Restriction map for
pF1KB6171
(Circular) (Six-base) MAPSORT of: pF1KB6171.seq Check: 6434 from: 1 to: 5060
pF1KB6171 5060 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1946 1946
Size: 5060
AccI GT'mk_AC
Cuts at: 2098 2098
Size: 5060
Acc65I G'GTAC_C
Cuts at: 2076 2076
Size: 5060
AclI AA'CG_TT
Cuts at: 4900 4900
Size: 5060
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 747 2885 3317 4064 4332 502
Size: 245 2138 432 747 268 1230
Fragments arranged by size:
2138 1230 747 432 268 245
AflIII A'CryG_T
Cuts at: 2412 3516 4493 2412
Size: 1104 977 2979
Fragments arranged by size:
2979 1104 977
AgeI A'CCGG_T
Cuts at: 3448 3448
Size: 5060
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1633 1665 1633
Size: 32 5028
AlwNI CAG_nnn'CTG
Cuts at: 2043 2365 3932 2043
Size: 322 1567 3171
Fragments arranged by size:
3171 1567 322
ApaI G_GGCC'C
Cuts at: 166 166
Size: 5060
ApaLI G'TGCA_C
Cuts at: 1221 3830 1221
Size: 2609 2451
ApoI r'AATT_y
Cuts at: 918 2064 4413 4669 918
Size: 1146 2349 256 1309
Fragments arranged by size:
2349 1309 1146 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5060
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
AvaI C'yCGr_G
Cuts at: 768 2080 768
Size: 1312 3748
AvrII C'CTAG_G
Cuts at: 1950 1950
Size: 5060
BamHI G'GATC_C
Cuts at: 2085 2085
Size: 5060
BanI G'GyrC_C
Cuts at: 111 171 828 2076 2490 2738 2773 111
Size: 60 657 1248 414 248 35 2398
Fragments arranged by size:
2398 1248 657 414 248 60 35
BanII G_rGCy'C
Cuts at: 166 1211 1666 2054 2074 3104 166
Size: 1045 455 388 20 1030 2122
Fragments arranged by size:
2122 1045 1030 455 388 20
BbeI G_GCGC'C
Cuts at: 175 2494 2742 175
Size: 2319 248 2493
Fragments arranged by size:
2493 2319 248
BbsI GAAGACnn'nnnn_
Cuts at: 324 1755 324
Size: 1431 3629
BbvCI CC'TCA_GC
Cuts at: 866 866
Size: 5060
BciVI GTATCCnnnnn_n'
Cuts at: 1127 2958 3719 5060 1127
Size: 1831 761 1341 1127
Fragments arranged by size:
1831 1341 1127 761
BclI T'GATC_A
Cuts at: 597 1094 2580 597
Size: 497 1486 3077
Fragments arranged by size:
3077 1486 497
BfrBI ATG'CAT
Cuts at: 945 945
Size: 5060
BglI GCCn_nnn'nGGC
Cuts at: 1712 1874 1712
Size: 162 4898
BglII A'GATC_T
Cuts at: 1283 2575 4612 1283
Size: 1292 2037 1731
Fragments arranged by size:
2037 1731 1292
BlpI GC'TnA_GC
Cuts at: 2172 2172
Size: 5060
Bme1580I G_kGCm'C
Cuts at: 166 1225 1338 1967 2685 2778 3834 166
Size: 1059 113 629 718 93 1056 1392
Fragments arranged by size:
1392 1059 1056 718 629 113 93
BmgBI CAC'GTC
Cuts at: 1242 1242
Size: 5060
BmrI ACTGGGnnnn_n'
Cuts at: 1332 2272 2353 2688 4832 1332
Size: 940 81 335 2144 1560
Fragments arranged by size:
2144 1560 940 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 5060
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 856 1103 1264 1928 856
Size: 247 161 664 3988
Fragments arranged by size:
3988 664 247 161
Bpu10I CC'TnA_GC
Cuts at: 866 1088 866
Size: 222 4838
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 3115 3607 3905 2236
Size: 879 492 298 3391
Fragments arranged by size:
3391 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 460 976 4764 460
Size: 516 3788 756
Fragments arranged by size:
3788 756 516
BsaAI yAC'GTr
Cuts at: 2413 2413
Size: 5060
BsaBI GATnn'nnATC
Cuts at: 6 1115 2574 4611 6
Size: 1109 1459 2037 455
Fragments arranged by size:
2037 1459 1109 455
BsaHI Gr'CG_yC
Cuts at: 172 2491 2739 3441 4937 172
Size: 2319 248 702 1496 295
Fragments arranged by size:
2319 1496 702 295 248
BsaWI w'CCGG_w
Cuts at: 374 575 2249 2770 3448 3722 3869 4280 374
Size: 201 1674 521 678 274 147 411 1154
Fragments arranged by size:
1674 1154 678 521 411 274 201 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1076 1106 1528 1558 1076
Size: 30 422 30 4578
Fragments arranged by size:
4578 422 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 259 316 883 259
Size: 57 567 4436
Fragments arranged by size:
4436 567 57
BseYI C'CCAG_C
Cuts at: 1446 1665 1710 2485 3243 3820 4316 1446
Size: 219 45 775 758 577 496 2190
Fragments arranged by size:
2190 775 758 577 496 219 45
BsiEI CG_ry'CG
Cuts at: 104 255 2602 2648 3856 104
Size: 151 2347 46 1208 1308
Fragments arranged by size:
2347 1308 1208 151 46
BsiHKAI G_wGCw'C
Cuts at: 730 1225 2054 2074 2852 3042 3834 730
Size: 495 829 20 778 190 792 1956
Fragments arranged by size:
1956 829 792 778 495 190 20
BsmI GAATG_Cn'
Cuts at: 1181 1181
Size: 5060
BsmBI CGTCTCn'nnnn_
Cuts at: 549 549
Size: 5060
Bsp1286I G_dGCh'C
Cuts at: 166 730 1211 1225 1338 1666 1967 2054 2074
Size: 564 481 14 113 328 301 87 20
Cuts at: 2074 2685 2778 2852 3042 3104 3834 166
Size: 611 93 74 190 62 730 1392
Fragments arranged by size:
1392 730 611 564 481 328 301 190
113 93 87 74 62 20 14
BspEI T'CCGG_A
Cuts at: 374 4280 374
Size: 3906 1154
BspHI T'CATG_A
Cuts at: 4236 4236
Size: 5060
BspMI ACCTGCnnnn'nnnn_
Cuts at: 892 1946 2110 2324 2626 3007 892
Size: 1054 164 214 302 381 2945
Fragments arranged by size:
2945 1054 381 302 214 164
BsrBI CCG'CTC
Cuts at: 50 3352 3406 4890 5055 50
Size: 3302 54 1484 165 55
Fragments arranged by size:
3302 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1163 1787 2972 1163
Size: 624 1185 3251
Fragments arranged by size:
3251 1185 624
BsrFI r'CCGG_y
Cuts at: 1574 3058 3239 3448 1574
Size: 1484 181 209 3186
Fragments arranged by size:
3186 1484 209 181
BsrGI T'GTAC_A
Cuts at: 1037 1037
Size: 5060
BssSI C'ACGA_G
Cuts at: 1069 3331 3689 1069
Size: 2262 358 2440
Fragments arranged by size:
2440 2262 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2437 2437
Size: 5060
BstBI TT'CG_AA
Cuts at: 3421 3421
Size: 5060
BstEII G'GTnAC_C
Cuts at: 879 1161 879
Size: 282 4778
BstXI CCAn_nnnn'nTGG
Cuts at: 708 1509 708
Size: 801 4259
BstYI r'GATC_y
Cuts at: 1 1283 1648 2085 2552 2575 2910 3156 4168
Size: 1282 365 437 467 23 335 246 1012
Cuts at: 4168 4254 4266 4612 1
Size: 86 12 346 449
Fragments arranged by size:
1282 1012 467 449 437 365 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 167 167
Size: 5060
BtgI C'CryG_G
Cuts at: 107 222 249 296 1001 107
Size: 115 27 47 705 4166
Fragments arranged by size:
4166 705 115 47 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3010 3191 3354 3010
Size: 181 163 4716
Fragments arranged by size:
4716 181 163
BtsI GCAGTG_nn'
Cuts at: 322 2303 322
Size: 1981 3079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1298 1333 1683 1718 1298
Size: 35 350 35 4640
Fragments arranged by size:
4640 350 35 35
DraI TTT'AAA
Cuts at: 1485 2059 1485
Size: 574 4486
DrdI GACnn_nn'nnGTC
Cuts at: 2766 3624 2766
Size: 858 4202
EaeI y'GGCC_r
Cuts at: 252 1519 1610 1704 2645 2819 3210 3237 4582
Size: 1267 91 94 941 174 391 27 1345
Cuts at: 4582 252
Size: 730
Fragments arranged by size:
1345 1267 941 730 391 174 94 91
27
EagI C'GGCC_G
Cuts at: 252 2645 252
Size: 2393 2667
EarI CTCTTCn'nnn_
Cuts at: 1043 1436 3083 3293 1043
Size: 393 1647 210 2810
Fragments arranged by size:
2810 1647 393 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 541 3268 3578 3724 4870 541
Size: 2727 310 146 1146 731
Fragments arranged by size:
2727 1146 731 310 146
EcoICRI GAG'CTC
Cuts at: 2052 2072 2052
Size: 20 5040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 747 856 1103 1264 1928 2885 3317 4064
Size: 245 109 247 161 664 957 432 747
Cuts at: 4064 4332 502
Size: 268 1230
Fragments arranged by size:
1230 957 747 664 432 268 247 245
161 109
EcoO109I rG'GnC_Cy
Cuts at: 163 477 2009 2199 163
Size: 314 1532 190 3024
Fragments arranged by size:
3024 1532 314 190
EcoRI G'AATT_C
Cuts at: 2064 2064
Size: 5060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1623 3079 1623
Size: 1456 3604
FspI TGC'GCA
Cuts at: 2841 2841
Size: 5060
HaeII r_GCGC'y
Cuts at: 175 519 2494 2742 3764 4743 175
Size: 344 1975 248 1022 979 492
Fragments arranged by size:
1975 1022 979 492 344 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 110 142 150 182 514 546 582 614 886
Size: 32 8 32 332 32 36 32 272
Cuts at: 886 918 1086 1118 1418 1450 1765 1797 2902
Size: 32 168 32 300 32 315 32 1105
Cuts at: 2902 2932 2934 2964 110
Size: 30 2 30 2206
Fragments arranged by size:
2206 1105 332 315 300 272 168 36
32 32 32 32 32 32 32 32
30 30 8 2
HincII GTy'rAC
Cuts at: 2099 2099
Size: 5060
KasI G'GCGC_C
Cuts at: 171 2490 2738 171
Size: 2319 248 2493
Fragments arranged by size:
2493 2319 248
KpnI G_GTAC'C
Cuts at: 2080 2080
Size: 5060
MluI A'CGCG_T
Cuts at: 4493 4493
Size: 5060
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 744 767 1718 2042 3731 3915 744
Size: 23 951 324 1689 184 1889
Fragments arranged by size:
1889 1689 951 324 184 23
MscI TGG'CCA
Cuts at: 1521 1612 1706 2821 1521
Size: 91 94 1115 3760
Fragments arranged by size:
3760 1115 94 91
MslI CAynn'nnrTG
Cuts at: 241 1000 3049 3176 241
Size: 759 2049 127 2125
Fragments arranged by size:
2125 2049 759 127
MspA1I CmG'CkG
Cuts at: 251 585 1710 2171 2485 2845 3858 4103 251
Size: 334 1125 461 314 360 1013 245 1208
Fragments arranged by size:
1208 1125 1013 461 360 334 314 245
NaeI GCC'GGC
Cuts at: 1576 3241 1576
Size: 1665 3395
NarI GG'CG_CC
Cuts at: 172 2491 2739 172
Size: 2319 248 2493
Fragments arranged by size:
2493 2319 248
NcoI C'CATG_G
Cuts at: 107 222 1001 107
Size: 115 779 4166
Fragments arranged by size:
4166 779 115
NgoMIV G'CCGG_C
Cuts at: 1574 3239 1574
Size: 1665 3395
NsiI A_TGCA'T
Cuts at: 947 947
Size: 5060
NspI r_CATG'y
Cuts at: 2113 2613 3520 2113
Size: 500 907 3653
Fragments arranged by size:
3653 907 500
PciI A'CATG_T
Cuts at: 3516 3516
Size: 5060
PflMI CCAn_nnn'nTGG
Cuts at: 1820 2043 1820
Size: 223 4837
PfoI T'CCnGG_A
Cuts at: 508 532 1436 508
Size: 24 904 4132
Fragments arranged by size:
4132 904 24
PmeI GTTT'AAAC
Cuts at: 2059 2059
Size: 5060
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 561 4199 4231 529
Size: 32 3638 32 1358
Fragments arranged by size:
3638 1358 32 32
PspOMI G'GGCC_C
Cuts at: 162 162
Size: 5060
PstI C_TGCA'G
Cuts at: 396 1365 1480 2107 2792 396
Size: 969 115 627 685 2664
Fragments arranged by size:
2664 969 685 627 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5060
PvuII CAG'CTG
Cuts at: 1710 2485 2845 1710
Size: 775 360 3925
Fragments arranged by size:
3925 775 360
RsrII CG'GwC_CG
Cuts at: 3255 3255
Size: 5060
SacI G_AGCT'C
Cuts at: 2054 2074 2054
Size: 20 5040
SacII CC_GC'GG
Cuts at: 252 252
Size: 5060
SalI G'TCGA_C
Cuts at: 2097 2097
Size: 5060
SapI GCTCTTCn'nnn_
Cuts at: 1436 3083 3293 1436
Size: 1647 210 3203
Fragments arranged by size:
3203 1647 210
SbfI CC_TGCA'GG
Cuts at: 2107 2107
Size: 5060
ScaI AGT'ACT
Cuts at: 4577 4577
Size: 5060
SfcI C'TryA_G
Cuts at: 33 392 1169 1212 1361 1476 2103 2788 3781
Size: 359 777 43 149 115 627 685 993
Cuts at: 3781 3972 33
Size: 191 1121
Fragments arranged by size:
1121 993 777 685 627 359 191 149
115 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 1712 1712
Size: 5060
SfoI GGC'GCC
Cuts at: 173 2492 2740 173
Size: 2319 248 2493
Fragments arranged by size:
2493 2319 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5060
SmaI CCC'GGG
Cuts at: 2082 2082
Size: 5060
SmlI C'TyrA_G
Cuts at: 2215 3130 3622 3884 2215
Size: 915 492 262 3391
Fragments arranged by size:
3391 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5060
SphI G_CATG'C
Cuts at: 2113 2613 2113
Size: 500 4560
StyI C'CwwG_G
Cuts at: 107 222 1001 1569 1814 1950 2194 2548 107
Size: 115 779 568 245 136 244 354 2619
Fragments arranged by size:
2619 779 568 354 245 244 136 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 1319 2643 2776 3235 3444 235
Size: 1084 1324 133 459 209 1851
Fragments arranged by size:
1851 1324 1084 459 209 133
TatI w'GTAC_w
Cuts at: 425 1037 1340 4575 425
Size: 612 303 3235 910
Fragments arranged by size:
3235 910 612 303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 682 714 1076 1108 1946 1978 4577 4609 682
Size: 32 362 32 838 32 2599 32 1133
Fragments arranged by size:
2599 1133 838 362 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1188 2857 1188
Size: 1669 3391
XbaI T'CTAG_A
Cuts at: 2091 2091
Size: 5060
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 943 2555 708
Size: 235 1612 3213
Fragments arranged by size:
3213 1612 235
XmaI C'CCGG_G
Cuts at: 2080 2080
Size: 5060
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI BaeI
BcgI BmtI BsgI BsiWI BssHII BstZ17I ClaI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI