Restriction map for
pF1KB6139
(Circular) (Six-base) MAPSORT of: pF1KB6139.seq Check: 9325 from: 1 to: 4892
pF1KB6139 4892 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 442 1154 442
Size: 712 4180
AccI GT'mk_AC
Cuts at: 1930 1930
Size: 4892
Acc65I G'GTAC_C
Cuts at: 1908 1908
Size: 4892
AclI AA'CG_TT
Cuts at: 4732 4732
Size: 4892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 1867 2717 3149 3896 4164 145
Size: 1722 850 432 747 268 873
Fragments arranged by size:
1722 873 850 747 432 268
AflIII A'CryG_T
Cuts at: 2244 3348 4325 2244
Size: 1104 977 2811
Fragments arranged by size:
2811 1104 977
AgeI A'CCGG_T
Cuts at: 3280 3280
Size: 4892
AhdI GACnn_n'nnGTC
Cuts at: 825 825
Size: 4892
AlwNI CAG_nnn'CTG
Cuts at: 366 798 1095 1218 2197 3764 366
Size: 432 297 123 979 1567 1494
Fragments arranged by size:
1567 1494 979 432 297 123
ApaI G_GGCC'C
Cuts at: 691 775 1702 691
Size: 84 927 3881
Fragments arranged by size:
3881 927 84
ApaLI G'TGCA_C
Cuts at: 3662 3662
Size: 4892
ApoI r'AATT_y
Cuts at: 146 1896 4245 4501 146
Size: 1750 2349 256 537
Fragments arranged by size:
2349 1750 537 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4892
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
AvaI C'yCGr_G
Cuts at: 117 439 613 1459 1467 1691 1912 117
Size: 322 174 846 8 224 221 3097
Fragments arranged by size:
3097 846 322 224 221 174 8
BamHI G'GATC_C
Cuts at: 1917 1917
Size: 4892
BanI G'GyrC_C
Cuts at: 111 1299 1908 2322 2570 2605 111
Size: 1188 609 414 248 35 2398
Fragments arranged by size:
2398 1188 609 414 248 35
BanII G_rGCy'C
Cuts at: 343 446 691 775 848 1025 1186 1231 1468
Size: 103 245 84 73 177 161 45 237
Cuts at: 1468 1511 1702 1906 2936 343
Size: 43 191 204 1030 2299
Fragments arranged by size:
2299 1030 245 237 204 191 177 161
103 84 73 45 43
BbeI G_GCGC'C
Cuts at: 115 2326 2574 115
Size: 2211 248 2433
Fragments arranged by size:
2433 2211 248
BbsI GAAGACnn'nnnn_
Cuts at: 224 290 496 224
Size: 66 206 4620
Fragments arranged by size:
4620 206 66
BbvCI CC'TCA_GC
Cuts at: 263 263
Size: 4892
BciVI GTATCCnnnnn_n'
Cuts at: 1614 2790 3551 4892 1614
Size: 1176 761 1341 1614
Fragments arranged by size:
1614 1341 1176 761
BclI T'GATC_A
Cuts at: 368 2412 368
Size: 2044 2848
BfrBI ATG'CAT
Cuts at: 990 990
Size: 4892
BglI GCCn_nnn'nGGC
Cuts at: 120 709 724 745 1621 120
Size: 589 15 21 876 3391
Fragments arranged by size:
3391 876 589 21 15
BglII A'GATC_T
Cuts at: 2407 4444 2407
Size: 2037 2855
BlpI GC'TnA_GC
Cuts at: 337 919 2004 337
Size: 582 1085 3225
Fragments arranged by size:
3225 1085 582
Bme1580I G_kGCm'C
Cuts at: 691 775 1304 1650 1702 2517 2610 3666 691
Size: 84 529 346 52 815 93 1056 1917
Fragments arranged by size:
1917 1056 815 529 346 93 84 52
BmrI ACTGGGnnnn_n'
Cuts at: 308 699 752 2104 2185 2520 4664 308
Size: 391 53 1352 81 335 2144 536
Fragments arranged by size:
2144 1352 536 391 335 81 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3259 3259
Size: 4892
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 1450 1461 1507 1888 340
Size: 1110 11 46 381 3344
Fragments arranged by size:
3344 1110 381 46 11
Bpu10I CC'TnA_GC
Cuts at: 263 263
Size: 4892
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2068 2947 3439 3737 2068
Size: 879 492 298 3223
Fragments arranged by size:
3223 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 901 4596 901
Size: 3695 1197
BsaAI yAC'GTr
Cuts at: 2245 2245
Size: 4892
BsaBI GATnn'nnATC
Cuts at: 6 2406 4443 6
Size: 2400 2037 455
Fragments arranged by size:
2400 2037 455
BsaHI Gr'CG_yC
Cuts at: 112 1562 2323 2571 3273 4769 112
Size: 1450 761 248 702 1496 235
Fragments arranged by size:
1496 1450 761 702 248 235
BsaWI w'CCGG_w
Cuts at: 1278 2081 2602 3280 3554 3701 4112 1278
Size: 803 521 678 274 147 411 2058
Fragments arranged by size:
2058 803 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1183 1213 1433 1463 1183
Size: 30 220 30 4612
Fragments arranged by size:
4612 220 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 1378 1797 610
Size: 768 419 3705
Fragments arranged by size:
3705 768 419
BseYI C'CCAG_C
Cuts at: 626 791 1437 2317 3075 3652 4148 626
Size: 165 646 880 758 577 496 1370
Fragments arranged by size:
1370 880 758 646 577 496 165
BsiEI CG_ry'CG
Cuts at: 104 2434 2480 3688 104
Size: 2330 46 1208 1308
Fragments arranged by size:
2330 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 848 1025 1799 1906 2684 2874 3666 848
Size: 177 774 107 778 190 792 2074
Fragments arranged by size:
2074 792 778 774 190 177 107
Bsp1286I G_dGCh'C
Cuts at: 343 446 691 775 848 1025 1186 1231 1304
Size: 103 245 84 73 177 161 45 73
Cuts at: 1304 1468 1511 1650 1702 1799 1906 2517 2610
Size: 164 43 139 52 97 107 611 93
Cuts at: 2610 2684 2874 2936 3666 343
Size: 74 190 62 730 1569
Fragments arranged by size:
1569 730 611 245 190 177 164 161
139 107 103 97 93 84 74 73
73 62 52 45 43
BspEI T'CCGG_A
Cuts at: 4112 4112
Size: 4892
BspHI T'CATG_A
Cuts at: 4068 4068
Size: 4892
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 1154 1942 2156 2458 2839 442
Size: 712 788 214 302 381 2495
Fragments arranged by size:
2495 788 712 381 302 214
BsrBI CCG'CTC
Cuts at: 50 155 467 788 3184 3238 4722 4887 50
Size: 105 312 321 2396 54 1484 165 55
Fragments arranged by size:
2396 1484 321 312 165 105 55 54
BsrDI GCAATG_nn'
Cuts at: 1874 2804 1874
Size: 930 3962
BsrFI r'CCGG_y
Cuts at: 1481 1574 2890 3071 3280 1481
Size: 93 1316 181 209 3093
Fragments arranged by size:
3093 1316 209 181 93
BsrGI T'GTAC_A
Cuts at: 1398 1398
Size: 4892
BssSI C'ACGA_G
Cuts at: 3163 3521 3163
Size: 358 4534
BstAPI GCAn_nnn'nTGC
Cuts at: 2269 2269
Size: 4892
BstBI TT'CG_AA
Cuts at: 3253 3253
Size: 4892
BstEII G'GTnAC_C
Cuts at: 1764 1764
Size: 4892
BstXI CCAn_nnnn'nTGG
Cuts at: 1485 1485
Size: 4892
BstYI r'GATC_y
Cuts at: 1 1091 1316 1917 2384 2407 2742 2988 4000
Size: 1090 225 601 467 23 335 246 1012
Cuts at: 4000 4086 4098 4444 1
Size: 86 12 346 449
Fragments arranged by size:
1090 1012 601 467 449 346 335 246
225 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1027 1027
Size: 4892
BtgI C'CryG_G
Cuts at: 107 344 860 1058 1223 1565 1619 107
Size: 237 516 198 165 342 54 3380
Fragments arranged by size:
3380 516 342 237 198 165 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2842 3023 3186 2842
Size: 181 163 4548
Fragments arranged by size:
4548 181 163
BtsI GCAGTG_nn'
Cuts at: 2135 2135
Size: 4892
DraI TTT'AAA
Cuts at: 1891 1891
Size: 4892
DraIII CAC_nnn'GTG
Cuts at: 644 644
Size: 4892
DrdI GACnn_nn'nnGTC
Cuts at: 2598 3456 2598
Size: 858 4034
EaeI y'GGCC_r
Cuts at: 668 725 1220 1373 1622 1733 2477 2651 3042
Size: 57 495 153 249 111 744 174 391
Cuts at: 3042 3069 4414 668
Size: 27 1345 1146
Fragments arranged by size:
1345 1146 744 495 391 249 174 153
111 57 27
EagI C'GGCC_G
Cuts at: 2477 2477
Size: 4892
EarI CTCTTCn'nnn_
Cuts at: 2915 3125 2915
Size: 210 4682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3100 3410 3556 4702 3100
Size: 310 146 1146 3290
Fragments arranged by size:
3290 1146 310 146
EcoICRI GAG'CTC
Cuts at: 846 1023 1904 846
Size: 177 881 3834
Fragments arranged by size:
3834 881 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 340 1450 1461 1507 1867 1888 2717 3149
Size: 195 1110 11 46 360 21 829 432
Cuts at: 3149 3896 4164 145
Size: 747 268 873
Fragments arranged by size:
1110 873 829 747 432 360 268 195
46 21 11
EcoNI CCTnn'n_nnAGG
Cuts at: 262 262
Size: 4892
EcoO109I rG'GnC_Cy
Cuts at: 363 386 687 771 1031 1215 1323 1472 1699
Size: 23 301 84 260 184 108 149 227
Cuts at: 1699 2031 363
Size: 332 3224
Fragments arranged by size:
3224 332 301 260 227 184 149 108
84 23
EcoRI G'AATT_C
Cuts at: 1896 1896
Size: 4892
EcoRV GAT'ATC
Cuts at: 255 255
Size: 4892
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 960 2911 960
Size: 1951 2941
FspI TGC'GCA
Cuts at: 223 2673 223
Size: 2450 2442
FspAI rTGC'GCAy
Cuts at: 223 223
Size: 4892
HaeII r_GCGC'y
Cuts at: 115 2326 2574 3596 4575 115
Size: 2211 248 1022 979 432
Fragments arranged by size:
2211 1022 979 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 811 843 1433 1465 2734 2764 2766 2796 811
Size: 32 590 32 1269 30 2 30 2907
Fragments arranged by size:
2907 1269 590 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1931 1931
Size: 4892
KasI G'GCGC_C
Cuts at: 111 2322 2570 111
Size: 2211 248 2433
Fragments arranged by size:
2433 2211 248
KpnI G_GTAC'C
Cuts at: 1912 1912
Size: 4892
MluI A'CGCG_T
Cuts at: 4325 4325
Size: 4892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1631 3563 3747 1256
Size: 375 1932 184 2401
Fragments arranged by size:
2401 1932 375 184
MscI TGG'CCA
Cuts at: 727 1222 1375 1735 2653 727
Size: 495 153 360 918 2966
Fragments arranged by size:
2966 918 495 360 153
MslI CAynn'nnrTG
Cuts at: 1394 2881 3008 1394
Size: 1487 127 3278
Fragments arranged by size:
3278 1487 127
MspA1I CmG'CkG
Cuts at: 795 817 1437 1521 1529 1639 2003 2317 2677
Size: 22 620 84 8 110 364 314 360
Cuts at: 2677 3690 3935 795
Size: 1013 245 1752
Fragments arranged by size:
1752 1013 620 364 360 314 245 110
84 22 8
NaeI GCC'GGC
Cuts at: 1483 3073 1483
Size: 1590 3302
NarI GG'CG_CC
Cuts at: 112 2323 2571 112
Size: 2211 248 2433
Fragments arranged by size:
2433 2211 248
NcoI C'CATG_G
Cuts at: 107 860 1223 107
Size: 753 363 3776
Fragments arranged by size:
3776 753 363
NgoMIV G'CCGG_C
Cuts at: 1481 3071 1481
Size: 1590 3302
NsiI A_TGCA'T
Cuts at: 992 992
Size: 4892
NspI r_CATG'y
Cuts at: 222 990 1393 1945 2445 3352 222
Size: 768 403 552 500 907 1762
Fragments arranged by size:
1762 907 768 552 500 403
PciI A'CATG_T
Cuts at: 3348 3348
Size: 4892
PflMI CCAn_nnn'nTGG
Cuts at: 861 861
Size: 4892
PfoI T'CCnGG_A
Cuts at: 1835 1835
Size: 4892
PmeI GTTT'AAAC
Cuts at: 1891 1891
Size: 4892
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 433 465 835 867 4031 4063 433
Size: 32 370 32 3164 32 1262
Fragments arranged by size:
3164 1262 370 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 386 1031 1215 1323 1472 386
Size: 645 184 108 149 3806
Fragments arranged by size:
3806 645 184 149 108
PshAI GACnn'nnGTC
Cuts at: 1537 1537
Size: 4892
PspOMI G'GGCC_C
Cuts at: 687 771 1698 687
Size: 84 927 3881
Fragments arranged by size:
3881 927 84
PspXI vC'TCGA_Gb
Cuts at: 613 613
Size: 4892
PstI C_TGCA'G
Cuts at: 659 1939 2624 659
Size: 1280 685 2927
Fragments arranged by size:
2927 1280 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4892
PvuII CAG'CTG
Cuts at: 795 817 1437 1521 2317 2677 795
Size: 22 620 84 796 360 3010
Fragments arranged by size:
3010 796 620 360 84 22
RsrII CG'GwC_CG
Cuts at: 3087 3087
Size: 4892
SacI G_AGCT'C
Cuts at: 848 1025 1906 848
Size: 177 881 3834
Fragments arranged by size:
3834 881 177
SalI G'TCGA_C
Cuts at: 1929 1929
Size: 4892
SanDI GG'GwC_CC
Cuts at: 386 386
Size: 4892
SapI GCTCTTCn'nnn_
Cuts at: 2915 3125 2915
Size: 210 4682
SbfI CC_TGCA'GG
Cuts at: 659 1939 659
Size: 1280 3612
ScaI AGT'ACT
Cuts at: 4409 4409
Size: 4892
SfcI C'TryA_G
Cuts at: 33 655 1935 2620 3613 3804 33
Size: 622 1280 685 993 191 1121
Fragments arranged by size:
1280 1121 993 685 622 191
SfoI GGC'GCC
Cuts at: 113 2324 2572 113
Size: 2211 248 2433
Fragments arranged by size:
2433 2211 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4892
SmaI CCC'GGG
Cuts at: 1461 1693 1914 1461
Size: 232 221 4439
Fragments arranged by size:
4439 232 221
SmlI C'TyrA_G
Cuts at: 613 2047 2962 3454 3716 613
Size: 1434 915 492 262 1789
Fragments arranged by size:
1789 1434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4892
SphI G_CATG'C
Cuts at: 222 1393 1945 2445 222
Size: 1171 552 500 2669
Fragments arranged by size:
2669 1171 552 500
SrfI GCCC'GGGC
Cuts at: 1693 1693
Size: 4892
StyI C'CwwG_G
Cuts at: 107 860 1223 2026 2380 107
Size: 753 363 803 354 2619
Fragments arranged by size:
2619 803 753 363 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 227 960 1645 2475 2608 3067 3276 227
Size: 733 685 830 133 459 209 1843
Fragments arranged by size:
1843 830 733 685 459 209 133
TatI w'GTAC_w
Cuts at: 1398 4407 1398
Size: 3009 1883
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 172 204 1023 1055 1183 1215 4409 4441 172
Size: 32 819 32 128 32 3194 32 623
Fragments arranged by size:
3194 819 623 128 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2689 2689
Size: 4892
XbaI T'CTAG_A
Cuts at: 1923 1923
Size: 4892
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 315 2387 315
Size: 2072 2820
XhoI C'TCGA_G
Cuts at: 613 613
Size: 4892
XmaI C'CCGG_G
Cuts at: 1459 1691 1912 1459
Size: 232 221 4439
Fragments arranged by size:
4439 232 221
XmnI GAAnn'nnTTC
Cuts at: 146 308 739 1174 146
Size: 162 431 435 3864
Fragments arranged by size:
3864 435 431 162
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BcgI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BssHII BstZ17I ClaI CspCI FseI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PmlI PsiI
PsrI SacII SexAI SfiI SgrAI SnaBI SspI StuI
SwaI ZraI