Restriction map for
pF1KB6159
(Circular) (Six-base) MAPSORT of: pF1KB6159.seq Check: 7825 from: 1 to: 4910
pF1KB6159 4910 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 975 1948 975
Size: 973 3937
Acc65I G'GTAC_C
Cuts at: 285 1926 285
Size: 1641 3269
AclI AA'CG_TT
Cuts at: 4750 4750
Size: 4910
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 478 2735 3167 3914 4182 110
Size: 368 2257 432 747 268 838
Fragments arranged by size:
2257 838 747 432 368 268
AflIII A'CryG_T
Cuts at: 486 1754 1902 2262 3366 4343 486
Size: 1268 148 360 1104 977 1053
Fragments arranged by size:
1268 1104 1053 977 360 148
AgeI A'CCGG_T
Cuts at: 3298 3298
Size: 4910
AleI CACnn'nnGTG
Cuts at: 1235 1235
Size: 4910
AlwNI CAG_nnn'CTG
Cuts at: 2215 3782 2215
Size: 1567 3343
ApaLI G'TGCA_C
Cuts at: 3680 3680
Size: 4910
ApoI r'AATT_y
Cuts at: 540 1914 4263 4519 540
Size: 1374 2349 256 931
Fragments arranged by size:
2349 1374 931 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4910
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
AvaI C'yCGr_G
Cuts at: 885 1930 885
Size: 1045 3865
BamHI G'GATC_C
Cuts at: 1935 1935
Size: 4910
BanI G'GyrC_C
Cuts at: 285 1926 2340 2588 2623 285
Size: 1641 414 248 35 2572
Fragments arranged by size:
2572 1641 414 248 35
BanII G_rGCy'C
Cuts at: 752 1924 2954 752
Size: 1172 1030 2708
Fragments arranged by size:
2708 1172 1030
BbeI G_GCGC'C
Cuts at: 2344 2592 2344
Size: 248 4662
BbvCI CC'TCA_GC
Cuts at: 1042 1042
Size: 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 210 244 210
Size: 34 4876
BciVI GTATCCnnnnn_n'
Cuts at: 1304 2808 3569 4910 1304
Size: 1504 761 1341 1304
Fragments arranged by size:
1504 1341 1304 761
BclI T'GATC_A
Cuts at: 2430 2430
Size: 4910
BglII A'GATC_T
Cuts at: 497 1374 2425 4462 497
Size: 877 1051 2037 945
Fragments arranged by size:
2037 1051 945 877
BlpI GC'TnA_GC
Cuts at: 756 2022 756
Size: 1266 3644
Bme1580I G_kGCm'C
Cuts at: 2535 2628 3684 2535
Size: 93 1056 3761
Fragments arranged by size:
3761 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 142 156 2122 2203 2538 4682 142
Size: 14 1966 81 335 2144 370
Fragments arranged by size:
2144 1966 370 335 81 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 771 3277 771
Size: 2506 2404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 1279 1508 403
Size: 876 229 3805
Fragments arranged by size:
3805 876 229
Bpu10I CC'TnA_GC
Cuts at: 1042 1042
Size: 4910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 834 2086 2965 3457 3755 660
Size: 174 1252 879 492 298 1815
Fragments arranged by size:
1815 1252 879 492 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 4614 4614
Size: 4910
BsaAI yAC'GTr
Cuts at: 1755 2263 1755
Size: 508 4402
BsaBI GATnn'nnATC
Cuts at: 6 2424 4461 6
Size: 2418 2037 455
Fragments arranged by size:
2418 2037 455
BsaHI Gr'CG_yC
Cuts at: 2341 2589 3291 4787 2341
Size: 248 702 1496 2464
Fragments arranged by size:
2464 1496 702 248
BsaWI w'CCGG_w
Cuts at: 849 1234 2099 2620 3298 3572 3719 4130 849
Size: 385 865 521 678 274 147 411 1629
Fragments arranged by size:
1629 865 678 521 411 385 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 641 676 1168 1534 1786 641
Size: 35 492 366 252 3765
Fragments arranged by size:
3765 492 366 252 35
BseYI C'CCAG_C
Cuts at: 688 751 2335 3093 3670 4166 688
Size: 63 1584 758 577 496 1432
Fragments arranged by size:
1584 1432 758 577 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 536 799 1023 1107 1779 536
Size: 263 224 84 672 3667
Fragments arranged by size:
3667 672 263 224 84
BsiEI CG_ry'CG
Cuts at: 104 2452 2498 3706 104
Size: 2348 46 1208 1308
Fragments arranged by size:
2348 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1924 2702 2892 3684 1924
Size: 778 190 792 3150
Fragments arranged by size:
3150 792 778 190
BsmI GAATG_Cn'
Cuts at: 682 682
Size: 4910
Bsp1286I G_dGCh'C
Cuts at: 752 1924 2535 2628 2702 2892 2954 3684 752
Size: 1172 611 93 74 190 62 730 1978
Fragments arranged by size:
1978 1172 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4130 4130
Size: 4910
BspHI T'CATG_A
Cuts at: 4086 4086
Size: 4910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1960 2174 2476 2857 1960
Size: 214 302 381 4013
Fragments arranged by size:
4013 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1476 3202 3256 4740 4905 50
Size: 1426 1726 54 1484 165 55
Fragments arranged by size:
1726 1484 1426 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2822 2822
Size: 4910
BsrFI r'CCGG_y
Cuts at: 2908 3089 3298 2908
Size: 181 209 4520
Fragments arranged by size:
4520 209 181
BsrGI T'GTAC_A
Cuts at: 1719 1719
Size: 4910
BssSI C'ACGA_G
Cuts at: 1471 3181 3539 1471
Size: 1710 358 2842
Fragments arranged by size:
2842 1710 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1010 2287 1010
Size: 1277 3633
BstBI TT'CG_AA
Cuts at: 3271 3271
Size: 4910
BstXI CCAn_nnnn'nTGG
Cuts at: 557 557
Size: 4910
BstYI r'GATC_y
Cuts at: 1 497 1374 1935 2402 2425 2760 3006 4018
Size: 496 877 561 467 23 335 246 1012
Cuts at: 4018 4104 4116 4462 1
Size: 86 12 346 449
Fragments arranged by size:
1012 877 561 496 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 976 976
Size: 4910
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2860 3041 3204 2860
Size: 181 163 4566
Fragments arranged by size:
4566 181 163
BtsI GCAGTG_nn'
Cuts at: 927 1011 1767 2153 927
Size: 84 756 386 3684
Fragments arranged by size:
3684 756 386 84
DraI TTT'AAA
Cuts at: 942 1909 942
Size: 967 3943
DrdI GACnn_nn'nnGTC
Cuts at: 2616 3474 2616
Size: 858 4052
EaeI y'GGCC_r
Cuts at: 1518 1770 2495 2669 3060 3087 4432 1518
Size: 252 725 174 391 27 1345 1996
Fragments arranged by size:
1996 1345 725 391 252 174 27
EagI C'GGCC_G
Cuts at: 2495 2495
Size: 4910
EarI CTCTTCn'nnn_
Cuts at: 146 331 1064 2933 3143 146
Size: 185 733 1869 210 1913
Fragments arranged by size:
1913 1869 733 210 185
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3118 3428 3574 4720 3118
Size: 310 146 1146 3308
Fragments arranged by size:
3308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1922 1922
Size: 4910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 110 403 478 1279 1508 2735 3167 3914 4182
Size: 293 75 801 229 1227 432 747 268
Cuts at: 4182 110
Size: 838
Fragments arranged by size:
1227 838 801 747 432 293 268 229
75
EcoNI CCTnn'n_nnAGG
Cuts at: 722 722
Size: 4910
EcoO109I rG'GnC_Cy
Cuts at: 890 2049 890
Size: 1159 3751
EcoRI G'AATT_C
Cuts at: 540 1914 540
Size: 1374 3536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1224 2929 1224
Size: 1705 3205
FspI TGC'GCA
Cuts at: 2691 2691
Size: 4910
HaeII r_GCGC'y
Cuts at: 2344 2592 3614 4593 2344
Size: 248 1022 979 2661
Fragments arranged by size:
2661 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 330 362 941 973 1189 1221 2752
Size: 32 103 32 579 32 216 32 1531
Cuts at: 2752 2782 2784 2814 195
Size: 30 2 30 2291
Fragments arranged by size:
2291 1531 579 216 103 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 484 1289 1457 1709 1949 484
Size: 805 168 252 240 3445
Fragments arranged by size:
3445 805 252 240 168
HindIII A'AGCT_T
Cuts at: 936 1104 1272 936
Size: 168 168 4574
Fragments arranged by size:
4574 168 168
KasI G'GCGC_C
Cuts at: 2340 2588 2340
Size: 248 4662
KpnI G_GTAC'C
Cuts at: 289 1930 289
Size: 1641 3269
MluI A'CGCG_T
Cuts at: 4343 4343
Size: 4910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1061 1513 3581 3765 1061
Size: 452 2068 184 2206
Fragments arranged by size:
2206 2068 452 184
MscI TGG'CCA
Cuts at: 2671 2671
Size: 4910
MslI CAynn'nnrTG
Cuts at: 1235 1403 1655 2899 3026 1235
Size: 168 252 1244 127 3119
Fragments arranged by size:
3119 1244 252 168 127
MspA1I CmG'CkG
Cuts at: 2021 2335 2695 3708 3953 2021
Size: 314 360 1013 245 2978
Fragments arranged by size:
2978 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3091 3091
Size: 4910
NarI GG'CG_CC
Cuts at: 2341 2589 2341
Size: 248 4662
NgoMIV G'CCGG_C
Cuts at: 3089 3089
Size: 4910
NspI r_CATG'y
Cuts at: 490 1963 2463 3370 490
Size: 1473 500 907 2030
Fragments arranged by size:
2030 1473 907 500
PasI CC'CwG_GG
Cuts at: 550 550
Size: 4910
PciI A'CATG_T
Cuts at: 486 3366 486
Size: 2880 2030
PflMI CCAn_nnn'nTGG
Cuts at: 172 282 644 861 172
Size: 110 362 217 4221
Fragments arranged by size:
4221 362 217 110
PmeI GTTT'AAAC
Cuts at: 1909 1909
Size: 4910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4878
PpuMI rG'GwC_Cy
Cuts at: 890 890
Size: 4910
PstI C_TGCA'G
Cuts at: 1594 1957 2642 1594
Size: 363 685 3862
Fragments arranged by size:
3862 685 363
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4910
PvuII CAG'CTG
Cuts at: 2335 2695 2335
Size: 360 4550
RsrII CG'GwC_CG
Cuts at: 3105 3105
Size: 4910
SacI G_AGCT'C
Cuts at: 1924 1924
Size: 4910
SalI G'TCGA_C
Cuts at: 1947 1947
Size: 4910
SapI GCTCTTCn'nnn_
Cuts at: 2933 3143 2933
Size: 210 4700
SbfI CC_TGCA'GG
Cuts at: 1957 1957
Size: 4910
ScaI AGT'ACT
Cuts at: 4427 4427
Size: 4910
SfcI C'TryA_G
Cuts at: 33 834 1590 1953 2638 3631 3822 33
Size: 801 756 363 685 993 191 1121
Fragments arranged by size:
1121 993 801 756 685 363 191
SfoI GGC'GCC
Cuts at: 2342 2590 2342
Size: 248 4662
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
SmaI CCC'GGG
Cuts at: 1932 1932
Size: 4910
SmlI C'TyrA_G
Cuts at: 675 813 2065 2980 3472 3734 675
Size: 138 1252 915 492 262 1851
Fragments arranged by size:
1851 1252 915 492 262 138
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4910
SphI G_CATG'C
Cuts at: 1963 2463 1963
Size: 500 4410
StyI C'CwwG_G
Cuts at: 2044 2398 2044
Size: 354 4556
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2493 2626 3085 3294 2493
Size: 133 459 209 4109
Fragments arranged by size:
4109 459 209 133
TatI w'GTAC_w
Cuts at: 489 1719 4425 489
Size: 1230 2706 974
Fragments arranged by size:
2706 1230 974
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 4878
Tth111I GACn'n_nGTC
Cuts at: 344 2707 344
Size: 2363 2547
XbaI T'CTAG_A
Cuts at: 1941 1941
Size: 4910
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2405
Size: 4910
XmaI C'CCGG_G
Cuts at: 1930 1930
Size: 4910
XmnI GAAnn'nnTTC
Cuts at: 1062 1062
Size: 4910
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmBI BssHII BstEII Bsu36I BtgI ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI