Restriction map for
pF1KB6168
(Circular) (Six-base) MAPSORT of: pF1KB6168.seq Check: 5681 from: 1 to: 4922
pF1KB6168 4922 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1066 1576 1066
Size: 510 4412
AccI GT'mk_AC
Cuts at: 832 1217 1960 832
Size: 385 743 3794
Fragments arranged by size:
3794 743 385
Acc65I G'GTAC_C
Cuts at: 1938 1938
Size: 4922
AclI AA'CG_TT
Cuts at: 4762 4762
Size: 4922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1600 2747 3179 3926 4194 949
Size: 651 1147 432 747 268 1677
Fragments arranged by size:
1677 1147 747 651 432 268
AflIII A'CryG_T
Cuts at: 2274 3378 4355 2274
Size: 1104 977 2841
Fragments arranged by size:
2841 1104 977
AgeI A'CCGG_T
Cuts at: 3310 3310
Size: 4922
AleI CACnn'nnGTG
Cuts at: 1229 1864 1229
Size: 635 4287
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4922
AlwNI CAG_nnn'CTG
Cuts at: 124 606 1248 2227 3794 124
Size: 482 642 979 1567 1252
Fragments arranged by size:
1567 1252 979 642 482
ApaLI G'TGCA_C
Cuts at: 3692 3692
Size: 4922
ApoI r'AATT_y
Cuts at: 1926 4275 4531 1926
Size: 2349 256 2317
Fragments arranged by size:
2349 2317 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4922
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
AvaI C'yCGr_G
Cuts at: 766 1172 1942 766
Size: 406 770 3746
Fragments arranged by size:
3746 770 406
BamHI G'GATC_C
Cuts at: 1311 1947 1311
Size: 636 4286
BanI G'GyrC_C
Cuts at: 144 1588 1938 2352 2600 2635 144
Size: 1444 350 414 248 35 2431
Fragments arranged by size:
2431 1444 414 350 248 35
BanII G_rGCy'C
Cuts at: 198 503 1622 1885 1936 2966 198
Size: 305 1119 263 51 1030 2154
Fragments arranged by size:
2154 1119 1030 305 263 51
BbeI G_GCGC'C
Cuts at: 2356 2604 2356
Size: 248 4674
BbsI GAAGACnn'nnnn_
Cuts at: 739 739
Size: 4922
BbvCI CC'TCA_GC
Cuts at: 1307 1681 1307
Size: 374 4548
BciVI GTATCCnnnnn_n'
Cuts at: 2820 3581 4922 2820
Size: 761 1341 2820
Fragments arranged by size:
2820 1341 761
BclI T'GATC_A
Cuts at: 2442 2442
Size: 4922
BglII A'GATC_T
Cuts at: 314 666 2437 4474 314
Size: 352 1771 2037 762
Fragments arranged by size:
2037 1771 762 352
BlpI GC'TnA_GC
Cuts at: 775 1879 2034 775
Size: 1104 155 3663
Fragments arranged by size:
3663 1104 155
Bme1580I G_kGCm'C
Cuts at: 147 791 1741 2547 2640 3696 147
Size: 644 950 806 93 1056 1373
Fragments arranged by size:
1373 1056 950 806 644 93
BmgBI CAC'GTC
Cuts at: 444 444
Size: 4922
BmrI ACTGGGnnnn_n'
Cuts at: 2134 2215 2550 4694 2134
Size: 81 335 2144 2362
Fragments arranged by size:
2362 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3289 3289
Size: 4922
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 691 1828 544
Size: 147 1137 3638
Fragments arranged by size:
3638 1137 147
Bpu10I CC'TnA_GC
Cuts at: 1163 1307 1681 1163
Size: 144 374 4404
Fragments arranged by size:
4404 374 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 622 1891 2098 2977 3469 3767 361
Size: 261 1269 207 879 492 298 1516
Fragments arranged by size:
1516 1269 879 492 298 261 207
BsaI GGTCTCn'nnnn_
Cuts at: 521 626 874 4626 521
Size: 105 248 3752 817
Fragments arranged by size:
3752 817 248 105
BsaAI yAC'GTr
Cuts at: 2275 2275
Size: 4922
BsaBI GATnn'nnATC
Cuts at: 6 2436 4473 6
Size: 2430 2037 455
Fragments arranged by size:
2430 2037 455
BsaHI Gr'CG_yC
Cuts at: 2353 2601 3303 4799 2353
Size: 248 702 1496 2476
Fragments arranged by size:
2476 1496 702 248
BsaWI w'CCGG_w
Cuts at: 890 1115 2111 2632 3310 3584 3731 4142 890
Size: 225 996 521 678 274 147 411 1670
Fragments arranged by size:
1670 996 678 521 411 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 385 415 385
Size: 30 4892
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 552 1315 1825 552
Size: 763 510 3649
Fragments arranged by size:
3649 763 510
BseYI C'CCAG_C
Cuts at: 642 1093 1108 2347 3105 3682 4178 642
Size: 451 15 1239 758 577 496 1386
Fragments arranged by size:
1386 1239 758 577 496 451 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 592 1234 1660 1717 514
Size: 78 642 426 57 3719
Fragments arranged by size:
3719 642 426 78 57
BsiEI CG_ry'CG
Cuts at: 104 152 2464 2510 3718 104
Size: 48 2312 46 1208 1308
Fragments arranged by size:
2312 1308 1208 48 46
BsiHKAI G_wGCw'C
Cuts at: 198 503 1412 1574 1622 1885 1936 2714 2904
Size: 305 909 162 48 263 51 778 190
Cuts at: 2904 3696 198
Size: 792 1424
Fragments arranged by size:
1424 909 792 778 305 263 190 162
51 48
BsmBI CGTCTCn'nnnn_
Cuts at: 1051 1909 1051
Size: 858 4064
Bsp1286I G_dGCh'C
Cuts at: 147 198 503 791 1412 1574 1622 1741 1885
Size: 51 305 288 621 162 48 119 144
Cuts at: 1885 1936 2547 2640 2714 2904 2966 3696 147
Size: 51 611 93 74 190 62 730 1373
Fragments arranged by size:
1373 730 621 611 305 288 190 162
144 119 93 74 62 51 51 48
BspEI T'CCGG_A
Cuts at: 890 4142 890
Size: 3252 1670
BspHI T'CATG_A
Cuts at: 4098 4098
Size: 4922
BspMI ACCTGCnnnn'nnnn_
Cuts at: 614 1066 1576 1972 2186 2488 2869 614
Size: 452 510 396 214 302 381 2667
Fragments arranged by size:
2667 510 452 396 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3214 3268 4752 4917 50
Size: 3164 54 1484 165 55
Fragments arranged by size:
3164 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2834 2834
Size: 4922
BsrFI r'CCGG_y
Cuts at: 147 213 254 1841 2920 3101 3310 147
Size: 66 41 1587 1079 181 209 1759
Fragments arranged by size:
1759 1587 1079 209 181 66 41
BssSI C'ACGA_G
Cuts at: 3193 3551 3193
Size: 358 4564
BstAPI GCAn_nnn'nTGC
Cuts at: 124 588 1248 2299 124
Size: 464 660 1051 2747
Fragments arranged by size:
2747 1051 660 464
BstBI TT'CG_AA
Cuts at: 3283 3283
Size: 4922
BstXI CCAn_nnnn'nTGG
Cuts at: 1101 1101
Size: 4922
BstYI r'GATC_y
Cuts at: 1 224 314 666 972 1311 1947 2414 2437
Size: 223 90 352 306 339 636 467 23
Cuts at: 2437 2772 3018 4030 4116 4128 4474 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 636 467 449 352 346 339 335
306 246 223 90 86 23 12
BstZ17I GTA'TAC
Cuts at: 833 833
Size: 4922
Bsu36I CC'TnA_GG
Cuts at: 560 560
Size: 4922
BtgI C'CryG_G
Cuts at: 404 456 935 404
Size: 52 479 4391
Fragments arranged by size:
4391 479 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2872 3053 3216 2872
Size: 181 163 4578
Fragments arranged by size:
4578 181 163
BtsI GCAGTG_nn'
Cuts at: 125 977 2165 125
Size: 852 1188 2882
Fragments arranged by size:
2882 1188 852
DraI TTT'AAA
Cuts at: 1921 1921
Size: 4922
DrdI GACnn_nn'nnGTC
Cuts at: 2628 3486 2628
Size: 858 4064
EaeI y'GGCC_r
Cuts at: 149 938 1835 1892 2507 2681 3072 3099 4444
Size: 789 897 57 615 174 391 27 1345
Cuts at: 4444 149
Size: 627
Fragments arranged by size:
1345 897 789 627 615 391 174 57
27
EagI C'GGCC_G
Cuts at: 149 2507 149
Size: 2358 2564
EarI CTCTTCn'nnn_
Cuts at: 1417 2945 3155 1417
Size: 1528 210 3184
Fragments arranged by size:
3184 1528 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3130 3440 3586 4732 3130
Size: 310 146 1146 3320
Fragments arranged by size:
3320 1146 310 146
EcoICRI GAG'CTC
Cuts at: 196 501 1620 1883 1934 196
Size: 305 1119 263 51 3184
Fragments arranged by size:
3184 1119 305 263 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 544 691 949 1600 1828 2747 3179 3926 4194
Size: 147 258 651 228 919 432 747 268
Cuts at: 4194 544
Size: 1272
Fragments arranged by size:
1272 919 747 651 432 268 258 228
147
EcoO109I rG'GnC_Cy
Cuts at: 171 234 2061 171
Size: 63 1827 3032
Fragments arranged by size:
3032 1827 63
EcoRI G'AATT_C
Cuts at: 1926 1926
Size: 4922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 312 1749 1797 2941 312
Size: 1437 48 1144 2293
Fragments arranged by size:
2293 1437 1144 48
FspI TGC'GCA
Cuts at: 2703 2703
Size: 4922
HaeII r_GCGC'y
Cuts at: 264 1667 2356 2604 3626 4605 264
Size: 1403 689 248 1022 979 581
Fragments arranged by size:
1403 1022 979 689 581 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 796 828 1494 1526 2764 2794 2796
Size: 32 379 32 666 32 1238 30 2
Cuts at: 2796 2826 385
Size: 30 2481
Fragments arranged by size:
2481 1238 666 379 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1218 1961 1218
Size: 743 4179
KasI G'GCGC_C
Cuts at: 2352 2600 2352
Size: 248 4674
KpnI G_GTAC'C
Cuts at: 1942 1942
Size: 4922
MluI A'CGCG_T
Cuts at: 4355 4355
Size: 4922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 476 3593 3777 476
Size: 3117 184 1621
Fragments arranged by size:
3117 1621 184
MscI TGG'CCA
Cuts at: 1837 2683 1837
Size: 846 4076
MslI CAynn'nnrTG
Cuts at: 1229 1864 2911 3038 1229
Size: 635 1047 127 3113
Fragments arranged by size:
3113 1047 635 127
MspA1I CmG'CkG
Cuts at: 1248 2033 2347 2707 3720 3965 1248
Size: 785 314 360 1013 245 2205
Fragments arranged by size:
2205 1013 785 360 314 245
NaeI GCC'GGC
Cuts at: 256 1843 3103 256
Size: 1587 1260 2075
Fragments arranged by size:
2075 1587 1260
NarI GG'CG_CC
Cuts at: 2353 2601 2353
Size: 248 4674
NcoI C'CATG_G
Cuts at: 404 404
Size: 4922
NgoMIV G'CCGG_C
Cuts at: 254 1841 3101 254
Size: 1587 1260 2075
Fragments arranged by size:
2075 1587 1260
NspI r_CATG'y
Cuts at: 1239 1975 2475 3382 1239
Size: 736 500 907 2779
Fragments arranged by size:
2779 907 736 500
PasI CC'CwG_GG
Cuts at: 157 157
Size: 4922
PciI A'CATG_T
Cuts at: 3378 3378
Size: 4922
PflMI CCAn_nnn'nTGG
Cuts at: 1334 1334
Size: 4922
PfoI T'CCnGG_A
Cuts at: 189 227 521 1712 1805 189
Size: 38 294 1191 93 3306
Fragments arranged by size:
3306 1191 294 93 38
PmeI GTTT'AAAC
Cuts at: 1921 1921
Size: 4922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 4061
Size: 32 4890
PpuMI rG'GwC_Cy
Cuts at: 171 234 171
Size: 63 4859
PstI C_TGCA'G
Cuts at: 120 129 1244 1463 1969 2654 120
Size: 9 1115 219 506 685 2388
Fragments arranged by size:
2388 1115 685 506 219 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4922
PvuII CAG'CTG
Cuts at: 1248 2347 2707 1248
Size: 1099 360 3463
Fragments arranged by size:
3463 1099 360
RsrII CG'GwC_CG
Cuts at: 3117 3117
Size: 4922
SacI G_AGCT'C
Cuts at: 198 503 1622 1885 1936 198
Size: 305 1119 263 51 3184
Fragments arranged by size:
3184 1119 305 263 51
SalI G'TCGA_C
Cuts at: 1216 1959 1216
Size: 743 4179
SanDI GG'GwC_CC
Cuts at: 171 171
Size: 4922
SapI GCTCTTCn'nnn_
Cuts at: 1417 2945 3155 1417
Size: 1528 210 3184
Fragments arranged by size:
3184 1528 210
SbfI CC_TGCA'GG
Cuts at: 1969 1969
Size: 4922
ScaI AGT'ACT
Cuts at: 4439 4439
Size: 4922
SexAI A'CCwGG_T
Cuts at: 1645 1645
Size: 4922
SfcI C'TryA_G
Cuts at: 33 116 125 1240 1459 1965 2650 3643 3834
Size: 83 9 1115 219 506 685 993 191
Cuts at: 3834 33
Size: 1121
Fragments arranged by size:
1121 1115 993 685 506 219 191 83
9
SfoI GGC'GCC
Cuts at: 2354 2602 2354
Size: 248 4674
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4922
SgrAI Cr'CCGG_yG
Cuts at: 213 213
Size: 4922
SmaI CCC'GGG
Cuts at: 1174 1944 1174
Size: 770 4152
SmlI C'TyrA_G
Cuts at: 376 637 1906 2077 2992 3484 3746 376
Size: 261 1269 171 915 492 262 1552
Fragments arranged by size:
1552 1269 915 492 262 261 171
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4922
SphI G_CATG'C
Cuts at: 1975 2475 1975
Size: 500 4422
SspI AAT'ATT
Cuts at: 1549 1549
Size: 4922
StuI AGG'CCT
Cuts at: 1912 1912
Size: 4922
StyI C'CwwG_G
Cuts at: 332 404 1075 1374 2056 2410 332
Size: 72 671 299 682 354 2844
Fragments arranged by size:
2844 682 671 354 299 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 809 1338 2505 2638 3097 3306 809
Size: 529 1167 133 459 209 2425
Fragments arranged by size:
2425 1167 529 459 209 133
TatI w'GTAC_w
Cuts at: 1463 4437 1463
Size: 2974 1948
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1418 1450 4439 4471 1418
Size: 32 2989 32 1869
Fragments arranged by size:
2989 1869 32 32
Tth111I GACn'n_nGTC
Cuts at: 2719 2719
Size: 4922
XbaI T'CTAG_A
Cuts at: 1953 1953
Size: 4922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2417 2417
Size: 4922
XmaI C'CCGG_G
Cuts at: 1172 1942 1172
Size: 770 4152
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsiWI BsmI BsrGI
BssHII BstEII ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SfiI SnaBI SrfI SwaI XhoI XmnI ZraI