Restriction map for
pF1KB6174
(Circular) (Six-base) MAPSORT of: pF1KB6174.seq Check: 3264 from: 1 to: 5003
pF1KB6174 5003 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 694 1128 1501 1575 694
Size: 434 373 74 4122
Fragments arranged by size:
4122 434 373 74
AccI GT'mk_AC
Cuts at: 1145 1772 2041 1145
Size: 627 269 4107
Fragments arranged by size:
4107 627 269
Acc65I G'GTAC_C
Cuts at: 2019 2019
Size: 5003
AclI AA'CG_TT
Cuts at: 562 4843 562
Size: 4281 722
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3260 4007 4275 2828
Size: 432 747 268 3556
Fragments arranged by size:
3556 747 432 268
AflIII A'CryG_T
Cuts at: 2355 3459 4436 2355
Size: 1104 977 2922
Fragments arranged by size:
2922 1104 977
AgeI A'CCGG_T
Cuts at: 3391 3391
Size: 5003
AleI CACnn'nnGTG
Cuts at: 1894 1894
Size: 5003
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1597 1597
Size: 5003
AlwNI CAG_nnn'CTG
Cuts at: 1047 1349 1360 2308 3875 1047
Size: 302 11 948 1567 2175
Fragments arranged by size:
2175 1567 948 302 11
ApaI G_GGCC'C
Cuts at: 145 271 1597 145
Size: 126 1326 3551
Fragments arranged by size:
3551 1326 126
ApaLI G'TGCA_C
Cuts at: 3773 3773
Size: 5003
ApoI r'AATT_y
Cuts at: 1264 2007 4356 4612 1264
Size: 743 2349 256 1655
Fragments arranged by size:
2349 1655 743 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5003
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
AvaI C'yCGr_G
Cuts at: 441 2023 441
Size: 1582 3421
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1931 1964 1931
Size: 33 4970
BamHI G'GATC_C
Cuts at: 233 2028 233
Size: 1795 3208
BanI G'GyrC_C
Cuts at: 538 863 899 1028 1304 1424 1587 1746 2019
Size: 325 36 129 276 120 163 159 273
Cuts at: 2019 2433 2681 2716 538
Size: 414 248 35 2825
Fragments arranged by size:
2825 414 325 276 273 248 163 159
129 120 36 35
BanII G_rGCy'C
Cuts at: 145 220 271 1041 1082 1104 1597 2017 3047
Size: 75 51 770 41 22 493 420 1030
Cuts at: 3047 145
Size: 2101
Fragments arranged by size:
2101 1030 770 493 420 75 51 41
22
BbeI G_GCGC'C
Cuts at: 867 1032 1308 2437 2685 867
Size: 165 276 1129 248 3185
Fragments arranged by size:
3185 1129 276 248 165
BbsI GAAGACnn'nnnn_
Cuts at: 671 671
Size: 5003
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 5003
BciVI GTATCCnnnnn_n'
Cuts at: 1686 2901 3662 5003 1686
Size: 1215 761 1341 1686
Fragments arranged by size:
1686 1341 1215 761
BclI T'GATC_A
Cuts at: 1898 2523 1898
Size: 625 4378
BglI GCCn_nnn'nGGC
Cuts at: 331 378 331
Size: 47 4956
BglII A'GATC_T
Cuts at: 2518 4555 2518
Size: 2037 2966
BlpI GC'TnA_GC
Cuts at: 2115 2115
Size: 5003
Bme1580I G_kGCm'C
Cuts at: 145 271 1597 2628 2721 3777 145
Size: 126 1326 1031 93 1056 1371
Fragments arranged by size:
1371 1326 1056 1031 126 93
BmrI ACTGGGnnnn_n'
Cuts at: 1337 1629 2215 2296 2631 4775 1337
Size: 292 586 81 335 2144 1565
Fragments arranged by size:
2144 1565 586 335 292 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2003
Size: 5003
Bpu10I CC'TnA_GC
Cuts at: 113 113
Size: 5003
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2179 3058 3550 3848 2179
Size: 879 492 298 3334
Fragments arranged by size:
3334 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 445 862 4707 445
Size: 417 3845 741
Fragments arranged by size:
3845 741 417
BsaAI yAC'GTr
Cuts at: 2356 2356
Size: 5003
BsaBI GATnn'nnATC
Cuts at: 6 2517 4554 6
Size: 2511 2037 455
Fragments arranged by size:
2511 2037 455
BsaHI Gr'CG_yC
Cuts at: 864 1029 1305 2434 2682 3384 4880 864
Size: 165 276 1129 248 702 1496 987
Fragments arranged by size:
1496 1129 987 702 276 248 165
BsaWI w'CCGG_w
Cuts at: 755 2192 2713 3391 3665 3812 4223 755
Size: 1437 521 678 274 147 411 1535
Fragments arranged by size:
1535 1437 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 737 767 1476 1506 1621 1651 737
Size: 30 709 30 115 30 4089
Fragments arranged by size:
4089 709 115 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1831 1843 1831
Size: 12 4991
BseYI C'CCAG_C
Cuts at: 477 1040 1408 2428 3186 3763 4259 477
Size: 563 368 1020 758 577 496 1221
Fragments arranged by size:
1221 1020 758 577 563 496 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 665 1226 1603 665
Size: 561 377 4065
Fragments arranged by size:
4065 561 377
BsiEI CG_ry'CG
Cuts at: 104 2545 2591 3799 104
Size: 2441 46 1208 1308
Fragments arranged by size:
2441 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 585 1642 2017 2795 2985 3777 585
Size: 1057 375 778 190 792 1811
Fragments arranged by size:
1811 1057 792 778 375 190
BsmBI CGTCTCn'nnnn_
Cuts at: 738 738
Size: 5003
Bsp1286I G_dGCh'C
Cuts at: 145 220 271 585 1041 1082 1104 1597 1642
Size: 75 51 314 456 41 22 493 45
Cuts at: 1642 2017 2628 2721 2795 2985 3047 3777 145
Size: 375 611 93 74 190 62 730 1371
Fragments arranged by size:
1371 730 611 493 456 375 314 190
93 75 74 62 51 45 41 22
BspEI T'CCGG_A
Cuts at: 755 4223 755
Size: 3468 1535
BspHI T'CATG_A
Cuts at: 4179 4179
Size: 5003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 694 1128 1501 1575 1986 2053 2267 2569 2950
Size: 434 373 74 411 67 214 302 381
Cuts at: 2950 694
Size: 2747
Fragments arranged by size:
2747 434 411 381 373 302 214 74
67
BsrBI CCG'CTC
Cuts at: 50 3295 3349 4833 4998 50
Size: 3245 54 1484 165 55
Fragments arranged by size:
3245 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 822 2915 822
Size: 2093 2910
BsrFI r'CCGG_y
Cuts at: 1178 3001 3182 3391 1178
Size: 1823 181 209 2790
Fragments arranged by size:
2790 1823 209 181
BssSI C'ACGA_G
Cuts at: 3274 3632 3274
Size: 358 4645
BstAPI GCAn_nnn'nTGC
Cuts at: 314 2380 314
Size: 2066 2937
BstBI TT'CG_AA
Cuts at: 3364 3364
Size: 5003
BstXI CCAn_nnnn'nTGG
Cuts at: 1104 1642 1104
Size: 538 4465
BstYI r'GATC_y
Cuts at: 1 233 2028 2495 2518 2853 3099 4111 4197
Size: 232 1795 467 23 335 246 1012 86
Cuts at: 4197 4209 4555 1
Size: 12 346 449
Fragments arranged by size:
1795 1012 467 449 346 335 246 232
86 23 12
BtgI C'CryG_G
Cuts at: 107 341 107
Size: 234 4769
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2953 3134 3297 2953
Size: 181 163 4659
Fragments arranged by size:
4659 181 163
BtsI GCAGTG_nn'
Cuts at: 1045 2246 1045
Size: 1201 3802
DraI TTT'AAA
Cuts at: 2002 2002
Size: 5003
DrdI GACnn_nn'nnGTC
Cuts at: 2709 3567 2709
Size: 858 4145
EaeI y'GGCC_r
Cuts at: 819 1233 2588 2762 3153 3180 4525 819
Size: 414 1355 174 391 27 1345 1297
Fragments arranged by size:
1355 1345 1297 414 391 174 27
EagI C'GGCC_G
Cuts at: 2588 2588
Size: 5003
EarI CTCTTCn'nnn_
Cuts at: 295 1865 3026 3236 295
Size: 1570 1161 210 2062
Fragments arranged by size:
2062 1570 1161 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1236 1705 3211 3521 3667 4813 1236
Size: 469 1506 310 146 1146 1426
Fragments arranged by size:
1506 1426 1146 469 310 146
EcoICRI GAG'CTC
Cuts at: 2015 2015
Size: 5003
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2003 2828 3260 4007 4275 2003
Size: 825 432 747 268 2731
Fragments arranged by size:
2731 825 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1624 1624
Size: 5003
EcoO109I rG'GnC_Cy
Cuts at: 142 268 438 674 1199 1594 2142 142
Size: 126 170 236 525 395 548 3003
Fragments arranged by size:
3003 548 525 395 236 170 126
EcoRI G'AATT_C
Cuts at: 2007 2007
Size: 5003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1470 3022 1470
Size: 1552 3451
FspI TGC'GCA
Cuts at: 2784 2784
Size: 5003
HaeII r_GCGC'y
Cuts at: 159 867 1032 1290 1308 2437 2685 3707 4686
Size: 708 165 258 18 1129 248 1022 979
Cuts at: 4686 159
Size: 476
Fragments arranged by size:
1129 1022 979 708 476 258 248 165
18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 700 732 737 769 1834 1866 2845
Size: 32 450 32 5 32 1065 32 979
Cuts at: 2845 2875 2877 2907 218
Size: 30 2 30 2314
Fragments arranged by size:
2314 1065 979 450 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 2042 2042
Size: 5003
KasI G'GCGC_C
Cuts at: 863 1028 1304 2433 2681 863
Size: 165 276 1129 248 3185
Fragments arranged by size:
3185 1129 276 248 165
KpnI G_GTAC'C
Cuts at: 2023 2023
Size: 5003
MluI A'CGCG_T
Cuts at: 4436 4436
Size: 5003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 387 1519 3674 3858 387
Size: 1132 2155 184 1532
Fragments arranged by size:
2155 1532 1132 184
MscI TGG'CCA
Cuts at: 821 2764 821
Size: 1943 3060
MslI CAynn'nnrTG
Cuts at: 1894 2992 3119 1894
Size: 1098 127 3778
Fragments arranged by size:
3778 1098 127
MspA1I CmG'CkG
Cuts at: 552 699 1238 1723 2114 2428 2788 3801 4046
Size: 147 539 485 391 314 360 1013 245
Cuts at: 4046 552
Size: 1509
Fragments arranged by size:
1509 1013 539 485 391 360 314 245
147
NaeI GCC'GGC
Cuts at: 1180 3184 1180
Size: 2004 2999
NarI GG'CG_CC
Cuts at: 864 1029 1305 2434 2682 864
Size: 165 276 1129 248 3185
Fragments arranged by size:
3185 1129 276 248 165
NcoI C'CATG_G
Cuts at: 107 107
Size: 5003
NgoMIV G'CCGG_C
Cuts at: 1178 3182 1178
Size: 2004 2999
NspI r_CATG'y
Cuts at: 985 2056 2556 3463 985
Size: 1071 500 907 2525
Fragments arranged by size:
2525 1071 907 500
PasI CC'CwG_GG
Cuts at: 138 138
Size: 5003
PciI A'CATG_T
Cuts at: 3459 3459
Size: 5003
PflMI CCAn_nnn'nTGG
Cuts at: 342 711 1349 342
Size: 369 638 3996
Fragments arranged by size:
3996 638 369
PmeI GTTT'AAAC
Cuts at: 2002 2002
Size: 5003
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 4142
Size: 32 4971
PspOMI G'GGCC_C
Cuts at: 141 267 1593 141
Size: 126 1326 3551
Fragments arranged by size:
3551 1326 126
PstI C_TGCA'G
Cuts at: 698 1319 2050 2735 698
Size: 621 731 685 2966
Fragments arranged by size:
2966 731 685 621
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5003
PvuII CAG'CTG
Cuts at: 699 1723 2428 2788 699
Size: 1024 705 360 2914
Fragments arranged by size:
2914 1024 705 360
RsrII CG'GwC_CG
Cuts at: 3198 3198
Size: 5003
SacI G_AGCT'C
Cuts at: 2017 2017
Size: 5003
SalI G'TCGA_C
Cuts at: 2040 2040
Size: 5003
SapI GCTCTTCn'nnn_
Cuts at: 3026 3236 3026
Size: 210 4793
SbfI CC_TGCA'GG
Cuts at: 2050 2050
Size: 5003
ScaI AGT'ACT
Cuts at: 4520 4520
Size: 5003
SfcI C'TryA_G
Cuts at: 33 694 1159 1315 2046 2731 3724 3915 33
Size: 661 465 156 731 685 993 191 1121
Fragments arranged by size:
1121 993 731 685 661 465 191 156
SfoI GGC'GCC
Cuts at: 865 1030 1306 2435 2683 865
Size: 165 276 1129 248 3185
Fragments arranged by size:
3185 1129 276 248 165
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5003
SmaI CCC'GGG
Cuts at: 2025 2025
Size: 5003
SmlI C'TyrA_G
Cuts at: 2158 3073 3565 3827 2158
Size: 915 492 262 3334
Fragments arranged by size:
3334 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5003
SphI G_CATG'C
Cuts at: 2056 2556 2056
Size: 500 4503
StuI AGG'CCT
Cuts at: 1781 1781
Size: 5003
StyI C'CwwG_G
Cuts at: 107 329 455 1454 1957 2137 2491 107
Size: 222 126 999 503 180 354 2619
Fragments arranged by size:
2619 999 503 354 222 180 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 488 556 1909 2586 2719 3178 3387 488
Size: 68 1353 677 133 459 209 2104
Fragments arranged by size:
2104 1353 677 459 209 133 68
TatI w'GTAC_w
Cuts at: 4518 4518
Size: 5003
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1215 1247 1621 1653 4520 4552 1215
Size: 32 374 32 2867 32 1666
Fragments arranged by size:
2867 1666 374 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2800 2800
Size: 5003
XbaI T'CTAG_A
Cuts at: 2034 2034
Size: 5003
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 814 2498 814
Size: 1684 3319
XmaI C'CCGG_G
Cuts at: 2023 2023
Size: 5003
XmnI GAAnn'nnTTC
Cuts at: 167 167
Size: 5003
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BcgI
BfrBI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI