Restriction map for
pF1KB6016
(Circular) (Six-base) MAPSORT of: pF1KB6016.seq Check: 2449 from: 1 to: 5936
pF1KB6016 5936 bp
With 182 enzymes: SgfI *
October 2, 2008 10:57 ..
AccI GT'mk_AC
Cuts at: 2974 2974
Size: 5936
Acc65I G'GTAC_C
Cuts at: 980 2952 980
Size: 1972 3964
AclI AA'CG_TT
Cuts at: 657 5776 657
Size: 5119 817
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 2162 2519 2650 3761 4193 4940 5208 956
Size: 1206 357 131 1111 432 747 268 1684
Fragments arranged by size:
1684 1206 1111 747 432 357 268 131
AflII C'TTAA_G
Cuts at: 2893 2893
Size: 5936
AflIII A'CryG_T
Cuts at: 2350 3288 4392 5369 2350
Size: 938 1104 977 2917
Fragments arranged by size:
2917 1104 977 938
AgeI A'CCGG_T
Cuts at: 4324 4324
Size: 5936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1904 1904
Size: 5936
AlwNI CAG_nnn'CTG
Cuts at: 148 1810 2420 3241 4808 148
Size: 1662 610 821 1567 1276
Fragments arranged by size:
1662 1567 1276 821 610
ApaLI G'TGCA_C
Cuts at: 2112 4706 2112
Size: 2594 3342
ApoI r'AATT_y
Cuts at: 793 1035 1291 1469 1568 1774 2258 2688 2940
Size: 242 256 178 99 206 484 430 252
Cuts at: 2940 5289 5545 793
Size: 2349 256 1184
Fragments arranged by size:
2349 1184 484 430 256 256 252 242
206 178 99
AseI AT'TA_AT
Cuts at: 20 20
Size: 5936
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
AvaI C'yCGr_G
Cuts at: 491 2107 2956 491
Size: 1616 849 3471
Fragments arranged by size:
3471 1616 849
BamHI G'GATC_C
Cuts at: 1163 2409 2961 1163
Size: 1246 552 4138
Fragments arranged by size:
4138 1246 552
BanI G'GyrC_C
Cuts at: 198 980 2772 2952 3366 3614 3649 198
Size: 782 1792 180 414 248 35 2485
Fragments arranged by size:
2485 1792 782 414 248 180 35
BanII G_rGCy'C
Cuts at: 1697 1759 2950 3980 1697
Size: 62 1191 1030 3653
Fragments arranged by size:
3653 1191 1030 62
BbeI G_GCGC'C
Cuts at: 202 3370 3618 202
Size: 3168 248 2520
Fragments arranged by size:
3168 2520 248
BbsI GAAGACnn'nnnn_
Cuts at: 489 2102 489
Size: 1613 4323
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 152 186 2098 2132 2437 2471 152
Size: 34 1912 34 305 34 3617
Fragments arranged by size:
3617 1912 305 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2476 3834 4595 5936 2476
Size: 1358 761 1341 2476
Fragments arranged by size:
2476 1358 1341 761
BclI T'GATC_A
Cuts at: 782 3456 782
Size: 2674 3262
BfrBI ATG'CAT
Cuts at: 531 2588 531
Size: 2057 3879
BglI GCCn_nnn'nGGC
Cuts at: 118 160 118
Size: 42 5894
BglII A'GATC_T
Cuts at: 1281 3451 5488 1281
Size: 2170 2037 1729
Fragments arranged by size:
2170 2037 1729
BlpI GC'TnA_GC
Cuts at: 3048 3048
Size: 5936
Bme1580I G_kGCm'C
Cuts at: 2116 2777 3561 3654 4710 2116
Size: 661 784 93 1056 3342
Fragments arranged by size:
3342 1056 784 661 93
BmgBI CAC'GTC
Cuts at: 249 249
Size: 5936
BmrI ACTGGGnnnn_n'
Cuts at: 3148 3229 3564 5708 3148
Size: 81 335 2144 3376
Fragments arranged by size:
3376 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4303 4303
Size: 5936
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 580 2389 170
Size: 410 1809 3717
Fragments arranged by size:
3717 1809 410
Bpu10I CC'TnA_GC
Cuts at: 1243 1243
Size: 5936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1773 2532 2643 3112 3991 4483 4781 1773
Size: 759 111 469 879 492 298 2928
Fragments arranged by size:
2928 879 759 492 469 298 111
BsaI GGTCTCn'nnnn_
Cuts at: 2787 5640 2787
Size: 2853 3083
BsaAI yAC'GTr
Cuts at: 2351 3289 2351
Size: 938 4998
BsaBI GATnn'nnATC
Cuts at: 6 1832 3450 5487 6
Size: 1826 1618 2037 455
Fragments arranged by size:
2037 1826 1618 455
BsaHI Gr'CG_yC
Cuts at: 199 3367 3615 4317 5813 199
Size: 3168 248 702 1496 322
Fragments arranged by size:
3168 1496 702 322 248
BsaWI w'CCGG_w
Cuts at: 2101 3125 3646 4324 4598 4745 5156 2101
Size: 1024 521 678 274 147 411 2881
Fragments arranged by size:
2881 1024 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 605 635 1085 1115 605
Size: 30 450 30 5426
Fragments arranged by size:
5426 450 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1549 1549
Size: 5936
BseYI C'CCAG_C
Cuts at: 2413 3361 4119 4696 5192 2413
Size: 948 758 577 496 3157
Fragments arranged by size:
3157 948 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 383 1201 2102 383
Size: 818 901 4217
Fragments arranged by size:
4217 901 818
BsiEI CG_ry'CG
Cuts at: 104 189 3478 3524 4732 104
Size: 85 3289 46 1208 1308
Fragments arranged by size:
3289 1308 1208 85 46
BsiHKAI G_wGCw'C
Cuts at: 1178 1697 1759 2116 2296 2950 3728 3918 4710
Size: 519 62 357 180 654 778 190 792
Cuts at: 4710 1178
Size: 2404
Fragments arranged by size:
2404 792 778 654 519 357 190 180
62
BsiWI C'GTAC_G
Cuts at: 242 242
Size: 5936
Bsp1286I G_dGCh'C
Cuts at: 1178 1697 1759 2116 2296 2777 2950 3561 3654
Size: 519 62 357 180 481 173 611 93
Cuts at: 3654 3728 3918 3980 4710 1178
Size: 74 190 62 730 2404
Fragments arranged by size:
2404 730 611 519 481 357 190 180
173 93 74 62 62
BspEI T'CCGG_A
Cuts at: 2101 5156 2101
Size: 3055 2881
BspHI T'CATG_A
Cuts at: 260 749 5112 260
Size: 489 4363 1084
Fragments arranged by size:
4363 1084 489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2986 3200 3502 3883 2986
Size: 214 302 381 5039
Fragments arranged by size:
5039 381 302 214
BsrBI CCG'CTC
Cuts at: 50 118 4228 4282 5766 5931 50
Size: 68 4110 54 1484 165 55
Fragments arranged by size:
4110 1484 165 68 55 54
BsrDI GCAATG_nn'
Cuts at: 3848 3848
Size: 5936
BsrFI r'CCGG_y
Cuts at: 233 2796 3934 4115 4324 233
Size: 2563 1138 181 209 1845
Fragments arranged by size:
2563 1845 1138 209 181
BssSI C'ACGA_G
Cuts at: 4207 4565 4207
Size: 358 5578
BstAPI GCAn_nnn'nTGC
Cuts at: 2381 3313 2381
Size: 932 5004
BstBI TT'CG_AA
Cuts at: 4297 4297
Size: 5936
BstYI r'GATC_y
Cuts at: 1 1163 1281 2409 2502 2565 2888 2961 3428
Size: 1162 118 1128 93 63 323 73 467
Cuts at: 3428 3451 3786 4032 5044 5130 5142 5488 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1162 1128 1012 467 449 346 335 323
246 118 93 86 73 63 23 12
Bsu36I CC'TnA_GG
Cuts at: 2561 2561
Size: 5936
BtgI C'CryG_G
Cuts at: 107 134 158 294 1735 2424 107
Size: 27 24 136 1441 689 3619
Fragments arranged by size:
3619 1441 689 136 27 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3886 4067 4230 3886
Size: 181 163 5592
Fragments arranged by size:
5592 181 163
BtsI GCAGTG_nn'
Cuts at: 1408 2114 2391 3179 1408
Size: 706 277 788 4165
Fragments arranged by size:
4165 788 706 277
DraI TTT'AAA
Cuts at: 1424 2935 1424
Size: 1511 4425
DrdI GACnn_nn'nnGTC
Cuts at: 3642 4500 3642
Size: 858 5078
EaeI y'GGCC_r
Cuts at: 110 131 186 871 2910 3521 3695 4086 4113
Size: 21 55 685 2039 611 174 391 27
Cuts at: 4113 5458 110
Size: 1345 588
Fragments arranged by size:
2039 1345 685 611 588 391 174 55
27 21
EagI C'GGCC_G
Cuts at: 186 3521 186
Size: 3335 2601
EarI CTCTTCn'nnn_
Cuts at: 1736 1922 1964 2138 3959 4169 1736
Size: 186 42 174 1821 210 3503
Fragments arranged by size:
3503 1821 210 186 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 194 4144 4454 4600 5746 194
Size: 3950 310 146 1146 384
Fragments arranged by size:
3950 1146 384 310 146
EcoICRI GAG'CTC
Cuts at: 1695 1757 2948 1695
Size: 62 1191 4683
Fragments arranged by size:
4683 1191 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 580 956 2162 2389 2519 2650 3761 4193
Size: 410 376 1206 227 130 131 1111 432
Cuts at: 4193 4940 5208 170
Size: 747 268 898
Fragments arranged by size:
1206 1111 898 747 432 410 376 268
227 131 130
EcoNI CCTnn'n_nnAGG
Cuts at: 1393 1393
Size: 5936
EcoO109I rG'GnC_Cy
Cuts at: 828 1710 3075 828
Size: 882 1365 3689
Fragments arranged by size:
3689 1365 882
EcoRI G'AATT_C
Cuts at: 1568 2688 2940 1568
Size: 1120 252 4564
Fragments arranged by size:
4564 1120 252
EcoRV GAT'ATC
Cuts at: 276 556 276
Size: 280 5656
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3955 3955
Size: 5936
FspI TGC'GCA
Cuts at: 3717 3717
Size: 5936
HaeII r_GCGC'y
Cuts at: 202 3370 3618 4640 5619 202
Size: 3168 248 1022 979 519
Fragments arranged by size:
3168 1022 979 519 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 563 595 605 637 2474 2487 2506 2519 3778
Size: 32 10 32 1837 13 19 13 1259
Cuts at: 3778 3808 3810 3840 563
Size: 30 2 30 2659
Fragments arranged by size:
2659 1837 1259 32 32 30 30 19
13 13 10 2
HincII GTy'rAC
Cuts at: 333 2975 333
Size: 2642 3294
KasI G'GCGC_C
Cuts at: 198 3366 3614 198
Size: 3168 248 2520
Fragments arranged by size:
3168 2520 248
KpnI G_GTAC'C
Cuts at: 984 2956 984
Size: 1972 3964
MluI A'CGCG_T
Cuts at: 5369 5369
Size: 5936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 1815 2213 4607 4791 288
Size: 1527 398 2394 184 1433
Fragments arranged by size:
2394 1527 1433 398 184
MscI TGG'CCA
Cuts at: 873 2912 3697 873
Size: 2039 785 3112
Fragments arranged by size:
3112 2039 785
MslI CAynn'nnrTG
Cuts at: 413 694 734 1234 3925 4052 413
Size: 281 40 500 2691 127 2297
Fragments arranged by size:
2691 2297 500 281 127 40
MspA1I CmG'CkG
Cuts at: 136 148 160 302 543 2420 2654 3047 3361
Size: 12 12 142 241 1877 234 393 314
Cuts at: 3361 3721 4734 4979 136
Size: 360 1013 245 1093
Fragments arranged by size:
1877 1093 1013 393 360 314 245 241
234 142 12 12
NaeI GCC'GGC
Cuts at: 235 4117 235
Size: 3882 2054
NarI GG'CG_CC
Cuts at: 199 3367 3615 199
Size: 3168 248 2520
Fragments arranged by size:
3168 2520 248
NcoI C'CATG_G
Cuts at: 107 1735 2424 107
Size: 1628 689 3619
Fragments arranged by size:
3619 1628 689
NdeI CA'TA_TG
Cuts at: 1312 1312
Size: 5936
NgoMIV G'CCGG_C
Cuts at: 233 4115 233
Size: 3882 2054
NsiI A_TGCA'T
Cuts at: 533 2590 533
Size: 2057 3879
NspI r_CATG'y
Cuts at: 1239 1462 2322 2989 3489 4396 1239
Size: 223 860 667 500 907 2779
Fragments arranged by size:
2779 907 860 667 500 223
PciI A'CATG_T
Cuts at: 4392 4392
Size: 5936
PflMI CCAn_nnn'nTGG
Cuts at: 1316 2023 1316
Size: 707 5229
PfoI T'CCnGG_A
Cuts at: 2405 2524 2405
Size: 119 5817
PmeI GTTT'AAAC
Cuts at: 2935 2935
Size: 5936
PmlI CAC'GTG
Cuts at: 2351 2351
Size: 5936
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5075 5107 5075
Size: 32 5904
PpuMI rG'GwC_Cy
Cuts at: 828 1710 828
Size: 882 5054
PsiI TTA'TAA
Cuts at: 523 523
Size: 5936
PstI C_TGCA'G
Cuts at: 2386 2983 3668 2386
Size: 597 685 4654
Fragments arranged by size:
4654 685 597
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5936
PvuII CAG'CTG
Cuts at: 148 302 543 2420 3361 3721 148
Size: 154 241 1877 941 360 2363
Fragments arranged by size:
2363 1877 941 360 241 154
RsrII CG'GwC_CG
Cuts at: 4131 4131
Size: 5936
SacI G_AGCT'C
Cuts at: 1697 1759 2950 1697
Size: 62 1191 4683
Fragments arranged by size:
4683 1191 62
SacII CC_GC'GG
Cuts at: 137 161 137
Size: 24 5912
SalI G'TCGA_C
Cuts at: 2973 2973
Size: 5936
SapI GCTCTTCn'nnn_
Cuts at: 3959 4169 3959
Size: 210 5726
SbfI CC_TGCA'GG
Cuts at: 2983 2983
Size: 5936
ScaI AGT'ACT
Cuts at: 5453 5453
Size: 5936
SfcI C'TryA_G
Cuts at: 33 1154 2382 2979 3664 4657 4848 33
Size: 1121 1228 597 685 993 191 1121
Fragments arranged by size:
1228 1121 1121 993 685 597 191
SfoI GGC'GCC
Cuts at: 200 3368 3616 200
Size: 3168 248 2520
Fragments arranged by size:
3168 2520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5936
SmaI CCC'GGG
Cuts at: 2958 2958
Size: 5936
SmlI C'TyrA_G
Cuts at: 491 1752 2511 2658 2893 3091 4006 4498 4760
Size: 1261 759 147 235 198 915 492 262
Cuts at: 4760 491
Size: 1667
Fragments arranged by size:
1667 1261 915 759 492 262 235 198
147
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5936
SphI G_CATG'C
Cuts at: 1239 2322 2989 3489 1239
Size: 1083 667 500 3686
Fragments arranged by size:
3686 1083 667 500
SspI AAT'ATT
Cuts at: 913 913
Size: 5936
StyI C'CwwG_G
Cuts at: 107 1735 2424 3070 3424 107
Size: 1628 689 646 354 2619
Fragments arranged by size:
2619 1628 689 646 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 846 3519 3652 4111 4320 846
Size: 2673 133 459 209 2462
Fragments arranged by size:
2673 2462 459 209 133
TatI w'GTAC_w
Cuts at: 221 1392 5451 221
Size: 1171 4059 706
Fragments arranged by size:
4059 1171 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1085 1117 5453 5485 1085
Size: 32 4336 32 1536
Fragments arranged by size:
4336 1536 32 32
Tth111I GACn'n_nGTC
Cuts at: 3733 3733
Size: 5936
XbaI T'CTAG_A
Cuts at: 2248 2967 2248
Size: 719 5217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1335 2722 3431 1335
Size: 1387 709 3840
Fragments arranged by size:
3840 1387 709
XhoI C'TCGA_G
Cuts at: 491 491
Size: 5936
XmaI C'CCGG_G
Cuts at: 2956 2956
Size: 5936
XmnI GAAnn'nnTTC
Cuts at: 256 1671 2309 256
Size: 1415 638 3883
Fragments arranged by size:
3883 1415 638
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BmtI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII FseI FspAI
HindIII HpaI MfeI NheI NotI NruI PacI PasI
PshAI PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI ZraI