Restriction map for
pF1KB6103
(Circular) (Six-base) MAPSORT of: pF1KB6103.seq Check: 5680 from: 1 to: 4844
pF1KB6103 4844 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AccI GT'mk_AC
Cuts at: 1212 1882 1212
Size: 670 4174
Acc65I G'GTAC_C
Cuts at: 1860 1860
Size: 4844
AclI AA'CG_TT
Cuts at: 509 4684 509
Size: 4175 669
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 524 1493 2669 3101 3848 4116 422
Size: 102 969 1176 432 747 268 1150
Fragments arranged by size:
1176 1150 969 747 432 268 102
AflIII A'CryG_T
Cuts at: 2196 3300 4277 2196
Size: 1104 977 2763
Fragments arranged by size:
2763 1104 977
AgeI A'CCGG_T
Cuts at: 3232 3232
Size: 4844
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 323 355 323
Size: 32 4812
AlwNI CAG_nnn'CTG
Cuts at: 769 2149 3716 769
Size: 1380 1567 1897
Fragments arranged by size:
1897 1567 1380
ApaI G_GGCC'C
Cuts at: 1710 1710
Size: 4844
ApaLI G'TGCA_C
Cuts at: 3614 3614
Size: 4844
ApoI r'AATT_y
Cuts at: 577 1164 1243 1577 1720 1848 4197 4453 577
Size: 587 79 334 143 128 2349 256 968
Fragments arranged by size:
2349 968 587 334 256 143 128 79
AseI AT'TA_AT
Cuts at: 20 616 1780 20
Size: 596 1164 3084
Fragments arranged by size:
3084 1164 596
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
AvaI C'yCGr_G
Cuts at: 1864 1864
Size: 4844
BamHI G'GATC_C
Cuts at: 1516 1869 1516
Size: 353 4491
BanI G'GyrC_C
Cuts at: 1860 2274 2522 2557 1860
Size: 414 248 35 4147
Fragments arranged by size:
4147 414 248 35
BanII G_rGCy'C
Cuts at: 1710 1858 2888 1710
Size: 148 1030 3666
Fragments arranged by size:
3666 1030 148
BbeI G_GCGC'C
Cuts at: 2278 2526 2278
Size: 248 4596
BbsI GAAGACnn'nnnn_
Cuts at: 137 137
Size: 4844
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 640 674 640
Size: 34 4810
BciVI GTATCCnnnnn_n'
Cuts at: 2742 3503 4844 2742
Size: 761 1341 2742
Fragments arranged by size:
2742 1341 761
BclI T'GATC_A
Cuts at: 2364 2364
Size: 4844
BglII A'GATC_T
Cuts at: 2359 4396 2359
Size: 2037 2807
BlpI GC'TnA_GC
Cuts at: 1956 1956
Size: 4844
Bme1580I G_kGCm'C
Cuts at: 1710 2469 2562 3618 1710
Size: 759 93 1056 2936
Fragments arranged by size:
2936 1056 759 93
BmrI ACTGGGnnnn_n'
Cuts at: 1704 2056 2137 2472 4616 1704
Size: 352 81 335 2144 1932
Fragments arranged by size:
2144 1932 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3211 3211
Size: 4844
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 163
Size: 4844
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2020 2899 3391 3689 2020
Size: 879 492 298 3175
Fragments arranged by size:
3175 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 635 4548 635
Size: 3913 931
BsaAI yAC'GTr
Cuts at: 2197 2197
Size: 4844
BsaBI GATnn'nnATC
Cuts at: 6 2358 4395 6
Size: 2352 2037 455
Fragments arranged by size:
2352 2037 455
BsaHI Gr'CG_yC
Cuts at: 2275 2523 3225 4721 2275
Size: 248 702 1496 2398
Fragments arranged by size:
2398 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2033 2554 3232 3506 3653 4064 2033
Size: 521 678 274 147 411 2813
Fragments arranged by size:
2813 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 215 1325 1385 1846 215
Size: 1110 60 461 3213
Fragments arranged by size:
3213 1110 461 60
BseYI C'CCAG_C
Cuts at: 2269 3027 3604 4100 2269
Size: 758 577 496 3013
Fragments arranged by size:
3013 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2386 2432 3640 104
Size: 2282 46 1208 1308
Fragments arranged by size:
2282 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1858 2636 2826 3618 1858
Size: 778 190 792 3084
Fragments arranged by size:
3084 792 778 190
BsmI GAATG_Cn'
Cuts at: 1675 1675
Size: 4844
Bsp1286I G_dGCh'C
Cuts at: 1710 1858 2469 2562 2636 2826 2888 3618 1710
Size: 148 611 93 74 190 62 730 2936
Fragments arranged by size:
2936 730 611 190 148 93 74 62
BspEI T'CCGG_A
Cuts at: 4064 4064
Size: 4844
BspHI T'CATG_A
Cuts at: 4020 4020
Size: 4844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1894 2108 2410 2791 1894
Size: 214 302 381 3947
Fragments arranged by size:
3947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3136 3190 4674 4839 50
Size: 3086 54 1484 165 55
Fragments arranged by size:
3086 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2756 2756
Size: 4844
BsrFI r'CCGG_y
Cuts at: 2842 3023 3232 2842
Size: 181 209 4454
Fragments arranged by size:
4454 209 181
BssSI C'ACGA_G
Cuts at: 3115 3473 3115
Size: 358 4486
BstAPI GCAn_nnn'nTGC
Cuts at: 921 2221 921
Size: 1300 3544
BstBI TT'CG_AA
Cuts at: 1086 3205 1086
Size: 2119 2725
BstYI r'GATC_y
Cuts at: 1 1516 1869 2336 2359 2694 2940 3952 4038
Size: 1515 353 467 23 335 246 1012 86
Cuts at: 4038 4050 4396 1
Size: 12 346 449
Fragments arranged by size:
1515 1012 467 449 353 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1213 1213
Size: 4844
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2794 2975 3138 2794
Size: 181 163 4500
Fragments arranged by size:
4500 181 163
BtsI GCAGTG_nn'
Cuts at: 880 1669 2087 880
Size: 789 418 3637
Fragments arranged by size:
3637 789 418
DraI TTT'AAA
Cuts at: 1248 1843 1248
Size: 595 4249
DraIII CAC_nnn'GTG
Cuts at: 156 156
Size: 4844
DrdI GACnn_nn'nnGTC
Cuts at: 295 2550 3408 295
Size: 2255 858 1731
Fragments arranged by size:
2255 1731 858
EaeI y'GGCC_r
Cuts at: 2429 2603 2994 3021 4366 2429
Size: 174 391 27 1345 2907
Fragments arranged by size:
2907 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2429 2429
Size: 4844
EarI CTCTTCn'nnn_
Cuts at: 327 698 1276 1312 1502 1823 2867 3077 327
Size: 371 578 36 190 321 1044 210 2094
Fragments arranged by size:
2094 1044 578 371 321 210 190 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3052 3362 3508 4654 3052
Size: 310 146 1146 3242
Fragments arranged by size:
3242 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1856 1856
Size: 4844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 422 524 1493 2669 3101 3848 4116 163
Size: 259 102 969 1176 432 747 268 891
Fragments arranged by size:
1176 969 891 747 432 268 259 102
EcoO109I rG'GnC_Cy
Cuts at: 1983 1983
Size: 4844
EcoRI G'AATT_C
Cuts at: 1164 1848 1164
Size: 684 4160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 4844
FspI TGC'GCA
Cuts at: 2625 2625
Size: 4844
HaeII r_GCGC'y
Cuts at: 2278 2526 3548 4527 2278
Size: 248 1022 979 2595
Fragments arranged by size:
2595 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 729 761 2686 2716 2718 2748 729
Size: 32 1925 30 2 30 2825
Fragments arranged by size:
2825 1925 32 30 30 2
HincII GTy'rAC
Cuts at: 240 587 1715 1883 240
Size: 347 1128 168 3201
Fragments arranged by size:
3201 1128 347 168
HindIII A'AGCT_T
Cuts at: 1735 1735
Size: 4844
HpaI GTT'AAC
Cuts at: 1715 1715
Size: 4844
KasI G'GCGC_C
Cuts at: 2274 2522 2274
Size: 248 4596
KpnI G_GTAC'C
Cuts at: 1864 1864
Size: 4844
MluI A'CGCG_T
Cuts at: 4277 4277
Size: 4844
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 3515 3699 689
Size: 2826 184 1834
Fragments arranged by size:
2826 1834 184
MscI TGG'CCA
Cuts at: 2605 2605
Size: 4844
MslI CAynn'nnrTG
Cuts at: 1388 2833 2960 1388
Size: 1445 127 3272
Fragments arranged by size:
3272 1445 127
MspA1I CmG'CkG
Cuts at: 1605 1955 2269 2629 3642 3887 1605
Size: 350 314 360 1013 245 2562
Fragments arranged by size:
2562 1013 360 350 314 245
NaeI GCC'GGC
Cuts at: 3025 3025
Size: 4844
NarI GG'CG_CC
Cuts at: 2275 2523 2275
Size: 248 4596
NdeI CA'TA_TG
Cuts at: 1193 1193
Size: 4844
NgoMIV G'CCGG_C
Cuts at: 3023 3023
Size: 4844
NspI r_CATG'y
Cuts at: 1897 2397 3304 1897
Size: 500 907 3437
Fragments arranged by size:
3437 907 500
PciI A'CATG_T
Cuts at: 3300 3300
Size: 4844
PflMI CCAn_nnn'nTGG
Cuts at: 1290 1290
Size: 4844
PfoI T'CCnGG_A
Cuts at: 1137 1137
Size: 4844
PmeI GTTT'AAAC
Cuts at: 1843 1843
Size: 4844
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 3983
Size: 32 4812
PsiI TTA'TAA
Cuts at: 1741 1741
Size: 4844
PspOMI G'GGCC_C
Cuts at: 1706 1706
Size: 4844
PstI C_TGCA'G
Cuts at: 887 1891 2576 887
Size: 1004 685 3155
Fragments arranged by size:
3155 1004 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4844
PvuII CAG'CTG
Cuts at: 1605 2269 2629 1605
Size: 664 360 3820
Fragments arranged by size:
3820 664 360
RsrII CG'GwC_CG
Cuts at: 3039 3039
Size: 4844
SacI G_AGCT'C
Cuts at: 1858 1858
Size: 4844
SalI G'TCGA_C
Cuts at: 1881 1881
Size: 4844
SapI GCTCTTCn'nnn_
Cuts at: 1312 2867 3077 1312
Size: 1555 210 3079
Fragments arranged by size:
3079 1555 210
SbfI CC_TGCA'GG
Cuts at: 1891 1891
Size: 4844
ScaI AGT'ACT
Cuts at: 4361 4361
Size: 4844
SfcI C'TryA_G
Cuts at: 33 368 883 1887 2572 3565 3756 33
Size: 335 515 1004 685 993 191 1121
Fragments arranged by size:
1121 1004 993 685 515 335 191
SfoI GGC'GCC
Cuts at: 2276 2524 2276
Size: 248 4596
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4844
SmaI CCC'GGG
Cuts at: 1866 1866
Size: 4844
SmlI C'TyrA_G
Cuts at: 1999 2914 3406 3668 1999
Size: 915 492 262 3175
Fragments arranged by size:
3175 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4844
SphI G_CATG'C
Cuts at: 1897 2397 1897
Size: 500 4344
SspI AAT'ATT
Cuts at: 655 835 655
Size: 180 4664
StyI C'CwwG_G
Cuts at: 625 1026 1978 2332 625
Size: 401 952 354 3137
Fragments arranged by size:
3137 952 401 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2427 2560 3019 3228 2427
Size: 133 459 209 4043
Fragments arranged by size:
4043 459 209 133
TatI w'GTAC_w
Cuts at: 4359 4359
Size: 4844
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1133 1165 4361 4393 1133
Size: 32 3196 32 1584
Fragments arranged by size:
3196 1584 32 32
Tth111I GACn'n_nGTC
Cuts at: 2641 2641
Size: 4844
XbaI T'CTAG_A
Cuts at: 186 1875 186
Size: 1689 3155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1330 2339 1330
Size: 1009 3835
XmaI C'CCGG_G
Cuts at: 1864 1864
Size: 4844
XmnI GAAnn'nnTTC
Cuts at: 1082 1082
Size: 4844
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
MfeI NcoI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI