Restriction map for
pF1KB6154
(Circular) (Six-base) MAPSORT of: pF1KB6154.seq Check: 3868 from: 1 to: 5039
pF1KB6154 5039 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1736 1736
Size: 5039
AccI GT'mk_AC
Cuts at: 2077 2077
Size: 5039
Acc65I G'GTAC_C
Cuts at: 2055 2055
Size: 5039
AclI AA'CG_TT
Cuts at: 4879 4879
Size: 5039
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 773 889 1024 1532 1844 2048 2864 3296
Size: 268 116 135 508 312 204 816 432
Cuts at: 3296 4043 4311 505
Size: 747 268 1233
Fragments arranged by size:
1233 816 747 508 432 312 268 268
204 135 116
AflII C'TTAA_G
Cuts at: 1489 1489
Size: 5039
AflIII A'CryG_T
Cuts at: 1881 2391 3495 4472 1881
Size: 510 1104 977 2448
Fragments arranged by size:
2448 1104 977 510
AgeI A'CCGG_T
Cuts at: 3427 3427
Size: 5039
AlwNI CAG_nnn'CTG
Cuts at: 2344 3911 2344
Size: 1567 3472
ApaLI G'TGCA_C
Cuts at: 3809 3809
Size: 5039
ApoI r'AATT_y
Cuts at: 1398 2043 4392 4648 1398
Size: 645 2349 256 1789
Fragments arranged by size:
2349 1789 645 256
AseI AT'TA_AT
Cuts at: 20 1247 1523 20
Size: 1227 276 3536
Fragments arranged by size:
3536 1227 276
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
AvaI C'yCGr_G
Cuts at: 354 1435 2059 354
Size: 1081 624 3334
Fragments arranged by size:
3334 1081 624
BamHI G'GATC_C
Cuts at: 301 2064 301
Size: 1763 3276
BanI G'GyrC_C
Cuts at: 823 1444 2055 2469 2717 2752 823
Size: 621 611 414 248 35 3110
Fragments arranged by size:
3110 621 611 414 248 35
BanII G_rGCy'C
Cuts at: 630 1473 1757 2053 3083 630
Size: 843 284 296 1030 2586
Fragments arranged by size:
2586 1030 843 296 284
BbeI G_GCGC'C
Cuts at: 2473 2721 2473
Size: 248 4791
BbsI GAAGACnn'nnnn_
Cuts at: 2015 2015
Size: 5039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 376 410 1426 1460 376
Size: 34 1016 34 3955
Fragments arranged by size:
3955 1016 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 596 1551 2937 3698 5039 596
Size: 955 1386 761 1341 596
Fragments arranged by size:
1386 1341 955 761 596
BclI T'GATC_A
Cuts at: 2559 2559
Size: 5039
BfrBI ATG'CAT
Cuts at: 768 1949 768
Size: 1181 3858
BglII A'GATC_T
Cuts at: 488 1959 2554 4591 488
Size: 1471 595 2037 936
Fragments arranged by size:
2037 1471 936 595
BlpI GC'TnA_GC
Cuts at: 2151 2151
Size: 5039
Bme1580I G_kGCm'C
Cuts at: 520 2664 2757 3813 520
Size: 2144 93 1056 1746
Fragments arranged by size:
2144 1746 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2251 2332 2667 4811 2251
Size: 81 335 2144 2479
Fragments arranged by size:
2479 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3406 3406
Size: 5039
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1457 1723 1927 2215 3094 3586 3884 1457
Size: 266 204 288 879 492 298 2612
Fragments arranged by size:
2612 879 492 298 288 266 204
BsaI GGTCTCn'nnnn_
Cuts at: 351 1102 4743 351
Size: 751 3641 647
Fragments arranged by size:
3641 751 647
BsaAI yAC'GTr
Cuts at: 2392 2392
Size: 5039
BsaBI GATnn'nnATC
Cuts at: 6 2553 4590 6
Size: 2547 2037 455
Fragments arranged by size:
2547 2037 455
BsaHI Gr'CG_yC
Cuts at: 2470 2718 3420 4916 2470
Size: 248 702 1496 2593
Fragments arranged by size:
2593 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2228 2749 3427 3701 3848 4259 2228
Size: 521 678 274 147 411 3008
Fragments arranged by size:
3008 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 620 1312 1924 620
Size: 692 612 3735
Fragments arranged by size:
3735 692 612
BseYI C'CCAG_C
Cuts at: 2464 3222 3799 4295 2464
Size: 758 577 496 3208
Fragments arranged by size:
3208 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2581 2627 3835 104
Size: 2477 46 1208 1308
Fragments arranged by size:
2477 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2053 2831 3021 3813 2053
Size: 778 190 792 3279
Fragments arranged by size:
3279 792 778 190
BsmI GAATG_Cn'
Cuts at: 376 852 1581 1951 376
Size: 476 729 370 3464
Fragments arranged by size:
3464 729 476 370
Bsp1286I G_dGCh'C
Cuts at: 520 630 1473 1757 2053 2664 2757 2831 3021
Size: 110 843 284 296 611 93 74 190
Cuts at: 3021 3083 3813 520
Size: 62 730 1746
Fragments arranged by size:
1746 843 730 611 296 284 190 110
93 74 62
BspEI T'CCGG_A
Cuts at: 4259 4259
Size: 5039
BspHI T'CATG_A
Cuts at: 1218 4215 1218
Size: 2997 2042
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1736 2089 2303 2605 2986 1736
Size: 353 214 302 381 3789
Fragments arranged by size:
3789 381 353 302 214
BsrBI CCG'CTC
Cuts at: 50 3331 3385 4869 5034 50
Size: 3281 54 1484 165 55
Fragments arranged by size:
3281 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1586 2951 1586
Size: 1365 3674
BsrFI r'CCGG_y
Cuts at: 3037 3218 3427 3037
Size: 181 209 4649
Fragments arranged by size:
4649 209 181
BsrGI T'GTAC_A
Cuts at: 614 614
Size: 5039
BssSI C'ACGA_G
Cuts at: 3310 3668 3310
Size: 358 4681
BstAPI GCAn_nnn'nTGC
Cuts at: 2416 2416
Size: 5039
BstBI TT'CG_AA
Cuts at: 3400 3400
Size: 5039
BstXI CCAn_nnnn'nTGG
Cuts at: 1266 1266
Size: 5039
BstYI r'GATC_y
Cuts at: 1 166 301 488 1959 2064 2531 2554 2889
Size: 165 135 187 1471 105 467 23 335
Cuts at: 2889 3135 4147 4233 4245 4591 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1471 1012 467 449 346 335 246 187
165 135 105 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2989 3170 3333 2989
Size: 181 163 4695
Fragments arranged by size:
4695 181 163
BtsI GCAGTG_nn'
Cuts at: 394 2282 394
Size: 1888 3151
DraI TTT'AAA
Cuts at: 573 2038 573
Size: 1465 3574
DraIII CAC_nnn'GTG
Cuts at: 651 651
Size: 5039
DrdI GACnn_nn'nnGTC
Cuts at: 2745 3603 2745
Size: 858 4181
EaeI y'GGCC_r
Cuts at: 141 2624 2798 3189 3216 4561 141
Size: 2483 174 391 27 1345 619
Fragments arranged by size:
2483 1345 619 391 174 27
EagI C'GGCC_G
Cuts at: 2624 2624
Size: 5039
EarI CTCTTCn'nnn_
Cuts at: 548 1670 3062 3272 548
Size: 1122 1392 210 2315
Fragments arranged by size:
2315 1392 1122 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3247 3557 3703 4849 3247
Size: 310 146 1146 3437
Fragments arranged by size:
3437 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 773 889 1024 1532 1844 2048 2864 3296
Size: 268 116 135 508 312 204 816 432
Cuts at: 3296 4043 4311 505
Size: 747 268 1233
Fragments arranged by size:
1233 816 747 508 432 312 268 268
204 135 116
EcoO109I rG'GnC_Cy
Cuts at: 351 1263 2178 351
Size: 912 915 3212
Fragments arranged by size:
3212 915 912
EcoRI G'AATT_C
Cuts at: 1398 2043 1398
Size: 645 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 731 1029 3058 731
Size: 298 2029 2712
Fragments arranged by size:
2712 2029 298
FspI TGC'GCA
Cuts at: 2820 2820
Size: 5039
HaeII r_GCGC'y
Cuts at: 2473 2721 3743 4722 2473
Size: 248 1022 979 2790
Fragments arranged by size:
2790 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 151 183 844 876 1690 1722 2881 2911 2913
Size: 32 661 32 814 32 1159 30 2
Cuts at: 2913 2943 151
Size: 30 2247
Fragments arranged by size:
2247 1159 814 661 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 316 1746 2078 316
Size: 1430 332 3277
Fragments arranged by size:
3277 1430 332
HpaI GTT'AAC
Cuts at: 1746 1746
Size: 5039
KasI G'GCGC_C
Cuts at: 2469 2717 2469
Size: 248 4791
KpnI G_GTAC'C
Cuts at: 2059 2059
Size: 5039
MfeI C'AATT_G
Cuts at: 1570 1570
Size: 5039
MluI A'CGCG_T
Cuts at: 4472 4472
Size: 5039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3710 3894 3710
Size: 184 4855
MscI TGG'CCA
Cuts at: 2800 2800
Size: 5039
MslI CAynn'nnrTG
Cuts at: 3028 3155 3028
Size: 127 4912
MspA1I CmG'CkG
Cuts at: 131 1791 2150 2464 2824 3837 4082 131
Size: 1660 359 314 360 1013 245 1088
Fragments arranged by size:
1660 1088 1013 360 359 314 245
NaeI GCC'GGC
Cuts at: 3220 3220
Size: 5039
NarI GG'CG_CC
Cuts at: 2470 2718 2470
Size: 248 4791
NgoMIV G'CCGG_C
Cuts at: 3218 3218
Size: 5039
NsiI A_TGCA'T
Cuts at: 770 1951 770
Size: 1181 3858
NspI r_CATG'y
Cuts at: 1885 1953 2092 2592 3499 1885
Size: 68 139 500 907 3425
Fragments arranged by size:
3425 907 500 139 68
PciI A'CATG_T
Cuts at: 1881 3495 1881
Size: 1614 3425
PflMI CCAn_nnn'nTGG
Cuts at: 226 226
Size: 5039
PfoI T'CCnGG_A
Cuts at: 304 304
Size: 5039
PmeI GTTT'AAAC
Cuts at: 2038 2038
Size: 5039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 5007
PpuMI rG'GwC_Cy
Cuts at: 351 1263 351
Size: 912 4127
PstI C_TGCA'G
Cuts at: 860 2086 2771 860
Size: 1226 685 3128
Fragments arranged by size:
3128 1226 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5039
PvuII CAG'CTG
Cuts at: 2464 2824 2464
Size: 360 4679
RsrII CG'GwC_CG
Cuts at: 3234 3234
Size: 5039
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5039
SalI G'TCGA_C
Cuts at: 2076 2076
Size: 5039
SapI GCTCTTCn'nnn_
Cuts at: 3062 3272 3062
Size: 210 4829
SbfI CC_TGCA'GG
Cuts at: 2086 2086
Size: 5039
ScaI AGT'ACT
Cuts at: 1189 1740 4556 1189
Size: 551 2816 1672
Fragments arranged by size:
2816 1672 551
SfcI C'TryA_G
Cuts at: 33 856 1162 1892 2082 2767 3760 3951 33
Size: 823 306 730 190 685 993 191 1121
Fragments arranged by size:
1121 993 823 730 685 306 191 190
SfoI GGC'GCC
Cuts at: 2471 2719 2471
Size: 248 4791
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5039
SmaI CCC'GGG
Cuts at: 2061 2061
Size: 5039
SmlI C'TyrA_G
Cuts at: 1472 1489 1702 1906 2194 3109 3601 3863 1472
Size: 17 213 204 288 915 492 262 2648
Fragments arranged by size:
2648 915 492 288 262 213 204 17
SpeI A'CTAG_T
Cuts at: 67 289 1803 67
Size: 222 1514 3303
Fragments arranged by size:
3303 1514 222
SphI G_CATG'C
Cuts at: 2092 2592 2092
Size: 500 4539
SspI AAT'ATT
Cuts at: 1974 1974
Size: 5039
StuI AGG'CCT
Cuts at: 191 191
Size: 5039
StyI C'CwwG_G
Cuts at: 579 736 1259 1709 2173 2527 579
Size: 157 523 450 464 354 3091
Fragments arranged by size:
3091 523 464 450 354 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2622 2755 3214 3423 2622
Size: 133 459 209 4238
Fragments arranged by size:
4238 459 209 133
TatI w'GTAC_w
Cuts at: 614 1187 1738 4554 614
Size: 573 551 2816 1099
Fragments arranged by size:
2816 1099 573 551
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4556 4588 4556
Size: 32 5007
Tth111I GACn'n_nGTC
Cuts at: 2836 2836
Size: 5039
XbaI T'CTAG_A
Cuts at: 984 2070 984
Size: 1086 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 618 1090 2534 618
Size: 472 1444 3123
Fragments arranged by size:
3123 1444 472
XmaI C'CCGG_G
Cuts at: 2059 2059
Size: 5039
XmnI GAAnn'nnTTC
Cuts at: 490 557 1625 490
Size: 67 1068 3904
Fragments arranged by size:
3904 1068 67
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BpmI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HindIII
NcoI NdeI NheI NotI NruI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI