Restriction map for
pF1KB6173
(Circular) (Six-base) MAPSORT of: pF1KB6173.seq Check: 8560 from: 1 to: 5072
pF1KB6173 5072 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 889 889
Size: 5072
AccI GT'mk_AC
Cuts at: 2110 2110
Size: 5072
Acc65I G'GTAC_C
Cuts at: 2088 2088
Size: 5072
AclI AA'CG_TT
Cuts at: 4912 4912
Size: 5072
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 527 978 1554 2897 3329 4076 4344 527
Size: 451 576 1343 432 747 268 1255
Fragments arranged by size:
1343 1255 747 576 451 432 268
AflIII A'CryG_T
Cuts at: 1500 2424 3528 4505 1500
Size: 924 1104 977 2067
Fragments arranged by size:
2067 1104 977 924
AgeI A'CCGG_T
Cuts at: 3460 3460
Size: 5072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 941 951 941
Size: 10 5062
AlwNI CAG_nnn'CTG
Cuts at: 708 1851 2377 3944 708
Size: 1143 526 1567 1836
Fragments arranged by size:
1836 1567 1143 526
ApaI G_GGCC'C
Cuts at: 1024 1024
Size: 5072
ApaLI G'TGCA_C
Cuts at: 740 3842 740
Size: 3102 1970
ApoI r'AATT_y
Cuts at: 341 1125 1436 1508 1604 2076 4425 4681 341
Size: 784 311 72 96 472 2349 256 732
Fragments arranged by size:
2349 784 732 472 311 256 96 72
AseI AT'TA_AT
Cuts at: 20 20
Size: 5072
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
AvaI C'yCGr_G
Cuts at: 632 889 2092 632
Size: 257 1203 3612
Fragments arranged by size:
3612 1203 257
AvrII C'CTAG_G
Cuts at: 735 735
Size: 5072
BamHI G'GATC_C
Cuts at: 396 2097 396
Size: 1701 3371
BanI G'GyrC_C
Cuts at: 874 1070 2015 2088 2502 2750 2785 874
Size: 196 945 73 414 248 35 3161
Fragments arranged by size:
3161 945 414 248 196 73 35
BanII G_rGCy'C
Cuts at: 377 1024 1750 1918 2002 2086 3116 377
Size: 647 726 168 84 84 1030 2333
Fragments arranged by size:
2333 1030 726 647 168 84 84
BbeI G_GCGC'C
Cuts at: 2506 2754 2506
Size: 248 4824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 880 914 880
Size: 34 5038
BciVI GTATCCnnnnn_n'
Cuts at: 377 2970 3731 5072 377
Size: 2593 761 1341 377
Fragments arranged by size:
2593 1341 761 377
BclI T'GATC_A
Cuts at: 2592 2592
Size: 5072
BfrBI ATG'CAT
Cuts at: 1966 1966
Size: 5072
BglII A'GATC_T
Cuts at: 2587 4624 2587
Size: 2037 3035
BlpI GC'TnA_GC
Cuts at: 1833 1871 2184 1833
Size: 38 313 4721
Fragments arranged by size:
4721 313 38
Bme1580I G_kGCm'C
Cuts at: 744 877 1024 1073 2018 2697 2790 3846 744
Size: 133 147 49 945 679 93 1056 1970
Fragments arranged by size:
1970 1056 945 679 147 133 93 49
BmrI ACTGGGnnnn_n'
Cuts at: 178 530 934 2284 2365 2700 4844 178
Size: 352 404 1350 81 335 2144 406
Fragments arranged by size:
2144 1350 406 404 352 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3439 3439
Size: 5072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 259 361 730 745 1078 1348 1802 99
Size: 160 102 369 15 333 270 454 3369
Fragments arranged by size:
3369 454 369 333 270 160 102 15
Bpu10I CC'TnA_GC
Cuts at: 371 1274 371
Size: 903 4169
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 1164 2248 3127 3619 3917 332
Size: 832 1084 879 492 298 1487
Fragments arranged by size:
1487 1084 879 832 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 1054 1083 1782 4776 264
Size: 790 29 699 2994 560
Fragments arranged by size:
2994 790 699 560 29
BsaAI yAC'GTr
Cuts at: 1501 2425 1501
Size: 924 4148
BsaBI GATnn'nnATC
Cuts at: 6 2586 4623 6
Size: 2580 2037 455
Fragments arranged by size:
2580 2037 455
BsaHI Gr'CG_yC
Cuts at: 2503 2751 3453 4949 2503
Size: 248 702 1496 2626
Fragments arranged by size:
2626 1496 702 248
BsaWI w'CCGG_w
Cuts at: 758 1655 1696 2261 2782 3460 3734 3881 4292
Size: 897 41 565 521 678 274 147 411
Cuts at: 4292 758
Size: 1538
Fragments arranged by size:
1538 897 678 565 521 411 274 147
41
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 853 925 973 1018 322
Size: 531 72 48 45 4376
Fragments arranged by size:
4376 531 72 48 45
BseYI C'CCAG_C
Cuts at: 902 1017 1156 1844 2497 3255 3832 4328 902
Size: 115 139 688 653 758 577 496 1646
Fragments arranged by size:
1646 758 688 653 577 496 139 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 545 545
Size: 5072
BsiEI CG_ry'CG
Cuts at: 104 2614 2660 3868 104
Size: 2510 46 1208 1308
Fragments arranged by size:
2510 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 377 744 953 1341 1750 1918 2002 2086 2864
Size: 367 209 388 409 168 84 84 778
Cuts at: 2864 3054 3846 377
Size: 190 792 1603
Fragments arranged by size:
1603 792 778 409 388 367 209 190
168 84 84
BsmI GAATG_Cn'
Cuts at: 486 486
Size: 5072
Bsp1286I G_dGCh'C
Cuts at: 377 744 877 953 1024 1073 1341 1750 1918
Size: 367 133 76 71 49 268 409 168
Cuts at: 1918 2002 2018 2086 2697 2790 2864 3054 3116
Size: 84 16 68 611 93 74 190 62
Cuts at: 3116 3846 377
Size: 730 1603
Fragments arranged by size:
1603 730 611 409 367 268 190 168
133 93 84 76 74 71 68 62
49 16
BspEI T'CCGG_A
Cuts at: 758 1655 4292 758
Size: 897 2637 1538
Fragments arranged by size:
2637 1538 897
BspHI T'CATG_A
Cuts at: 4248 4248
Size: 5072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 534 873 889 2122 2336 2638 3019 534
Size: 339 16 1233 214 302 381 2587
Fragments arranged by size:
2587 1233 381 339 302 214 16
BsrBI CCG'CTC
Cuts at: 50 3364 3418 4902 5067 50
Size: 3314 54 1484 165 55
Fragments arranged by size:
3314 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1557 2984 1557
Size: 1427 3645
BsrFI r'CCGG_y
Cuts at: 3070 3251 3460 3070
Size: 181 209 4682
Fragments arranged by size:
4682 209 181
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 5072
BssHII G'CGCG_C
Cuts at: 586 586
Size: 5072
BssSI C'ACGA_G
Cuts at: 3343 3701 3343
Size: 358 4714
BstAPI GCAn_nnn'nTGC
Cuts at: 881 2449 881
Size: 1568 3504
BstBI TT'CG_AA
Cuts at: 3433 3433
Size: 5072
BstEII G'GTnAC_C
Cuts at: 571 571
Size: 5072
BstYI r'GATC_y
Cuts at: 1 396 488 704 2097 2564 2587 2922 3168
Size: 395 92 216 1393 467 23 335 246
Cuts at: 3168 4180 4266 4278 4624 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1393 1012 467 449 395 346 335 246
216 92 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 578 683 956 1139 1852 578
Size: 105 273 183 713 3798
Fragments arranged by size:
3798 713 273 183 105
BtgI C'CryG_G
Cuts at: 107 107
Size: 5072
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1061 3022 3203 3366 1061
Size: 1961 181 163 2767
Fragments arranged by size:
2767 1961 181 163
BtsI GCAGTG_nn'
Cuts at: 742 896 1641 2315 742
Size: 154 745 674 3499
Fragments arranged by size:
3499 745 674 154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1959 1994 1959
Size: 35 5037
DraI TTT'AAA
Cuts at: 2071 2071
Size: 5072
DrdI GACnn_nn'nnGTC
Cuts at: 132 831 2778 3636 132
Size: 699 1947 858 1568
Fragments arranged by size:
1947 1568 858 699
EaeI y'GGCC_r
Cuts at: 325 2657 2831 3222 3249 4594 325
Size: 2332 174 391 27 1345 803
Fragments arranged by size:
2332 1345 803 391 174 27
EagI C'GGCC_G
Cuts at: 2657 2657
Size: 5072
EarI CTCTTCn'nnn_
Cuts at: 179 407 1513 1755 3095 3305 179
Size: 228 1106 242 1340 210 1946
Fragments arranged by size:
1946 1340 1106 242 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1897 3280 3590 3736 4882 1897
Size: 1383 310 146 1146 2087
Fragments arranged by size:
2087 1383 1146 310 146
EcoICRI GAG'CTC
Cuts at: 375 1748 1916 2000 2084 375
Size: 1373 168 84 84 3363
Fragments arranged by size:
3363 1373 168 84 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 99 259 361 527 730 745 978 1078 1348
Size: 160 102 166 203 15 233 100 270
Cuts at: 1348 1554 1802 2897 3329 4076 4344 99
Size: 206 248 1095 432 747 268 827
Fragments arranged by size:
1095 827 747 432 270 268 248 233
206 203 166 160 102 100 15
EcoNI CCTnn'n_nnAGG
Cuts at: 814 814
Size: 5072
EcoO109I rG'GnC_Cy
Cuts at: 444 495 628 639 1706 1817 2211 444
Size: 51 133 11 1067 111 394 3305
Fragments arranged by size:
3305 1067 394 133 111 51 11
EcoRI G'AATT_C
Cuts at: 341 1604 2076 341
Size: 1263 472 3337
Fragments arranged by size:
3337 1263 472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1320 3091 1320
Size: 1771 3301
FspI TGC'GCA
Cuts at: 1459 2853 1459
Size: 1394 3678
HaeII r_GCGC'y
Cuts at: 1406 1871 1959 2506 2754 3776 4755 1406
Size: 465 88 547 248 1022 979 1723
Fragments arranged by size:
1723 1022 979 547 465 248 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 280 312 2914 2944 2946 2976 280
Size: 32 2602 30 2 30 2376
Fragments arranged by size:
2602 2376 32 30 30 2
HincII GTy'rAC
Cuts at: 2111 2111
Size: 5072
KasI G'GCGC_C
Cuts at: 2502 2750 2502
Size: 248 4824
KpnI G_GTAC'C
Cuts at: 2092 2092
Size: 5072
MluI A'CGCG_T
Cuts at: 4505 4505
Size: 5072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 148 573 1555 3743 3927 148
Size: 425 982 2188 184 1293
Fragments arranged by size:
2188 1293 982 425 184
MscI TGG'CCA
Cuts at: 327 2833 327
Size: 2506 2566
MslI CAynn'nnrTG
Cuts at: 569 1797 1965 3061 3188 569
Size: 1228 168 1096 127 2453
Fragments arranged by size:
2453 1228 1096 168 127
MspA1I CmG'CkG
Cuts at: 381 2183 2497 2857 3870 4115 381
Size: 1802 314 360 1013 245 1338
Fragments arranged by size:
1802 1338 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3253 3253
Size: 5072
NarI GG'CG_CC
Cuts at: 2503 2751 2503
Size: 248 4824
NcoI C'CATG_G
Cuts at: 107 107
Size: 5072
NdeI CA'TA_TG
Cuts at: 1962 1962
Size: 5072
NgoMIV G'CCGG_C
Cuts at: 3251 3251
Size: 5072
NsiI A_TGCA'T
Cuts at: 1968 1968
Size: 5072
NspI r_CATG'y
Cuts at: 1970 2125 2625 3532 1970
Size: 155 500 907 3510
Fragments arranged by size:
3510 907 500 155
PciI A'CATG_T
Cuts at: 3528 3528
Size: 5072
PflMI CCAn_nnn'nTGG
Cuts at: 465 801 981 465
Size: 336 180 4556
Fragments arranged by size:
4556 336 180
PfoI T'CCnGG_A
Cuts at: 707 974 1232 707
Size: 267 258 4547
Fragments arranged by size:
4547 267 258
PmeI GTTT'AAAC
Cuts at: 2071 2071
Size: 5072
PmlI CAC'GTG
Cuts at: 1501 1501
Size: 5072
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 4211 4243 244
Size: 32 3935 32 1073
Fragments arranged by size:
3935 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 444 628 1817 444
Size: 184 1189 3699
Fragments arranged by size:
3699 1189 184
PsiI TTA'TAA
Cuts at: 614 1600 614
Size: 986 4086
PspOMI G'GGCC_C
Cuts at: 1020 1020
Size: 5072
PspXI vC'TCGA_Gb
Cuts at: 889 889
Size: 5072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1651 1683 1651
Size: 32 5040
PstI C_TGCA'G
Cuts at: 858 2119 2804 858
Size: 1261 685 3126
Fragments arranged by size:
3126 1261 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5072
PvuII CAG'CTG
Cuts at: 381 2497 2857 381
Size: 2116 360 2596
Fragments arranged by size:
2596 2116 360
RsrII CG'GwC_CG
Cuts at: 3267 3267
Size: 5072
SacI G_AGCT'C
Cuts at: 377 1750 1918 2002 2086 377
Size: 1373 168 84 84 3363
Fragments arranged by size:
3363 1373 168 84 84
SalI G'TCGA_C
Cuts at: 2109 2109
Size: 5072
SapI GCTCTTCn'nnn_
Cuts at: 1513 1755 3095 3305 1513
Size: 242 1340 210 3280
Fragments arranged by size:
3280 1340 242 210
SbfI CC_TGCA'GG
Cuts at: 858 2119 858
Size: 1261 3811
ScaI AGT'ACT
Cuts at: 848 1581 4589 848
Size: 733 3008 1331
Fragments arranged by size:
3008 1331 733
SexAI A'CCwGG_T
Cuts at: 254 1073 254
Size: 819 4253
SfcI C'TryA_G
Cuts at: 33 246 676 854 2115 2800 3793 3984 33
Size: 213 430 178 1261 685 993 191 1121
Fragments arranged by size:
1261 1121 993 685 430 213 191 178
SfoI GGC'GCC
Cuts at: 2504 2752 2504
Size: 248 4824
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5072
SmaI CCC'GGG
Cuts at: 634 2094 634
Size: 1460 3612
SmlI C'TyrA_G
Cuts at: 347 889 1179 2227 3142 3634 3896 347
Size: 542 290 1048 915 492 262 1523
Fragments arranged by size:
1523 1048 915 542 492 290 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5072
SphI G_CATG'C
Cuts at: 1970 2125 2625 1970
Size: 155 500 4417
Fragments arranged by size:
4417 500 155
SspI AAT'ATT
Cuts at: 1652 1652
Size: 5072
StuI AGG'CCT
Cuts at: 1987 1987
Size: 5072
StyI C'CwwG_G
Cuts at: 107 695 735 1541 2206 2560 107
Size: 588 40 806 665 354 2619
Fragments arranged by size:
2619 806 665 588 354 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 756 797 1051 2008 2655 2788 3247 3456 756
Size: 41 254 957 647 133 459 209 2372
Fragments arranged by size:
2372 957 647 459 254 209 133 41
TatI w'GTAC_w
Cuts at: 221 846 1167 1579 4587 221
Size: 625 321 412 3008 706
Fragments arranged by size:
3008 706 625 412 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4589 4621 4589
Size: 32 5040
Tth111I GACn'n_nGTC
Cuts at: 2869 2869
Size: 5072
XbaI T'CTAG_A
Cuts at: 2103 2103
Size: 5072
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 288 466 1330 1396 2567 288
Size: 178 864 66 1171 2793
Fragments arranged by size:
2793 1171 864 178 66
XhoI C'TCGA_G
Cuts at: 889 889
Size: 5072
XmaI C'CCGG_G
Cuts at: 632 2092 632
Size: 1460 3612
XmnI GAAnn'nnTTC
Cuts at: 848 1567 848
Size: 719 4353
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI BaeI
BbsI BbvCI BglI BmgBI BmtI BsaXI BsiWI BsmBI
BstXI BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NheI NotI NruI PacI PasI PshAI
SacII SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI