Restriction map for
pF1KB6157
(Circular) (Six-base) MAPSORT of: pF1KB6157.seq Check: 5746 from: 1 to: 4907
pF1KB6157 4907 bp
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1794 1794
Size: 4907
AccI GT'mk_AC
Cuts at: 1284 1323 1945 1284
Size: 39 622 4246
Fragments arranged by size:
4246 622 39
Acc65I G'GTAC_C
Cuts at: 1923 1923
Size: 4907
AclI AA'CG_TT
Cuts at: 4747 4747
Size: 4907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1121 1565 2732 3164 3911 4179 706
Size: 415 444 1167 432 747 268 1434
Fragments arranged by size:
1434 1167 747 444 432 415 268
AflII C'TTAA_G
Cuts at: 979 979
Size: 4907
AflIII A'CryG_T
Cuts at: 2259 3363 4340 2259
Size: 1104 977 2826
Fragments arranged by size:
2826 1104 977
AgeI A'CCGG_T
Cuts at: 3295 3295
Size: 4907
AhdI GACnn_n'nnGTC
Cuts at: 1674 1674
Size: 4907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1900 1900
Size: 4907
AlwNI CAG_nnn'CTG
Cuts at: 129 2212 3779 129
Size: 2083 1567 1257
Fragments arranged by size:
2083 1567 1257
ApaLI G'TGCA_C
Cuts at: 1393 3677 1393
Size: 2284 2623
ApoI r'AATT_y
Cuts at: 956 1911 4260 4516 956
Size: 955 2349 256 1347
Fragments arranged by size:
2349 1347 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4907
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
AvaI C'yCGr_G
Cuts at: 922 945 1445 1927 922
Size: 23 500 482 3902
Fragments arranged by size:
3902 500 482 23
BamHI G'GATC_C
Cuts at: 1932 1932
Size: 4907
BanI G'GyrC_C
Cuts at: 1787 1813 1923 2337 2585 2620 1787
Size: 26 110 414 248 35 4074
Fragments arranged by size:
4074 414 248 110 35 26
BanII G_rGCy'C
Cuts at: 1484 1873 1921 2951 1484
Size: 389 48 1030 3440
Fragments arranged by size:
3440 1030 389 48
BbeI G_GCGC'C
Cuts at: 1791 2341 2589 1791
Size: 550 248 4109
Fragments arranged by size:
4109 550 248
BbsI GAAGACnn'nnnn_
Cuts at: 1310 1661 1310
Size: 351 4556
BbvCI CC'TCA_GC
Cuts at: 1738 1738
Size: 4907
BciVI GTATCCnnnnn_n'
Cuts at: 2805 3566 4907 2805
Size: 761 1341 2805
Fragments arranged by size:
2805 1341 761
BclI T'GATC_A
Cuts at: 2427 2427
Size: 4907
BfrBI ATG'CAT
Cuts at: 1877 1877
Size: 4907
BglI GCCn_nnn'nGGC
Cuts at: 878 878
Size: 4907
BglII A'GATC_T
Cuts at: 146 1530 2422 4459 146
Size: 1384 892 2037 594
Fragments arranged by size:
2037 1384 892 594
BlpI GC'TnA_GC
Cuts at: 633 1259 2019 633
Size: 626 760 3521
Fragments arranged by size:
3521 760 626
Bme1580I G_kGCm'C
Cuts at: 1397 2532 2625 3681 1397
Size: 1135 93 1056 2623
Fragments arranged by size:
2623 1135 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 725 2119 2200 2535 4679 725
Size: 1394 81 335 2144 953
Fragments arranged by size:
2144 1394 953 335 81
BmtI G_CTAG'C
Cuts at: 841 841
Size: 4907
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3274 3274
Size: 4907
Bpu10I CC'TnA_GC
Cuts at: 1738 1738
Size: 4907
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 784 1588 2083 2962 3454 3752 784
Size: 804 495 879 492 298 1939
Fragments arranged by size:
1939 879 804 495 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 923 4611 923
Size: 3688 1219
BsaAI yAC'GTr
Cuts at: 1175 2260 1175
Size: 1085 3822
BsaBI GATnn'nnATC
Cuts at: 6 2421 4458 6
Size: 2415 2037 455
Fragments arranged by size:
2415 2037 455
BsaHI Gr'CG_yC
Cuts at: 1788 2338 2586 3288 4784 1788
Size: 550 248 702 1496 1911
Fragments arranged by size:
1911 1496 702 550 248
BsaWI w'CCGG_w
Cuts at: 1573 2096 2617 3295 3569 3716 4127 1573
Size: 523 521 678 274 147 411 2353
Fragments arranged by size:
2353 678 523 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 602 632 602
Size: 30 4877
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 151 181 235 623 677 680 1570 1638 151
Size: 30 54 388 54 3 890 68 3420
Fragments arranged by size:
3420 890 388 68 54 54 30 3
BseYI C'CCAG_C
Cuts at: 122 843 867 1415 2332 3090 3667 4163 122
Size: 721 24 548 917 758 577 496 866
Fragments arranged by size:
917 866 758 721 577 548 496 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1906 237
Size: 1669 3238
BsiEI CG_ry'CG
Cuts at: 104 2449 2495 3703 104
Size: 2345 46 1208 1308
Fragments arranged by size:
2345 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 954 1397 1921 2699 2889 3681 954
Size: 443 524 778 190 792 2180
Fragments arranged by size:
2180 792 778 524 443 190
Bsp1286I G_dGCh'C
Cuts at: 954 1397 1484 1873 1921 2532 2625 2699 2889
Size: 443 87 389 48 611 93 74 190
Cuts at: 2889 2951 3681 954
Size: 62 730 2180
Fragments arranged by size:
2180 730 611 443 389 190 93 87
74 62 48
BspEI T'CCGG_A
Cuts at: 1573 4127 1573
Size: 2554 2353
BspHI T'CATG_A
Cuts at: 4083 4083
Size: 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 358 1794 1878 1957 2171 2473 2854 358
Size: 1436 84 79 214 302 381 2411
Fragments arranged by size:
2411 1436 381 302 214 84 79
BsrBI CCG'CTC
Cuts at: 50 3199 3253 4737 4902 50
Size: 3149 54 1484 165 55
Fragments arranged by size:
3149 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1877 2819 1877
Size: 942 3965
BsrFI r'CCGG_y
Cuts at: 1217 1842 2905 3086 3295 1217
Size: 625 1063 181 209 2829
Fragments arranged by size:
2829 1063 625 209 181
BssSI C'ACGA_G
Cuts at: 3178 3536 3178
Size: 358 4549
BstAPI GCAn_nnn'nTGC
Cuts at: 2284 2284
Size: 4907
BstBI TT'CG_AA
Cuts at: 3268 3268
Size: 4907
BstEII G'GTnAC_C
Cuts at: 618 618
Size: 4907
BstYI r'GATC_y
Cuts at: 1 146 287 1530 1932 2399 2422 2757 3003
Size: 145 141 1243 402 467 23 335 246
Cuts at: 3003 4015 4101 4113 4459 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1243 1012 467 449 402 346 335 246
145 141 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1834 1834
Size: 4907
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2857 3038 3201 2857
Size: 181 163 4563
Fragments arranged by size:
4563 181 163
BtsI GCAGTG_nn'
Cuts at: 1324 2150 1324
Size: 826 4081
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 342 377 342
Size: 35 4872
DraI TTT'AAA
Cuts at: 1906 1906
Size: 4907
DrdI GACnn_nn'nnGTC
Cuts at: 2613 3471 2613
Size: 858 4049
EaeI y'GGCC_r
Cuts at: 363 1219 1661 2492 2666 3057 3084 4429 363
Size: 856 442 831 174 391 27 1345 841
Fragments arranged by size:
1345 856 841 831 442 391 174 27
EagI C'GGCC_G
Cuts at: 2492 2492
Size: 4907
EarI CTCTTCn'nnn_
Cuts at: 416 1097 2930 3140 416
Size: 681 1833 210 2183
Fragments arranged by size:
2183 1833 681 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 108 148 3115 3425 3571 4717 108
Size: 40 2967 310 146 1146 298
Fragments arranged by size:
2967 1146 310 298 146 40
EcoICRI GAG'CTC
Cuts at: 1919 1919
Size: 4907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 1121 1565 2732 3164 3911 4179 706
Size: 415 444 1167 432 747 268 1434
Fragments arranged by size:
1434 1167 747 444 432 415 268
EcoO109I rG'GnC_Cy
Cuts at: 2046 2046
Size: 4907
EcoRI G'AATT_C
Cuts at: 1911 1911
Size: 4907
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 292 1629 2926 292
Size: 1337 1297 2273
Fragments arranged by size:
2273 1337 1297
FspI TGC'GCA
Cuts at: 2688 2688
Size: 4907
HaeII r_GCGC'y
Cuts at: 1791 2341 2589 3611 4590 1791
Size: 550 248 1022 979 2108
Fragments arranged by size:
2108 1022 979 550 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1660 1692 2749 2779 2781 2811 1660
Size: 32 1057 30 2 30 3756
Fragments arranged by size:
3756 1057 32 30 30 2
HincII GTy'rAC
Cuts at: 521 1946 521
Size: 1425 3482
HindIII A'AGCT_T
Cuts at: 821 821
Size: 4907
KasI G'GCGC_C
Cuts at: 1787 2337 2585 1787
Size: 550 248 4109
Fragments arranged by size:
4109 550 248
KpnI G_GTAC'C
Cuts at: 1927 1927
Size: 4907
MluI A'CGCG_T
Cuts at: 4340 4340
Size: 4907
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3578 3762 3578
Size: 184 4723
MscI TGG'CCA
Cuts at: 365 1663 2668 365
Size: 1298 1005 2604
Fragments arranged by size:
2604 1298 1005
MslI CAynn'nnrTG
Cuts at: 1882 2896 3023 1882
Size: 1014 127 3766
Fragments arranged by size:
3766 1014 127
MspA1I CmG'CkG
Cuts at: 129 1698 1849 2018 2332 2692 3705 3950 129
Size: 1569 151 169 314 360 1013 245 1086
Fragments arranged by size:
1569 1086 1013 360 314 245 169 151
NaeI GCC'GGC
Cuts at: 1844 3088 1844
Size: 1244 3663
NarI GG'CG_CC
Cuts at: 1788 2338 2586 1788
Size: 550 248 4109
Fragments arranged by size:
4109 550 248
NgoMIV G'CCGG_C
Cuts at: 1842 3086 1842
Size: 1244 3663
NheI G'CTAG_C
Cuts at: 837 837
Size: 4907
NsiI A_TGCA'T
Cuts at: 1879 1879
Size: 4907
NspI r_CATG'y
Cuts at: 1960 2460 3367 1960
Size: 500 907 3500
Fragments arranged by size:
3500 907 500
PasI CC'CwG_GG
Cuts at: 1783 1783
Size: 4907
PciI A'CATG_T
Cuts at: 3363 3363
Size: 4907
PflMI CCAn_nnn'nTGG
Cuts at: 129 129
Size: 4907
PfoI T'CCnGG_A
Cuts at: 999 1250 999
Size: 251 4656
PmeI GTTT'AAAC
Cuts at: 1906 1906
Size: 4907
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4046 4078 4046
Size: 32 4875
PstI C_TGCA'G
Cuts at: 146 1489 1954 2639 146
Size: 1343 465 685 2414
Fragments arranged by size:
2414 1343 685 465
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4907
PvuII CAG'CTG
Cuts at: 129 1698 2332 2692 129
Size: 1569 634 360 2344
Fragments arranged by size:
2344 1569 634 360
RsrII CG'GwC_CG
Cuts at: 3102 3102
Size: 4907
SacI G_AGCT'C
Cuts at: 1921 1921
Size: 4907
SalI G'TCGA_C
Cuts at: 1944 1944
Size: 4907
SapI GCTCTTCn'nnn_
Cuts at: 2930 3140 2930
Size: 210 4697
SbfI CC_TGCA'GG
Cuts at: 1954 1954
Size: 4907
ScaI AGT'ACT
Cuts at: 4424 4424
Size: 4907
SfcI C'TryA_G
Cuts at: 33 142 1485 1950 2635 3628 3819 33
Size: 109 1343 465 685 993 191 1121
Fragments arranged by size:
1343 1121 993 685 465 191 109
SfoI GGC'GCC
Cuts at: 1789 2339 2587 1789
Size: 550 248 4109
Fragments arranged by size:
4109 550 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4907
SmaI CCC'GGG
Cuts at: 1929 1929
Size: 4907
SmlI C'TyrA_G
Cuts at: 799 922 979 1445 1603 2062 2977 3469 3731
Size: 123 57 466 158 459 915 492 262
Cuts at: 3731 799
Size: 1975
Fragments arranged by size:
1975 915 492 466 459 262 158 123
57
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4907
SphI G_CATG'C
Cuts at: 1960 2460 1960
Size: 500 4407
SspI AAT'ATT
Cuts at: 988 988
Size: 4907
StyI C'CwwG_G
Cuts at: 2041 2395 2041
Size: 354 4553
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2490 2623 3082 3291 2490
Size: 133 459 209 4106
Fragments arranged by size:
4106 459 209 133
TatI w'GTAC_w
Cuts at: 1464 4422 1464
Size: 2958 1949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 4875
Tth111I GACn'n_nGTC
Cuts at: 2704 2704
Size: 4907
XbaI T'CTAG_A
Cuts at: 1938 1938
Size: 4907
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1803 2402 1803
Size: 599 4308
XhoI C'TCGA_G
Cuts at: 922 1445 922
Size: 523 4384
XmaI C'CCGG_G
Cuts at: 1927 1927
Size: 4907
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AleI AloI ApaI AscI AvrII BaeI
BcgI BmgBI BpmI BsiWI BsmI BsmBI BsrGI BssHII
BstXI BstZ17I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NcoI NdeI NotI NruI PacI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XmnI ZraI