Restriction map for
pF1KB6123
(Circular) (Six-base) MAPSORT of: pF1KB6123.seq Check: 8517 from: 1 to: 4871
pF1KB6123 4871 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 209 894 1909 209
Size: 685 1015 3171
Fragments arranged by size:
3171 1015 685
Acc65I G'GTAC_C
Cuts at: 1887 1887
Size: 4871
AclI AA'CG_TT
Cuts at: 4711 4711
Size: 4871
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 259 482 605 773 2696 3128 3875 4143
Size: 69 223 123 168 1923 432 747 268
Cuts at: 4143 190
Size: 918
Fragments arranged by size:
1923 918 747 432 268 223 168 123
69
AflIII A'CryG_T
Cuts at: 2223 3327 4304 2223
Size: 1104 977 2790
Fragments arranged by size:
2790 1104 977
AgeI A'CCGG_T
Cuts at: 3259 3259
Size: 4871
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1205 1209 1205
Size: 4 4867
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 211 243 211
Size: 32 4839
AlwNI CAG_nnn'CTG
Cuts at: 2176 3743 2176
Size: 1567 3304
ApaLI G'TGCA_C
Cuts at: 124 639 3641 124
Size: 515 3002 1354
Fragments arranged by size:
3002 1354 515
ApoI r'AATT_y
Cuts at: 488 804 831 988 1875 4224 4480 488
Size: 316 27 157 887 2349 256 879
Fragments arranged by size:
2349 887 879 316 256 157 27
AseI AT'TA_AT
Cuts at: 20 20
Size: 4871
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
AvaI C'yCGr_G
Cuts at: 1891 1891
Size: 4871
BamHI G'GATC_C
Cuts at: 1896 1896
Size: 4871
BanI G'GyrC_C
Cuts at: 187 1887 2301 2549 2584 187
Size: 1700 414 248 35 2474
Fragments arranged by size:
2474 1700 414 248 35
BanII G_rGCy'C
Cuts at: 146 419 1885 2915 146
Size: 273 1466 1030 2102
Fragments arranged by size:
2102 1466 1030 273
BbeI G_GCGC'C
Cuts at: 191 2305 2553 191
Size: 2114 248 2509
Fragments arranged by size:
2509 2114 248
BbsI GAAGACnn'nnnn_
Cuts at: 1594 1594
Size: 4871
BbvCI CC'TCA_GC
Cuts at: 1529 1529
Size: 4871
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 819 853 819
Size: 34 4837
BciVI GTATCCnnnnn_n'
Cuts at: 1477 2769 3530 4871 1477
Size: 1292 761 1341 1477
Fragments arranged by size:
1477 1341 1292 761
BclI T'GATC_A
Cuts at: 2391 2391
Size: 4871
BglI GCCn_nnn'nGGC
Cuts at: 1096 1112 1096
Size: 16 4855
BglII A'GATC_T
Cuts at: 918 1565 2386 4423 918
Size: 647 821 2037 1366
Fragments arranged by size:
2037 1366 821 647
BlpI GC'TnA_GC
Cuts at: 1983 1983
Size: 4871
Bme1580I G_kGCm'C
Cuts at: 128 643 1783 2496 2589 3645 128
Size: 515 1140 713 93 1056 1354
Fragments arranged by size:
1354 1140 1056 713 515 93
BmrI ACTGGGnnnn_n'
Cuts at: 2083 2164 2499 4643 2083
Size: 81 335 2144 2311
Fragments arranged by size:
2311 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3238 3238
Size: 4871
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 488
Size: 4871
Bpu10I CC'TnA_GC
Cuts at: 1529 1529
Size: 4871
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 1685 2047 2926 3418 3716 304
Size: 1381 362 879 492 298 1459
Fragments arranged by size:
1459 1381 879 492 362 298
BsaI GGTCTCn'nnnn_
Cuts at: 1792 4575 1792
Size: 2783 2088
BsaAI yAC'GTr
Cuts at: 2224 2224
Size: 4871
BsaBI GATnn'nnATC
Cuts at: 6 299 1073 2385 4422 6
Size: 293 774 1312 2037 455
Fragments arranged by size:
2037 1312 774 455 293
BsaHI Gr'CG_yC
Cuts at: 188 2302 2550 3252 4748 188
Size: 2114 248 702 1496 311
Fragments arranged by size:
2114 1496 702 311 248
BsaWI w'CCGG_w
Cuts at: 2060 2581 3259 3533 3680 4091 2060
Size: 521 678 274 147 411 2840
Fragments arranged by size:
2840 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 461 491 461
Size: 30 4841
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 228 638 228
Size: 410 4461
BseYI C'CCAG_C
Cuts at: 2296 3054 3631 4127 2296
Size: 758 577 496 3040
Fragments arranged by size:
3040 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2413 2459 3667 104
Size: 2309 46 1208 1308
Fragments arranged by size:
2309 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 128 643 1861 1885 2663 2853 3645 128
Size: 515 1218 24 778 190 792 1354
Fragments arranged by size:
1354 1218 792 778 515 190 24
BsmI GAATG_Cn'
Cuts at: 179 831 1458 179
Size: 652 627 3592
Fragments arranged by size:
3592 652 627
BsmBI CGTCTCn'nnnn_
Cuts at: 428 428
Size: 4871
Bsp1286I G_dGCh'C
Cuts at: 128 146 419 643 1783 1861 1885 2496 2589
Size: 18 273 224 1140 78 24 611 93
Cuts at: 2589 2663 2853 2915 3645 128
Size: 74 190 62 730 1354
Fragments arranged by size:
1354 1140 730 611 273 224 190 93
78 74 62 24 18
BspEI T'CCGG_A
Cuts at: 4091 4091
Size: 4871
BspHI T'CATG_A
Cuts at: 1136 4047 1136
Size: 2911 1960
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1921 2135 2437 2818 1921
Size: 214 302 381 3974
Fragments arranged by size:
3974 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3163 3217 4701 4866 50
Size: 3113 54 1484 165 55
Fragments arranged by size:
3113 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2783 2783
Size: 4871
BsrFI r'CCGG_y
Cuts at: 232 2869 3050 3259 232
Size: 2637 181 209 1844
Fragments arranged by size:
2637 1844 209 181
BssSI C'ACGA_G
Cuts at: 1205 3142 3500 1205
Size: 1937 358 2576
Fragments arranged by size:
2576 1937 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2248 2248
Size: 4871
BstBI TT'CG_AA
Cuts at: 3232 3232
Size: 4871
BstYI r'GATC_y
Cuts at: 1 288 918 1374 1565 1896 2363 2386 2721
Size: 287 630 456 191 331 467 23 335
Cuts at: 2721 2967 3979 4065 4077 4423 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 630 467 456 449 346 335 331
287 246 191 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1079 1079
Size: 4871
BtgI C'CryG_G
Cuts at: 107 1120 1775 107
Size: 1013 655 3203
Fragments arranged by size:
3203 1013 655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2821 3002 3165 2821
Size: 181 163 4527
Fragments arranged by size:
4527 181 163
BtsI GCAGTG_nn'
Cuts at: 737 1338 1692 2114 737
Size: 601 354 422 3494
Fragments arranged by size:
3494 601 422 354
DraI TTT'AAA
Cuts at: 1870 1870
Size: 4871
DrdI GACnn_nn'nnGTC
Cuts at: 2577 3435 2577
Size: 858 4013
EaeI y'GGCC_r
Cuts at: 736 2456 2630 3021 3048 4393 736
Size: 1720 174 391 27 1345 1214
Fragments arranged by size:
1720 1345 1214 391 174 27
EagI C'GGCC_G
Cuts at: 2456 2456
Size: 4871
EarI CTCTTCn'nnn_
Cuts at: 689 1811 2894 3104 689
Size: 1122 1083 210 2456
Fragments arranged by size:
2456 1122 1083 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 758 1114 3079 3389 3535 4681 758
Size: 356 1965 310 146 1146 948
Fragments arranged by size:
1965 1146 948 356 310 146
EcoICRI GAG'CTC
Cuts at: 1883 1883
Size: 4871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 259 482 488 605 773 2696 3128 3875
Size: 69 223 6 117 168 1923 432 747
Cuts at: 3875 4143 190
Size: 268 918
Fragments arranged by size:
1923 918 747 432 268 223 168 117
69 6
EcoO109I rG'GnC_Cy
Cuts at: 1212 2010 1212
Size: 798 4073
EcoRI G'AATT_C
Cuts at: 831 1875 831
Size: 1044 3827
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 809 2890 809
Size: 2081 2790
FspI TGC'GCA
Cuts at: 2652 2652
Size: 4871
HaeII r_GCGC'y
Cuts at: 191 2305 2553 3575 4554 191
Size: 2114 248 1022 979 508
Fragments arranged by size:
2114 1022 979 508 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 461 493 706 738 1155 1187 1359 1376 1391
Size: 32 213 32 417 32 172 17 15
Cuts at: 1391 1408 1821 1853 2713 2743 2745 2775 461
Size: 17 413 32 860 30 2 30 2557
Fragments arranged by size:
2557 860 417 413 213 172 32 32
32 32 30 30 17 17 15 2
HincII GTy'rAC
Cuts at: 895 1910 895
Size: 1015 3856
HindIII A'AGCT_T
Cuts at: 791 791
Size: 4871
KasI G'GCGC_C
Cuts at: 187 2301 2549 187
Size: 2114 248 2509
Fragments arranged by size:
2509 2114 248
KpnI G_GTAC'C
Cuts at: 1891 1891
Size: 4871
MfeI C'AATT_G
Cuts at: 398 398
Size: 4871
MluI A'CGCG_T
Cuts at: 4304 4304
Size: 4871
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1276 3542 3726 1141
Size: 135 2266 184 2286
Fragments arranged by size:
2286 2266 184 135
MscI TGG'CCA
Cuts at: 738 2632 738
Size: 1894 2977
MslI CAynn'nnrTG
Cuts at: 112 2860 2987 112
Size: 2748 127 1996
Fragments arranged by size:
2748 1996 127
MspA1I CmG'CkG
Cuts at: 203 1982 2296 2656 3669 3914 203
Size: 1779 314 360 1013 245 1160
Fragments arranged by size:
1779 1160 1013 360 314 245
NaeI GCC'GGC
Cuts at: 234 3052 234
Size: 2818 2053
NarI GG'CG_CC
Cuts at: 188 2302 2550 188
Size: 2114 248 2509
Fragments arranged by size:
2509 2114 248
NcoI C'CATG_G
Cuts at: 107 1120 1775 107
Size: 1013 655 3203
Fragments arranged by size:
3203 1013 655
NgoMIV G'CCGG_C
Cuts at: 232 3050 232
Size: 2818 2053
NspI r_CATG'y
Cuts at: 1186 1924 2424 3331 1186
Size: 738 500 907 2726
Fragments arranged by size:
2726 907 738 500
PciI A'CATG_T
Cuts at: 3327 3327
Size: 4871
PfoI T'CCnGG_A
Cuts at: 215 215
Size: 4871
PmeI GTTT'AAAC
Cuts at: 1870 1870
Size: 4871
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4010 4042 4010
Size: 32 4839
PpuMI rG'GwC_Cy
Cuts at: 1212 1212
Size: 4871
PsiI TTA'TAA
Cuts at: 557 557
Size: 4871
PstI C_TGCA'G
Cuts at: 732 1662 1918 2603 732
Size: 930 256 685 3000
Fragments arranged by size:
3000 930 685 256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4871
PvuII CAG'CTG
Cuts at: 2296 2656 2296
Size: 360 4511
RsrII CG'GwC_CG
Cuts at: 3066 3066
Size: 4871
SacI G_AGCT'C
Cuts at: 1885 1885
Size: 4871
SalI G'TCGA_C
Cuts at: 893 1908 893
Size: 1015 3856
SapI GCTCTTCn'nnn_
Cuts at: 689 2894 3104 689
Size: 2205 210 2456
Fragments arranged by size:
2456 2205 210
SbfI CC_TGCA'GG
Cuts at: 1918 1918
Size: 4871
ScaI AGT'ACT
Cuts at: 4388 4388
Size: 4871
SfcI C'TryA_G
Cuts at: 33 728 1658 1914 2599 3592 3783 33
Size: 695 930 256 685 993 191 1121
Fragments arranged by size:
1121 993 930 695 685 256 191
SfoI GGC'GCC
Cuts at: 189 2303 2551 189
Size: 2114 248 2509
Fragments arranged by size:
2509 2114 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4871
SmaI CCC'GGG
Cuts at: 1893 1893
Size: 4871
SmlI C'TyrA_G
Cuts at: 283 1700 2026 2941 3433 3695 283
Size: 1417 326 915 492 262 1459
Fragments arranged by size:
1459 1417 915 492 326 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4871
SphI G_CATG'C
Cuts at: 1186 1924 2424 1186
Size: 738 500 3633
Fragments arranged by size:
3633 738 500
StyI C'CwwG_G
Cuts at: 107 1063 1120 1775 2005 2359 107
Size: 956 57 655 230 354 2619
Fragments arranged by size:
2619 956 655 354 230 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 786 2454 2587 3046 3255 786
Size: 1668 133 459 209 2402
Fragments arranged by size:
2402 1668 459 209 133
TatI w'GTAC_w
Cuts at: 4386 4386
Size: 4871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4388 4420 4388
Size: 32 4839
Tth111I GACn'n_nGTC
Cuts at: 860 1598 2668 860
Size: 738 1070 3063
Fragments arranged by size:
3063 1070 738
XbaI T'CTAG_A
Cuts at: 1902 1902
Size: 4871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 4871
XmaI C'CCGG_G
Cuts at: 1891 1891
Size: 4871
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BfrBI BmgBI BmtI BsgI BsiWI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI