Restriction map for
pF1KB6136
(Circular) (Six-base) MAPSORT of: pF1KB6136.seq Check: 1897 from: 1 to: 5030
pF1KB6136 5030 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 774 1440 774
Size: 666 4364
AccI GT'mk_AC
Cuts at: 2068 2068
Size: 5030
Acc65I G'GTAC_C
Cuts at: 1076 1234 2046 1076
Size: 158 812 4060
Fragments arranged by size:
4060 812 158
AclI AA'CG_TT
Cuts at: 4870 4870
Size: 5030
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 566 1129 1457 2855 3287 4034 4302 302
Size: 264 563 328 1398 432 747 268 1030
Fragments arranged by size:
1398 1030 747 563 432 328 268 264
AflIII A'CryG_T
Cuts at: 2382 3486 4463 2382
Size: 1104 977 2949
Fragments arranged by size:
2949 1104 977
AgeI A'CCGG_T
Cuts at: 3418 3418
Size: 5030
AhdI GACnn_n'nnGTC
Cuts at: 561 1053 561
Size: 492 4538
AlwNI CAG_nnn'CTG
Cuts at: 1230 1735 2335 3902 1230
Size: 505 600 1567 2358
Fragments arranged by size:
2358 1567 600 505
ApaI G_GGCC'C
Cuts at: 465 1468 1752 1882 465
Size: 1003 284 130 3613
Fragments arranged by size:
3613 1003 284 130
ApaLI G'TGCA_C
Cuts at: 3800 3800
Size: 5030
ApoI r'AATT_y
Cuts at: 301 535 757 2034 4383 4639 301
Size: 234 222 1277 2349 256 692
Fragments arranged by size:
2349 1277 692 256 234 222
AseI AT'TA_AT
Cuts at: 20 20
Size: 5030
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
AvaI C'yCGr_G
Cuts at: 1753 1813 1883 2050 1753
Size: 60 70 167 4733
Fragments arranged by size:
4733 167 70 60
BamHI G'GATC_C
Cuts at: 1200 2055 1200
Size: 855 4175
BanI G'GyrC_C
Cuts at: 145 1076 1139 1234 1693 1897 2046 2460 2708
Size: 931 63 95 459 204 149 414 248
Cuts at: 2708 2743 145
Size: 35 2432
Fragments arranged by size:
2432 931 459 414 248 204 149 95
63 35
BanII G_rGCy'C
Cuts at: 240 426 465 822 1468 1529 1752 1760 1821
Size: 186 39 357 646 61 223 8 61
Cuts at: 1821 1882 2044 3074 240
Size: 61 162 1030 2196
Fragments arranged by size:
2196 1030 646 357 223 186 162 61
61 61 39 8
BbeI G_GCGC'C
Cuts at: 1143 1697 2464 2712 1143
Size: 554 767 248 3461
Fragments arranged by size:
3461 767 554 248
BbvCI CC'TCA_GC
Cuts at: 587 1016 1115 1651 587
Size: 429 99 536 3966
Fragments arranged by size:
3966 536 429 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1794 1828 1794
Size: 34 4996
BciVI GTATCCnnnnn_n'
Cuts at: 1489 2928 3689 5030 1489
Size: 1439 761 1341 1489
Fragments arranged by size:
1489 1439 1341 761
BclI T'GATC_A
Cuts at: 1107 2550 1107
Size: 1443 3587
BglI GCCn_nnn'nGGC
Cuts at: 250 583 1702 1969 250
Size: 333 1119 267 3311
Fragments arranged by size:
3311 1119 333 267
BglII A'GATC_T
Cuts at: 2545 4582 2545
Size: 2037 2993
BlpI GC'TnA_GC
Cuts at: 2142 2142
Size: 5030
Bme1580I G_kGCm'C
Cuts at: 176 342 465 1468 1752 1882 2655 2748 3804
Size: 166 123 1003 284 130 773 93 1056
Cuts at: 3804 176
Size: 1402
Fragments arranged by size:
1402 1056 1003 773 284 166 130 123
93
BmrI ACTGGGnnnn_n'
Cuts at: 468 1471 2242 2323 2658 4802 468
Size: 1003 771 81 335 2144 696
Fragments arranged by size:
2144 1003 771 696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 3397 273
Size: 3124 1906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 814 1315 1744 1867 1938 503
Size: 311 501 429 123 71 3595
Fragments arranged by size:
3595 501 429 311 123 71
Bpu10I CC'TnA_GC
Cuts at: 587 821 1016 1115 1205 1651 587
Size: 234 195 99 90 446 3966
Fragments arranged by size:
3966 446 234 195 99 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2206 3085 3577 3875 2206
Size: 879 492 298 3361
Fragments arranged by size:
3361 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4734 4734
Size: 5030
BsaAI yAC'GTr
Cuts at: 2383 2383
Size: 5030
BsaBI GATnn'nnATC
Cuts at: 6 668 2544 4581 6
Size: 662 1876 2037 455
Fragments arranged by size:
2037 1876 662 455
BsaHI Gr'CG_yC
Cuts at: 1140 1694 2461 2709 3411 4907 1140
Size: 554 767 248 702 1496 1263
Fragments arranged by size:
1496 1263 767 702 554 248
BsaWI w'CCGG_w
Cuts at: 799 1775 2219 2740 3418 3692 3839 4250 799
Size: 976 444 521 678 274 147 411 1579
Fragments arranged by size:
1579 976 678 521 444 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 476 506 604 634 1543 1573 476
Size: 30 98 30 909 30 3933
Fragments arranged by size:
3933 909 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 625 694 1009 1102 1105 625
Size: 69 315 93 3 4550
Fragments arranged by size:
4550 315 93 69 3
BseYI C'CCAG_C
Cuts at: 581 624 1468 2455 3213 3790 4286 581
Size: 43 844 987 758 577 496 1325
Fragments arranged by size:
1325 987 844 758 577 496 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 824 1213 1597 824
Size: 389 384 4257
Fragments arranged by size:
4257 389 384
BsiEI CG_ry'CG
Cuts at: 104 654 2572 2618 3826 104
Size: 550 1918 46 1208 1308
Fragments arranged by size:
1918 1308 1208 550 46
BsiHKAI G_wGCw'C
Cuts at: 822 1529 1760 1841 1924 2044 2822 3012 3804
Size: 707 231 81 83 120 778 190 792
Cuts at: 3804 822
Size: 2048
Fragments arranged by size:
2048 792 778 707 231 190 120 83
81
BsmI GAATG_Cn'
Cuts at: 142 324 142
Size: 182 4848
Bsp1286I G_dGCh'C
Cuts at: 176 240 342 426 465 822 1468 1529 1752
Size: 64 102 84 39 357 646 61 223
Cuts at: 1752 1760 1821 1841 1882 1924 2044 2655 2748
Size: 8 61 20 41 42 120 611 93
Cuts at: 2748 2822 3012 3074 3804 176
Size: 74 190 62 730 1402
Fragments arranged by size:
1402 730 646 611 357 223 190 120
102 93 84 74 64 62 61 61
42 41 39 20 8
BspEI T'CCGG_A
Cuts at: 4250 4250
Size: 5030
BspHI T'CATG_A
Cuts at: 158 4206 158
Size: 4048 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 774 1166 1440 2080 2294 2596 2977 774
Size: 392 274 640 214 302 381 2827
Fragments arranged by size:
2827 640 392 381 302 274 214
BsrBI CCG'CTC
Cuts at: 50 156 3322 3376 4860 5025 50
Size: 106 3166 54 1484 165 55
Fragments arranged by size:
3166 1484 165 106 55 54
BsrDI GCAATG_nn'
Cuts at: 119 1279 2942 119
Size: 1160 1663 2207
Fragments arranged by size:
2207 1663 1160
BsrFI r'CCGG_y
Cuts at: 3028 3209 3418 3028
Size: 181 209 4640
Fragments arranged by size:
4640 209 181
BssSI C'ACGA_G
Cuts at: 3301 3659 3301
Size: 358 4672
BstAPI GCAn_nnn'nTGC
Cuts at: 1735 1802 2407 1735
Size: 67 605 4358
Fragments arranged by size:
4358 605 67
BstBI TT'CG_AA
Cuts at: 3391 3391
Size: 5030
BstXI CCAn_nnnn'nTGG
Cuts at: 267 267
Size: 5030
BstYI r'GATC_y
Cuts at: 1 684 1200 1486 1986 2055 2522 2545 2880
Size: 683 516 286 500 69 467 23 335
Cuts at: 2880 3126 4138 4224 4236 4582 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 683 516 500 467 449 346 335
286 246 86 69 23 12
Bsu36I CC'TnA_GG
Cuts at: 635 677 635
Size: 42 4988
BtgI C'CryG_G
Cuts at: 107 1628 107
Size: 1521 3509
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1973 2980 3161 3324 1973
Size: 1007 181 163 3679
Fragments arranged by size:
3679 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 1585 2273 1585
Size: 688 4342
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1590 1625 1590
Size: 35 4995
DraI TTT'AAA
Cuts at: 2029 2029
Size: 5030
DrdI GACnn_nn'nnGTC
Cuts at: 525 2736 3594 525
Size: 2211 858 1961
Fragments arranged by size:
2211 1961 858
EaeI y'GGCC_r
Cuts at: 698 713 1703 2615 2789 3180 3207 4552 698
Size: 15 990 912 174 391 27 1345 1176
Fragments arranged by size:
1345 1176 990 912 391 174 27 15
EagI C'GGCC_G
Cuts at: 2615 2615
Size: 5030
EarI CTCTTCn'nnn_
Cuts at: 1534 3053 3263 1534
Size: 1519 210 3301
Fragments arranged by size:
3301 1519 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 394 3238 3548 3694 4840 394
Size: 2844 310 146 1146 584
Fragments arranged by size:
2844 1146 584 310 146
EcoICRI GAG'CTC
Cuts at: 820 1527 1758 2042 820
Size: 707 231 284 3808
Fragments arranged by size:
3808 707 284 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 503 566 814 1129 1315 1457 1744 1867
Size: 201 63 248 315 186 142 287 123
Cuts at: 1867 1938 2855 3287 4034 4302 302
Size: 71 917 432 747 268 1030
Fragments arranged by size:
1030 917 747 432 315 287 268 248
201 186 142 123 71 63
EcoNI CCTnn'n_nnAGG
Cuts at: 199 1147 2020 199
Size: 948 873 3209
Fragments arranged by size:
3209 948 873
EcoO109I rG'GnC_Cy
Cuts at: 192 1155 1465 1749 1851 1878 1879 2169 192
Size: 963 310 284 102 27 1 290 3053
Fragments arranged by size:
3053 963 310 290 284 102 27 1
EcoRI G'AATT_C
Cuts at: 301 535 2034 301
Size: 234 1499 3297
Fragments arranged by size:
3297 1499 234
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3049 3049
Size: 5030
FspI TGC'GCA
Cuts at: 2811 2811
Size: 5030
HaeII r_GCGC'y
Cuts at: 1143 1333 1697 2464 2712 3734 4713 1143
Size: 190 364 767 248 1022 979 1460
Fragments arranged by size:
1460 1022 979 767 364 248 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1039 1071 1543 1575 2872 2902 2904
Size: 32 460 32 472 32 1297 30 2
Cuts at: 2904 2934 547
Size: 30 2643
Fragments arranged by size:
2643 1297 472 460 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 882 2069 882
Size: 1187 3843
HindIII A'AGCT_T
Cuts at: 1217 1217
Size: 5030
KasI G'GCGC_C
Cuts at: 1139 1693 2460 2708 1139
Size: 554 767 248 3461
Fragments arranged by size:
3461 767 554 248
KpnI G_GTAC'C
Cuts at: 1080 1238 2050 1080
Size: 158 812 4060
Fragments arranged by size:
4060 812 158
MluI A'CGCG_T
Cuts at: 4463 4463
Size: 5030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 971 1886 3701 3885 971
Size: 915 1815 184 2116
Fragments arranged by size:
2116 1815 915 184
MscI TGG'CCA
Cuts at: 715 1705 2791 715
Size: 990 1086 2954
Fragments arranged by size:
2954 1086 990
MslI CAynn'nnrTG
Cuts at: 112 3019 3146 112
Size: 2907 127 1996
Fragments arranged by size:
2907 1996 127
MspA1I CmG'CkG
Cuts at: 481 581 1189 1540 1645 1802 2141 2455 2815
Size: 100 608 351 105 157 339 314 360
Cuts at: 2815 3828 4073 481
Size: 1013 245 1438
Fragments arranged by size:
1438 1013 608 360 351 339 314 245
157 105 100
NaeI GCC'GGC
Cuts at: 3211 3211
Size: 5030
NarI GG'CG_CC
Cuts at: 1140 1694 2461 2709 1140
Size: 554 767 248 3461
Fragments arranged by size:
3461 767 554 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5030
NgoMIV G'CCGG_C
Cuts at: 3209 3209
Size: 5030
NspI r_CATG'y
Cuts at: 1863 2083 2583 3490 1863
Size: 220 500 907 3403
Fragments arranged by size:
3403 907 500 220
PciI A'CATG_T
Cuts at: 3486 3486
Size: 5030
PmeI GTTT'AAAC
Cuts at: 2029 2029
Size: 5030
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 4169
Size: 32 4998
PpuMI rG'GwC_Cy
Cuts at: 1155 1155
Size: 5030
PsiI TTA'TAA
Cuts at: 512 512
Size: 5030
PspOMI G'GGCC_C
Cuts at: 461 1464 1748 1878 461
Size: 1003 284 130 3613
Fragments arranged by size:
3613 1003 284 130
PspXI vC'TCGA_Gb
Cuts at: 1753 1753
Size: 5030
PstI C_TGCA'G
Cuts at: 311 692 1364 2077 2762 311
Size: 381 672 713 685 2579
Fragments arranged by size:
2579 713 685 672 381
PvuI CG_AT'CG
Cuts at: 104 654 104
Size: 550 4480
PvuII CAG'CTG
Cuts at: 481 1189 1540 1645 1802 2455 2815 481
Size: 708 351 105 157 653 360 2696
Fragments arranged by size:
2696 708 653 360 351 157 105
RsrII CG'GwC_CG
Cuts at: 3225 3225
Size: 5030
SacI G_AGCT'C
Cuts at: 822 1529 1760 2044 822
Size: 707 231 284 3808
Fragments arranged by size:
3808 707 284 231
SalI G'TCGA_C
Cuts at: 2067 2067
Size: 5030
SapI GCTCTTCn'nnn_
Cuts at: 1534 3053 3263 1534
Size: 1519 210 3301
Fragments arranged by size:
3301 1519 210
SbfI CC_TGCA'GG
Cuts at: 311 2077 311
Size: 1766 3264
ScaI AGT'ACT
Cuts at: 4547 4547
Size: 5030
SfcI C'TryA_G
Cuts at: 33 307 688 947 1360 2073 2758 3751 3942
Size: 274 381 259 413 713 685 993 191
Cuts at: 3942 33
Size: 1121
Fragments arranged by size:
1121 993 713 685 413 381 274 259
191
SfoI GGC'GCC
Cuts at: 1141 1695 2462 2710 1141
Size: 554 767 248 3461
Fragments arranged by size:
3461 767 554 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5030
SmaI CCC'GGG
Cuts at: 1885 2052 1885
Size: 167 4863
SmlI C'TyrA_G
Cuts at: 1753 2185 3100 3592 3854 1753
Size: 432 915 492 262 2929
Fragments arranged by size:
2929 915 492 432 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5030
SphI G_CATG'C
Cuts at: 2083 2583 2083
Size: 500 4530
SspI AAT'ATT
Cuts at: 595 595
Size: 5030
StuI AGG'CCT
Cuts at: 216 1372 216
Size: 1156 3874
StyI C'CwwG_G
Cuts at: 107 2164 2518 107
Size: 2057 354 2619
Fragments arranged by size:
2619 2057 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 1255 2613 2746 3205 3414 196
Size: 1059 1358 133 459 209 1812
Fragments arranged by size:
1812 1358 1059 459 209 133
TatI w'GTAC_w
Cuts at: 4545 4545
Size: 5030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 476 508 604 636 4547 4579 476
Size: 32 96 32 3911 32 927
Fragments arranged by size:
3911 927 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2827 2827
Size: 5030
XbaI T'CTAG_A
Cuts at: 2061 2061
Size: 5030
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 424 708 2525 424
Size: 284 1817 2929
Fragments arranged by size:
2929 1817 284
XhoI C'TCGA_G
Cuts at: 1753 1753
Size: 5030
XmaI C'CCGG_G
Cuts at: 1883 2050 1883
Size: 167 4863
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI