Restriction map for
pF1KB6164
(Circular) (Six-base) MAPSORT of: pF1KB6164.seq Check: 9892 from: 1 to: 4919
pF1KB6164 4919 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 1277 1277
Size: 4919
AccI GT'mk_AC
Cuts at: 1121 1957 1121
Size: 836 4083
Acc65I G'GTAC_C
Cuts at: 1935 1935
Size: 4919
AclI AA'CG_TT
Cuts at: 4759 4759
Size: 4919
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1537 1768 2744 3176 3923 4191 1537
Size: 231 976 432 747 268 2265
Fragments arranged by size:
2265 976 747 432 268 231
AflIII A'CryG_T
Cuts at: 1841 2271 3375 4352 1841
Size: 430 1104 977 2408
Fragments arranged by size:
2408 1104 977 430
AgeI A'CCGG_T
Cuts at: 3307 3307
Size: 4919
AhdI GACnn_n'nnGTC
Cuts at: 501 501
Size: 4919
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1228 1260 1301 1333 1228
Size: 32 41 32 4814
Fragments arranged by size:
4814 41 32 32
AlwNI CAG_nnn'CTG
Cuts at: 682 1545 1746 2224 3791 682
Size: 863 201 478 1567 1810
Fragments arranged by size:
1810 1567 863 478 201
ApaI G_GGCC'C
Cuts at: 341 341
Size: 4919
ApaLI G'TGCA_C
Cuts at: 3689 3689
Size: 4919
ApoI r'AATT_y
Cuts at: 526 1923 4272 4528 526
Size: 1397 2349 256 917
Fragments arranged by size:
2349 1397 917 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4919
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4919
AvaI C'yCGr_G
Cuts at: 177 863 1427 1939 177
Size: 686 564 512 3157
Fragments arranged by size:
3157 686 564 512
BamHI G'GATC_C
Cuts at: 858 1944 858
Size: 1086 3833
BanI G'GyrC_C
Cuts at: 1935 2349 2597 2632 1935
Size: 414 248 35 4222
Fragments arranged by size:
4222 414 248 35
BanII G_rGCy'C
Cuts at: 140 341 710 923 1007 1680 1933 2963 140
Size: 201 369 213 84 673 253 1030 2096
Fragments arranged by size:
2096 1030 673 369 253 213 201 84
BbeI G_GCGC'C
Cuts at: 2353 2601 2353
Size: 248 4671
BbvCI CC'TCA_GC
Cuts at: 388 1130 1730 388
Size: 742 600 3577
Fragments arranged by size:
3577 742 600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4885
BciVI GTATCCnnnnn_n'
Cuts at: 1673 2817 3578 4919 1673
Size: 1144 761 1341 1673
Fragments arranged by size:
1673 1341 1144 761
BclI T'GATC_A
Cuts at: 2439 2439
Size: 4919
BglII A'GATC_T
Cuts at: 2434 4471 2434
Size: 2037 2882
BlpI GC'TnA_GC
Cuts at: 313 1001 2031 313
Size: 688 1030 3201
Fragments arranged by size:
3201 1030 688
Bme1580I G_kGCm'C
Cuts at: 341 2544 2637 3693 341
Size: 2203 93 1056 1567
Fragments arranged by size:
2203 1567 1056 93
BmgBI CAC'GTC
Cuts at: 1842 1842
Size: 4919
BmrI ACTGGGnnnn_n'
Cuts at: 246 1653 2131 2212 2547 4691 246
Size: 1407 478 81 335 2144 474
Fragments arranged by size:
2144 1407 478 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 4919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1030 1180 1279 1882 721
Size: 309 150 99 603 3758
Fragments arranged by size:
3758 603 309 150 99
Bpu10I CC'TnA_GC
Cuts at: 388 1130 1730 388
Size: 742 600 3577
Fragments arranged by size:
3577 742 600
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 790 865 1189 2095 2974 3466 3764 790
Size: 75 324 906 879 492 298 1945
Fragments arranged by size:
1945 906 879 492 324 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 1711 4623 1711
Size: 2912 2007
BsaAI yAC'GTr
Cuts at: 2272 2272
Size: 4919
BsaBI GATnn'nnATC
Cuts at: 6 2433 4470 6
Size: 2427 2037 455
Fragments arranged by size:
2427 2037 455
BsaHI Gr'CG_yC
Cuts at: 1274 2350 2598 3300 4796 1274
Size: 1076 248 702 1496 1397
Fragments arranged by size:
1496 1397 1076 702 248
BsaWI w'CCGG_w
Cuts at: 647 2108 2629 3307 3581 3728 4139 647
Size: 1461 521 678 274 147 411 1427
Fragments arranged by size:
1461 1427 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 818 848 1228 1258 1450 1480 442
Size: 30 346 30 380 30 192 30 3881
Fragments arranged by size:
3881 380 346 192 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 223 363 466 1276 1651 223
Size: 140 103 810 375 3491
Fragments arranged by size:
3491 810 375 140 103
BseYI C'CCAG_C
Cuts at: 215 227 233 239 283 2344 3102 3679 4175
Size: 12 6 6 44 2061 758 577 496
Cuts at: 4175 215
Size: 959
Fragments arranged by size:
2061 959 758 577 496 44 12 6
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1436 1542 1436
Size: 106 4813
BsiEI CG_ry'CG
Cuts at: 104 2461 2507 3715 104
Size: 2357 46 1208 1308
Fragments arranged by size:
2357 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1007 1064 1374 1933 2711 2901 3693 1007
Size: 57 310 559 778 190 792 2233
Fragments arranged by size:
2233 792 778 559 310 190 57
BsmBI CGTCTCn'nnnn_
Cuts at: 970 970
Size: 4919
Bsp1286I G_dGCh'C
Cuts at: 140 341 710 923 1007 1064 1374 1680 1933
Size: 201 369 213 84 57 310 306 253
Cuts at: 1933 2544 2637 2711 2901 2963 3693 140
Size: 611 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 611 369 310 306 253 213
201 190 93 84 74 62 57
BspEI T'CCGG_A
Cuts at: 647 4139 647
Size: 3492 1427
BspHI T'CATG_A
Cuts at: 1400 4095 1400
Size: 2695 2224
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1969 2183 2485 2866 1969
Size: 214 302 381 4022
Fragments arranged by size:
4022 381 302 214
BsrBI CCG'CTC
Cuts at: 50 447 870 3211 3265 4749 4914 50
Size: 397 423 2341 54 1484 165 55
Fragments arranged by size:
2341 1484 423 397 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2831 2831
Size: 4919
BsrFI r'CCGG_y
Cuts at: 1185 1592 2917 3098 3307 1185
Size: 407 1325 181 209 2797
Fragments arranged by size:
2797 1325 407 209 181
BsrGI T'GTAC_A
Cuts at: 1844 1844
Size: 4919
BssSI C'ACGA_G
Cuts at: 742 3190 3548 742
Size: 2448 358 2113
Fragments arranged by size:
2448 2113 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1369 2296 1369
Size: 927 3992
BstBI TT'CG_AA
Cuts at: 3280 3280
Size: 4919
BstYI r'GATC_y
Cuts at: 1 858 1013 1944 2411 2434 2769 3015 4027
Size: 857 155 931 467 23 335 246 1012
Cuts at: 4027 4113 4125 4471 1
Size: 86 12 346 449
Fragments arranged by size:
1012 931 857 467 449 346 335 246
155 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 614 614
Size: 4919
BtgI C'CryG_G
Cuts at: 1494 1494
Size: 4919
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 880 2869 3050 3213 880
Size: 1989 181 163 2586
Fragments arranged by size:
2586 1989 181 163
BtsI GCAGTG_nn'
Cuts at: 1687 2162 1687
Size: 475 4444
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 171 206 171
Size: 35 4884
DraI TTT'AAA
Cuts at: 1918 1918
Size: 4919
DraIII CAC_nnn'GTG
Cuts at: 517 517
Size: 4919
DrdI GACnn_nn'nnGTC
Cuts at: 2625 3483 2625
Size: 858 4061
EaeI y'GGCC_r
Cuts at: 727 1328 2504 2678 3069 3096 4441 727
Size: 601 1176 174 391 27 1345 1205
Fragments arranged by size:
1345 1205 1176 601 391 174 27
EagI C'GGCC_G
Cuts at: 2504 2504
Size: 4919
EarI CTCTTCn'nnn_
Cuts at: 649 1052 1321 1610 1744 1877 2942 3152 649
Size: 403 269 289 134 133 1065 210 2416
Fragments arranged by size:
2416 1065 403 289 269 210 134 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1309 1894 3127 3437 3583 4729 1309
Size: 585 1233 310 146 1146 1499
Fragments arranged by size:
1499 1233 1146 585 310 146
EcoICRI GAG'CTC
Cuts at: 1005 1931 1005
Size: 926 3993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1030 1180 1279 1537 1768 1882 2744 3176
Size: 309 150 99 258 231 114 862 432
Cuts at: 3176 3923 4191 721
Size: 747 268 1449
Fragments arranged by size:
1449 862 747 432 309 268 258 231
150 114 99
EcoNI CCTnn'n_nnAGG
Cuts at: 1099 1162 1099
Size: 63 4856
EcoO109I rG'GnC_Cy
Cuts at: 337 338 2058 337
Size: 1 1720 3198
Fragments arranged by size:
3198 1720 1
EcoRI G'AATT_C
Cuts at: 1923 1923
Size: 4919
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4919
FspI TGC'GCA
Cuts at: 2700 2700
Size: 4919
HaeII r_GCGC'y
Cuts at: 2353 2601 3623 4602 2353
Size: 248 1022 979 2670
Fragments arranged by size:
2670 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 487 519 969 1001 2761 2791 2793 2823 487
Size: 32 450 32 1760 30 2 30 2583
Fragments arranged by size:
2583 1760 450 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1958 1958
Size: 4919
HindIII A'AGCT_T
Cuts at: 412 412
Size: 4919
KasI G'GCGC_C
Cuts at: 2349 2597 2349
Size: 248 4671
KpnI G_GTAC'C
Cuts at: 1939 1939
Size: 4919
MluI A'CGCG_T
Cuts at: 4352 4352
Size: 4919
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 296 1805 3590 3774 296
Size: 1509 1785 184 1441
Fragments arranged by size:
1785 1509 1441 184
MscI TGG'CCA
Cuts at: 729 1330 2680 729
Size: 601 1350 2968
Fragments arranged by size:
2968 1350 601
MslI CAynn'nnrTG
Cuts at: 584 2908 3035 584
Size: 2324 127 2468
Fragments arranged by size:
2468 2324 127
MspA1I CmG'CkG
Cuts at: 1134 1326 1729 2030 2344 2704 3717 3962 1134
Size: 192 403 301 314 360 1013 245 2091
Fragments arranged by size:
2091 1013 403 360 314 301 245 192
NaeI GCC'GGC
Cuts at: 3100 3100
Size: 4919
NarI GG'CG_CC
Cuts at: 2350 2598 2350
Size: 248 4671
NcoI C'CATG_G
Cuts at: 1494 1494
Size: 4919
NgoMIV G'CCGG_C
Cuts at: 3098 3098
Size: 4919
NspI r_CATG'y
Cuts at: 1972 2472 3379 1972
Size: 500 907 3512
Fragments arranged by size:
3512 907 500
PciI A'CATG_T
Cuts at: 3375 3375
Size: 4919
PfoI T'CCnGG_A
Cuts at: 1007 1157 1007
Size: 150 4769
PmeI GTTT'AAAC
Cuts at: 1918 1918
Size: 4919
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1228 1260 4058 4090 1228
Size: 32 2798 32 2057
Fragments arranged by size:
2798 2057 32 32
PspOMI G'GGCC_C
Cuts at: 337 337
Size: 4919
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1378 1410 1654 1686 1378
Size: 32 244 32 4611
Fragments arranged by size:
4611 244 32 32
PstI C_TGCA'G
Cuts at: 410 1966 2651 410
Size: 1556 685 2678
Fragments arranged by size:
2678 1556 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4919
PvuII CAG'CTG
Cuts at: 1134 1326 1729 2344 2704 1134
Size: 192 403 615 360 3349
Fragments arranged by size:
3349 615 403 360 192
RsrII CG'GwC_CG
Cuts at: 3114 3114
Size: 4919
SacI G_AGCT'C
Cuts at: 1007 1933 1007
Size: 926 3993
SalI G'TCGA_C
Cuts at: 1956 1956
Size: 4919
SapI GCTCTTCn'nnn_
Cuts at: 1052 2942 3152 1052
Size: 1890 210 2819
Fragments arranged by size:
2819 1890 210
SbfI CC_TGCA'GG
Cuts at: 1966 1966
Size: 4919
ScaI AGT'ACT
Cuts at: 328 4436 328
Size: 4108 811
SfcI C'TryA_G
Cuts at: 33 406 960 1458 1962 2647 3640 3831 33
Size: 373 554 498 504 685 993 191 1121
Fragments arranged by size:
1121 993 685 554 504 498 373 191
SfoI GGC'GCC
Cuts at: 2351 2599 2351
Size: 248 4671
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4919
SmaI CCC'GGG
Cuts at: 1941 1941
Size: 4919
SmlI C'TyrA_G
Cuts at: 769 863 880 1204 2074 2989 3481 3743 769
Size: 94 17 324 870 915 492 262 1945
Fragments arranged by size:
1945 915 870 492 324 262 94 17
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4919
SphI G_CATG'C
Cuts at: 1972 2472 1972
Size: 500 4419
StyI C'CwwG_G
Cuts at: 143 1232 1494 2053 2407 143
Size: 1089 262 559 354 2655
Fragments arranged by size:
2655 1089 559 354 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 592 2502 2635 3094 3303 592
Size: 1910 133 459 209 2208
Fragments arranged by size:
2208 1910 459 209 133
TatI w'GTAC_w
Cuts at: 326 569 806 825 1394 1700 1844 4434 326
Size: 243 237 19 569 306 144 2590 811
Fragments arranged by size:
2590 811 569 306 243 237 144 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1606 1638 4436 4468 1606
Size: 32 2798 32 2057
Fragments arranged by size:
2798 2057 32 32
Tth111I GACn'n_nGTC
Cuts at: 2716 2716
Size: 4919
XbaI T'CTAG_A
Cuts at: 1950 1950
Size: 4919
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1857 2414 1857
Size: 557 4362
XhoI C'TCGA_G
Cuts at: 863 863
Size: 4919
XmaI C'CCGG_G
Cuts at: 1939 1939
Size: 4919
XmnI GAAnn'nnTTC
Cuts at: 643 643
Size: 4919
ZraI GAC'GTC
Cuts at: 1275 1275
Size: 4919
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AscI AvrII BaeI
BbsI BfrBI BglI BmtI BsiWI BsmI BssHII BstEII
BstXI BstZ17I ClaI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI