Restriction map for
pF1KB6151
(Circular) (Six-base) MAPSORT of: pF1KB6151.seq Check: 8095 from: 1 to: 4901
pF1KB6151 4901 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 1939 1939
Size: 4901
Acc65I G'GTAC_C
Cuts at: 1917 1917
Size: 4901
AclI AA'CG_TT
Cuts at: 4741 4741
Size: 4901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 2726 3158 3905 4173 1229
Size: 1497 432 747 268 1957
Fragments arranged by size:
1957 1497 747 432 268
AflIII A'CryG_T
Cuts at: 146 2253 3357 4334 146
Size: 2107 1104 977 713
Fragments arranged by size:
2107 1104 977 713
AgeI A'CCGG_T
Cuts at: 3289 3289
Size: 4901
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 280 312 280
Size: 32 4869
AlwNI CAG_nnn'CTG
Cuts at: 1692 2206 3773 1692
Size: 514 1567 2820
Fragments arranged by size:
2820 1567 514
ApaI G_GGCC'C
Cuts at: 1670 1670
Size: 4901
ApaLI G'TGCA_C
Cuts at: 3671 3671
Size: 4901
ApoI r'AATT_y
Cuts at: 759 863 907 975 1905 4254 4510 759
Size: 104 44 68 930 2349 256 1150
Fragments arranged by size:
2349 1150 930 256 104 68 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4901
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
AvaI C'yCGr_G
Cuts at: 1921 1921
Size: 4901
BamHI G'GATC_C
Cuts at: 502 1926 502
Size: 1424 3477
BanI G'GyrC_C
Cuts at: 1917 2331 2579 2614 1917
Size: 414 248 35 4204
Fragments arranged by size:
4204 414 248 35
BanII G_rGCy'C
Cuts at: 992 1156 1367 1670 1915 2945 992
Size: 164 211 303 245 1030 2948
Fragments arranged by size:
2948 1030 303 245 211 164
BbeI G_GCGC'C
Cuts at: 2335 2583 2335
Size: 248 4653
BbsI GAAGACnn'nnnn_
Cuts at: 1217 1217
Size: 4901
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1136 1170 1136
Size: 34 4867
BciVI GTATCCnnnnn_n'
Cuts at: 2799 3560 4901 2799
Size: 761 1341 2799
Fragments arranged by size:
2799 1341 761
BclI T'GATC_A
Cuts at: 725 1187 1316 2421 725
Size: 462 129 1105 3205
Fragments arranged by size:
3205 1105 462 129
BglI GCCn_nnn'nGGC
Cuts at: 112 1629 112
Size: 1517 3384
BglII A'GATC_T
Cuts at: 534 680 2416 4453 534
Size: 146 1736 2037 982
Fragments arranged by size:
2037 1736 982 146
BlpI GC'TnA_GC
Cuts at: 1039 2013 1039
Size: 974 3927
Bme1580I G_kGCm'C
Cuts at: 1670 2526 2619 3675 1670
Size: 856 93 1056 2896
Fragments arranged by size:
2896 1056 856 93
BmrI ACTGGGnnnn_n'
Cuts at: 1119 1797 2113 2194 2529 4673 1119
Size: 678 316 81 335 2144 1347
Fragments arranged by size:
2144 1347 678 335 316 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1420 3268 1420
Size: 1848 3053
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 884 1390 1468 622
Size: 262 506 78 4055
Fragments arranged by size:
4055 506 262 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 2956 3448 3746 2077
Size: 879 492 298 3232
Fragments arranged by size:
3232 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4605 4605
Size: 4901
BsaAI yAC'GTr
Cuts at: 2254 2254
Size: 4901
BsaBI GATnn'nnATC
Cuts at: 6 554 2415 4452 6
Size: 548 1861 2037 455
Fragments arranged by size:
2037 1861 548 455
BsaHI Gr'CG_yC
Cuts at: 2332 2580 3282 4778 2332
Size: 248 702 1496 2455
Fragments arranged by size:
2455 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2090 2611 3289 3563 3710 4121 2090
Size: 521 678 274 147 411 2870
Fragments arranged by size:
2870 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1234 1264 1234
Size: 30 4871
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 535 1000 1657 1693 535
Size: 465 657 36 3743
Fragments arranged by size:
3743 657 465 36
BseYI C'CCAG_C
Cuts at: 1607 2326 3084 3661 4157 1607
Size: 719 758 577 496 2351
Fragments arranged by size:
2351 758 719 577 496
BsiEI CG_ry'CG
Cuts at: 104 132 2443 2489 3697 104
Size: 28 2311 46 1208 1308
Fragments arranged by size:
2311 1308 1208 46 28
BsiHKAI G_wGCw'C
Cuts at: 401 992 1040 1427 1915 2693 2883 3675 401
Size: 591 48 387 488 778 190 792 1627
Fragments arranged by size:
1627 792 778 591 488 387 190 48
BsmI GAATG_Cn'
Cuts at: 583 703 872 1069 583
Size: 120 169 197 4415
Fragments arranged by size:
4415 197 169 120
Bsp1286I G_dGCh'C
Cuts at: 401 992 1040 1156 1367 1427 1670 1915 2526
Size: 591 48 116 211 60 243 245 611
Cuts at: 2526 2619 2693 2883 2945 3675 401
Size: 93 74 190 62 730 1627
Fragments arranged by size:
1627 730 611 591 245 243 211 190
116 93 74 62 60 48
BspEI T'CCGG_A
Cuts at: 4121 4121
Size: 4901
BspHI T'CATG_A
Cuts at: 4077 4077
Size: 4901
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1951 2165 2467 2848 1951
Size: 214 302 381 4004
Fragments arranged by size:
4004 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1590 1683 3193 3247 4731 4896 50
Size: 1540 93 1510 54 1484 165 55
Fragments arranged by size:
1540 1510 1484 165 93 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 2813 1832
Size: 981 3920
BsrFI r'CCGG_y
Cuts at: 321 1456 2899 3080 3289 321
Size: 1135 1443 181 209 1933
Fragments arranged by size:
1933 1443 1135 209 181
BsrGI T'GTAC_A
Cuts at: 1571 1571
Size: 4901
BssSI C'ACGA_G
Cuts at: 3172 3530 3172
Size: 358 4543
BstAPI GCAn_nnn'nTGC
Cuts at: 1692 2278 1692
Size: 586 4315
BstBI TT'CG_AA
Cuts at: 3262 3262
Size: 4901
BstEII G'GTnAC_C
Cuts at: 607 607
Size: 4901
BstXI CCAn_nnnn'nTGG
Cuts at: 685 685
Size: 4901
BstYI r'GATC_y
Cuts at: 1 486 502 534 555 680 1001 1926 2393
Size: 485 16 32 21 125 321 925 467
Cuts at: 2393 2416 2751 2997 4009 4095 4107 4453 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 925 485 467 449 346 335 321
246 125 86 32 23 21 16 12
BtgI C'CryG_G
Cuts at: 107 566 950 1097 1232 1553 107
Size: 459 384 147 135 321 3455
Fragments arranged by size:
3455 459 384 321 147 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 944 2851 3032 3195 944
Size: 1907 181 163 2650
Fragments arranged by size:
2650 1907 181 163
BtsI GCAGTG_nn'
Cuts at: 1690 2144 1690
Size: 454 4447
DraI TTT'AAA
Cuts at: 1900 1900
Size: 4901
DrdI GACnn_nn'nnGTC
Cuts at: 2607 3465 2607
Size: 858 4043
EaeI y'GGCC_r
Cuts at: 129 713 2486 2660 3051 3078 4423 129
Size: 584 1773 174 391 27 1345 607
Fragments arranged by size:
1773 1345 607 584 391 174 27
EagI C'GGCC_G
Cuts at: 129 2486 129
Size: 2357 2544
EarI CTCTTCn'nnn_
Cuts at: 730 2924 3134 730
Size: 2194 210 2497
Fragments arranged by size:
2497 2194 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1647 3109 3419 3565 4711 1647
Size: 1462 310 146 1146 1837
Fragments arranged by size:
1837 1462 1146 310 146
EcoICRI GAG'CTC
Cuts at: 990 1913 990
Size: 923 3978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 622 884 1229 1390 1468 2726 3158 3905 4173
Size: 262 345 161 78 1258 432 747 268
Cuts at: 4173 622
Size: 1350
Fragments arranged by size:
1350 1258 747 432 345 268 262 161
78
EcoNI CCTnn'n_nnAGG
Cuts at: 1450 1450
Size: 4901
EcoO109I rG'GnC_Cy
Cuts at: 942 1647 1666 1667 2040 942
Size: 705 19 1 373 3803
Fragments arranged by size:
3803 705 373 19 1
EcoRI G'AATT_C
Cuts at: 759 975 1905 759
Size: 216 930 3755
Fragments arranged by size:
3755 930 216
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4901
FseI GG_CCGG'CC
Cuts at: 1460 1460
Size: 4901
FspI TGC'GCA
Cuts at: 198 2682 198
Size: 2484 2417
HaeII r_GCGC'y
Cuts at: 2335 2583 3605 4584 2335
Size: 248 1022 979 2652
Fragments arranged by size:
2652 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 547 579 1234 1266 1860 1892 2743 2773 2775
Size: 32 655 32 594 32 851 30 2
Cuts at: 2775 2805 547
Size: 30 2643
Fragments arranged by size:
2643 851 655 594 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1275 1720 1940 1275
Size: 445 220 4236
Fragments arranged by size:
4236 445 220
KasI G'GCGC_C
Cuts at: 2331 2579 2331
Size: 248 4653
KpnI G_GTAC'C
Cuts at: 1921 1921
Size: 4901
MfeI C'AATT_G
Cuts at: 1175 1175
Size: 4901
MluI A'CGCG_T
Cuts at: 4334 4334
Size: 4901
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1787 1846 3572 3756 1787
Size: 59 1726 184 2932
Fragments arranged by size:
2932 1726 184 59
MscI TGG'CCA
Cuts at: 715 2662 715
Size: 1947 2954
MslI CAynn'nnrTG
Cuts at: 2890 3017 2890
Size: 127 4774
MspA1I CmG'CkG
Cuts at: 328 477 837 972 1611 2012 2326 2686 3699
Size: 149 360 135 639 401 314 360 1013
Cuts at: 3699 3944 328
Size: 245 1285
Fragments arranged by size:
1285 1013 639 401 360 360 314 245
149 135
NaeI GCC'GGC
Cuts at: 323 1458 3082 323
Size: 1135 1624 2142
Fragments arranged by size:
2142 1624 1135
NarI GG'CG_CC
Cuts at: 2332 2580 2332
Size: 248 4653
NcoI C'CATG_G
Cuts at: 107 566 107
Size: 459 4442
NdeI CA'TA_TG
Cuts at: 843 843
Size: 4901
NgoMIV G'CCGG_C
Cuts at: 321 1456 3080 321
Size: 1135 1624 2142
Fragments arranged by size:
2142 1624 1135
NruI TCG'CGA
Cuts at: 170 170
Size: 4901
NspI r_CATG'y
Cuts at: 1140 1954 2454 3361 1140
Size: 814 500 907 2680
Fragments arranged by size:
2680 907 814 500
PasI CC'CwG_GG
Cuts at: 1663 1663
Size: 4901
PciI A'CATG_T
Cuts at: 3357 3357
Size: 4901
PflMI CCAn_nnn'nTGG
Cuts at: 550 550
Size: 4901
PfoI T'CCnGG_A
Cuts at: 185 185
Size: 4901
PmeI GTTT'AAAC
Cuts at: 1900 1900
Size: 4901
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 4040
Size: 32 4869
PpuMI rG'GwC_Cy
Cuts at: 942 1647 942
Size: 705 4196
PspOMI G'GGCC_C
Cuts at: 1666 1666
Size: 4901
PstI C_TGCA'G
Cuts at: 971 984 1068 1709 1948 2633 971
Size: 13 84 641 239 685 3239
Fragments arranged by size:
3239 685 641 239 84 13
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4901
PvuII CAG'CTG
Cuts at: 328 837 972 1611 2326 2686 328
Size: 509 135 639 715 360 2543
Fragments arranged by size:
2543 715 639 509 360 135
RsrII CG'GwC_CG
Cuts at: 3096 3096
Size: 4901
SacI G_AGCT'C
Cuts at: 992 1915 992
Size: 923 3978
SalI G'TCGA_C
Cuts at: 1938 1938
Size: 4901
SapI GCTCTTCn'nnn_
Cuts at: 2924 3134 2924
Size: 210 4691
SbfI CC_TGCA'GG
Cuts at: 1948 1948
Size: 4901
ScaI AGT'ACT
Cuts at: 4418 4418
Size: 4901
SfcI C'TryA_G
Cuts at: 33 967 980 1064 1705 1944 2629 3622 3813
Size: 934 13 84 641 239 685 993 191
Cuts at: 3813 33
Size: 1121
Fragments arranged by size:
1121 993 934 685 641 239 191 84
13
SfoI GGC'GCC
Cuts at: 2333 2581 2333
Size: 248 4653
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4901
SmaI CCC'GGG
Cuts at: 1923 1923
Size: 4901
SmlI C'TyrA_G
Cuts at: 2056 2971 3463 3725 2056
Size: 915 492 262 3232
Fragments arranged by size:
3232 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1332 67
Size: 1265 3636
SphI G_CATG'C
Cuts at: 1954 2454 1954
Size: 500 4401
SspI AAT'ATT
Cuts at: 767 767
Size: 4901
StyI C'CwwG_G
Cuts at: 107 566 635 1755 2035 2389 107
Size: 459 69 1120 280 354 2619
Fragments arranged by size:
2619 1120 459 354 280 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 481 2484 2617 3076 3285 481
Size: 2003 133 459 209 2097
Fragments arranged by size:
2097 2003 459 209 133
TatI w'GTAC_w
Cuts at: 524 1571 4416 524
Size: 1047 2845 1009
Fragments arranged by size:
2845 1047 1009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4418 4450 4418
Size: 32 4869
Tth111I GACn'n_nGTC
Cuts at: 2698 2698
Size: 4901
XbaI T'CTAG_A
Cuts at: 1932 1932
Size: 4901
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1810 2396 1810
Size: 586 4315
XmaI C'CCGG_G
Cuts at: 1921 1921
Size: 4901
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NruI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FspAI HindIII HpaI NheI NotI NsiI PacI
PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI