 50
Size: 104 3345 54 1484 165 55
Fragments arranged by size:
3345 1484 165 104 55 54
BsrDI GCAATG_nn'
Cuts at: 970 3119 970
Size: 2149 3058
BsrFI r'CCGG_y
Cuts at: 656 3205 3386 656
Size: 2549 181 2477
Fragments arranged by size:
2549 2477 181
BsrGI T'GTAC_A
Cuts at: 1073 1073
Size: 5207
BssHII G'CGCG_C
Cuts at: 133 133
Size: 5207
BssSI C'ACGA_G
Cuts at: 355 3478 4186 355
Size: 3123 708 1376
Fragments arranged by size:
3123 1376 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2584 2584
Size: 5207
BstBI TT'CG_AA
Cuts at: 3568 3568
Size: 5207
BstEII G'GTnAC_C
Cuts at: 819 1387 819
Size: 568 4639
BstYI r'GATC_y
Cuts at: 1 1271 1671 2232 2699 2722 3057 3303 3609
Size: 1270 400 561 467 23 335 246 306
Cuts at: 3609 3621 3707 4759 1
Size: 12 86 1052 449
Fragments arranged by size:
1270 1052 561 467 449 400 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 190 208 723 1577 1908 2198 107
Size: 83 18 515 854 331 290 3116
Fragments arranged by size:
3116 854 515 331 290 83 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3157 3338 3501 3157
Size: 181 163 4863
Fragments arranged by size:
4863 181 163
BtsI GCAGTG_nn'
Cuts at: 384 2450 384
Size: 2066 3141
DraI TTT'AAA
Cuts at: 543 582 2206 543
Size: 39 1624 3544
Fragments arranged by size:
3544 1624 39
DraIII CAC_nnn'GTG
Cuts at: 1945 1945
Size: 5207
DrdI GACnn_nn'nnGTC
Cuts at: 2913 4257 2913
Size: 1344 3863
EaeI y'GGCC_r
Cuts at: 116 344 381 1142 1187 1207 1250 1644 1745
Size: 228 37 761 45 20 43 394 101
Cuts at: 1745 1794 1859 2195 2792 2966 3357 3384 4729
Size: 49 65 336 597 174 391 27 1345
Cuts at: 4729 116
Size: 594
Fragments arranged by size:
1345 761 597 594 394 391 336 228
174 101 65 49 45 43 37 27
20
EagI C'GGCC_G
Cuts at: 116 344 1207 1644 2195 2792 116
Size: 228 863 437 551 597 2531
Fragments arranged by size:
2531 863 597 551 437 228
EarI CTCTTCn'nnn_
Cuts at: 473 1868 3230 3440 473
Size: 1395 1362 210 2240
Fragments arranged by size:
2240 1395 1362 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3415 4157 4303 5017 3415
Size: 742 146 714 3605
Fragments arranged by size:
3605 742 714 146
EcoICRI GAG'CTC
Cuts at: 2219 2219
Size: 5207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 857 874 1108 1177 1675 1990 2061 3032
Size: 267 17 234 69 498 315 71 971
Cuts at: 3032 3464 3817 4479 590
Size: 432 353 662 1318
Fragments arranged by size:
1318 971 662 498 432 353 315 267
234 71 69 17
EcoO109I rG'GnC_Cy
Cuts at: 157 414 586 1740 1954 2027 2346 157
Size: 257 172 1154 214 73 319 3018
Fragments arranged by size:
3018 1154 319 257 214 172 73
EcoRI G'AATT_C
Cuts at: 2211 2211
Size: 5207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3226 3226
Size: 5207
FspI TGC'GCA
Cuts at: 2988 2988
Size: 5207
HaeII r_GCGC'y
Cuts at: 180 2641 2889 4119 4890 180
Size: 2461 248 1230 771 497
Fragments arranged by size:
2461 1230 771 497 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1075 1107 1656 1688 1920 1952 1968 2000 3049
Size: 32 549 32 232 32 16 32 1049
Cuts at: 3049 3079 3081 3111 1075
Size: 30 2 30 3171
Fragments arranged by size:
3171 1049 549 232 32 32 32 32
30 30 16 2
HincII GTy'rAC
Cuts at: 1302 2070 2246 1302
Size: 768 176 4263
Fragments arranged by size:
4263 768 176
HindIII A'AGCT_T
Cuts at: 1687 1687
Size: 5207
KasI G'GCGC_C
Cuts at: 2637 2885 2637
Size: 248 4959
KpnI G_GTAC'C
Cuts at: 2227 2227
Size: 5207
MluI A'CGCG_T
Cuts at: 4640 4640
Size: 5207
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 1335 3966 4150 1208
Size: 127 2631 184 2265
Fragments arranged by size:
2631 2265 184 127
MscI TGG'CCA
Cuts at: 383 1189 1252 1747 1861 2968 383
Size: 806 63 495 114 1107 2622
Fragments arranged by size:
2622 1107 806 495 114 63
MslI CAynn'nnrTG
Cuts at: 817 1448 1664 2137 3196 3323 817
Size: 631 216 473 1059 127 2701
Fragments arranged by size:
2701 1059 631 473 216 127
MspA1I CmG'CkG
Cuts at: 192 210 311 1751 2318 2632 2992 3776 4021
Size: 18 101 1440 567 314 360 784 245
Cuts at: 4021 192
Size: 1378
Fragments arranged by size:
1440 1378 784 567 360 314 245 101
18
NaeI GCC'GGC
Cuts at: 658 3388 658
Size: 2730 2477
NarI GG'CG_CC
Cuts at: 2638 2886 2638
Size: 248 4959
NcoI C'CATG_G
Cuts at: 107 1577 1908 107
Size: 1470 331 3406
Fragments arranged by size:
3406 1470 331
NgoMIV G'CCGG_C
Cuts at: 656 3386 656
Size: 2730 2477
NheI G'CTAG_C
Cuts at: 225 225
Size: 5207
NspI r_CATG'y
Cuts at: 2260 2760 4363 2260
Size: 500 1603 3104
Fragments arranged by size:
3104 1603 500
PciI A'CATG_T
Cuts at: 4359 4359
Size: 5207
PflMI CCAn_nnn'nTGG
Cuts at: 738 1613 2013 2171 738
Size: 875 400 158 3774
Fragments arranged by size:
3774 875 400 158
PmeI GTTT'AAAC
Cuts at: 2206 2206
Size: 5207
PmlI CAC'GTG
Cuts at: 1767 1767
Size: 5207
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 5175
PpuMI rG'GwC_Cy
Cuts at: 157 586 157
Size: 429 4778
PshAI GACnn'nnGTC
Cuts at: 2090 2090
Size: 5207
PspOMI G'GGCC_C
Cuts at: 413 1953 2020 2027 413
Size: 1540 67 7 3593
Fragments arranged by size:
3593 1540 67 7
PstI C_TGCA'G
Cuts at: 638 1241 1499 2254 2939 638
Size: 603 258 755 685 2906
Fragments arranged by size:
2906 755 685 603 258
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5207
PvuII CAG'CTG
Cuts at: 1751 2632 2992 1751
Size: 881 360 3966
Fragments arranged by size:
3966 881 360
RsrII CG'GwC_CG
Cuts at: 3402 3402
Size: 5207
SacI G_AGCT'C
Cuts at: 2221 2221
Size: 5207
SacII CC_GC'GG
Cuts at: 193 211 193
Size: 18 5189
SalI G'TCGA_C
Cuts at: 2244 2244
Size: 5207
SapI GCTCTTCn'nnn_
Cuts at: 3230 3440 3230
Size: 210 4997
SbfI CC_TGCA'GG
Cuts at: 2254 2254
Size: 5207
ScaI AGT'ACT
Cuts at: 4724 4724
Size: 5207
SexAI A'CCwGG_T
Cuts at: 1295 1941 1295
Size: 646 4561
SfcI C'TryA_G
Cuts at: 33 634 1237 1495 2250 2935 3903 4094 33
Size: 601 603 258 755 685 968 191 1146
Fragments arranged by size:
1146 968 755 685 603 601 258 191
SfoI GGC'GCC
Cuts at: 2639 2887 2639
Size: 248 4959
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5207
SmaI CCC'GGG
Cuts at: 143 2025 2229 143
Size: 1882 204 3121
Fragments arranged by size:
3121 1882 204
SmlI C'TyrA_G
Cuts at: 1165 2362 3277 3991 4253 1165
Size: 1197 915 714 262 2119
Fragments arranged by size:
2119 1197 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5207
SphI G_CATG'C
Cuts at: 2260 2760 2260
Size: 500 4707
SspI AAT'ATT
Cuts at: 1328 1328
Size: 5207
StuI AGG'CCT
Cuts at: 1962 1962
Size: 5207
StyI C'CwwG_G
Cuts at: 107 771 1577 1908 2341 2695 107
Size: 664 806 331 433 354 2619
Fragments arranged by size:
2619 806 664 433 354 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 608 1460 1520 1931 1939 2790 2923 3382
Size: 320 852 60 411 8 851 133 459
Cuts at: 3382 3591 288
Size: 209 1904
Fragments arranged by size:
1904 852 851 459 411 320 209 133
60 8
TatI w'GTAC_w
Cuts at: 1073 2141 4722 1073
Size: 1068 2581 1558
Fragments arranged by size:
2581 1558 1068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 280 312 767 799 4724 4756 280
Size: 32 455 32 3925 32 731
Fragments arranged by size:
3925 731 455 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1297 3004 1297
Size: 1707 3500
XbaI T'CTAG_A
Cuts at: 2238 2238
Size: 5207
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1260 1691 2014 2702 1260
Size: 431 323 688 3765
Fragments arranged by size:
3765 688 431 323
XmaI C'CCGG_G
Cuts at: 141 2023 2227 141
Size: 1882 204 3121
Fragments arranged by size:
3121 1882 204
XmnI GAAnn'nnTTC
Cuts at: 1411 1411
Size: 5207
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI Bpu10I
BsiWI BsmI BsmBI BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NotI
NruI NsiI PacI PasI PfoI PsiI PspXI PsrI
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI