Restriction map for
pF1KB6120
(Circular) (Six-base) MAPSORT of: pF1KB6120.seq Check: 1902 from: 1 to: 4862
pF1KB6120 4862 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 257 1900 257
Size: 1643 3219
Acc65I G'GTAC_C
Cuts at: 1878 1878
Size: 4862
AclI AA'CG_TT
Cuts at: 4702 4702
Size: 4862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 703 1316 1610 1675 2687 3119 3866 4134
Size: 317 613 294 65 1012 432 747 268
Cuts at: 4134 386
Size: 1114
Fragments arranged by size:
1114 1012 747 613 432 317 294 268
65
AflIII A'CryG_T
Cuts at: 1647 2214 3318 4295 1647
Size: 567 1104 977 2214
Fragments arranged by size:
2214 1104 977 567
AgeI A'CCGG_T
Cuts at: 3250 3250
Size: 4862
AlwNI CAG_nnn'CTG
Cuts at: 469 2167 3734 469
Size: 1698 1567 1597
Fragments arranged by size:
1698 1597 1567
ApaLI G'TGCA_C
Cuts at: 3632 3632
Size: 4862
ApoI r'AATT_y
Cuts at: 157 355 868 1375 1866 4215 4471 157
Size: 198 513 507 491 2349 256 548
Fragments arranged by size:
2349 548 513 507 491 256 198
AseI AT'TA_AT
Cuts at: 20 385 20
Size: 365 4497
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4862
AvaI C'yCGr_G
Cuts at: 1882 1882
Size: 4862
BamHI G'GATC_C
Cuts at: 1887 1887
Size: 4862
BanI G'GyrC_C
Cuts at: 897 1878 2292 2540 2575 897
Size: 981 414 248 35 3184
Fragments arranged by size:
3184 981 414 248 35
BanII G_rGCy'C
Cuts at: 1876 2906 1876
Size: 1030 3832
BbeI G_GCGC'C
Cuts at: 2296 2544 2296
Size: 248 4614
BbsI GAAGACnn'nnnn_
Cuts at: 1475 1475
Size: 4862
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4828
BciVI GTATCCnnnnn_n'
Cuts at: 2760 3521 4862 2760
Size: 761 1341 2760
Fragments arranged by size:
2760 1341 761
BclI T'GATC_A
Cuts at: 2382 2382
Size: 4862
BglII A'GATC_T
Cuts at: 696 2377 4414 696
Size: 1681 2037 1144
Fragments arranged by size:
2037 1681 1144
BlpI GC'TnA_GC
Cuts at: 149 1974 149
Size: 1825 3037
Bme1580I G_kGCm'C
Cuts at: 1071 2487 2580 3636 1071
Size: 1416 93 1056 2297
Fragments arranged by size:
2297 1416 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2074 2155 2490 4634 2074
Size: 81 335 2144 2302
Fragments arranged by size:
2302 2144 335 81
BmtI G_CTAG'C
Cuts at: 1360 1360
Size: 4862
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3229 3229
Size: 4862
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 852 852
Size: 4862
Bpu10I CC'TnA_GC
Cuts at: 1028 1028
Size: 4862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2038 2917 3409 3707 2038
Size: 879 492 298 3193
Fragments arranged by size:
3193 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4566 4566
Size: 4862
BsaAI yAC'GTr
Cuts at: 2215 2215
Size: 4862
BsaBI GATnn'nnATC
Cuts at: 6 2376 4413 6
Size: 2370 2037 455
Fragments arranged by size:
2370 2037 455
BsaHI Gr'CG_yC
Cuts at: 2293 2541 3243 4739 2293
Size: 248 702 1496 2416
Fragments arranged by size:
2416 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2051 2572 3250 3524 3671 4082 2051
Size: 521 678 274 147 411 2831
Fragments arranged by size:
2831 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1054 1054
Size: 4862
BseYI C'CCAG_C
Cuts at: 569 2287 3045 3622 4118 569
Size: 1718 758 577 496 1313
Fragments arranged by size:
1718 1313 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2404 2450 3658 104
Size: 2300 46 1208 1308
Fragments arranged by size:
2300 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1876 2654 2844 3636 1876
Size: 778 190 792 3102
Fragments arranged by size:
3102 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1071 1876 2487 2580 2654 2844 2906 3636 1071
Size: 805 611 93 74 190 62 730 2297
Fragments arranged by size:
2297 805 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4082 4082
Size: 4862
BspHI T'CATG_A
Cuts at: 4038 4038
Size: 4862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1912 2126 2428 2809 1912
Size: 214 302 381 3965
Fragments arranged by size:
3965 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3154 3208 4692 4857 50
Size: 3104 54 1484 165 55
Fragments arranged by size:
3104 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1859 2774 1859
Size: 915 3947
BsrFI r'CCGG_y
Cuts at: 2860 3041 3250 2860
Size: 181 209 4472
Fragments arranged by size:
4472 209 181
BssSI C'ACGA_G
Cuts at: 3133 3491 3133
Size: 358 4504
BstAPI GCAn_nnn'nTGC
Cuts at: 1309 1369 2239 1309
Size: 60 870 3932
Fragments arranged by size:
3932 870 60
BstBI TT'CG_AA
Cuts at: 3223 3223
Size: 4862
BstYI r'GATC_y
Cuts at: 1 696 1887 2354 2377 2712 2958 3970 4056
Size: 695 1191 467 23 335 246 1012 86
Cuts at: 4056 4068 4414 1
Size: 12 346 449
Fragments arranged by size:
1191 1012 695 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2812 2993 3156 2812
Size: 181 163 4518
Fragments arranged by size:
4518 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 2105 1379
Size: 726 4136
ClaI AT'CG_AT
Cuts at: 1838 1838
Size: 4862
DraI TTT'AAA
Cuts at: 1861 1861
Size: 4862
DrdI GACnn_nn'nnGTC
Cuts at: 2568 3426 2568
Size: 858 4004
EaeI y'GGCC_r
Cuts at: 2447 2621 3012 3039 4384 2447
Size: 174 391 27 1345 2925
Fragments arranged by size:
2925 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2447 2447
Size: 4862
EarI CTCTTCn'nnn_
Cuts at: 415 1136 1166 1292 1418 1484 2885 3095 415
Size: 721 30 126 126 66 1401 210 2182
Fragments arranged by size:
2182 1401 721 210 126 126 66 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3070 3380 3526 4672 3070
Size: 310 146 1146 3260
Fragments arranged by size:
3260 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1874 1874
Size: 4862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 703 852 1316 1610 1675 2687 3119 3866
Size: 317 149 464 294 65 1012 432 747
Cuts at: 3866 4134 386
Size: 268 1114
Fragments arranged by size:
1114 1012 747 464 432 317 294 268
149 65
EcoO109I rG'GnC_Cy
Cuts at: 2001 2001
Size: 4862
EcoRI G'AATT_C
Cuts at: 1375 1866 1375
Size: 491 4371
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2881 2881
Size: 4862
FspI TGC'GCA
Cuts at: 2643 2643
Size: 4862
HaeII r_GCGC'y
Cuts at: 2296 2544 3566 4545 2296
Size: 248 1022 979 2613
Fragments arranged by size:
2613 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1011 1043 1291 1323 1329 1361 2704 2734 2736
Size: 32 248 32 6 32 1343 30 2
Cuts at: 2736 2766 1011
Size: 30 3107
Fragments arranged by size:
3107 1343 248 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 717 809 1901 717
Size: 92 1092 3678
Fragments arranged by size:
3678 1092 92
HindIII A'AGCT_T
Cuts at: 1451 1451
Size: 4862
HpaI GTT'AAC
Cuts at: 809 809
Size: 4862
KasI G'GCGC_C
Cuts at: 2292 2540 2292
Size: 248 4614
KpnI G_GTAC'C
Cuts at: 1882 1882
Size: 4862
MluI A'CGCG_T
Cuts at: 4295 4295
Size: 4862
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1040 1573 3533 3717 1040
Size: 533 1960 184 2185
Fragments arranged by size:
2185 1960 533 184
MscI TGG'CCA
Cuts at: 2623 2623
Size: 4862
MslI CAynn'nnrTG
Cuts at: 2851 2978 2851
Size: 127 4735
MspA1I CmG'CkG
Cuts at: 1973 2287 2647 3660 3905 1973
Size: 314 360 1013 245 2930
Fragments arranged by size:
2930 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3043 3043
Size: 4862
NarI GG'CG_CC
Cuts at: 2293 2541 2293
Size: 248 4614
NgoMIV G'CCGG_C
Cuts at: 3041 3041
Size: 4862
NheI G'CTAG_C
Cuts at: 1356 1356
Size: 4862
NspI r_CATG'y
Cuts at: 984 1915 2415 3322 984
Size: 931 500 907 2524
Fragments arranged by size:
2524 931 907 500
PciI A'CATG_T
Cuts at: 3318 3318
Size: 4862
PmeI GTTT'AAAC
Cuts at: 1861 1861
Size: 4862
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 4001
Size: 32 4830
PsiI TTA'TAA
Cuts at: 877 877
Size: 4862
PstI C_TGCA'G
Cuts at: 251 474 1909 2594 251
Size: 223 1435 685 2519
Fragments arranged by size:
2519 1435 685 223
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4862
PvuII CAG'CTG
Cuts at: 2287 2647 2287
Size: 360 4502
RsrII CG'GwC_CG
Cuts at: 3057 3057
Size: 4862
SacI G_AGCT'C
Cuts at: 1876 1876
Size: 4862
SalI G'TCGA_C
Cuts at: 1899 1899
Size: 4862
SapI GCTCTTCn'nnn_
Cuts at: 1136 1166 2885 3095 1136
Size: 30 1719 210 2903
Fragments arranged by size:
2903 1719 210 30
SbfI CC_TGCA'GG
Cuts at: 1909 1909
Size: 4862
ScaI AGT'ACT
Cuts at: 4379 4379
Size: 4862
SexAI A'CCwGG_T
Cuts at: 200 200
Size: 4862
SfcI C'TryA_G
Cuts at: 33 247 470 1905 2590 3583 3774 33
Size: 214 223 1435 685 993 191 1121
Fragments arranged by size:
1435 1121 993 685 223 214 191
SfoI GGC'GCC
Cuts at: 2294 2542 2294
Size: 248 4614
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4862
SmaI CCC'GGG
Cuts at: 1884 1884
Size: 4862
SmlI C'TyrA_G
Cuts at: 2017 2932 3424 3686 2017
Size: 915 492 262 3193
Fragments arranged by size:
3193 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4862
SphI G_CATG'C
Cuts at: 1915 2415 1915
Size: 500 4362
SspI AAT'ATT
Cuts at: 786 849 786
Size: 63 4799
StyI C'CwwG_G
Cuts at: 1996 2350 1996
Size: 354 4508
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2445 2578 3037 3246 2445
Size: 133 459 209 4061
Fragments arranged by size:
4061 459 209 133
TatI w'GTAC_w
Cuts at: 1011 1790 4377 1011
Size: 779 2587 1496
Fragments arranged by size:
2587 1496 779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4379 4411 4379
Size: 32 4830
Tth111I GACn'n_nGTC
Cuts at: 2659 2659
Size: 4862
XbaI T'CTAG_A
Cuts at: 699 1224 1398 1893 699
Size: 525 174 495 3668
Fragments arranged by size:
3668 525 495 174
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 505 2357 505
Size: 1852 3010
XmaI C'CCGG_G
Cuts at: 1882 1882
Size: 4862
XmnI GAAnn'nnTTC
Cuts at: 386 849 386
Size: 463 4399
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PmeI PpiI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI CspCI DraIII EcoNI EcoRV
FseI FspAI MfeI NcoI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI