Restriction map for
pF1KB6169
(Circular) (Six-base) MAPSORT of: pF1KB6169.seq Check: 6984 from: 1 to: 5063
pF1KB6169 5063 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 542 2101 542
Size: 1559 3504
Acc65I G'GTAC_C
Cuts at: 1160 2079 1160
Size: 919 4144
AclI AA'CG_TT
Cuts at: 4903 4903
Size: 5063
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1755 2888 3320 4067 4335 1283
Size: 472 1133 432 747 268 2011
Fragments arranged by size:
2011 1133 747 472 432 268
AflIII A'CryG_T
Cuts at: 2415 3519 4496 2415
Size: 1104 977 2982
Fragments arranged by size:
2982 1104 977
AgeI A'CCGG_T
Cuts at: 3451 3451
Size: 5063
AhdI GACnn_n'nnGTC
Cuts at: 1773 1803 1773
Size: 30 5033
AlwNI CAG_nnn'CTG
Cuts at: 364 2368 3935 364
Size: 2004 1567 1492
Fragments arranged by size:
2004 1567 1492
ApaI G_GGCC'C
Cuts at: 875 1147 1456 1581 875
Size: 272 309 125 4357
Fragments arranged by size:
4357 309 272 125
ApaLI G'TGCA_C
Cuts at: 166 354 3833 166
Size: 188 3479 1396
Fragments arranged by size:
3479 1396 188
ApoI r'AATT_y
Cuts at: 2067 4416 4672 2067
Size: 2349 256 2458
Fragments arranged by size:
2458 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5063
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
AvaI C'yCGr_G
Cuts at: 1021 1448 1574 1728 1748 1778 1823 1832 2003
Size: 427 126 154 20 30 45 9 171
Cuts at: 2003 2083 1021
Size: 80 4001
Fragments arranged by size:
4001 427 171 154 126 80 45 30
20 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1803 1836 1803
Size: 33 5030
BamHI G'GATC_C
Cuts at: 2088 2088
Size: 5063
BanI G'GyrC_C
Cuts at: 950 989 1160 2079 2493 2741 2776 950
Size: 39 171 919 414 248 35 3237
Fragments arranged by size:
3237 919 414 248 171 39 35
BanII G_rGCy'C
Cuts at: 275 292 815 851 875 1147 1456 1532 1581
Size: 17 523 36 24 272 309 76 49
Cuts at: 1581 2077 3107 275
Size: 496 1030 2231
Fragments arranged by size:
2231 1030 523 496 309 272 76 49
36 24 17
BbeI G_GCGC'C
Cuts at: 2497 2745 2497
Size: 248 4815
BbsI GAAGACnn'nnnn_
Cuts at: 318 887 318
Size: 569 4494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5029
BciVI GTATCCnnnnn_n'
Cuts at: 1165 2961 3722 5063 1165
Size: 1796 761 1341 1165
Fragments arranged by size:
1796 1341 1165 761
BclI T'GATC_A
Cuts at: 2583 2583
Size: 5063
BglI GCCn_nnn'nGGC
Cuts at: 244 253 565 244
Size: 9 312 4742
Fragments arranged by size:
4742 312 9
BglII A'GATC_T
Cuts at: 2578 4615 2578
Size: 2037 3026
BlpI GC'TnA_GC
Cuts at: 343 845 1200 2175 343
Size: 502 355 975 3231
Fragments arranged by size:
3231 975 502 355
Bme1580I G_kGCm'C
Cuts at: 170 358 875 994 1147 1456 1581 2688 2781
Size: 188 517 119 153 309 125 1107 93
Cuts at: 2781 3837 170
Size: 1056 1396
Fragments arranged by size:
1396 1107 1056 517 309 188 153 125
119 93
BmrI ACTGGGnnnn_n'
Cuts at: 1370 2275 2356 2691 4835 1370
Size: 905 81 335 2144 1598
Fragments arranged by size:
2144 1598 905 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1658 2007 3430 1658
Size: 349 1423 3291
Fragments arranged by size:
3291 1423 349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 769 1187 1199 1830 1903 261
Size: 508 418 12 631 73 3421
Fragments arranged by size:
3421 631 508 418 73 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2002 2239 3118 3610 3908 2002
Size: 237 879 492 298 3157
Fragments arranged by size:
3157 879 492 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 774 1646 1781 1917 1978 4767 774
Size: 872 135 136 61 2789 1070
Fragments arranged by size:
2789 1070 872 136 135 61
BsaAI yAC'GTr
Cuts at: 405 2416 405
Size: 2011 3052
BsaBI GATnn'nnATC
Cuts at: 6 694 2577 4614 6
Size: 688 1883 2037 455
Fragments arranged by size:
2037 1883 688 455
BsaHI Gr'CG_yC
Cuts at: 1592 1999 2494 2742 3444 4940 1592
Size: 407 495 248 702 1496 1715
Fragments arranged by size:
1715 1496 702 495 407 248
BsaWI w'CCGG_w
Cuts at: 700 2252 2773 3451 3725 3872 4283 700
Size: 1552 521 678 274 147 411 1480
Fragments arranged by size:
1552 1480 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1828 1858 1828
Size: 30 5033
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 174 922 1495 1677 1900 1927 1932 1935 1951
Size: 748 573 182 223 27 5 3 16
Cuts at: 1951 1954 174
Size: 3 3283
Fragments arranged by size:
3283 748 573 223 182 27 16 5
3 3
BseYI C'CCAG_C
Cuts at: 285 560 1066 1081 2488 3246 3823 4319 285
Size: 275 506 15 1407 758 577 496 1029
Fragments arranged by size:
1407 1029 758 577 506 496 275 15
BsiEI CG_ry'CG
Cuts at: 104 248 302 323 2605 2651 3859 104
Size: 144 54 21 2282 46 1208 1308
Fragments arranged by size:
2282 1308 1208 144 54 46 21
BsiHKAI G_wGCw'C
Cuts at: 170 358 815 851 1621 2077 2855 3045 3837
Size: 188 457 36 770 456 778 190 792
Cuts at: 3837 170
Size: 1396
Fragments arranged by size:
1396 792 778 770 457 456 190 188
36
BsmBI CGTCTCn'nnnn_
Cuts at: 1175 1175
Size: 5063
Bsp1286I G_dGCh'C
Cuts at: 170 275 292 358 815 851 875 994 1147
Size: 105 17 66 457 36 24 119 153
Cuts at: 1147 1456 1532 1581 1621 2077 2688 2781 2855
Size: 309 76 49 40 456 611 93 74
Cuts at: 2855 3045 3107 3837 170
Size: 190 62 730 1396
Fragments arranged by size:
1396 730 611 457 456 309 190 153
119 105 93 76 74 66 62 49
40 36 24 17
BspEI T'CCGG_A
Cuts at: 4283 4283
Size: 5063
BspHI T'CATG_A
Cuts at: 4239 4239
Size: 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1628 2113 2327 2629 3010 1628
Size: 485 214 302 381 3681
Fragments arranged by size:
3681 485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1392 1583 3355 3409 4893 5058 50
Size: 1342 191 1772 54 1484 165 55
Fragments arranged by size:
1772 1484 1342 191 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2975 2975
Size: 5063
BsrFI r'CCGG_y
Cuts at: 265 1039 3061 3242 3451 265
Size: 774 2022 181 209 1877
Fragments arranged by size:
2022 1877 774 209 181
BssHII G'CGCG_C
Cuts at: 188 188
Size: 5063
BssSI C'ACGA_G
Cuts at: 1420 1647 3334 3692 1420
Size: 227 1687 358 2791
Fragments arranged by size:
2791 1687 358 227
BstAPI GCAn_nnn'nTGC
Cuts at: 353 2440 353
Size: 2087 2976
BstBI TT'CG_AA
Cuts at: 3424 3424
Size: 5063
BstEII G'GTnAC_C
Cuts at: 381 381
Size: 5063
BstXI CCAn_nnnn'nTGG
Cuts at: 285 285
Size: 5063
BstYI r'GATC_y
Cuts at: 1 2088 2555 2578 2913 3159 4171 4257 4269
Size: 2087 467 23 335 246 1012 86 12
Cuts at: 4269 4615 1
Size: 346 449
Fragments arranged by size:
2087 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 202 834 1056 202
Size: 632 222 4209
Fragments arranged by size:
4209 632 222
BtgI C'CryG_G
Cuts at: 764 1139 1586 1687 764
Size: 375 447 101 4140
Fragments arranged by size:
4140 447 375 101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 265 498 3013 3194 3357 265
Size: 233 2515 181 163 1971
Fragments arranged by size:
2515 1971 233 181 163
BtsI GCAGTG_nn'
Cuts at: 1257 1278 2306 1257
Size: 21 1028 4014
Fragments arranged by size:
4014 1028 21
DraI TTT'AAA
Cuts at: 2062 2062
Size: 5063
DraIII CAC_nnn'GTG
Cuts at: 353 408 353
Size: 55 5008
DrdI GACnn_nn'nnGTC
Cuts at: 322 2769 3627 322
Size: 2447 858 1758
Fragments arranged by size:
2447 1758 858
EaeI y'GGCC_r
Cuts at: 245 263 299 533 635 1209 1343 1380 1491
Size: 18 36 234 102 574 134 37 111
Cuts at: 1491 2648 2822 3213 3240 4585 245
Size: 1157 174 391 27 1345 723
Fragments arranged by size:
1345 1157 723 574 391 234 174 134
111 102 37 36 27 18
EagI C'GGCC_G
Cuts at: 245 299 2648 245
Size: 54 2349 2660
Fragments arranged by size:
2660 2349 54
EarI CTCTTCn'nnn_
Cuts at: 421 895 1940 3086 3296 421
Size: 474 1045 1146 210 2188
Fragments arranged by size:
2188 1146 1045 474 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3271 3581 3727 4873 3271
Size: 310 146 1146 3461
Fragments arranged by size:
3461 1146 310 146
EcoICRI GAG'CTC
Cuts at: 813 849 2075 813
Size: 36 1226 3801
Fragments arranged by size:
3801 1226 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 261 769 1187 1199 1283 1755 1830 1903 2888
Size: 508 418 12 84 472 75 73 985
Cuts at: 2888 3320 4067 4335 261
Size: 432 747 268 989
Fragments arranged by size:
989 985 747 508 472 432 418 268
84 75 73 12
EcoNI CCTnn'n_nnAGG
Cuts at: 1420 1420
Size: 5063
EcoO109I rG'GnC_Cy
Cuts at: 780 830 871 1026 1144 1317 1452 1453 1752
Size: 50 41 155 118 173 135 1 299
Cuts at: 1752 1805 1827 1857 2202 780
Size: 53 22 30 345 3641
Fragments arranged by size:
3641 345 299 173 155 135 118 53
50 41 30 22 1
EcoRI G'AATT_C
Cuts at: 2067 2067
Size: 5063
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2043 3082 2043
Size: 1039 4024
FspI TGC'GCA
Cuts at: 619 2844 619
Size: 2225 2838
FspAI rTGC'GCAy
Cuts at: 619 619
Size: 5063
HaeII r_GCGC'y
Cuts at: 259 1788 2497 2745 3767 4746 259
Size: 1529 709 248 1022 979 576
Fragments arranged by size:
1529 1022 979 709 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 511 543 1420 1452 1759 1789 1791 1821 1828
Size: 32 877 32 307 30 2 30 7
Cuts at: 1828 1860 2905 2935 2937 2967 511
Size: 32 1045 30 2 30 2607
Fragments arranged by size:
2607 1045 877 307 32 32 32 30
30 30 30 7 2 2
HincII GTy'rAC
Cuts at: 543 1188 2102 543
Size: 645 914 3504
Fragments arranged by size:
3504 914 645
KasI G'GCGC_C
Cuts at: 2493 2741 2493
Size: 248 4815
KpnI G_GTAC'C
Cuts at: 1164 2083 1164
Size: 919 4144
MluI A'CGCG_T
Cuts at: 4496 4496
Size: 5063
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 1889 3734 3918 416
Size: 1473 1845 184 1561
Fragments arranged by size:
1845 1561 1473 184
MscI TGG'CCA
Cuts at: 637 1211 1345 1493 2824 637
Size: 574 134 148 1331 2876
Fragments arranged by size:
2876 1331 574 148 134
MslI CAynn'nnrTG
Cuts at: 379 3052 3179 379
Size: 2673 127 2263
Fragments arranged by size:
2673 2263 127
MspA1I CmG'CkG
Cuts at: 564 1204 1689 2174 2488 2848 3861 4106 564
Size: 640 485 485 314 360 1013 245 1521
Fragments arranged by size:
1521 1013 640 485 485 360 314 245
NaeI GCC'GGC
Cuts at: 267 1041 3244 267
Size: 774 2203 2086
Fragments arranged by size:
2203 2086 774
NarI GG'CG_CC
Cuts at: 2494 2742 2494
Size: 248 4815
NcoI C'CATG_G
Cuts at: 1139 1139
Size: 5063
NgoMIV G'CCGG_C
Cuts at: 265 1039 3242 265
Size: 774 2203 2086
Fragments arranged by size:
2203 2086 774
NspI r_CATG'y
Cuts at: 1683 2116 2616 3523 1683
Size: 433 500 907 3223
Fragments arranged by size:
3223 907 500 433
PasI CC'CwG_GG
Cuts at: 663 1511 663
Size: 848 4215
PciI A'CATG_T
Cuts at: 3519 3519
Size: 5063
PflMI CCAn_nnn'nTGG
Cuts at: 1517 1517
Size: 5063
PfoI T'CCnGG_A
Cuts at: 1472 1472
Size: 5063
PmeI GTTT'AAAC
Cuts at: 2062 2062
Size: 5063
PmlI CAC'GTG
Cuts at: 405 405
Size: 5063
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 4202
Size: 32 5031
PpuMI rG'GwC_Cy
Cuts at: 780 830 1317 1752 1805 1827 1857 780
Size: 50 487 435 53 22 30 3986
Fragments arranged by size:
3986 487 435 53 50 30 22
PspOMI G'GGCC_C
Cuts at: 871 1143 1452 1577 871
Size: 272 309 125 4357
Fragments arranged by size:
4357 309 272 125
PspXI vC'TCGA_Gb
Cuts at: 1728 1728
Size: 5063
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 568 600 568
Size: 32 5031
PstI C_TGCA'G
Cuts at: 2110 2795 2110
Size: 685 4378
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5063
PvuII CAG'CTG
Cuts at: 564 1204 2488 2848 564
Size: 640 1284 360 2779
Fragments arranged by size:
2779 1284 640 360
RsrII CG'GwC_CG
Cuts at: 3258 3258
Size: 5063
SacI G_AGCT'C
Cuts at: 815 851 2077 815
Size: 36 1226 3801
Fragments arranged by size:
3801 1226 36
SacII CC_GC'GG
Cuts at: 1690 1690
Size: 5063
SalI G'TCGA_C
Cuts at: 541 2100 541
Size: 1559 3504
SanDI GG'GwC_CC
Cuts at: 1752 1827 1752
Size: 75 4988
SapI GCTCTTCn'nnn_
Cuts at: 895 3086 3296 895
Size: 2191 210 2662
Fragments arranged by size:
2662 2191 210
SbfI CC_TGCA'GG
Cuts at: 2110 2110
Size: 5063
ScaI AGT'ACT
Cuts at: 4580 4580
Size: 5063
SexAI A'CCwGG_T
Cuts at: 349 855 349
Size: 506 4557
SfcI C'TryA_G
Cuts at: 33 1278 2106 2791 3784 3975 33
Size: 1245 828 685 993 191 1121
Fragments arranged by size:
1245 1121 993 828 685 191
SfoI GGC'GCC
Cuts at: 2495 2743 2495
Size: 248 4815
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5063
SmaI CCC'GGG
Cuts at: 1450 1576 1750 1825 2085 1450
Size: 126 174 75 260 4428
Fragments arranged by size:
4428 260 174 126 75
SmlI C'TyrA_G
Cuts at: 1728 2017 2218 3133 3625 3887 1728
Size: 289 201 915 492 262 2904
Fragments arranged by size:
2904 915 492 289 262 201
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5063
SphI G_CATG'C
Cuts at: 2116 2616 2116
Size: 500 4563
StuI AGG'CCT
Cuts at: 802 1951 802
Size: 1149 3914
StyI C'CwwG_G
Cuts at: 181 822 1139 1212 2197 2551 181
Size: 641 317 73 985 354 2693
Fragments arranged by size:
2693 985 641 354 317 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2002 2646 2779 3238 3447 2002
Size: 644 133 459 209 3618
Fragments arranged by size:
3618 644 459 209 133
TatI w'GTAC_w
Cuts at: 488 1322 4578 488
Size: 834 3256 973
Fragments arranged by size:
3256 973 834
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 132 164 1468 1500 4580 4612 132
Size: 32 1304 32 3080 32 583
Fragments arranged by size:
3080 1304 583 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 319 387 2860 319
Size: 68 2473 2522
Fragments arranged by size:
2522 2473 68
XbaI T'CTAG_A
Cuts at: 2094 2094
Size: 5063
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1383 2558 1383
Size: 1175 3888
XhoI C'TCGA_G
Cuts at: 1728 1728
Size: 5063
XmaI C'CCGG_G
Cuts at: 1448 1574 1748 1823 2083 1448
Size: 126 174 75 260 4428
Fragments arranged by size:
4428 260 174 126 75
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI
BsmI BsrGI BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PshAI PsiI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI