Restriction map for
pF1KB6135
(Circular) (Six-base) MAPSORT of: pF1KB6135.seq Check: 1358 from: 1 to: 4889
pF1KB6135 4889 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 692 1927 692
Size: 1235 3654
Acc65I G'GTAC_C
Cuts at: 1905 1905
Size: 4889
AclI AA'CG_TT
Cuts at: 1250 4729 1250
Size: 3479 1410
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1258 2714 3146 3893 4161 1258
Size: 1456 432 747 268 1986
Fragments arranged by size:
1986 1456 747 432 268
AfeI AGC'GCT
Cuts at: 517 619 517
Size: 102 4787
AflII C'TTAA_G
Cuts at: 799 799
Size: 4889
AflIII A'CryG_T
Cuts at: 245 1013 1652 2241 3345 4322 245
Size: 768 639 589 1104 977 812
Fragments arranged by size:
1104 977 812 768 639 589
AgeI A'CCGG_T
Cuts at: 3277 3277
Size: 4889
AhdI GACnn_n'nnGTC
Cuts at: 1076 1644 1076
Size: 568 4321
AleI CACnn'nnGTG
Cuts at: 196 196
Size: 4889
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1108 1140 1378 1410 1108
Size: 32 238 32 4587
Fragments arranged by size:
4587 238 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2194 3761 2194
Size: 1567 3322
ApaI G_GGCC'C
Cuts at: 1318 1318
Size: 4889
ApaLI G'TGCA_C
Cuts at: 3659 3659
Size: 4889
ApoI r'AATT_y
Cuts at: 935 1893 4242 4498 935
Size: 958 2349 256 1326
Fragments arranged by size:
2349 1326 958 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4889
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
AvaI C'yCGr_G
Cuts at: 396 1909 396
Size: 1513 3376
BamHI G'GATC_C
Cuts at: 1535 1914 1535
Size: 379 4510
BanI G'GyrC_C
Cuts at: 1905 2319 2567 2602 1905
Size: 414 248 35 4192
Fragments arranged by size:
4192 414 248 35
BanII G_rGCy'C
Cuts at: 227 1318 1775 1903 2933 227
Size: 1091 457 128 1030 2183
Fragments arranged by size:
2183 1091 1030 457 128
BbeI G_GCGC'C
Cuts at: 2323 2571 2323
Size: 248 4641
BbsI GAAGACnn'nnnn_
Cuts at: 443 443
Size: 4889
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1783 1817 90
Size: 34 1659 34 3162
Fragments arranged by size:
3162 1659 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2787 3548 4889 2787
Size: 761 1341 2787
Fragments arranged by size:
2787 1341 761
BclI T'GATC_A
Cuts at: 2409 2409
Size: 4889
BglII A'GATC_T
Cuts at: 1184 2404 4441 1184
Size: 1220 2037 1632
Fragments arranged by size:
2037 1632 1220
BlpI GC'TnA_GC
Cuts at: 2001 2001
Size: 4889
Bme1580I G_kGCm'C
Cuts at: 1274 1318 2514 2607 3663 1274
Size: 44 1196 93 1056 2500
Fragments arranged by size:
2500 1196 1056 93 44
BmrI ACTGGGnnnn_n'
Cuts at: 1002 1683 2101 2182 2517 4661 1002
Size: 681 418 81 335 2144 1230
Fragments arranged by size:
2144 1230 681 418 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3256 3256
Size: 4889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1540 1705 671
Size: 869 165 3855
Fragments arranged by size:
3855 869 165
Bpu10I CC'TnA_GC
Cuts at: 407 407
Size: 4889
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 265 2065 2944 3436 3734 145
Size: 120 1800 879 492 298 1300
Fragments arranged by size:
1800 1300 879 492 298 120
BsaI GGTCTCn'nnnn_
Cuts at: 4593 4593
Size: 4889
BsaAI yAC'GTr
Cuts at: 1014 2242 1014
Size: 1228 3661
BsaBI GATnn'nnATC
Cuts at: 6 1189 2403 4440 6
Size: 1183 1214 2037 455
Fragments arranged by size:
2037 1214 1183 455
BsaHI Gr'CG_yC
Cuts at: 203 710 2320 2568 3270 4766 203
Size: 507 1610 248 702 1496 326
Fragments arranged by size:
1610 1496 702 507 326 248
BsaWI w'CCGG_w
Cuts at: 1532 2078 2599 3277 3551 3698 4109 1532
Size: 546 521 678 274 147 411 2312
Fragments arranged by size:
2312 678 546 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1378 1408 1378
Size: 30 4859
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 742 1171 1399 1888 235
Size: 507 429 228 489 3236
Fragments arranged by size:
3236 507 489 429 228
BseYI C'CCAG_C
Cuts at: 112 1416 2314 3072 3649 4145 112
Size: 1304 898 758 577 496 856
Fragments arranged by size:
1304 898 856 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 384 567 1192 1798 241
Size: 143 183 625 606 3332
Fragments arranged by size:
3332 625 606 183 143
BsiEI CG_ry'CG
Cuts at: 104 150 1181 2431 2477 3685 104
Size: 46 1031 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 1031 46 46
BsiHKAI G_wGCw'C
Cuts at: 227 403 1163 1336 1903 2681 2871 3663 227
Size: 176 760 173 567 778 190 792 1453
Fragments arranged by size:
1453 792 778 760 567 190 176 173
Bsp1286I G_dGCh'C
Cuts at: 227 403 1163 1274 1318 1336 1775 1903 2514
Size: 176 760 111 44 18 439 128 611
Cuts at: 2514 2607 2681 2871 2933 3663 227
Size: 93 74 190 62 730 1453
Fragments arranged by size:
1453 760 730 611 439 190 176 128
111 93 74 62 44 18
BspEI T'CCGG_A
Cuts at: 1532 4109 1532
Size: 2577 2312
BspHI T'CATG_A
Cuts at: 4065 4065
Size: 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 652 1939 2153 2455 2836 652
Size: 1287 214 302 381 2705
Fragments arranged by size:
2705 1287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 143 273 3181 3235 4719 4884 50
Size: 93 130 2908 54 1484 165 55
Fragments arranged by size:
2908 1484 165 130 93 55 54
BsrDI GCAATG_nn'
Cuts at: 2801 2801
Size: 4889
BsrFI r'CCGG_y
Cuts at: 121 1616 2887 3068 3277 121
Size: 1495 1271 181 209 1733
Fragments arranged by size:
1733 1495 1271 209 181
BssSI C'ACGA_G
Cuts at: 1210 3160 3518 1210
Size: 1950 358 2581
Fragments arranged by size:
2581 1950 358
BstAPI GCAn_nnn'nTGC
Cuts at: 596 1340 2266 596
Size: 744 926 3219
Fragments arranged by size:
3219 926 744
BstBI TT'CG_AA
Cuts at: 1832 3250 1832
Size: 1418 3471
BstEII G'GTnAC_C
Cuts at: 301 301
Size: 4889
BstXI CCAn_nnnn'nTGG
Cuts at: 951 951
Size: 4889
BstYI r'GATC_y
Cuts at: 1 1184 1535 1914 2381 2404 2739 2985 3997
Size: 1183 351 379 467 23 335 246 1012
Cuts at: 3997 4083 4095 4441 1
Size: 86 12 346 449
Fragments arranged by size:
1183 1012 467 449 379 351 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1154 1154
Size: 4889
BtgI C'CryG_G
Cuts at: 624 624
Size: 4889
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1176 1344 2839 3020 3183 1176
Size: 168 1495 181 163 2882
Fragments arranged by size:
2882 1495 181 168 163
BtsI GCAGTG_nn'
Cuts at: 2132 2132
Size: 4889
ClaI AT'CG_AT
Cuts at: 812 812
Size: 4889
DraI TTT'AAA
Cuts at: 1574 1586 1888 1574
Size: 12 302 4575
Fragments arranged by size:
4575 302 12
DrdI GACnn_nn'nnGTC
Cuts at: 2595 3453 2595
Size: 858 4031
EaeI y'GGCC_r
Cuts at: 147 495 1178 1442 1618 2474 2648 3039 3066
Size: 348 683 264 176 856 174 391 27
Cuts at: 3066 4411 147
Size: 1345 625
Fragments arranged by size:
1345 856 683 625 391 348 264 176
174 27
EagI C'GGCC_G
Cuts at: 147 1178 2474 147
Size: 1031 1296 2562
Fragments arranged by size:
2562 1296 1031
EarI CTCTTCn'nnn_
Cuts at: 332 508 2912 3122 332
Size: 176 2404 210 2099
Fragments arranged by size:
2404 2099 210 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 145 337 3097 3407 3553 4699 145
Size: 192 2760 310 146 1146 335
Fragments arranged by size:
2760 1146 335 310 192 146
EcoICRI GAG'CTC
Cuts at: 225 1901 225
Size: 1676 3213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 671 1258 1540 1705 2714 3146 3893 4161 671
Size: 587 282 165 1009 432 747 268 1399
Fragments arranged by size:
1399 1009 747 587 432 282 268 165
EcoO109I rG'GnC_Cy
Cuts at: 564 795 1116 1233 1315 1593 2028 564
Size: 231 321 117 82 278 435 3425
Fragments arranged by size:
3425 435 321 278 231 117 82
EcoRI G'AATT_C
Cuts at: 1893 1893
Size: 4889
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2908 2908
Size: 4889
FspI TGC'GCA
Cuts at: 2670 2670
Size: 4889
HaeII r_GCGC'y
Cuts at: 519 621 1417 1452 1702 2323 2571 3593 4572
Size: 102 796 35 250 621 248 1022 979
Cuts at: 4572 519
Size: 836
Fragments arranged by size:
1022 979 836 796 621 250 248 102
35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1012 1044 1062 1094 1630 1662 2731 2761 2763
Size: 32 18 32 536 32 1069 30 2
Cuts at: 2763 2793 1012
Size: 30 3108
Fragments arranged by size:
3108 1069 536 32 32 32 30 30
18 2
HincII GTy'rAC
Cuts at: 1107 1928 1107
Size: 821 4068
KasI G'GCGC_C
Cuts at: 2319 2567 2319
Size: 248 4641
KpnI G_GTAC'C
Cuts at: 1909 1909
Size: 4889
MluI A'CGCG_T
Cuts at: 1652 4322 1652
Size: 2670 2219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1029 3560 3744 1029
Size: 2531 184 2174
Fragments arranged by size:
2531 2174 184
MscI TGG'CCA
Cuts at: 2650 2650
Size: 4889
MslI CAynn'nnrTG
Cuts at: 196 949 2878 3005 196
Size: 753 1929 127 2080
Fragments arranged by size:
2080 1929 753 127
MspA1I CmG'CkG
Cuts at: 639 1782 2000 2314 2674 3687 3932 639
Size: 1143 218 314 360 1013 245 1596
Fragments arranged by size:
1596 1143 1013 360 314 245 218
NaeI GCC'GGC
Cuts at: 1618 3070 1618
Size: 1452 3437
NarI GG'CG_CC
Cuts at: 2320 2568 2320
Size: 248 4641
NdeI CA'TA_TG
Cuts at: 656 656
Size: 4889
NgoMIV G'CCGG_C
Cuts at: 1616 3068 1616
Size: 1452 3437
NotI GC'GGCC_GC
Cuts at: 147 147
Size: 4889
NruI TCG'CGA
Cuts at: 571 571
Size: 4889
NspI r_CATG'y
Cuts at: 249 1134 1299 1942 2442 3349 249
Size: 885 165 643 500 907 1789
Fragments arranged by size:
1789 907 885 643 500 165
PciI A'CATG_T
Cuts at: 245 3345 245
Size: 3100 1789
PflMI CCAn_nnn'nTGG
Cuts at: 470 1627 470
Size: 1157 3732
PfoI T'CCnGG_A
Cuts at: 429 760 1256 1517 429
Size: 331 496 261 3801
Fragments arranged by size:
3801 496 331 261
PmeI GTTT'AAAC
Cuts at: 1888 1888
Size: 4889
PmlI CAC'GTG
Cuts at: 1014 1014
Size: 4889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1378 1410 4028 4060 1378
Size: 32 2618 32 2207
Fragments arranged by size:
2618 2207 32 32
PpuMI rG'GwC_Cy
Cuts at: 795 1116 1233 1593 795
Size: 321 117 360 4091
Fragments arranged by size:
4091 360 321 117
PshAI GACnn'nnGTC
Cuts at: 248 1736 248
Size: 1488 3401
PspOMI G'GGCC_C
Cuts at: 1314 1314
Size: 4889
PstI C_TGCA'G
Cuts at: 649 857 1936 2621 649
Size: 208 1079 685 2917
Fragments arranged by size:
2917 1079 685 208
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4889
PvuII CAG'CTG
Cuts at: 1782 2314 2674 1782
Size: 532 360 3997
Fragments arranged by size:
3997 532 360
RsrII CG'GwC_CG
Cuts at: 3084 3084
Size: 4889
SacI G_AGCT'C
Cuts at: 227 1903 227
Size: 1676 3213
SalI G'TCGA_C
Cuts at: 1926 1926
Size: 4889
SapI GCTCTTCn'nnn_
Cuts at: 2912 3122 2912
Size: 210 4679
SbfI CC_TGCA'GG
Cuts at: 649 1936 649
Size: 1287 3602
ScaI AGT'ACT
Cuts at: 4406 4406
Size: 4889
SfcI C'TryA_G
Cuts at: 33 582 645 853 1227 1932 2617 3610 3801
Size: 549 63 208 374 705 685 993 191
Cuts at: 3801 33
Size: 1121
Fragments arranged by size:
1121 993 705 685 549 374 208 191
63
SfoI GGC'GCC
Cuts at: 2321 2569 2321
Size: 248 4641
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4889
SmaI CCC'GGG
Cuts at: 1911 1911
Size: 4889
SmlI C'TyrA_G
Cuts at: 160 280 799 2044 2959 3451 3713 160
Size: 120 519 1245 915 492 262 1336
Fragments arranged by size:
1336 1245 915 519 492 262 120
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4889
SphI G_CATG'C
Cuts at: 1942 2442 1942
Size: 500 4389
StuI AGG'CCT
Cuts at: 377 1310 377
Size: 933 3956
StyI C'CwwG_G
Cuts at: 899 2023 2377 899
Size: 1124 354 3411
Fragments arranged by size:
3411 1124 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 276 2472 2605 3064 3273 276
Size: 2196 133 459 209 1892
Fragments arranged by size:
2196 1892 459 209 133
TatI w'GTAC_w
Cuts at: 4404 4404
Size: 4889
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4406 4438 4406
Size: 32 4857
Tth111I GACn'n_nGTC
Cuts at: 2686 2686
Size: 4889
XbaI T'CTAG_A
Cuts at: 1920 1920
Size: 4889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 906 2384 906
Size: 1478 3411
XmaI C'CCGG_G
Cuts at: 1909 1909
Size: 4889
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NotI NruI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AlfI AscI AvrII BaeI BbvCI BfrBI
BglI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NheI NsiI PacI PasI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI