Restriction map for
pF1KB6134
(Circular) (Six-base) MAPSORT of: pF1KB6134.seq Check: 6255 from: 1 to: 5006
pF1KB6134 5006 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 1066 1066
Size: 5006
AccI GT'mk_AC
Cuts at: 734 2044 734
Size: 1310 3696
Acc65I G'GTAC_C
Cuts at: 2022 2022
Size: 5006
AclI AA'CG_TT
Cuts at: 4846 4846
Size: 5006
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 1102 1375 1537 1555 2831 3263 4010 4278
Size: 321 273 162 18 1276 432 747 268
Cuts at: 4278 781
Size: 1509
Fragments arranged by size:
1509 1276 747 432 321 273 268 162
18
AfeI AGC'GCT
Cuts at: 940 1552 940
Size: 612 4394
AflIII A'CryG_T
Cuts at: 2358 3462 4439 2358
Size: 1104 977 2925
Fragments arranged by size:
2925 1104 977
AgeI A'CCGG_T
Cuts at: 3394 3394
Size: 5006
AleI CACnn'nnGTG
Cuts at: 1723 1723
Size: 5006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 142 921 142
Size: 779 4227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 803 835 803
Size: 32 4974
AlwNI CAG_nnn'CTG
Cuts at: 650 660 1164 1197 1641 2311 3878 650
Size: 10 504 33 444 670 1567 1778
Fragments arranged by size:
1778 1567 670 504 444 33 10
ApaLI G'TGCA_C
Cuts at: 3776 3776
Size: 5006
ApoI r'AATT_y
Cuts at: 2010 4359 4615 2010
Size: 2349 256 2401
Fragments arranged by size:
2401 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5006
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
AvaI C'yCGr_G
Cuts at: 1294 1325 2026 1294
Size: 31 701 4274
Fragments arranged by size:
4274 701 31
BamHI G'GATC_C
Cuts at: 1873 1983 2031 1873
Size: 110 48 4848
Fragments arranged by size:
4848 110 48
BanI G'GyrC_C
Cuts at: 473 788 1121 1259 2022 2436 2684 2719 473
Size: 315 333 138 763 414 248 35 2760
Fragments arranged by size:
2760 763 414 333 315 248 138 35
BanII G_rGCy'C
Cuts at: 644 668 746 2020 3050 644
Size: 24 78 1274 1030 2600
Fragments arranged by size:
2600 1274 1030 78 24
BbeI G_GCGC'C
Cuts at: 1125 1263 2440 2688 1125
Size: 138 1177 248 3443
Fragments arranged by size:
3443 1177 248 138
BbsI GAAGACnn'nnnn_
Cuts at: 964 1066 964
Size: 102 4904
BbvCI CC'TCA_GC
Cuts at: 712 712
Size: 5006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1026 1060 1026
Size: 34 4972
BciVI GTATCCnnnnn_n'
Cuts at: 301 2904 3665 5006 301
Size: 2603 761 1341 301
Fragments arranged by size:
2603 1341 761 301
BclI T'GATC_A
Cuts at: 512 2526 512
Size: 2014 2992
BfrBI ATG'CAT
Cuts at: 1761 1761
Size: 5006
BglI GCCn_nnn'nGGC
Cuts at: 613 622 692 613
Size: 9 70 4927
Fragments arranged by size:
4927 70 9
BglII A'GATC_T
Cuts at: 2521 4558 2521
Size: 2037 2969
BlpI GC'TnA_GC
Cuts at: 643 2118 643
Size: 1475 3531
Bme1580I G_kGCm'C
Cuts at: 791 2631 2724 3780 791
Size: 1840 93 1056 2017
Fragments arranged by size:
2017 1840 1056 93
BmgBI CAC'GTC
Cuts at: 165 165
Size: 5006
BmrI ACTGGGnnnn_n'
Cuts at: 813 2218 2299 2634 4778 813
Size: 1405 81 335 2144 1041
Fragments arranged by size:
2144 1405 1041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 613 682 1219 1420 1663 1796 280
Size: 333 69 537 201 243 133 3490
Fragments arranged by size:
3490 537 333 243 201 133 69
Bpu10I CC'TnA_GC
Cuts at: 712 712
Size: 5006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 184 2182 3061 3553 3851 115
Size: 69 1998 879 492 298 1270
Fragments arranged by size:
1998 1270 879 492 298 69
BsaI GGTCTCn'nnnn_
Cuts at: 4710 4710
Size: 5006
BsaAI yAC'GTr
Cuts at: 974 2359 974
Size: 1385 3621
BsaBI GATnn'nnATC
Cuts at: 6 1036 2520 4557 6
Size: 1030 1484 2037 455
Fragments arranged by size:
2037 1484 1030 455
BsaHI Gr'CG_yC
Cuts at: 1063 1122 1260 2437 2685 3387 4883 1063
Size: 59 138 1177 248 702 1496 1186
Fragments arranged by size:
1496 1186 1177 702 248 138 59
BsaWI w'CCGG_w
Cuts at: 1788 2195 2716 3394 3668 3815 4226 1788
Size: 407 521 678 274 147 411 2568
Fragments arranged by size:
2568 678 521 411 407 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1828 1858 1828
Size: 30 4976
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 364 610 1156 1186 1261 1264 1375 1378 1417
Size: 246 546 30 75 3 111 3 39
Cuts at: 1417 1516 1555 1660 1836 364
Size: 99 39 105 176 3534
Fragments arranged by size:
3534 546 246 176 111 105 99 75
39 39 30 3 3
BseYI C'CCAG_C
Cuts at: 194 832 1631 1868 2431 3189 3766 4262 194
Size: 638 799 237 563 758 577 496 938
Fragments arranged by size:
938 799 758 638 577 563 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 217
Size: 5006
BsiEI CG_ry'CG
Cuts at: 104 2548 2594 3802 104
Size: 2444 46 1208 1308
Fragments arranged by size:
2444 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 374 668 746 2020 2798 2988 3780 374
Size: 294 78 1274 778 190 792 1600
Fragments arranged by size:
1600 1274 792 778 294 190 78
Bsp1286I G_dGCh'C
Cuts at: 374 644 668 746 791 2020 2631 2724 2798
Size: 270 24 78 45 1229 611 93 74
Cuts at: 2798 2988 3050 3780 374
Size: 190 62 730 1600
Fragments arranged by size:
1600 1229 730 611 270 190 93 78
74 62 45 24
BspEI T'CCGG_A
Cuts at: 4226 4226
Size: 5006
BspHI T'CATG_A
Cuts at: 4182 4182
Size: 5006
BspMI ACCTGCnnnn'nnnn_
Cuts at: 548 2056 2270 2572 2953 548
Size: 1508 214 302 381 2601
Fragments arranged by size:
2601 1508 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1119 1182 1233 1616 3298 3352 4836 5001
Size: 1069 63 51 383 1682 54 1484 165
Cuts at: 5001 50
Size: 55
Fragments arranged by size:
1682 1484 1069 383 165 63 55 54
51
BsrDI GCAATG_nn'
Cuts at: 1914 2918 1914
Size: 1004 4002
BsrFI r'CCGG_y
Cuts at: 1661 1751 3004 3185 3394 1661
Size: 90 1253 181 209 3273
Fragments arranged by size:
3273 1253 209 181 90
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 5006
BssSI C'ACGA_G
Cuts at: 727 3277 3635 727
Size: 2550 358 2098
Fragments arranged by size:
2550 2098 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1164 1419 1527 2383 1164
Size: 255 108 856 3787
Fragments arranged by size:
3787 856 255 108
BstBI TT'CG_AA
Cuts at: 3367 3367
Size: 5006
BstEII G'GTnAC_C
Cuts at: 1791 1791
Size: 5006
BstXI CCAn_nnnn'nTGG
Cuts at: 1032 1494 1032
Size: 462 4544
BstYI r'GATC_y
Cuts at: 1 1873 1983 2031 2498 2521 2856 3102 4114
Size: 1872 110 48 467 23 335 246 1012
Cuts at: 4114 4200 4212 4558 1
Size: 86 12 346 449
Fragments arranged by size:
1872 1012 467 449 346 335 246 110
86 48 23 12
Bsu36I CC'TnA_GG
Cuts at: 1967 1967
Size: 5006
BtgI C'CryG_G
Cuts at: 107 251 107
Size: 144 4862
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2956 3137 3300 2956
Size: 181 163 4662
Fragments arranged by size:
4662 181 163
BtsI GCAGTG_nn'
Cuts at: 900 2249 900
Size: 1349 3657
DraI TTT'AAA
Cuts at: 2005 2005
Size: 5006
DrdI GACnn_nn'nnGTC
Cuts at: 2712 3570 2712
Size: 858 4148
EaeI y'GGCC_r
Cuts at: 401 719 1130 2591 2765 3156 3183 4528 401
Size: 318 411 1461 174 391 27 1345 879
Fragments arranged by size:
1461 1345 879 411 391 318 174 27
EagI C'GGCC_G
Cuts at: 2591 2591
Size: 5006
EarI CTCTTCn'nnn_
Cuts at: 166 484 673 854 1133 1493 1502 1577 3029
Size: 318 189 181 279 360 9 75 1452
Cuts at: 3029 3239 166
Size: 210 1933
Fragments arranged by size:
1933 1452 360 318 279 210 189 181
75 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 454 1330 3214 3524 3670 4816 454
Size: 876 1884 310 146 1146 644
Fragments arranged by size:
1884 1146 876 644 310 146
EcoICRI GAG'CTC
Cuts at: 666 744 2018 666
Size: 78 1274 3654
Fragments arranged by size:
3654 1274 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 280 613 682 781 1102 1219 1375 1420 1537
Size: 333 69 99 321 117 156 45 117
Cuts at: 1537 1555 1663 1796 2831 3263 4010 4278 280
Size: 18 108 133 1035 432 747 268 1008
Fragments arranged by size:
1035 1008 747 432 333 321 268 156
133 117 117 108 99 69 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 118 340 493 763 1072 1624 118
Size: 222 153 270 309 552 3500
Fragments arranged by size:
3500 552 309 270 222 153
EcoO109I rG'GnC_Cy
Cuts at: 1077 1428 1889 2145 1077
Size: 351 461 256 3938
Fragments arranged by size:
3938 461 351 256
EcoRI G'AATT_C
Cuts at: 2010 2010
Size: 5006
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3025 3025
Size: 5006
FspI TGC'GCA
Cuts at: 118 2787 118
Size: 2669 2337
HaeII r_GCGC'y
Cuts at: 942 989 1008 1125 1263 1294 1554 1860 2440
Size: 47 19 117 138 31 260 306 580
Cuts at: 2440 2688 3710 4689 942
Size: 248 1022 979 1259
Fragments arranged by size:
1259 1022 979 580 306 260 248 138
117 47 31 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1450 1482 2848 2878 2880 2910 1450
Size: 32 1366 30 2 30 3546
Fragments arranged by size:
3546 1366 32 30 30 2
HincII GTy'rAC
Cuts at: 2045 2045
Size: 5006
KasI G'GCGC_C
Cuts at: 1121 1259 2436 2684 1121
Size: 138 1177 248 3443
Fragments arranged by size:
3443 1177 248 138
KpnI G_GTAC'C
Cuts at: 2026 2026
Size: 5006
MfeI C'AATT_G
Cuts at: 1766 1766
Size: 5006
MluI A'CGCG_T
Cuts at: 4439 4439
Size: 5006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3677 3861 3677
Size: 184 4822
MscI TGG'CCA
Cuts at: 721 2767 721
Size: 2046 2960
MslI CAynn'nnrTG
Cuts at: 377 1030 1723 1736 2995 3122 377
Size: 653 693 13 1259 127 2261
Fragments arranged by size:
2261 1259 693 653 127 13
MspA1I CmG'CkG
Cuts at: 683 825 836 891 1021 1104 1113 1164 1221
Size: 142 11 55 130 83 9 51 57
Cuts at: 1221 1380 1416 1533 1641 1868 2117 2431 2791
Size: 159 36 117 108 227 249 314 360
Cuts at: 2791 3804 4049 683
Size: 1013 245 1640
Fragments arranged by size:
1640 1013 360 314 249 245 227 159
142 130 117 108 83 57 55 51
36 11 9
NaeI GCC'GGC
Cuts at: 3187 3187
Size: 5006
NarI GG'CG_CC
Cuts at: 1122 1260 2437 2685 1122
Size: 138 1177 248 3443
Fragments arranged by size:
3443 1177 248 138
NcoI C'CATG_G
Cuts at: 107 107
Size: 5006
NgoMIV G'CCGG_C
Cuts at: 3185 3185
Size: 5006
NsiI A_TGCA'T
Cuts at: 1763 1763
Size: 5006
NspI r_CATG'y
Cuts at: 315 948 2059 2559 3466 315
Size: 633 1111 500 907 1855
Fragments arranged by size:
1855 1111 907 633 500
PasI CC'CwG_GG
Cuts at: 1878 1878
Size: 5006
PciI A'CATG_T
Cuts at: 3462 3462
Size: 5006
PfoI T'CCnGG_A
Cuts at: 335 659 335
Size: 324 4682
PmeI GTTT'AAAC
Cuts at: 2005 2005
Size: 5006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 4145
Size: 32 4974
PpuMI rG'GwC_Cy
Cuts at: 1077 1889 1077
Size: 812 4194
PspXI vC'TCGA_Gb
Cuts at: 1294 1294
Size: 5006
PstI C_TGCA'G
Cuts at: 830 1049 1160 1169 1193 1211 1244 1286 1592
Size: 219 111 9 24 18 33 42 306
Cuts at: 1592 1625 2053 2738 830
Size: 33 428 685 3098
Fragments arranged by size:
3098 685 428 306 219 111 42 33
33 24 18 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5006
PvuII CAG'CTG
Cuts at: 825 836 1021 1164 1533 1641 2431 2791 825
Size: 11 185 143 369 108 790 360 3040
Fragments arranged by size:
3040 790 369 360 185 143 108 11
RsrII CG'GwC_CG
Cuts at: 3201 3201
Size: 5006
SacI G_AGCT'C
Cuts at: 668 746 2020 668
Size: 78 1274 3654
Fragments arranged by size:
3654 1274 78
SalI G'TCGA_C
Cuts at: 2043 2043
Size: 5006
SapI GCTCTTCn'nnn_
Cuts at: 673 1577 3029 3239 673
Size: 904 1452 210 2440
Fragments arranged by size:
2440 1452 904 210
SbfI CC_TGCA'GG
Cuts at: 2053 2053
Size: 5006
ScaI AGT'ACT
Cuts at: 4523 4523
Size: 5006
SfcI C'TryA_G
Cuts at: 33 826 1045 1156 1165 1189 1207 1240 1282
Size: 793 219 111 9 24 18 33 42
Cuts at: 1282 1588 1621 2049 2734 3727 3918 33
Size: 306 33 428 685 993 191 1121
Fragments arranged by size:
1121 993 793 685 428 306 219 191
111 42 33 33 24 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 613 622 613
Size: 9 4997
SfoI GGC'GCC
Cuts at: 1123 1261 2438 2686 1123
Size: 138 1177 248 3443
Fragments arranged by size:
3443 1177 248 138
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5006
SmaI CCC'GGG
Cuts at: 1327 2028 1327
Size: 701 4305
SmlI C'TyrA_G
Cuts at: 130 199 1294 2161 3076 3568 3830 130
Size: 69 1095 867 915 492 262 1306
Fragments arranged by size:
1306 1095 915 867 492 262 69
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5006
SphI G_CATG'C
Cuts at: 2059 2559 2059
Size: 500 4506
SrfI GCCC'GGGC
Cuts at: 1327 1327
Size: 5006
StyI C'CwwG_G
Cuts at: 107 1133 2140 2494 107
Size: 1026 1007 354 2619
Fragments arranged by size:
2619 1026 1007 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 574 2589 2722 3181 3390 574
Size: 2015 133 459 209 2190
Fragments arranged by size:
2190 2015 459 209 133
TatI w'GTAC_w
Cuts at: 221 329 4521 221
Size: 108 4192 706
Fragments arranged by size:
4192 706 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1828 1860 4523 4555 1828
Size: 32 2663 32 2279
Fragments arranged by size:
2663 2279 32 32
Tth111I GACn'n_nGTC
Cuts at: 2803 2803
Size: 5006
XbaI T'CTAG_A
Cuts at: 2037 2037
Size: 5006
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2501 2501
Size: 5006
XhoI C'TCGA_G
Cuts at: 1294 1294
Size: 5006
XmaI C'CCGG_G
Cuts at: 1325 2026 1325
Size: 701 4305
ZraI GAC'GTC
Cuts at: 1064 1064
Size: 5006
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PciI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI
Enzymes that do not cut:
AarI AflII AhdI ApaI AscI AvrII BaeI BmtI
BsiWI BsmI BsmBI BssHII BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI PacI PflMI PmlI PshAI PsiI PspOMI PsrI
SacII SanDI SexAI SgrAI SnaBI SspI StuI SwaI
XmnI