Restriction map for
pF1KB6132
(Circular) (Six-base) MAPSORT of: pF1KB6132.seq Check: 1981 from: 1 to: 4997
pF1KB6132 4997 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 628 628
Size: 4997
AccI GT'mk_AC
Cuts at: 1009 2035 1009
Size: 1026 3971
Acc65I G'GTAC_C
Cuts at: 2013 2013
Size: 4997
AclI AA'CG_TT
Cuts at: 4837 4837
Size: 4997
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 2822 3254 4001 4269 1009
Size: 1813 432 747 268 1737
Fragments arranged by size:
1813 1737 747 432 268
AflIII A'CryG_T
Cuts at: 476 533 2349 3453 4430 476
Size: 57 1816 1104 977 1043
Fragments arranged by size:
1816 1104 1043 977 57
AgeI A'CCGG_T
Cuts at: 3385 3385
Size: 4997
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 206 206
Size: 4997
AlwNI CAG_nnn'CTG
Cuts at: 508 681 2302 3869 508
Size: 173 1621 1567 1636
Fragments arranged by size:
1636 1621 1567 173
ApaI G_GGCC'C
Cuts at: 147 1837 147
Size: 1690 3307
ApaLI G'TGCA_C
Cuts at: 1264 3767 1264
Size: 2503 2494
ApoI r'AATT_y
Cuts at: 2001 4350 4606 2001
Size: 2349 256 2392
Fragments arranged by size:
2392 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4997
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
AvaI C'yCGr_G
Cuts at: 321 392 744 838 1060 1091 1899 2017 321
Size: 71 352 94 222 31 808 118 3301
Fragments arranged by size:
3301 808 352 222 118 94 71 31
BamHI G'GATC_C
Cuts at: 2022 2022
Size: 4997
BanI G'GyrC_C
Cuts at: 431 687 1366 1720 1942 2013 2427 2675 2710
Size: 256 679 354 222 71 414 248 35
Cuts at: 2710 431
Size: 2718
Fragments arranged by size:
2718 679 414 354 256 248 222 71
35
BanII G_rGCy'C
Cuts at: 116 147 330 364 371 1205 1478 1732 1837
Size: 31 183 34 7 834 273 254 105
Cuts at: 1837 1869 2011 3041 116
Size: 32 142 1030 2072
Fragments arranged by size:
2072 1030 834 273 254 183 142 105
34 32 31 7
BbeI G_GCGC'C
Cuts at: 435 2431 2679 435
Size: 1996 248 2753
Fragments arranged by size:
2753 1996 248
BbsI GAAGACnn'nnnn_
Cuts at: 1701 1800 1701
Size: 99 4898
BbvCI CC'TCA_GC
Cuts at: 446 512 1408 446
Size: 66 896 4035
Fragments arranged by size:
4035 896 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1230 1264 1230
Size: 34 4963
BciVI GTATCCnnnnn_n'
Cuts at: 2895 3656 4997 2895
Size: 761 1341 2895
Fragments arranged by size:
2895 1341 761
BclI T'GATC_A
Cuts at: 2517 2517
Size: 4997
BglI GCCn_nnn'nGGC
Cuts at: 851 1661 1762 851
Size: 810 101 4086
Fragments arranged by size:
4086 810 101
BglII A'GATC_T
Cuts at: 2512 4549 2512
Size: 2037 2960
BlpI GC'TnA_GC
Cuts at: 1204 1891 2109 1204
Size: 687 218 4092
Fragments arranged by size:
4092 687 218
Bme1580I G_kGCm'C
Cuts at: 147 692 1268 1837 2622 2715 3771 147
Size: 545 576 569 785 93 1056 1373
Fragments arranged by size:
1373 1056 785 576 569 545 93
BmgBI CAC'GTC
Cuts at: 1149 1149
Size: 4997
BmrI ACTGGGnnnn_n'
Cuts at: 2209 2290 2625 4769 2209
Size: 81 335 2144 2437
Fragments arranged by size:
2437 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1640 3364 1640
Size: 1724 3273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 703 1099 1189 1219 1515 1581 1769 355
Size: 348 396 90 30 296 66 188 3583
Fragments arranged by size:
3583 396 348 296 188 90 66 30
Bpu10I CC'TnA_GC
Cuts at: 446 512 1408 1651 446
Size: 66 896 243 3792
Fragments arranged by size:
3792 896 243 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2173 3052 3544 3842 2173
Size: 879 492 298 3328
Fragments arranged by size:
3328 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 567 4701 567
Size: 4134 863
BsaAI yAC'GTr
Cuts at: 477 659 906 1374 2350 477
Size: 182 247 468 976 3124
Fragments arranged by size:
3124 976 468 247 182
BsaBI GATnn'nnATC
Cuts at: 6 2511 4548 6
Size: 2505 2037 455
Fragments arranged by size:
2505 2037 455
BsaHI Gr'CG_yC
Cuts at: 432 803 2428 2676 3378 4874 432
Size: 371 1625 248 702 1496 555
Fragments arranged by size:
1625 1496 702 555 371 248
BsaWI w'CCGG_w
Cuts at: 1948 2186 2707 3385 3659 3806 4217 1948
Size: 238 521 678 274 147 411 2728
Fragments arranged by size:
2728 678 521 411 274 238 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1496 1526 1742 1772 1496
Size: 30 216 30 4721
Fragments arranged by size:
4721 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1783 1967 1783
Size: 184 4813
BseYI C'CCAG_C
Cuts at: 936 944 997 2422 3180 3757 4253 936
Size: 8 53 1425 758 577 496 1680
Fragments arranged by size:
1680 1425 758 577 496 53 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 249 871 928 1171 1265 249
Size: 622 57 243 94 3981
Fragments arranged by size:
3981 622 243 94 57
BsiEI CG_ry'CG
Cuts at: 104 318 855 2539 2585 3793 104
Size: 214 537 1684 46 1208 1308
Fragments arranged by size:
1684 1308 1208 537 214 46
BsiHKAI G_wGCw'C
Cuts at: 330 371 905 1205 1268 2011 2789 2979 3771
Size: 41 534 300 63 743 778 190 792
Cuts at: 3771 330
Size: 1556
Fragments arranged by size:
1556 792 778 743 534 300 190 63
41
BsmBI CGTCTCn'nnnn_
Cuts at: 794 1499 1815 1976 794
Size: 705 316 161 3815
Fragments arranged by size:
3815 705 316 161
Bsp1286I G_dGCh'C
Cuts at: 116 147 330 364 371 692 905 1205 1268
Size: 31 183 34 7 321 213 300 63
Cuts at: 1268 1478 1732 1837 1869 2011 2622 2715 2789
Size: 210 254 105 32 142 611 93 74
Cuts at: 2789 2979 3041 3771 116
Size: 190 62 730 1342
Fragments arranged by size:
1342 730 611 321 300 254 213 210
190 183 142 105 93 74 63 62
34 32 31 7
BspEI T'CCGG_A
Cuts at: 1948 4217 1948
Size: 2269 2728
BspHI T'CATG_A
Cuts at: 4173 4173
Size: 4997
BspMI ACCTGCnnnn'nnnn_
Cuts at: 628 785 2047 2261 2563 2944 628
Size: 157 1262 214 302 381 2681
Fragments arranged by size:
2681 1262 381 302 214 157
BsrBI CCG'CTC
Cuts at: 50 320 1103 3289 3343 4827 4992 50
Size: 270 783 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 783 270 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2909 2909
Size: 4997
BsrFI r'CCGG_y
Cuts at: 126 1924 2995 3176 3385 126
Size: 1798 1071 181 209 1738
Fragments arranged by size:
1798 1738 1071 209 181
BssHII G'CGCG_C
Cuts at: 136 136
Size: 4997
BssSI C'ACGA_G
Cuts at: 3268 3626 3268
Size: 358 4639
BstAPI GCAn_nnn'nTGC
Cuts at: 508 1272 2374 508
Size: 764 1102 3131
Fragments arranged by size:
3131 1102 764
BstBI TT'CG_AA
Cuts at: 3358 3358
Size: 4997
BstXI CCAn_nnnn'nTGG
Cuts at: 944 1167 1314 944
Size: 223 147 4627
Fragments arranged by size:
4627 223 147
BstYI r'GATC_y
Cuts at: 1 2022 2489 2512 2847 3093 4105 4191 4203
Size: 2021 467 23 335 246 1012 86 12
Cuts at: 4203 4549 1
Size: 346 449
Fragments arranged by size:
2021 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 1010 1010
Size: 4997
BtgI C'CryG_G
Cuts at: 107 148 849 896 1533 1689 1766 107
Size: 41 701 47 637 156 77 3338
Fragments arranged by size:
3338 701 637 156 77 47 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1334 1673 2947 3128 3291 1334
Size: 339 1274 181 163 3040
Fragments arranged by size:
3040 1274 339 181 163
BtsI GCAGTG_nn'
Cuts at: 506 518 813 1248 2240 506
Size: 12 295 435 992 3263
Fragments arranged by size:
3263 992 435 295 12
DraI TTT'AAA
Cuts at: 1996 1996
Size: 4997
DrdI GACnn_nn'nnGTC
Cuts at: 2703 3561 2703
Size: 858 4139
EaeI y'GGCC_r
Cuts at: 852 1536 1754 1763 1769 2582 2756 3147 3174
Size: 684 218 9 6 813 174 391 27
Cuts at: 3174 4519 852
Size: 1345 1330
Fragments arranged by size:
1345 1330 813 684 391 218 174 27
9 6
EagI C'GGCC_G
Cuts at: 852 2582 852
Size: 1730 3267
EarI CTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 214 1009 1859 3205 3515 3661 4807 214
Size: 795 850 1346 310 146 1146 404
Fragments arranged by size:
1346 1146 850 795 404 310 146
EcoICRI GAG'CTC
Cuts at: 328 369 1203 2009 328
Size: 41 834 806 3316
Fragments arranged by size:
3316 834 806 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 355 703 1009 1099 1189 1219 1515 1581 1769
Size: 348 306 90 90 30 296 66 188
Cuts at: 1769 2822 3254 4001 4269 355
Size: 1053 432 747 268 1083
Fragments arranged by size:
1083 1053 747 432 348 306 296 268
188 90 90 66 30
EcoNI CCTnn'n_nnAGG
Cuts at: 838 838
Size: 4997
EcoO109I rG'GnC_Cy
Cuts at: 143 144 1106 1833 1834 2136 143
Size: 1 962 727 1 302 3004
Fragments arranged by size:
3004 962 727 302 1 1
EcoRI G'AATT_C
Cuts at: 2001 2001
Size: 4997
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4997
FspI TGC'GCA
Cuts at: 2778 2778
Size: 4997
HaeII r_GCGC'y
Cuts at: 435 681 1649 1704 2431 2679 3701 4680 435
Size: 246 968 55 727 248 1022 979 752
Fragments arranged by size:
1022 979 968 752 727 248 246 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1496 1528 1651 1683 1742 1774 2839 2869 2871
Size: 32 123 32 59 32 1065 30 2
Cuts at: 2871 2901 1496
Size: 30 3592
Fragments arranged by size:
3592 1065 123 59 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2036 2036
Size: 4997
KasI G'GCGC_C
Cuts at: 431 2427 2675 431
Size: 1996 248 2753
Fragments arranged by size:
2753 1996 248
KpnI G_GTAC'C
Cuts at: 2017 2017
Size: 4997
MfeI C'AATT_G
Cuts at: 1567 1567
Size: 4997
MluI A'CGCG_T
Cuts at: 4430 4430
Size: 4997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1706 1886 3668 3852 1706
Size: 180 1782 184 2851
Fragments arranged by size:
2851 1782 184 180
MscI TGG'CCA
Cuts at: 2758 2758
Size: 4997
MslI CAynn'nnrTG
Cuts at: 188 1765 2986 3113 188
Size: 1577 1221 127 2072
Fragments arranged by size:
2072 1577 1221 127
MspA1I CmG'CkG
Cuts at: 150 225 417 972 1465 1493 1535 1559 1851
Size: 75 192 555 493 28 42 24 292
Cuts at: 1851 1970 2108 2422 2782 3795 4040 150
Size: 119 138 314 360 1013 245 1107
Fragments arranged by size:
1107 1013 555 493 360 314 292 245
192 138 119 75 42 28 24
NaeI GCC'GGC
Cuts at: 3178 3178
Size: 4997
NarI GG'CG_CC
Cuts at: 432 2428 2676 432
Size: 1996 248 2753
Fragments arranged by size:
2753 1996 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4997
NgoMIV G'CCGG_C
Cuts at: 3176 3176
Size: 4997
NspI r_CATG'y
Cuts at: 2050 2550 3457 2050
Size: 500 907 3590
Fragments arranged by size:
3590 907 500
PasI CC'CwG_GG
Cuts at: 426 1314 426
Size: 888 4109
PciI A'CATG_T
Cuts at: 3453 3453
Size: 4997
PfoI T'CCnGG_A
Cuts at: 734 734
Size: 4997
PmeI GTTT'AAAC
Cuts at: 1996 1996
Size: 4997
PmlI CAC'GTG
Cuts at: 659 906 659
Size: 247 4750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4136 4168 4136
Size: 32 4965
PpuMI rG'GwC_Cy
Cuts at: 1106 1106
Size: 4997
PspOMI G'GGCC_C
Cuts at: 143 1833 143
Size: 1690 3307
PstI C_TGCA'G
Cuts at: 1120 2044 2729 1120
Size: 924 685 3388
Fragments arranged by size:
3388 924 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4997
PvuII CAG'CTG
Cuts at: 417 972 1493 1559 2422 2782 417
Size: 555 521 66 863 360 2632
Fragments arranged by size:
2632 863 555 521 360 66
RsrII CG'GwC_CG
Cuts at: 732 3192 732
Size: 2460 2537
SacI G_AGCT'C
Cuts at: 330 371 1205 2011 330
Size: 41 834 806 3316
Fragments arranged by size:
3316 834 806 41
SacII CC_GC'GG
Cuts at: 151 1536 151
Size: 1385 3612
SalI G'TCGA_C
Cuts at: 2034 2034
Size: 4997
SapI GCTCTTCn'nnn_
Cuts at: 3020 3230 3020
Size: 210 4787
SbfI CC_TGCA'GG
Cuts at: 2044 2044
Size: 4997
ScaI AGT'ACT
Cuts at: 4514 4514
Size: 4997
SfcI C'TryA_G
Cuts at: 33 828 1116 2040 2725 3718 3909 33
Size: 795 288 924 685 993 191 1121
Fragments arranged by size:
1121 993 924 795 685 288 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 851 1762 851
Size: 911 4086
SfoI GGC'GCC
Cuts at: 433 2429 2677 433
Size: 1996 248 2753
Fragments arranged by size:
2753 1996 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4997
SmaI CCC'GGG
Cuts at: 394 1093 2019 394
Size: 699 926 3372
Fragments arranged by size:
3372 926 699
SmlI C'TyrA_G
Cuts at: 1060 2152 3067 3559 3821 1060
Size: 1092 915 492 262 2236
Fragments arranged by size:
2236 1092 915 492 262
SnaBI TAC'GTA
Cuts at: 1374 1374
Size: 4997
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4997
SphI G_CATG'C
Cuts at: 2050 2550 2050
Size: 500 4497
StuI AGG'CCT
Cuts at: 742 1384 742
Size: 642 4355
StyI C'CwwG_G
Cuts at: 107 1298 1440 1478 2131 2485 107
Size: 1191 142 38 653 354 2619
Fragments arranged by size:
2619 1191 653 354 142 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 200 812 906 1432 1602 2580 2713 3172 3381
Size: 612 94 526 170 978 133 459 209
Cuts at: 3381 200
Size: 1816
Fragments arranged by size:
1816 978 612 526 459 209 170 133
94
TatI w'GTAC_w
Cuts at: 4512 4512
Size: 4997
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4514 4546 4514
Size: 32 4965
Tth111I GACn'n_nGTC
Cuts at: 582 964 1510 2794 582
Size: 382 546 1284 2785
Fragments arranged by size:
2785 1284 546 382
XbaI T'CTAG_A
Cuts at: 2028 2028
Size: 4997
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 2492
Size: 4997
XhoI C'TCGA_G
Cuts at: 1060 1060
Size: 4997
XmaI C'CCGG_G
Cuts at: 392 1091 2017 392
Size: 699 926 3372
Fragments arranged by size:
3372 926 699
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsmI BsrGI BstEII Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI NsiI PacI PflMI PshAI
PsiI PspXI PsrI SanDI SexAI SgrAI SrfI SspI
SwaI XmnI ZraI