Restriction map for
pF1KB6163
(Circular) (Six-base) MAPSORT of: pF1KB6163.seq Check: 7890 from: 1 to: 4916
pF1KB6163 4916 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1633 1954 1633
Size: 321 4595
Acc65I G'GTAC_C
Cuts at: 1566 1932 1566
Size: 366 4550
AclI AA'CG_TT
Cuts at: 4756 4756
Size: 4916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 2741 3173 3920 4188 476
Size: 2265 432 747 268 1204
Fragments arranged by size:
2265 1204 747 432 268
AflIII A'CryG_T
Cuts at: 2268 3372 4349 2268
Size: 1104 977 2835
Fragments arranged by size:
2835 1104 977
AgeI A'CCGG_T
Cuts at: 3304 3304
Size: 4916
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1351 1361 1351
Size: 10 4906
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 970 1002 970
Size: 32 4884
AlwNI CAG_nnn'CTG
Cuts at: 385 499 1068 1794 1848 2221 3788 385
Size: 114 569 726 54 373 1567 1513
Fragments arranged by size:
1567 1513 726 569 373 114 54
ApaI G_GGCC'C
Cuts at: 536 912 536
Size: 376 4540
ApaLI G'TGCA_C
Cuts at: 1180 3686 1180
Size: 2506 2410
ApoI r'AATT_y
Cuts at: 1920 4269 4525 1920
Size: 2349 256 2311
Fragments arranged by size:
2349 2311 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4916
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
AvaI C'yCGr_G
Cuts at: 998 1135 1496 1639 1936 998
Size: 137 361 143 297 3978
Fragments arranged by size:
3978 361 297 143 137
AvrII C'CTAG_G
Cuts at: 618 618
Size: 4916
BamHI G'GATC_C
Cuts at: 556 1941 556
Size: 1385 3531
BanI G'GyrC_C
Cuts at: 1153 1566 1932 2346 2594 2629 1153
Size: 413 366 414 248 35 3440
Fragments arranged by size:
3440 414 413 366 248 35
BanII G_rGCy'C
Cuts at: 335 536 631 912 983 1091 1111 1900 1930
Size: 201 95 281 71 108 20 789 30
Cuts at: 1930 2960 335
Size: 1030 2291
Fragments arranged by size:
2291 1030 789 281 201 108 95 71
30 20
BbeI G_GCGC'C
Cuts at: 2350 2598 2350
Size: 248 4668
BbsI GAAGACnn'nnnn_
Cuts at: 1437 1437
Size: 4916
BbvCI CC'TCA_GC
Cuts at: 423 1611 423
Size: 1188 3728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1348 1382 1348
Size: 34 4882
BciVI GTATCCnnnnn_n'
Cuts at: 2814 3575 4916 2814
Size: 761 1341 2814
Fragments arranged by size:
2814 1341 761
BclI T'GATC_A
Cuts at: 2436 2436
Size: 4916
BglI GCCn_nnn'nGGC
Cuts at: 1507 1507
Size: 4916
BglII A'GATC_T
Cuts at: 1274 2431 4468 1274
Size: 1157 2037 1722
Fragments arranged by size:
2037 1722 1157
BlpI GC'TnA_GC
Cuts at: 248 2028 248
Size: 1780 3136
Bme1580I G_kGCm'C
Cuts at: 536 912 1156 1184 2541 2634 3690 536
Size: 376 244 28 1357 93 1056 1762
Fragments arranged by size:
1762 1357 1056 376 244 93 28
BmrI ACTGGGnnnn_n'
Cuts at: 557 1435 2128 2209 2544 4688 557
Size: 878 693 81 335 2144 785
Fragments arranged by size:
2144 878 785 693 335 81
BmtI G_CTAG'C
Cuts at: 1223 1223
Size: 4916
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 246 1108 1494 3283 246
Size: 862 386 1789 1879
Fragments arranged by size:
1879 1789 862 386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 956 1125 1252 1816 956
Size: 169 127 564 4056
Fragments arranged by size:
4056 564 169 127
Bpu10I CC'TnA_GC
Cuts at: 423 913 1611 423
Size: 490 698 3728
Fragments arranged by size:
3728 698 490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 356 943 2092 2971 3463 3761 274
Size: 82 587 1149 879 492 298 1429
Fragments arranged by size:
1429 1149 879 587 492 298 82
BsaI GGTCTCn'nnnn_
Cuts at: 4620 4620
Size: 4916
BsaAI yAC'GTr
Cuts at: 2269 2269
Size: 4916
BsaBI GATnn'nnATC
Cuts at: 6 2430 4467 6
Size: 2424 2037 455
Fragments arranged by size:
2424 2037 455
BsaHI Gr'CG_yC
Cuts at: 1768 2347 2595 3297 4793 1768
Size: 579 248 702 1496 1891
Fragments arranged by size:
1891 1496 702 579 248
BsaWI w'CCGG_w
Cuts at: 1141 1156 2105 2626 3304 3578 3725 4136 1141
Size: 15 949 521 678 274 147 411 1921
Fragments arranged by size:
1921 949 678 521 411 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 507 510 537 540 970 1000 1665 1695 507
Size: 3 27 3 430 30 665 30 3728
Fragments arranged by size:
3728 665 430 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 531 1255 531
Size: 724 4192
BseYI C'CCAG_C
Cuts at: 464 547 609 1061 1289 1570 1602 1703 1893
Size: 83 62 452 228 281 32 101 190
Cuts at: 1893 1901 2341 3099 3676 4172 464
Size: 8 440 758 577 496 1208
Fragments arranged by size:
1208 758 577 496 452 440 281 228
190 101 83 62 32 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1783 1783
Size: 4916
BsiEI CG_ry'CG
Cuts at: 104 198 2458 2504 3712 104
Size: 94 2260 46 1208 1308
Fragments arranged by size:
2260 1308 1208 94 46
BsiHKAI G_wGCw'C
Cuts at: 1184 1930 2708 2898 3690 1184
Size: 746 778 190 792 2410
Fragments arranged by size:
2410 792 778 746 190
BsmBI CGTCTCn'nnnn_
Cuts at: 124 124
Size: 4916
Bsp1286I G_dGCh'C
Cuts at: 335 536 631 912 983 1091 1111 1156 1184
Size: 201 95 281 71 108 20 45 28
Cuts at: 1184 1900 1930 2541 2634 2708 2898 2960 3690
Size: 716 30 611 93 74 190 62 730
Cuts at: 3690 335
Size: 1561
Fragments arranged by size:
1561 730 716 611 281 201 190 108
95 93 74 71 62 45 30 28
20
BspEI T'CCGG_A
Cuts at: 4136 4136
Size: 4916
BspHI T'CATG_A
Cuts at: 839 4092 839
Size: 3253 1663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1847 1966 2180 2482 2863 1847
Size: 119 214 302 381 3900
Fragments arranged by size:
3900 381 302 214 119
BsrBI CCG'CTC
Cuts at: 50 122 798 1392 3208 3262 4746 4911 50
Size: 72 676 594 1816 54 1484 165 55
Fragments arranged by size:
1816 1484 676 594 165 72 55 54
BsrDI GCAATG_nn'
Cuts at: 1510 2828 1510
Size: 1318 3598
BsrFI r'CCGG_y
Cuts at: 2914 3095 3304 2914
Size: 181 209 4526
Fragments arranged by size:
4526 209 181
BsrGI T'GTAC_A
Cuts at: 962 962
Size: 4916
BssSI C'ACGA_G
Cuts at: 3187 3545 3187
Size: 358 4558
BstAPI GCAn_nnn'nTGC
Cuts at: 660 1458 1512 1716 2293 660
Size: 798 54 204 577 3283
Fragments arranged by size:
3283 798 577 204 54
BstBI TT'CG_AA
Cuts at: 3277 3277
Size: 4916
BstEII G'GTnAC_C
Cuts at: 150 150
Size: 4916
BstXI CCAn_nnnn'nTGG
Cuts at: 642 642
Size: 4916
BstYI r'GATC_y
Cuts at: 1 284 375 556 1274 1520 1941 2408 2431
Size: 283 91 181 718 246 421 467 23
Cuts at: 2431 2766 3012 4024 4110 4122 4468 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 718 467 449 421 346 335 283
246 246 181 91 86 23 12
BtgI C'CryG_G
Cuts at: 107 1811 107
Size: 1704 3212
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2866 3047 3210 2866
Size: 181 163 4572
Fragments arranged by size:
4572 181 163
BtsI GCAGTG_nn'
Cuts at: 389 489 510 528 2159 389
Size: 100 21 18 1631 3146
Fragments arranged by size:
3146 1631 100 21 18
DraI TTT'AAA
Cuts at: 1915 1915
Size: 4916
DrdI GACnn_nn'nnGTC
Cuts at: 2622 3480 2622
Size: 858 4058
EaeI y'GGCC_r
Cuts at: 767 1034 2501 2675 3066 3093 4438 767
Size: 267 1467 174 391 27 1345 1245
Fragments arranged by size:
1467 1345 1245 391 267 174 27
EagI C'GGCC_G
Cuts at: 2501 2501
Size: 4916
EarI CTCTTCn'nnn_
Cuts at: 495 1032 1399 2939 3149 495
Size: 537 367 1540 210 2262
Fragments arranged by size:
2262 1540 537 367 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 943 1417 3124 3434 3580 4726 127
Size: 816 474 1707 310 146 1146 317
Fragments arranged by size:
1707 1146 816 474 317 310 146
EcoICRI GAG'CTC
Cuts at: 1928 1928
Size: 4916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 476 956 1125 1252 1816 2741 3173 3920 4188
Size: 480 169 127 564 925 432 747 268
Cuts at: 4188 476
Size: 1204
Fragments arranged by size:
1204 925 747 564 480 432 268 169
127
EcoNI CCTnn'n_nnAGG
Cuts at: 721 721
Size: 4916
EcoO109I rG'GnC_Cy
Cuts at: 137 532 533 909 1131 1554 1644 1845 2055
Size: 395 1 376 222 423 90 201 210
Cuts at: 2055 137
Size: 2998
Fragments arranged by size:
2998 423 395 376 222 210 201 90
1
EcoRI G'AATT_C
Cuts at: 1920 1920
Size: 4916
EcoRV GAT'ATC
Cuts at: 1592 1592
Size: 4916
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2935 2935
Size: 4916
FspI TGC'GCA
Cuts at: 1327 2697 1327
Size: 1370 3546
HaeII r_GCGC'y
Cuts at: 1703 2350 2598 3620 4599 1703
Size: 647 248 1022 979 2020
Fragments arranged by size:
2020 1022 979 647 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 180 187 191 212 219 223 406 438 666
Size: 7 4 21 7 4 183 32 228
Cuts at: 666 679 698 711 718 750 1039 1065 1071
Size: 13 19 13 7 32 289 26 6
Cuts at: 1071 1097 1853 1885 2758 2788 2790 2820 180
Size: 26 756 32 873 30 2 30 2276
Fragments arranged by size:
2276 873 756 289 228 183 32 32
32 30 30 26 26 21 19 13
13 7 7 7 6 4 4 2
HincII GTy'rAC
Cuts at: 1955 1955
Size: 4916
KasI G'GCGC_C
Cuts at: 2346 2594 2346
Size: 248 4668
KpnI G_GTAC'C
Cuts at: 1570 1936 1570
Size: 366 4550
MluI A'CGCG_T
Cuts at: 4349 4349
Size: 4916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1070 1708 3587 3771 1070
Size: 638 1879 184 2215
Fragments arranged by size:
2215 1879 638 184
MscI TGG'CCA
Cuts at: 769 2677 769
Size: 1908 3008
MslI CAynn'nnrTG
Cuts at: 763 2905 3032 763
Size: 2142 127 2647
Fragments arranged by size:
2647 2142 127
MspA1I CmG'CkG
Cuts at: 261 382 666 672 1293 1794 2027 2341 2701
Size: 121 284 6 621 501 233 314 360
Cuts at: 2701 3714 3959 261
Size: 1013 245 1218
Fragments arranged by size:
1218 1013 621 501 360 314 284 245
233 121 6
NaeI GCC'GGC
Cuts at: 3097 3097
Size: 4916
NarI GG'CG_CC
Cuts at: 2347 2595 2347
Size: 248 4668
NcoI C'CATG_G
Cuts at: 107 1811 107
Size: 1704 3212
NgoMIV G'CCGG_C
Cuts at: 3095 3095
Size: 4916
NheI G'CTAG_C
Cuts at: 1219 1219
Size: 4916
NspI r_CATG'y
Cuts at: 822 963 1326 1969 2469 3376 822
Size: 141 363 643 500 907 2362
Fragments arranged by size:
2362 907 643 500 363 141
PasI CC'CwG_GG
Cuts at: 1558 1558
Size: 4916
PciI A'CATG_T
Cuts at: 3372 3372
Size: 4916
PflMI CCAn_nnn'nTGG
Cuts at: 1737 1737
Size: 4916
PfoI T'CCnGG_A
Cuts at: 232 722 974 1594 232
Size: 490 252 620 3554
Fragments arranged by size:
3554 620 490 252
PmeI GTTT'AAAC
Cuts at: 1915 1915
Size: 4916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 970 1002 4055 4087 970
Size: 32 3053 32 1799
Fragments arranged by size:
3053 1799 32 32
PspOMI G'GGCC_C
Cuts at: 532 908 532
Size: 376 4540
PstI C_TGCA'G
Cuts at: 653 1418 1436 1454 1520 1844 1963 2648 653
Size: 765 18 18 66 324 119 685 2921
Fragments arranged by size:
2921 765 685 324 119 66 18 18
PvuI CG_AT'CG
Cuts at: 104 198 104
Size: 94 4822
PvuII CAG'CTG
Cuts at: 261 382 672 1293 1794 2341 2701 261
Size: 121 290 621 501 547 360 2476
Fragments arranged by size:
2476 621 547 501 360 290 121
RsrII CG'GwC_CG
Cuts at: 3111 3111
Size: 4916
SacI G_AGCT'C
Cuts at: 1930 1930
Size: 4916
SalI G'TCGA_C
Cuts at: 1953 1953
Size: 4916
SapI GCTCTTCn'nnn_
Cuts at: 1399 2939 3149 1399
Size: 1540 210 3166
Fragments arranged by size:
3166 1540 210
SbfI CC_TGCA'GG
Cuts at: 1844 1963 1844
Size: 119 4797
ScaI AGT'ACT
Cuts at: 4433 4433
Size: 4916
SfcI C'TryA_G
Cuts at: 33 649 1414 1432 1450 1516 1534 1840 1959
Size: 616 765 18 18 66 18 306 119
Cuts at: 1959 2644 3637 3828 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 765 685 616 306 191 119
66 18 18 18
SfoI GGC'GCC
Cuts at: 2348 2596 2348
Size: 248 4668
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4916
SmaI CCC'GGG
Cuts at: 1000 1137 1938 1000
Size: 137 801 3978
Fragments arranged by size:
3978 801 137
SmlI C'TyrA_G
Cuts at: 289 371 922 2071 2986 3478 3740 289
Size: 82 551 1149 915 492 262 1465
Fragments arranged by size:
1465 1149 915 551 492 262 82
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4916
SphI G_CATG'C
Cuts at: 1969 2469 1969
Size: 500 4416
StuI AGG'CCT
Cuts at: 623 623
Size: 4916
StyI C'CwwG_G
Cuts at: 107 167 618 1575 1811 2050 2404 107
Size: 60 451 957 236 239 354 2619
Fragments arranged by size:
2619 957 451 354 239 236 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 195 1564 2499 2632 3091 3300 195
Size: 1369 935 133 459 209 1811
Fragments arranged by size:
1811 1369 935 459 209 133
TatI w'GTAC_w
Cuts at: 962 4431 962
Size: 3469 1447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 507 510 539 542 1466 1498 1665 1697 4433
Size: 3 29 3 924 32 167 32 2736
Cuts at: 4433 4465 507
Size: 32 958
Fragments arranged by size:
2736 958 924 167 32 32 32 29
3 3
Tth111I GACn'n_nGTC
Cuts at: 2713 2713
Size: 4916
XbaI T'CTAG_A
Cuts at: 1947 1947
Size: 4916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1738 2411 1738
Size: 673 4243
XmaI C'CCGG_G
Cuts at: 998 1135 1936 998
Size: 137 801 3978
Fragments arranged by size:
3978 801 137
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AscI BaeI
BfrBI BmgBI BsiWI BsmI BssHII BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NdeI
NotI NruI NsiI PacI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI