Restriction map for
pF1KB6119
(Circular) (Six-base) MAPSORT of: pF1KB6119.seq Check: 1131 from: 1 to: 4982
pF1KB6119 4982 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 926 926
Size: 4982
AccI GT'mk_AC
Cuts at: 2020 2020
Size: 4982
Acc65I G'GTAC_C
Cuts at: 1998 1998
Size: 4982
AclI AA'CG_TT
Cuts at: 4822 4822
Size: 4982
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 2807 3239 3986 4254 161
Size: 2646 432 747 268 889
Fragments arranged by size:
2646 889 747 432 268
AflIII A'CryG_T
Cuts at: 120 2334 3438 4415 120
Size: 2214 1104 977 687
Fragments arranged by size:
2214 1104 977 687
AgeI A'CCGG_T
Cuts at: 3370 3370
Size: 4982
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1849 1881 1849
Size: 32 4950
AlwNI CAG_nnn'CTG
Cuts at: 2287 3854 2287
Size: 1567 3415
ApaLI G'TGCA_C
Cuts at: 3752 3752
Size: 4982
ApoI r'AATT_y
Cuts at: 183 242 307 788 892 1986 4335 4591 183
Size: 59 65 481 104 1094 2349 256 574
Fragments arranged by size:
2349 1094 574 481 256 104 65 59
AseI AT'TA_AT
Cuts at: 20 20
Size: 4982
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
AvaI C'yCGr_G
Cuts at: 2002 2002
Size: 4982
BamHI G'GATC_C
Cuts at: 693 2007 693
Size: 1314 3668
BanI G'GyrC_C
Cuts at: 1729 1838 1998 2412 2660 2695 1729
Size: 109 160 414 248 35 4016
Fragments arranged by size:
4016 414 248 160 109 35
BanII G_rGCy'C
Cuts at: 149 853 1867 1996 3026 149
Size: 704 1014 129 1030 2105
Fragments arranged by size:
2105 1030 1014 704 129
BbeI G_GCGC'C
Cuts at: 1842 2416 2664 1842
Size: 574 248 4160
Fragments arranged by size:
4160 574 248
BbsI GAAGACnn'nnnn_
Cuts at: 323 412 323
Size: 89 4893
BciVI GTATCCnnnnn_n'
Cuts at: 147 1947 2880 3641 4982 147
Size: 1800 933 761 1341 147
Fragments arranged by size:
1800 1341 933 761 147
BclI T'GATC_A
Cuts at: 1902 2502 1902
Size: 600 4382
BglII A'GATC_T
Cuts at: 621 2497 4534 621
Size: 1876 2037 1069
Fragments arranged by size:
2037 1876 1069
BlpI GC'TnA_GC
Cuts at: 1880 2094 1880
Size: 214 4768
Bme1580I G_kGCm'C
Cuts at: 1734 2607 2700 3756 1734
Size: 873 93 1056 2960
Fragments arranged by size:
2960 1056 873 93
BmrI ACTGGGnnnn_n'
Cuts at: 1869 2194 2275 2610 4754 1869
Size: 325 81 335 2144 2097
Fragments arranged by size:
2144 2097 335 325 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3349 3349
Size: 4982
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 1463 536
Size: 927 4055
Bpu10I CC'TnA_GC
Cuts at: 755 755
Size: 4982
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2158 3037 3529 3827 2158
Size: 879 492 298 3313
Fragments arranged by size:
3313 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 712 4686 712
Size: 3974 1008
BsaAI yAC'GTr
Cuts at: 1759 2335 1759
Size: 576 4406
BsaBI GATnn'nnATC
Cuts at: 6 1094 2496 4533 6
Size: 1088 1402 2037 455
Fragments arranged by size:
2037 1402 1088 455
BsaHI Gr'CG_yC
Cuts at: 923 1839 2413 2661 3363 4859 923
Size: 916 574 248 702 1496 1046
Fragments arranged by size:
1496 1046 916 702 574 248
BsaWI w'CCGG_w
Cuts at: 942 2171 2692 3370 3644 3791 4202 942
Size: 1229 521 678 274 147 411 1722
Fragments arranged by size:
1722 1229 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1849 1879 1849
Size: 30 4952
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1625 1643 1700 1739 1625
Size: 18 57 39 4868
Fragments arranged by size:
4868 57 39 18
BseYI C'CCAG_C
Cuts at: 2407 3165 3742 4238 2407
Size: 758 577 496 3151
Fragments arranged by size:
3151 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2524 2570 3778 104
Size: 2420 46 1208 1308
Fragments arranged by size:
2420 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1996 2774 2964 3756 1996
Size: 778 190 792 3222
Fragments arranged by size:
3222 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 149 853 1734 1867 1996 2607 2700 2774 2964
Size: 704 881 133 129 611 93 74 190
Cuts at: 2964 3026 3756 149
Size: 62 730 1375
Fragments arranged by size:
1375 881 730 704 611 190 133 129
93 74 62
BspEI T'CCGG_A
Cuts at: 4202 4202
Size: 4982
BspHI T'CATG_A
Cuts at: 401 783 4158 401
Size: 382 3375 1225
Fragments arranged by size:
3375 1225 382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2032 2246 2548 2929 2032
Size: 214 302 381 4085
Fragments arranged by size:
4085 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1890 3274 3328 4812 4977 50
Size: 1840 1384 54 1484 165 55
Fragments arranged by size:
1840 1484 1384 165 55 54
BsrDI GCAATG_nn'
Cuts at: 193 1874 2894 193
Size: 1681 1020 2281
Fragments arranged by size:
2281 1681 1020
BsrFI r'CCGG_y
Cuts at: 1669 2980 3161 3370 1669
Size: 1311 181 209 3281
Fragments arranged by size:
3281 1311 209 181
BsrGI T'GTAC_A
Cuts at: 739 739
Size: 4982
BssSI C'ACGA_G
Cuts at: 3253 3611 3253
Size: 358 4624
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 2359 1621
Size: 738 4244
BstBI TT'CG_AA
Cuts at: 3343 3343
Size: 4982
BstYI r'GATC_y
Cuts at: 1 611 621 675 693 1089 1554 2007 2474
Size: 610 10 54 18 396 465 453 467
Cuts at: 2474 2497 2832 3078 4090 4176 4188 4534 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 610 467 465 453 449 396 346
335 246 86 54 23 18 12 10
Bsu36I CC'TnA_GG
Cuts at: 1100 1100
Size: 4982
BtgI C'CryG_G
Cuts at: 107 149 1659 1749 1776 107
Size: 42 1510 90 27 3313
Fragments arranged by size:
3313 1510 90 42 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2932 3113 3276 2932
Size: 181 163 4638
Fragments arranged by size:
4638 181 163
BtsI GCAGTG_nn'
Cuts at: 2225 2225
Size: 4982
DraI TTT'AAA
Cuts at: 302 348 1981 302
Size: 46 1633 3303
Fragments arranged by size:
3303 1633 46
DraIII CAC_nnn'GTG
Cuts at: 271 271
Size: 4982
DrdI GACnn_nn'nnGTC
Cuts at: 2688 3546 2688
Size: 858 4124
EaeI y'GGCC_r
Cuts at: 1773 2567 2741 3132 3159 4504 1773
Size: 794 174 391 27 1345 2251
Fragments arranged by size:
2251 1345 794 391 174 27
EagI C'GGCC_G
Cuts at: 2567 2567
Size: 4982
EarI CTCTTCn'nnn_
Cuts at: 137 1154 3005 3215 137
Size: 1017 1851 210 1904
Fragments arranged by size:
1904 1851 1017 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3190 3500 3646 4792 3190
Size: 310 146 1146 3380
Fragments arranged by size:
3380 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1994 1994
Size: 4982
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 536 1463 2807 3239 3986 4254 161
Size: 375 927 1344 432 747 268 889
Fragments arranged by size:
1344 927 889 747 432 375 268
EcoO109I rG'GnC_Cy
Cuts at: 1690 2121 1690
Size: 431 4551
EcoRI G'AATT_C
Cuts at: 307 1986 307
Size: 1679 3303
EcoRV GAT'ATC
Cuts at: 1474 1474
Size: 4982
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 504 3001 504
Size: 2497 2485
FspI TGC'GCA
Cuts at: 2763 2763
Size: 4982
HaeII r_GCGC'y
Cuts at: 1842 2416 2664 3686 4665 1842
Size: 574 248 1022 979 2159
Fragments arranged by size:
2159 1022 979 574 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1674 1706 1836 1868 2824 2854 2856 2886 1674
Size: 32 130 32 956 30 2 30 3770
Fragments arranged by size:
3770 956 130 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2021 2021
Size: 4982
HindIII A'AGCT_T
Cuts at: 296 296
Size: 4982
KasI G'GCGC_C
Cuts at: 1838 2412 2660 1838
Size: 574 248 4160
Fragments arranged by size:
4160 574 248
KpnI G_GTAC'C
Cuts at: 2002 2002
Size: 4982
MluI A'CGCG_T
Cuts at: 4415 4415
Size: 4982
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1038 1456 1566 3653 3837 1038
Size: 418 110 2087 184 2183
Fragments arranged by size:
2183 2087 418 184 110
MscI TGG'CCA
Cuts at: 2743 2743
Size: 4982
MslI CAynn'nnrTG
Cuts at: 125 809 1241 2971 3098 125
Size: 684 432 1730 127 2009
Fragments arranged by size:
2009 1730 684 432 127
MspA1I CmG'CkG
Cuts at: 1003 1661 1751 1778 2093 2407 2767 3780 4025
Size: 658 90 27 315 314 360 1013 245
Cuts at: 4025 1003
Size: 1960
Fragments arranged by size:
1960 1013 658 360 315 314 245 90
27
NaeI GCC'GGC
Cuts at: 3163 3163
Size: 4982
NarI GG'CG_CC
Cuts at: 1839 2413 2661 1839
Size: 574 248 4160
Fragments arranged by size:
4160 574 248
NcoI C'CATG_G
Cuts at: 107 149 107
Size: 42 4940
NdeI CA'TA_TG
Cuts at: 1421 1421
Size: 4982
NgoMIV G'CCGG_C
Cuts at: 3161 3161
Size: 4982
NspI r_CATG'y
Cuts at: 124 2035 2535 3442 124
Size: 1911 500 907 1664
Fragments arranged by size:
1911 1664 907 500
PciI A'CATG_T
Cuts at: 120 3438 120
Size: 3318 1664
PflMI CCAn_nnn'nTGG
Cuts at: 703 1564 703
Size: 861 4121
PfoI T'CCnGG_A
Cuts at: 1692 1692
Size: 4982
PmeI GTTT'AAAC
Cuts at: 1981 1981
Size: 4982
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1849 1881 4121 4153 1849
Size: 32 2240 32 2678
Fragments arranged by size:
2678 2240 32 32
PpuMI rG'GwC_Cy
Cuts at: 1690 1690
Size: 4982
PstI C_TGCA'G
Cuts at: 168 2029 2714 168
Size: 1861 685 2436
Fragments arranged by size:
2436 1861 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4982
PvuII CAG'CTG
Cuts at: 1003 2407 2767 1003
Size: 1404 360 3218
Fragments arranged by size:
3218 1404 360
RsrII CG'GwC_CG
Cuts at: 3177 3177
Size: 4982
SacI G_AGCT'C
Cuts at: 1996 1996
Size: 4982
SacII CC_GC'GG
Cuts at: 1662 1752 1779 1662
Size: 90 27 4865
Fragments arranged by size:
4865 90 27
SalI G'TCGA_C
Cuts at: 2019 2019
Size: 4982
SapI GCTCTTCn'nnn_
Cuts at: 137 3005 3215 137
Size: 2868 210 1904
Fragments arranged by size:
2868 1904 210
SbfI CC_TGCA'GG
Cuts at: 2029 2029
Size: 4982
ScaI AGT'ACT
Cuts at: 4499 4499
Size: 4982
SfcI C'TryA_G
Cuts at: 33 113 164 1082 2025 2710 3703 3894 33
Size: 80 51 918 943 685 993 191 1121
Fragments arranged by size:
1121 993 943 918 685 191 80 51
SfoI GGC'GCC
Cuts at: 1840 2414 2662 1840
Size: 574 248 4160
Fragments arranged by size:
4160 574 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4982
SmaI CCC'GGG
Cuts at: 2004 2004
Size: 4982
SmlI C'TyrA_G
Cuts at: 2137 3052 3544 3806 2137
Size: 915 492 262 3313
Fragments arranged by size:
3313 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4982
SphI G_CATG'C
Cuts at: 2035 2535 2035
Size: 500 4482
SspI AAT'ATT
Cuts at: 1308 1308
Size: 4982
StyI C'CwwG_G
Cuts at: 107 149 872 1893 2116 2470 107
Size: 42 723 1021 223 354 2619
Fragments arranged by size:
2619 1021 723 354 223 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2565 2698 3157 3366 2565
Size: 133 459 209 4181
Fragments arranged by size:
4181 459 209 133
TatI w'GTAC_w
Cuts at: 739 4497 739
Size: 3758 1224
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4499 4531 4499
Size: 32 4950
Tth111I GACn'n_nGTC
Cuts at: 2779 2779
Size: 4982
XbaI T'CTAG_A
Cuts at: 2013 2013
Size: 4982
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1537 2477 1537
Size: 940 4042
XmaI C'CCGG_G
Cuts at: 2002 2002
Size: 4982
XmnI GAAnn'nnTTC
Cuts at: 185 641 881 1577 185
Size: 456 240 696 3590
Fragments arranged by size:
3590 696 456 240
ZraI GAC'GTC
Cuts at: 924 924
Size: 4982
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoNI FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PmlI PshAI PsiI
PspOMI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI