Restriction map for
pF1KB6161
(Circular) (Six-base) MAPSORT of: pF1KB6161.seq Check: 2695 from: 1 to: 4910
pF1KB6161 4910 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 171 626 1948 171
Size: 455 1322 3133
Fragments arranged by size:
3133 1322 455
Acc65I G'GTAC_C
Cuts at: 1926 1926
Size: 4910
AclI AA'CG_TT
Cuts at: 1623 4750 1623
Size: 3127 1783
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2735 3167 3914 4182 2735
Size: 432 747 268 3463
Fragments arranged by size:
3463 747 432 268
AflIII A'CryG_T
Cuts at: 2262 3366 4343 2262
Size: 1104 977 2829
Fragments arranged by size:
2829 1104 977
AgeI A'CCGG_T
Cuts at: 3298 3298
Size: 4910
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 797 797
Size: 4910
AlwNI CAG_nnn'CTG
Cuts at: 1222 2215 3782 1222
Size: 993 1567 2350
Fragments arranged by size:
2350 1567 993
ApaLI G'TGCA_C
Cuts at: 3680 3680
Size: 4910
ApoI r'AATT_y
Cuts at: 470 1183 1777 1914 4263 4519 470
Size: 713 594 137 2349 256 861
Fragments arranged by size:
2349 861 713 594 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 4910
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
AvaI C'yCGr_G
Cuts at: 1610 1930 1610
Size: 320 4590
BamHI G'GATC_C
Cuts at: 1935 1935
Size: 4910
BanI G'GyrC_C
Cuts at: 310 1926 2340 2588 2623 310
Size: 1616 414 248 35 2597
Fragments arranged by size:
2597 1616 414 248 35
BanII G_rGCy'C
Cuts at: 1924 2954 1924
Size: 1030 3880
BbeI G_GCGC'C
Cuts at: 314 2344 2592 314
Size: 2030 248 2632
Fragments arranged by size:
2632 2030 248
BbsI GAAGACnn'nnnn_
Cuts at: 1296 1296
Size: 4910
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 161 195 161
Size: 34 4876
BciVI GTATCCnnnnn_n'
Cuts at: 2808 3569 4910 2808
Size: 761 1341 2808
Fragments arranged by size:
2808 1341 761
BclI T'GATC_A
Cuts at: 1473 2430 1473
Size: 957 3953
BfrBI ATG'CAT
Cuts at: 221 1087 221
Size: 866 4044
BglI GCCn_nnn'nGGC
Cuts at: 308 308
Size: 4910
BglII A'GATC_T
Cuts at: 753 2425 4462 753
Size: 1672 2037 1201
Fragments arranged by size:
2037 1672 1201
BlpI GC'TnA_GC
Cuts at: 2022 2022
Size: 4910
Bme1580I G_kGCm'C
Cuts at: 2535 2628 3684 2535
Size: 93 1056 3761
Fragments arranged by size:
3761 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2122 2203 2538 4682 2122
Size: 81 335 2144 2350
Fragments arranged by size:
2350 2144 335 81
BmtI G_CTAG'C
Cuts at: 1195 1195
Size: 4910
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3277 3277
Size: 4910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 1226
Size: 4910
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1092 2086 2965 3457 3755 1092
Size: 994 879 492 298 2247
Fragments arranged by size:
2247 994 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4614 4614
Size: 4910
BsaAI yAC'GTr
Cuts at: 2263 2263
Size: 4910
BsaBI GATnn'nnATC
Cuts at: 6 2424 4461 6
Size: 2418 2037 455
Fragments arranged by size:
2418 2037 455
BsaHI Gr'CG_yC
Cuts at: 311 2341 2589 3291 4787 311
Size: 2030 248 702 1496 434
Fragments arranged by size:
2030 1496 702 434 248
BsaWI w'CCGG_w
Cuts at: 2099 2620 3298 3572 3719 4130 2099
Size: 521 678 274 147 411 2879
Fragments arranged by size:
2879 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 937 967 1301 1331 937
Size: 30 334 30 4516
Fragments arranged by size:
4516 334 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 779 1325 779
Size: 546 4364
BseYI C'CCAG_C
Cuts at: 1239 2335 3093 3670 4166 1239
Size: 1096 758 577 496 1983
Fragments arranged by size:
1983 1096 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1504 1504
Size: 4910
BsiEI CG_ry'CG
Cuts at: 104 2452 2498 3706 104
Size: 2348 46 1208 1308
Fragments arranged by size:
2348 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1924 2702 2892 3684 1924
Size: 778 190 792 3150
Fragments arranged by size:
3150 792 778 190
BsmI GAATG_Cn'
Cuts at: 184 1283 184
Size: 1099 3811
Bsp1286I G_dGCh'C
Cuts at: 1924 2535 2628 2702 2892 2954 3684 1924
Size: 611 93 74 190 62 730 3150
Fragments arranged by size:
3150 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4130 4130
Size: 4910
BspHI T'CATG_A
Cuts at: 4086 4086
Size: 4910
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1551 1960 2174 2476 2857 1551
Size: 409 214 302 381 3604
Fragments arranged by size:
3604 409 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3202 3256 4740 4905 50
Size: 3152 54 1484 165 55
Fragments arranged by size:
3152 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2822 2822
Size: 4910
BsrFI r'CCGG_y
Cuts at: 2908 3089 3298 2908
Size: 181 209 4520
Fragments arranged by size:
4520 209 181
BssSI C'ACGA_G
Cuts at: 3181 3539 3181
Size: 358 4552
BstAPI GCAn_nnn'nTGC
Cuts at: 2287 2287
Size: 4910
BstBI TT'CG_AA
Cuts at: 204 3271 204
Size: 3067 1843
BstYI r'GATC_y
Cuts at: 1 553 661 753 1812 1935 2402 2425 2760
Size: 552 108 92 1059 123 467 23 335
Cuts at: 2760 3006 4018 4104 4116 4462 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1059 1012 552 467 449 346 335 246
123 108 92 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 638 638
Size: 4910
BtgI C'CryG_G
Cuts at: 107 549 107
Size: 442 4468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2860 3041 3204 2860
Size: 181 163 4566
Fragments arranged by size:
4566 181 163
BtsI GCAGTG_nn'
Cuts at: 2153 2153
Size: 4910
ClaI AT'CG_AT
Cuts at: 363 363
Size: 4910
DraI TTT'AAA
Cuts at: 467 866 1101 1909 467
Size: 399 235 808 3468
Fragments arranged by size:
3468 808 399 235
DrdI GACnn_nn'nnGTC
Cuts at: 2616 3474 2616
Size: 858 4052
EaeI y'GGCC_r
Cuts at: 1679 2495 2669 3060 3087 4432 1679
Size: 816 174 391 27 1345 2157
Fragments arranged by size:
2157 1345 816 391 174 27
EagI C'GGCC_G
Cuts at: 2495 2495
Size: 4910
EarI CTCTTCn'nnn_
Cuts at: 1803 1870 2933 3143 1803
Size: 67 1063 210 3570
Fragments arranged by size:
3570 1063 210 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3118 3428 3574 4720 3118
Size: 310 146 1146 3308
Fragments arranged by size:
3308 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1922 1922
Size: 4910
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1226 2735 3167 3914 4182 1226
Size: 1509 432 747 268 1954
Fragments arranged by size:
1954 1509 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 410 1304 410
Size: 894 4016
EcoO109I rG'GnC_Cy
Cuts at: 2049 2049
Size: 4910
EcoRI G'AATT_C
Cuts at: 1914 1914
Size: 4910
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1427 1668 2929 1427
Size: 241 1261 3408
Fragments arranged by size:
3408 1261 241
FspI TGC'GCA
Cuts at: 2691 2691
Size: 4910
HaeII r_GCGC'y
Cuts at: 314 992 2344 2592 3614 4593 314
Size: 678 1352 248 1022 979 631
Fragments arranged by size:
1352 1022 979 678 631 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 937 969 1555 1587 1593 1625 2752 2782 2784
Size: 32 586 32 6 32 1127 30 2
Cuts at: 2784 2814 937
Size: 30 3033
Fragments arranged by size:
3033 1127 586 32 32 32 30 30
6 2
HincII GTy'rAC
Cuts at: 122 141 172 458 1949 122
Size: 19 31 286 1491 3083
Fragments arranged by size:
3083 1491 286 31 19
HpaI GTT'AAC
Cuts at: 122 122
Size: 4910
KasI G'GCGC_C
Cuts at: 310 2340 2588 310
Size: 2030 248 2632
Fragments arranged by size:
2632 2030 248
KpnI G_GTAC'C
Cuts at: 1930 1930
Size: 4910
MfeI C'AATT_G
Cuts at: 320 701 1196 320
Size: 381 495 4034
Fragments arranged by size:
4034 495 381
MluI A'CGCG_T
Cuts at: 4343 4343
Size: 4910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 414 980 1084 3581 3765 414
Size: 566 104 2497 184 1559
Fragments arranged by size:
2497 1559 566 184 104
MscI TGG'CCA
Cuts at: 1681 2671 1681
Size: 990 3920
MslI CAynn'nnrTG
Cuts at: 919 2899 3026 919
Size: 1980 127 2803
Fragments arranged by size:
2803 1980 127
MspA1I CmG'CkG
Cuts at: 2021 2335 2695 3708 3953 2021
Size: 314 360 1013 245 2978
Fragments arranged by size:
2978 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3091 3091
Size: 4910
NarI GG'CG_CC
Cuts at: 311 2341 2589 311
Size: 2030 248 2632
Fragments arranged by size:
2632 2030 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4910
NgoMIV G'CCGG_C
Cuts at: 3089 3089
Size: 4910
NheI G'CTAG_C
Cuts at: 1191 1191
Size: 4910
NsiI A_TGCA'T
Cuts at: 223 1089 223
Size: 866 4044
NspI r_CATG'y
Cuts at: 1963 2463 3370 1963
Size: 500 907 3503
Fragments arranged by size:
3503 907 500
PciI A'CATG_T
Cuts at: 3366 3366
Size: 4910
PflMI CCAn_nnn'nTGG
Cuts at: 499 499
Size: 4910
PfoI T'CCnGG_A
Cuts at: 1158 1158
Size: 4910
PmeI GTTT'AAAC
Cuts at: 1909 1909
Size: 4910
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4049 4081 4049
Size: 32 4878
PsiI TTA'TAA
Cuts at: 1340 1340
Size: 4910
PspXI vC'TCGA_Gb
Cuts at: 1610 1610
Size: 4910
PstI C_TGCA'G
Cuts at: 1957 2642 1957
Size: 685 4225
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4910
PvuII CAG'CTG
Cuts at: 2335 2695 2335
Size: 360 4550
RsrII CG'GwC_CG
Cuts at: 3105 3105
Size: 4910
SacI G_AGCT'C
Cuts at: 1924 1924
Size: 4910
SalI G'TCGA_C
Cuts at: 170 1947 170
Size: 1777 3133
SapI GCTCTTCn'nnn_
Cuts at: 2933 3143 2933
Size: 210 4700
SbfI CC_TGCA'GG
Cuts at: 1957 1957
Size: 4910
ScaI AGT'ACT
Cuts at: 4427 4427
Size: 4910
SfcI C'TryA_G
Cuts at: 33 197 1507 1824 1953 2638 3631 3822 33
Size: 164 1310 317 129 685 993 191 1121
Fragments arranged by size:
1310 1121 993 685 317 191 164 129
SfoI GGC'GCC
Cuts at: 312 2342 2590 312
Size: 2030 248 2632
Fragments arranged by size:
2632 2030 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4910
SmaI CCC'GGG
Cuts at: 1932 1932
Size: 4910
SmlI C'TyrA_G
Cuts at: 1071 1610 2065 2980 3472 3734 1071
Size: 539 455 915 492 262 2247
Fragments arranged by size:
2247 915 539 492 455 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4910
SphI G_CATG'C
Cuts at: 1963 2463 1963
Size: 500 4410
StyI C'CwwG_G
Cuts at: 107 498 2044 2398 107
Size: 391 1546 354 2619
Fragments arranged by size:
2619 1546 391 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 689 1436 1450 2493 2626 3085 3294 689
Size: 747 14 1043 133 459 209 2305
Fragments arranged by size:
2305 1043 747 459 209 133 14
TatI w'GTAC_w
Cuts at: 4425 4425
Size: 4910
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4427 4459 4427
Size: 32 4878
Tth111I GACn'n_nGTC
Cuts at: 2707 2707
Size: 4910
XbaI T'CTAG_A
Cuts at: 585 1941 585
Size: 1356 3554
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2405 2405
Size: 4910
XhoI C'TCGA_G
Cuts at: 1610 1610
Size: 4910
XmaI C'CCGG_G
Cuts at: 1930 1930
Size: 4910
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NsiI NspI PciI PflMI PfoI PmeI PpiI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BmgBI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII EcoRV
FseI FspAI HindIII NdeI NotI NruI PacI PasI
PmlI PpuMI PshAI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI