Restriction map for
pF1KB6017
(Circular) (Six-base) MAPSORT of: pF1KB6017.seq Check: 8188 from: 1 to: 5594
pF1KB6017 5594 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2237 2237
Size: 5594
AccI GT'mk_AC
Cuts at: 1544 2632 1544
Size: 1088 4506
Acc65I G'GTAC_C
Cuts at: 887 2610 887
Size: 1723 3871
AclI AA'CG_TT
Cuts at: 5434 5434
Size: 5594
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1842 2388 2404 2512 3419 3851 4598 4866 1842
Size: 546 16 108 907 432 747 268 2570
Fragments arranged by size:
2570 907 747 546 432 268 108 16
AfeI AGC'GCT
Cuts at: 507 507
Size: 5594
AflIII A'CryG_T
Cuts at: 2531 2946 4050 5027 2531
Size: 415 1104 977 3098
Fragments arranged by size:
3098 1104 977 415
AgeI A'CCGG_T
Cuts at: 3982 3982
Size: 5594
AlwNI CAG_nnn'CTG
Cuts at: 301 1107 2899 4466 301
Size: 806 1792 1567 1429
Fragments arranged by size:
1792 1567 1429 806
ApaI G_GGCC'C
Cuts at: 1476 1476
Size: 5594
ApaLI G'TGCA_C
Cuts at: 1552 4364 1552
Size: 2812 2782
ApoI r'AATT_y
Cuts at: 2598 4947 5203 2598
Size: 2349 256 2989
Fragments arranged by size:
2989 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5594
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5594
AvaI C'yCGr_G
Cuts at: 940 952 1263 1926 2614 940
Size: 12 311 663 688 3920
Fragments arranged by size:
3920 688 663 311 12
BamHI G'GATC_C
Cuts at: 1178 2619 1178
Size: 1441 4153
BanI G'GyrC_C
Cuts at: 307 382 539 619 760 887 1042 1849 2610
Size: 75 157 80 141 127 155 807 761
Cuts at: 2610 3024 3272 3307 307
Size: 414 248 35 2594
Fragments arranged by size:
2594 807 761 414 248 157 155 141
127 80 75 35
BanII G_rGCy'C
Cuts at: 190 739 1476 1799 1870 2423 2608 3638 190
Size: 549 737 323 71 553 185 1030 2146
Fragments arranged by size:
2146 1030 737 553 549 323 185 71
BbeI G_GCGC'C
Cuts at: 543 3028 3276 543
Size: 2485 248 2861
Fragments arranged by size:
2861 2485 248
BbsI GAAGACnn'nnnn_
Cuts at: 384 2022 2101 2190 384
Size: 1638 79 89 3788
Fragments arranged by size:
3788 1638 89 79
BbvCI CC'TCA_GC
Cuts at: 1037 1037
Size: 5594
BciVI GTATCCnnnnn_n'
Cuts at: 3492 4253 5594 3492
Size: 761 1341 3492
Fragments arranged by size:
3492 1341 761
BclI T'GATC_A
Cuts at: 3114 3114
Size: 5594
BglI GCCn_nnn'nGGC
Cuts at: 734 919 1480 734
Size: 185 561 4848
Fragments arranged by size:
4848 561 185
BglII A'GATC_T
Cuts at: 680 3109 5146 680
Size: 2429 2037 1128
Fragments arranged by size:
2429 2037 1128
BlpI GC'TnA_GC
Cuts at: 460 2473 2706 460
Size: 2013 233 3348
Fragments arranged by size:
3348 2013 233
Bme1580I G_kGCm'C
Cuts at: 1476 1556 3219 3312 4368 1476
Size: 80 1663 93 1056 2702
Fragments arranged by size:
2702 1663 1056 93 80
BmgBI CAC'GTC
Cuts at: 1611 1611
Size: 5594
BmrI ACTGGGnnnn_n'
Cuts at: 2806 2887 3222 5366 2806
Size: 81 335 2144 3034
Fragments arranged by size:
3034 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3961 3961
Size: 5594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1699 2566 676
Size: 1023 867 3704
Fragments arranged by size:
3704 1023 867
Bpu10I CC'TnA_GC
Cuts at: 1037 1183 2569 1037
Size: 146 1386 4062
Fragments arranged by size:
4062 1386 146
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 2407 2770 3649 4141 4439 198
Size: 2209 363 879 492 298 1353
Fragments arranged by size:
2209 1353 879 492 363 298
BsaI GGTCTCn'nnnn_
Cuts at: 154 1951 5298 154
Size: 1797 3347 450
Fragments arranged by size:
3347 1797 450
BsaAI yAC'GTr
Cuts at: 1083 1143 1548 2532 2553 2947 1083
Size: 60 405 984 21 394 3730
Fragments arranged by size:
3730 984 405 394 60 21
BsaBI GATnn'nnATC
Cuts at: 6 3108 5145 6
Size: 3102 2037 455
Fragments arranged by size:
3102 2037 455
BsaHI Gr'CG_yC
Cuts at: 540 1159 2018 2195 3025 3273 3975 5471 540
Size: 619 859 177 830 248 702 1496 663
Fragments arranged by size:
1496 859 830 702 663 619 248 177
BsaWI w'CCGG_w
Cuts at: 1175 2261 2783 3304 3982 4256 4403 4814 1175
Size: 1086 522 521 678 274 147 411 1955
Fragments arranged by size:
1955 1086 678 522 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2149 2179 2452 2482 2149
Size: 30 273 30 5261
Fragments arranged by size:
5261 273 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 673 1399 1807 673
Size: 726 408 4460
Fragments arranged by size:
4460 726 408
BseYI C'CCAG_C
Cuts at: 191 223 327 483 962 987 1107 1134 1361
Size: 32 104 156 479 25 120 27 227
Cuts at: 1361 1644 1821 2280 3019 3777 4354 4850 191
Size: 283 177 459 739 758 577 496 935
Fragments arranged by size:
935 758 739 577 496 479 459 283
227 177 156 120 104 32 27 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 890 1165 1324 1342 890
Size: 275 159 18 5142
Fragments arranged by size:
5142 275 159 18
BsiEI CG_ry'CG
Cuts at: 104 1176 1499 3136 3182 4390 104
Size: 1072 323 1637 46 1208 1308
Fragments arranged by size:
1637 1308 1208 1072 323 46
BsiHKAI G_wGCw'C
Cuts at: 1556 1799 2078 2423 2447 2552 2608 3386 3576
Size: 243 279 345 24 105 56 778 190
Cuts at: 3576 4368 1556
Size: 792 2782
Fragments arranged by size:
2782 792 778 345 279 243 190 105
56 24
BsmI GAATG_Cn'
Cuts at: 1497 1497
Size: 5594
BsmBI CGTCTCn'nnnn_
Cuts at: 471 471
Size: 5594
Bsp1286I G_dGCh'C
Cuts at: 190 739 1476 1556 1799 1870 2078 2423 2447
Size: 549 737 80 243 71 208 345 24
Cuts at: 2447 2552 2608 3219 3312 3386 3576 3638 4368
Size: 105 56 611 93 74 190 62 730
Cuts at: 4368 190
Size: 1416
Fragments arranged by size:
1416 737 730 611 549 345 243 208
190 105 93 80 74 71 62 56
24
BspEI T'CCGG_A
Cuts at: 4814 4814
Size: 5594
BspHI T'CATG_A
Cuts at: 4770 4770
Size: 5594
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2237 2644 2858 3160 3541 2237
Size: 407 214 302 381 4290
Fragments arranged by size:
4290 407 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3886 3940 5424 5589 50
Size: 3836 54 1484 165 55
Fragments arranged by size:
3836 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 3506 2000
Size: 1506 4088
BsrFI r'CCGG_y
Cuts at: 875 1242 1519 1615 3592 3773 3982 875
Size: 367 277 96 1977 181 209 2487
Fragments arranged by size:
2487 1977 367 277 209 181 96
BsrGI T'GTAC_A
Cuts at: 2165 2165
Size: 5594
BssSI C'ACGA_G
Cuts at: 140 421 3865 4223 140
Size: 281 3444 358 1511
Fragments arranged by size:
3444 1511 358 281
BstAPI GCAn_nnn'nTGC
Cuts at: 2451 2971 2451
Size: 520 5074
BstBI TT'CG_AA
Cuts at: 1565 3955 1565
Size: 2390 3204
BstEII G'GTnAC_C
Cuts at: 1254 1254
Size: 5594
BstXI CCAn_nnnn'nTGG
Cuts at: 1369 2220 1369
Size: 851 4743
BstYI r'GATC_y
Cuts at: 1 680 1178 2619 3086 3109 3444 3690 4702
Size: 679 498 1441 467 23 335 246 1012
Cuts at: 4702 4788 4800 5146 1
Size: 86 12 346 449
Fragments arranged by size:
1441 1012 679 498 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 1545 1545
Size: 5594
Bsu36I CC'TnA_GG
Cuts at: 915 915
Size: 5594
BtgI C'CryG_G
Cuts at: 1005 1331 1368 1530 1892 1961 2153 2455 1005
Size: 326 37 162 362 69 192 302 4144
Fragments arranged by size:
4144 362 326 302 192 162 69 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 705 1017 3544 3725 3888 705
Size: 312 2527 181 163 2411
Fragments arranged by size:
2527 2411 312 181 163
BtsI GCAGTG_nn'
Cuts at: 243 830 879 1874 2470 2837 243
Size: 587 49 995 596 367 3000
Fragments arranged by size:
3000 995 596 587 367 49
ClaI AT'CG_AT
Cuts at: 743 815 743
Size: 72 5522
DraI TTT'AAA
Cuts at: 2593 2593
Size: 5594
DraIII CAC_nnn'GTG
Cuts at: 1611 1611
Size: 5594
DrdI GACnn_nn'nnGTC
Cuts at: 2062 3300 4158 2062
Size: 1238 858 3498
Fragments arranged by size:
3498 1238 858
EaeI y'GGCC_r
Cuts at: 1454 1496 1613 1985 2522 3179 3353 3744 3771
Size: 42 117 372 537 657 174 391 27
Cuts at: 3771 5116 1454
Size: 1345 1932
Fragments arranged by size:
1932 1345 657 537 391 372 174 117
42 27
EagI C'GGCC_G
Cuts at: 1496 3179 1496
Size: 1683 3911
EarI CTCTTCn'nnn_
Cuts at: 3617 3827 3617
Size: 210 5384
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 685 3802 4112 4258 5404 685
Size: 3117 310 146 1146 875
Fragments arranged by size:
3117 1146 875 310 146
EcoICRI GAG'CTC
Cuts at: 1797 2421 2606 1797
Size: 624 185 4785
Fragments arranged by size:
4785 624 185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1699 1842 2388 2404 2512 2566 3419 3851
Size: 1023 143 546 16 108 54 853 432
Cuts at: 3851 4598 4866 676
Size: 747 268 1404
Fragments arranged by size:
1404 1023 853 747 546 432 268 143
108 54 16
EcoNI CCTnn'n_nnAGG
Cuts at: 424 655 1576 1666 2233 424
Size: 231 921 90 567 3785
Fragments arranged by size:
3785 921 567 231 90
EcoO109I rG'GnC_Cy
Cuts at: 216 417 851 1010 1089 1472 1473 1658 2037
Size: 201 434 159 79 383 1 185 379
Cuts at: 2037 2112 2733 216
Size: 75 621 3077
Fragments arranged by size:
3077 621 434 383 379 201 185 159
79 75 1
EcoRI G'AATT_C
Cuts at: 2598 2598
Size: 5594
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1461 2214 3613 1461
Size: 753 1399 3442
Fragments arranged by size:
3442 1399 753
FspI TGC'GCA
Cuts at: 3375 3375
Size: 5594
HaeII r_GCGC'y
Cuts at: 509 543 898 1219 1788 1941 2246 3028 3276
Size: 34 355 321 569 153 305 782 248
Cuts at: 3276 4298 5277 509
Size: 1022 979 826
Fragments arranged by size:
1022 979 826 782 569 355 321 305
248 153 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1007 1039 3436 3466 3468 3498 1007
Size: 32 2397 30 2 30 3103
Fragments arranged by size:
3103 2397 32 30 30 2
HincII GTy'rAC
Cuts at: 2067 2633 2067
Size: 566 5028
KasI G'GCGC_C
Cuts at: 539 3024 3272 539
Size: 2485 248 2861
Fragments arranged by size:
2861 2485 248
KpnI G_GTAC'C
Cuts at: 891 2614 891
Size: 1723 3871
MluI A'CGCG_T
Cuts at: 5027 5027
Size: 5594
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 494 1895 2272 2288 4265 4449 494
Size: 1401 377 16 1977 184 1639
Fragments arranged by size:
1977 1639 1401 377 184 16
MscI TGG'CCA
Cuts at: 1456 1987 3355 1456
Size: 531 1368 3695
Fragments arranged by size:
3695 1368 531
MslI CAynn'nnrTG
Cuts at: 376 766 1807 2005 2518 3583 3710 376
Size: 390 1041 198 513 1065 127 2260
Fragments arranged by size:
2260 1065 1041 513 390 198 127
MspA1I CmG'CkG
Cuts at: 223 483 550 1149 1677 1767 2705 3019 3379
Size: 260 67 599 528 90 938 314 360
Cuts at: 3379 4392 4637 223
Size: 1013 245 1180
Fragments arranged by size:
1180 1013 938 599 528 360 314 260
245 90 67
NaeI GCC'GGC
Cuts at: 877 1244 1617 3775 877
Size: 367 373 2158 2696
Fragments arranged by size:
2696 2158 373 367
NarI GG'CG_CC
Cuts at: 540 3025 3273 540
Size: 2485 248 2861
Fragments arranged by size:
2861 2485 248
NcoI C'CATG_G
Cuts at: 1331 1530 1892 1961 1331
Size: 199 362 69 4964
Fragments arranged by size:
4964 362 199 69
NgoMIV G'CCGG_C
Cuts at: 875 1242 1615 3773 875
Size: 367 373 2158 2696
Fragments arranged by size:
2696 2158 373 367
NspI r_CATG'y
Cuts at: 2454 2647 3147 4054 2454
Size: 193 500 907 3994
Fragments arranged by size:
3994 907 500 193
PciI A'CATG_T
Cuts at: 4050 4050
Size: 5594
PflMI CCAn_nnn'nTGG
Cuts at: 2300 2300
Size: 5594
PmeI GTTT'AAAC
Cuts at: 2593 2593
Size: 5594
PmlI CAC'GTG
Cuts at: 1143 2553 1143
Size: 1410 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 4733
Size: 32 5562
PpuMI rG'GwC_Cy
Cuts at: 851 1010 1089 1658 2037 851
Size: 159 79 569 379 4408
Fragments arranged by size:
4408 569 379 159 79
PshAI GACnn'nnGTC
Cuts at: 2215 2215
Size: 5594
PspOMI G'GGCC_C
Cuts at: 1472 1472
Size: 5594
PstI C_TGCA'G
Cuts at: 250 1766 2209 2234 2641 3326 250
Size: 1516 443 25 407 685 2518
Fragments arranged by size:
2518 1516 685 443 407 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5594
PvuII CAG'CTG
Cuts at: 483 1677 1767 3019 3379 483
Size: 1194 90 1252 360 2698
Fragments arranged by size:
2698 1252 1194 360 90
RsrII CG'GwC_CG
Cuts at: 1517 3789 1517
Size: 2272 3322
SacI G_AGCT'C
Cuts at: 1799 2423 2608 1799
Size: 624 185 4785
Fragments arranged by size:
4785 624 185
SalI G'TCGA_C
Cuts at: 2631 2631
Size: 5594
SanDI GG'GwC_CC
Cuts at: 1010 1010
Size: 5594
SapI GCTCTTCn'nnn_
Cuts at: 3617 3827 3617
Size: 210 5384
SbfI CC_TGCA'GG
Cuts at: 2641 2641
Size: 5594
ScaI AGT'ACT
Cuts at: 5111 5111
Size: 5594
SexAI A'CCwGG_T
Cuts at: 560 1091 560
Size: 531 5063
SfcI C'TryA_G
Cuts at: 33 246 692 1762 2205 2230 2637 3322 4315
Size: 213 446 1070 443 25 407 685 993
Cuts at: 4315 4506 33
Size: 191 1121
Fragments arranged by size:
1121 1070 993 685 446 443 407 213
191 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 919 919
Size: 5594
SfoI GGC'GCC
Cuts at: 541 3026 3274 541
Size: 2485 248 2861
Fragments arranged by size:
2861 2485 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5594
SmaI CCC'GGG
Cuts at: 2616 2616
Size: 5594
SmlI C'TyrA_G
Cuts at: 177 2422 2749 3664 4156 4418 177
Size: 2245 327 915 492 262 1353
Fragments arranged by size:
2245 1353 915 492 327 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5594
SphI G_CATG'C
Cuts at: 2454 2647 3147 2454
Size: 193 500 4901
Fragments arranged by size:
4901 500 193
StuI AGG'CCT
Cuts at: 520 1571 1747 520
Size: 1051 176 4367
Fragments arranged by size:
4367 1051 176
StyI C'CwwG_G
Cuts at: 212 1313 1331 1430 1530 1577 1667 1892 1961
Size: 1101 18 99 100 47 90 225 69
Cuts at: 1961 2728 3082 212
Size: 767 354 2724
Fragments arranged by size:
2724 1101 767 354 225 100 99 90
69 47 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 370 979 1375 3177 3310 3769 3978 370
Size: 609 396 1802 133 459 209 1986
Fragments arranged by size:
1986 1802 609 459 396 209 133
TatI w'GTAC_w
Cuts at: 2165 2174 5109 2165
Size: 9 2935 2650
Fragments arranged by size:
2935 2650 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2452 2484 5111 5143 2452
Size: 32 2627 32 2903
Fragments arranged by size:
2903 2627 32 32
Tth111I GACn'n_nGTC
Cuts at: 3391 3391
Size: 5594
XbaI T'CTAG_A
Cuts at: 2625 2625
Size: 5594
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 199 1369 1464 1674 2462 3089 199
Size: 1170 95 210 788 627 2704
Fragments arranged by size:
2704 1170 788 627 210 95
XmaI C'CCGG_G
Cuts at: 2614 2614
Size: 5594
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BssHII CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PsiI PspXI PsrI
SacII SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI