Restriction map for
pF1KB6131
(Circular) (Six-base) MAPSORT of: pF1KB6131.seq Check: 2689 from: 1 to: 4814
pF1KB6131 4814 bp
With 182 enzymes: SgfI *
June 2, 2009 16:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 663 987 1487 1511 663
Size: 324 500 24 3966
Fragments arranged by size:
3966 500 324 24
AccI GT'mk_AC
Cuts at: 1852 1852
Size: 4814
Acc65I G'GTAC_C
Cuts at: 1830 1830
Size: 4814
AclI AA'CG_TT
Cuts at: 4654 4654
Size: 4814
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 628 761 1705 2639 3071 3818 4086 628
Size: 133 944 934 432 747 268 1356
Fragments arranged by size:
1356 944 934 747 432 268 133
AflIII A'CryG_T
Cuts at: 882 1265 2166 3270 4247 882
Size: 383 901 1104 977 1449
Fragments arranged by size:
1449 1104 977 901 383
AgeI A'CCGG_T
Cuts at: 1436 3202 1436
Size: 1766 3048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 934 934
Size: 4814
AlwNI CAG_nnn'CTG
Cuts at: 291 318 576 1797 2119 3686 291
Size: 27 258 1221 322 1567 1419
Fragments arranged by size:
1567 1419 1221 322 258 27
ApaI G_GGCC'C
Cuts at: 214 214
Size: 4814
ApaLI G'TGCA_C
Cuts at: 3584 3584
Size: 4814
ApoI r'AATT_y
Cuts at: 1818 4167 4423 1818
Size: 2349 256 2209
Fragments arranged by size:
2349 2209 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4814
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
AvaI C'yCGr_G
Cuts at: 160 215 332 418 696 1834 160
Size: 55 117 86 278 1138 3140
Fragments arranged by size:
3140 1138 278 117 86 55
BamHI G'GATC_C
Cuts at: 1839 1839
Size: 4814
BanI G'GyrC_C
Cuts at: 999 1032 1403 1444 1664 1830 2244 2492 2527
Size: 33 371 41 220 166 414 248 35
Cuts at: 2527 999
Size: 3286
Fragments arranged by size:
3286 414 371 248 220 166 41 35
33
BanII G_rGCy'C
Cuts at: 118 214 419 521 584 1180 1828 2858 118
Size: 96 205 102 63 596 648 1030 2074
Fragments arranged by size:
2074 1030 648 596 205 102 96 63
BbeI G_GCGC'C
Cuts at: 2248 2496 2248
Size: 248 4566
BbsI GAAGACnn'nnnn_
Cuts at: 458 458
Size: 4814
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 823 857 823
Size: 34 4780
BciVI GTATCCnnnnn_n'
Cuts at: 1490 2712 3473 4814 1490
Size: 1222 761 1341 1490
Fragments arranged by size:
1490 1341 1222 761
BclI T'GATC_A
Cuts at: 2334 2334
Size: 4814
BglI GCCn_nnn'nGGC
Cuts at: 270 270
Size: 4814
BglII A'GATC_T
Cuts at: 852 2329 4366 852
Size: 1477 2037 1300
Fragments arranged by size:
2037 1477 1300
BlpI GC'TnA_GC
Cuts at: 607 1926 607
Size: 1319 3495
Bme1580I G_kGCm'C
Cuts at: 214 923 1406 2439 2532 3588 214
Size: 709 483 1033 93 1056 1440
Fragments arranged by size:
1440 1056 1033 709 483 93
BmrI ACTGGGnnnn_n'
Cuts at: 613 2026 2107 2442 4586 613
Size: 1413 81 335 2144 841
Fragments arranged by size:
2144 1413 841 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3181 3181
Size: 4814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 313 328 670 889 937 1104 1317 1504
Size: 170 15 342 219 48 167 213 187
Cuts at: 1504 143
Size: 3453
Fragments arranged by size:
3453 342 219 213 187 170 167 48
15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 1990 2869 3361 3659 424
Size: 1566 879 492 298 1579
Fragments arranged by size:
1579 1566 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 417 822 933 4518 417
Size: 405 111 3585 713
Fragments arranged by size:
3585 713 405 111
BsaAI yAC'GTr
Cuts at: 1266 2167 1266
Size: 901 3913
BsaBI GATnn'nnATC
Cuts at: 6 2328 4365 6
Size: 2322 2037 455
Fragments arranged by size:
2322 2037 455
BsaHI Gr'CG_yC
Cuts at: 174 2245 2493 3195 4691 174
Size: 2071 248 702 1496 297
Fragments arranged by size:
2071 1496 702 297 248
BsaWI w'CCGG_w
Cuts at: 1214 1436 2003 2524 3202 3476 3623 4034 1214
Size: 222 567 521 678 274 147 411 1994
Fragments arranged by size:
1994 678 567 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1123 1153 1226 1256 1468 1498 1123
Size: 30 73 30 212 30 4439
Fragments arranged by size:
4439 212 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 883 1003 1741 126
Size: 757 120 738 3199
Fragments arranged by size:
3199 757 738 120
BseYI C'CCAG_C
Cuts at: 154 347 551 845 1050 1142 1170 2239 2997
Size: 193 204 294 205 92 28 1069 758
Cuts at: 2997 3574 4070 154
Size: 577 496 898
Fragments arranged by size:
1069 898 758 577 496 294 205 204
193 92 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1143 1264 1753 1143
Size: 121 489 4204
Fragments arranged by size:
4204 489 121
BsiEI CG_ry'CG
Cuts at: 104 2356 2402 3610 104
Size: 2252 46 1208 1308
Fragments arranged by size:
2252 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1360 1828 2606 2796 3588 1360
Size: 468 778 190 792 2586
Fragments arranged by size:
2586 792 778 468 190
BsmBI CGTCTCn'nnnn_
Cuts at: 181 181
Size: 4814
Bsp1286I G_dGCh'C
Cuts at: 118 214 419 521 584 923 1180 1360 1406
Size: 96 205 102 63 339 257 180 46
Cuts at: 1406 1828 2439 2532 2606 2796 2858 3588 118
Size: 422 611 93 74 190 62 730 1344
Fragments arranged by size:
1344 730 611 422 339 257 205 190
180 102 96 93 74 63 62 46
BspEI T'CCGG_A
Cuts at: 4034 4034
Size: 4814
BspHI T'CATG_A
Cuts at: 3990 3990
Size: 4814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 663 987 1487 1511 1864 2078 2380 2761 663
Size: 324 500 24 353 214 302 381 2716
Fragments arranged by size:
2716 500 381 353 324 302 214 24
BsrBI CCG'CTC
Cuts at: 50 374 3106 3160 4644 4809 50
Size: 324 2732 54 1484 165 55
Fragments arranged by size:
2732 1484 324 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2726 2726
Size: 4814
BsrFI r'CCGG_y
Cuts at: 510 1436 1490 1524 1712 2812 2993 3202 510
Size: 926 54 34 188 1100 181 209 2122
Fragments arranged by size:
2122 1100 926 209 188 181 54 34
BssSI C'ACGA_G
Cuts at: 1351 1417 1759 3085 3443 1351
Size: 66 342 1326 358 2722
Fragments arranged by size:
2722 1326 358 342 66
BstAPI GCAn_nnn'nTGC
Cuts at: 2191 2191
Size: 4814
BstBI TT'CG_AA
Cuts at: 3175 3175
Size: 4814
BstEII G'GTnAC_C
Cuts at: 675 675
Size: 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 564 564
Size: 4814
BstYI r'GATC_y
Cuts at: 1 338 762 852 900 1839 2306 2329 2664
Size: 337 424 90 48 939 467 23 335
Cuts at: 2664 2910 3922 4008 4020 4366 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 939 467 449 424 346 337 335
246 90 86 48 23 12
BtgI C'CryG_G
Cuts at: 392 392
Size: 4814
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2764 2945 3108 2764
Size: 181 163 4470
Fragments arranged by size:
4470 181 163
BtsI GCAGTG_nn'
Cuts at: 439 2057 439
Size: 1618 3196
DraI TTT'AAA
Cuts at: 1813 1813
Size: 4814
DrdI GACnn_nn'nnGTC
Cuts at: 2520 3378 2520
Size: 858 3956
EaeI y'GGCC_r
Cuts at: 320 389 2399 2573 2964 2991 4336 320
Size: 69 2010 174 391 27 1345 798
Fragments arranged by size:
2010 1345 798 391 174 69 27
EagI C'GGCC_G
Cuts at: 2399 2399
Size: 4814
EarI CTCTTCn'nnn_
Cuts at: 184 644 737 1160 1450 1528 2837 3047 184
Size: 460 93 423 290 78 1309 210 1951
Fragments arranged by size:
1951 1309 460 423 290 210 93 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 1453 1509 1744 3022 3332 3478 4624 172
Size: 1281 56 235 1278 310 146 1146 362
Fragments arranged by size:
1281 1278 1146 362 310 235 146 56
EcoICRI GAG'CTC
Cuts at: 1826 1826
Size: 4814
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 143 313 328 628 670 761 889 937 1104
Size: 170 15 300 42 91 128 48 167
Cuts at: 1104 1317 1504 1705 2639 3071 3818 4086 143
Size: 213 187 201 934 432 747 268 871
Fragments arranged by size:
934 871 747 432 300 268 213 201
187 170 167 128 91 48 42 15
EcoNI CCTnn'n_nnAGG
Cuts at: 643 643
Size: 4814
EcoO109I rG'GnC_Cy
Cuts at: 211 222 240 615 1392 1570 1953 211
Size: 11 18 375 777 178 383 3072
Fragments arranged by size:
3072 777 383 375 178 18 11
EcoRI G'AATT_C
Cuts at: 1818 1818
Size: 4814
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2833 2833
Size: 4814
FspI TGC'GCA
Cuts at: 2595 2595
Size: 4814
HaeII r_GCGC'y
Cuts at: 234 1767 1782 2248 2496 3518 4497 234
Size: 1533 15 466 248 1022 979 551
Fragments arranged by size:
1533 1022 979 551 466 248 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 736 768 779 811 1123 1155 2656 2686 2688
Size: 32 11 32 312 32 1501 30 2
Cuts at: 2688 2718 736
Size: 30 2832
Fragments arranged by size:
2832 1501 312 32 32 32 30 30
11 2
HincII GTy'rAC
Cuts at: 1305 1853 1305
Size: 548 4266
KasI G'GCGC_C
Cuts at: 2244 2492 2244
Size: 248 4566
KpnI G_GTAC'C
Cuts at: 1834 1834
Size: 4814
MluI A'CGCG_T
Cuts at: 882 4247 882
Size: 3365 1449
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3485 3669 3485
Size: 184 4630
MscI TGG'CCA
Cuts at: 322 2575 322
Size: 2253 2561
MslI CAynn'nnrTG
Cuts at: 2803 2930 2803
Size: 127 4687
MspA1I CmG'CkG
Cuts at: 394 507 611 1054 1295 1584 1644 1925 2239
Size: 113 104 443 241 289 60 281 314
Cuts at: 2239 2599 3612 3857 394
Size: 360 1013 245 1351
Fragments arranged by size:
1351 1013 443 360 314 289 281 245
241 113 104 60
NaeI GCC'GGC
Cuts at: 2995 2995
Size: 4814
NarI GG'CG_CC
Cuts at: 2245 2493 2245
Size: 248 4566
NgoMIV G'CCGG_C
Cuts at: 2993 2993
Size: 4814
NspI r_CATG'y
Cuts at: 1867 2367 3274 1867
Size: 500 907 3407
Fragments arranged by size:
3407 907 500
PasI CC'CwG_GG
Cuts at: 141 861 141
Size: 720 4094
PciI A'CATG_T
Cuts at: 3270 3270
Size: 4814
PflMI CCAn_nnn'nTGG
Cuts at: 1527 1527
Size: 4814
PmeI GTTT'AAAC
Cuts at: 1813 1813
Size: 4814
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 3953
Size: 32 4782
PpuMI rG'GwC_Cy
Cuts at: 240 615 1392 1570 240
Size: 375 777 178 3484
Fragments arranged by size:
3484 777 375 178
PshAI GACnn'nnGTC
Cuts at: 1568 1568
Size: 4814
PspOMI G'GGCC_C
Cuts at: 210 210
Size: 4814
PstI C_TGCA'G
Cuts at: 230 998 1508 1861 2546 230
Size: 768 510 353 685 2498
Fragments arranged by size:
2498 768 685 510 353
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4814
PvuII CAG'CTG
Cuts at: 507 1054 2239 2599 507
Size: 547 1185 360 2722
Fragments arranged by size:
2722 1185 547 360
RsrII CG'GwC_CG
Cuts at: 3009 3009
Size: 4814
SacI G_AGCT'C
Cuts at: 1828 1828
Size: 4814
SacII CC_GC'GG
Cuts at: 395 395
Size: 4814
SalI G'TCGA_C
Cuts at: 1851 1851
Size: 4814
SanDI GG'GwC_CC
Cuts at: 615 1570 615
Size: 955 3859
SapI GCTCTTCn'nnn_
Cuts at: 1160 1450 2837 3047 1160
Size: 290 1387 210 2927
Fragments arranged by size:
2927 1387 290 210
SbfI CC_TGCA'GG
Cuts at: 1861 1861
Size: 4814
ScaI AGT'ACT
Cuts at: 4331 4331
Size: 4814
SfcI C'TryA_G
Cuts at: 33 226 994 1504 1857 2542 3535 3726 33
Size: 193 768 510 353 685 993 191 1121
Fragments arranged by size:
1121 993 768 685 510 353 193 191
SfoI GGC'GCC
Cuts at: 2246 2494 2246
Size: 248 4566
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4814
SgrAI Cr'CCGG_yG
Cuts at: 1490 1490
Size: 4814
SmaI CCC'GGG
Cuts at: 217 334 420 1836 217
Size: 117 86 1416 3195
Fragments arranged by size:
3195 1416 117 86
SmlI C'TyrA_G
Cuts at: 439 1969 2884 3376 3638 439
Size: 1530 915 492 262 1615
Fragments arranged by size:
1615 1530 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4814
SphI G_CATG'C
Cuts at: 1867 2367 1867
Size: 500 4314
StuI AGG'CCT
Cuts at: 1015 1636 1015
Size: 621 4193
StyI C'CwwG_G
Cuts at: 278 1948 2302 278
Size: 1670 354 2790
Fragments arranged by size:
2790 1670 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 339 2397 2530 2989 3198 339
Size: 2058 133 459 209 1955
Fragments arranged by size:
2058 1955 459 209 133
TatI w'GTAC_w
Cuts at: 4329 4329
Size: 4814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 1468 1500 4331 4363 1226
Size: 32 210 32 2831 32 1677
Fragments arranged by size:
2831 1677 210 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2611 2611
Size: 4814
XbaI T'CTAG_A
Cuts at: 759 1074 1845 759
Size: 315 771 3728
Fragments arranged by size:
3728 771 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1581 2309 1581
Size: 728 4086
XmaI C'CCGG_G
Cuts at: 215 332 418 1834 215
Size: 117 86 1416 3195
Fragments arranged by size:
3195 1416 117 86
XmnI GAAnn'nnTTC
Cuts at: 1288 1459 1636 1288
Size: 171 177 4466
Fragments arranged by size:
4466 177 171
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PsiI PspXI
PsrI SexAI SfiI SnaBI SrfI SspI SwaI XhoI
ZraI