Restriction map for
pF1KB6176
(Circular) (Six-base) MAPSORT of: pF1KB6176.seq Check: 2306 from: 1 to: 4931
pF1KB6176 4931 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1164 1295 1164
Size: 131 4800
AatII G_ACGT'C
Cuts at: 1583 1583
Size: 4931
AccI GT'mk_AC
Cuts at: 176 230 290 1969 176
Size: 54 60 1679 3138
Fragments arranged by size:
3138 1679 60 54
Acc65I G'GTAC_C
Cuts at: 992 1020 1947 992
Size: 28 927 3976
Fragments arranged by size:
3976 927 28
AclI AA'CG_TT
Cuts at: 4771 4771
Size: 4931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 411 1066 2756 3188 3935 4203 274
Size: 137 655 1690 432 747 268 1002
Fragments arranged by size:
1690 1002 747 655 432 268 137
AflII C'TTAA_G
Cuts at: 454 454
Size: 4931
AflIII A'CryG_T
Cuts at: 1194 2283 3387 4364 1194
Size: 1089 1104 977 1761
Fragments arranged by size:
1761 1104 1089 977
AgeI A'CCGG_T
Cuts at: 3319 3319
Size: 4931
AleI CACnn'nnGTG
Cuts at: 202 1257 202
Size: 1055 3876
AlwNI CAG_nnn'CTG
Cuts at: 1044 1513 1718 1797 2236 3803 1044
Size: 469 205 79 439 1567 2172
Fragments arranged by size:
2172 1567 469 439 205 79
ApaI G_GGCC'C
Cuts at: 127 1488 127
Size: 1361 3570
ApaLI G'TGCA_C
Cuts at: 3701 3701
Size: 4931
ApoI r'AATT_y
Cuts at: 1935 4284 4540 1935
Size: 2349 256 2326
Fragments arranged by size:
2349 2326 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4931
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
AvaI C'yCGr_G
Cuts at: 152 869 900 1951 152
Size: 717 31 1051 3132
Fragments arranged by size:
3132 1051 717 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 534 567 534
Size: 33 4898
BamHI G'GATC_C
Cuts at: 1956 1956
Size: 4931
BanI G'GyrC_C
Cuts at: 163 992 1020 1072 1736 1947 2361 2609 2644
Size: 829 28 52 664 211 414 248 35
Cuts at: 2644 163
Size: 2450
Fragments arranged by size:
2450 829 664 414 248 211 52 35
28
BanII G_rGCy'C
Cuts at: 127 365 880 1066 1270 1430 1488 1945 2975
Size: 238 515 186 204 160 58 457 1030
Cuts at: 2975 127
Size: 2083
Fragments arranged by size:
2083 1030 515 457 238 204 186 160
58
BbeI G_GCGC'C
Cuts at: 1740 2365 2613 1740
Size: 625 248 4058
Fragments arranged by size:
4058 625 248
BbsI GAAGACnn'nnnn_
Cuts at: 424 1192 1474 424
Size: 768 282 3881
Fragments arranged by size:
3881 768 282
BbvCI CC'TCA_GC
Cuts at: 1532 1699 1532
Size: 167 4764
BciVI GTATCCnnnnn_n'
Cuts at: 2829 3590 4931 2829
Size: 761 1341 2829
Fragments arranged by size:
2829 1341 761
BclI T'GATC_A
Cuts at: 2451 2451
Size: 4931
BglI GCCn_nnn'nGGC
Cuts at: 139 1643 139
Size: 1504 3427
BglII A'GATC_T
Cuts at: 1392 2446 4483 1392
Size: 1054 2037 1840
Fragments arranged by size:
2037 1840 1054
BlpI GC'TnA_GC
Cuts at: 1142 2043 1142
Size: 901 4030
Bme1580I G_kGCm'C
Cuts at: 127 166 1488 2556 2649 3705 127
Size: 39 1322 1068 93 1056 1353
Fragments arranged by size:
1353 1322 1068 1056 93 39
BmgBI CAC'GTC
Cuts at: 746 1332 746
Size: 586 4345
BmrI ACTGGGnnnn_n'
Cuts at: 1022 1023 2143 2224 2559 4703 1022
Size: 1 1120 81 335 2144 1250
Fragments arranged by size:
2144 1250 1120 335 81 1
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 636 1521 3298 636
Size: 885 1777 2269
Fragments arranged by size:
2269 1777 885
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 585 1254 1695 585
Size: 669 441 3821
Fragments arranged by size:
3821 669 441
Bpu10I CC'TnA_GC
Cuts at: 1180 1532 1699 1180
Size: 352 167 4412
Fragments arranged by size:
4412 352 167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1381 1741 2107 2986 3478 3776 1105
Size: 276 360 366 879 492 298 2260
Fragments arranged by size:
2260 879 492 366 360 298 276
BsaI GGTCTCn'nnnn_
Cuts at: 4635 4635
Size: 4931
BsaAI yAC'GTr
Cuts at: 2284 2284
Size: 4931
BsaBI GATnn'nnATC
Cuts at: 6 2445 4482 6
Size: 2439 2037 455
Fragments arranged by size:
2439 2037 455
BsaHI Gr'CG_yC
Cuts at: 1580 1737 2362 2610 3312 4808 1580
Size: 157 625 248 702 1496 1703
Fragments arranged by size:
1703 1496 702 625 248 157
BsaWI w'CCGG_w
Cuts at: 592 2120 2641 3319 3593 3740 4151 592
Size: 1528 521 678 274 147 411 1372
Fragments arranged by size:
1528 1372 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 735 765 1811 1841 735
Size: 30 1046 30 3825
Fragments arranged by size:
3825 1046 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 863 1786 373
Size: 490 923 3518
Fragments arranged by size:
3518 923 490
BseYI C'CCAG_C
Cuts at: 661 674 711 880 885 1130 1487 2356 3114
Size: 13 37 169 5 245 357 869 758
Cuts at: 3114 3691 4187 661
Size: 577 496 1405
Fragments arranged by size:
1405 869 758 577 496 357 245 169
37 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1057
Size: 4931
BsiEI CG_ry'CG
Cuts at: 104 1234 2473 2519 3727 104
Size: 1130 1239 46 1208 1308
Fragments arranged by size:
1308 1239 1208 1130 46
BsiHKAI G_wGCw'C
Cuts at: 365 695 1121 1181 1604 1945 2723 2913 3705
Size: 330 426 60 423 341 778 190 792
Cuts at: 3705 365
Size: 1591
Fragments arranged by size:
1591 792 778 426 423 341 330 190
60
Bsp1286I G_dGCh'C
Cuts at: 127 166 365 695 880 1066 1121 1181 1270
Size: 39 199 330 185 186 55 60 89
Cuts at: 1270 1430 1488 1604 1945 2556 2649 2723 2913
Size: 160 58 116 341 611 93 74 190
Cuts at: 2913 2975 3705 127
Size: 62 730 1353
Fragments arranged by size:
1353 730 611 341 330 199 190 186
185 160 116 93 89 74 62 60
58 55 39
BspEI T'CCGG_A
Cuts at: 592 4151 592
Size: 3559 1372
BspHI T'CATG_A
Cuts at: 1223 4107 1223
Size: 2884 2047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 188 1164 1295 1981 2195 2497 2878 188
Size: 976 131 686 214 302 381 2241
Fragments arranged by size:
2241 976 686 381 302 214 131
BsrBI CCG'CTC
Cuts at: 50 740 948 1481 1776 1842 3223 3277 4761
Size: 690 208 533 295 66 1381 54 1484
Cuts at: 4761 4926 50
Size: 165 55
Fragments arranged by size:
1484 1381 690 533 295 208 165 66
55 54
BsrDI GCAATG_nn'
Cuts at: 338 2843 338
Size: 2505 2426
BsrFI r'CCGG_y
Cuts at: 838 2929 3110 3319 838
Size: 2091 181 209 2450
Fragments arranged by size:
2450 2091 209 181
BssSI C'ACGA_G
Cuts at: 3202 3560 3202
Size: 358 4573
BstAPI GCAn_nnn'nTGC
Cuts at: 2308 2308
Size: 4931
BstBI TT'CG_AA
Cuts at: 3292 3292
Size: 4931
BstXI CCAn_nnnn'nTGG
Cuts at: 490 811 490
Size: 321 4610
BstYI r'GATC_y
Cuts at: 1 1392 1510 1793 1956 2423 2446 2781 3027
Size: 1391 118 283 163 467 23 335 246
Cuts at: 3027 4039 4125 4137 4483 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1391 1012 467 449 346 335 283 246
163 118 86 23 12
BstZ17I GTA'TAC
Cuts at: 177 177
Size: 4931
Bsu36I CC'TnA_GG
Cuts at: 1506 1506
Size: 4931
BtgI C'CryG_G
Cuts at: 107 118 468 776 785 1677 107
Size: 11 350 308 9 892 3361
Fragments arranged by size:
3361 892 350 308 11 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1115 2881 3062 3225 1115
Size: 1766 181 163 2821
Fragments arranged by size:
2821 1766 181 163
BtsI GCAGTG_nn'
Cuts at: 164 320 1624 2174 164
Size: 156 1304 550 2921
Fragments arranged by size:
2921 1304 550 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 493 528 493
Size: 35 4896
DraI TTT'AAA
Cuts at: 1930 1930
Size: 4931
DraIII CAC_nnn'GTG
Cuts at: 1030 1256 1030
Size: 226 4705
DrdI GACnn_nn'nnGTC
Cuts at: 2637 3495 2637
Size: 858 4073
EaeI y'GGCC_r
Cuts at: 813 959 1106 1231 1295 1457 1799 2516 2690
Size: 146 147 125 64 162 342 717 174
Cuts at: 2690 3081 3108 4453 813
Size: 391 27 1345 1291
Fragments arranged by size:
1345 1291 717 391 342 174 162 147
146 125 64 27
EagI C'GGCC_G
Cuts at: 1231 2516 1231
Size: 1285 3646
EarI CTCTTCn'nnn_
Cuts at: 1042 1789 2954 3164 1042
Size: 747 1165 210 2809
Fragments arranged by size:
2809 1165 747 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3139 3449 3595 4741 3139
Size: 310 146 1146 3329
Fragments arranged by size:
3329 1146 310 146
EcoICRI GAG'CTC
Cuts at: 363 1943 363
Size: 1580 3351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 274 411 585 1066 1254 1695 2756 3188 3935
Size: 137 174 481 188 441 1061 432 747
Cuts at: 3935 4203 274
Size: 268 1002
Fragments arranged by size:
1061 1002 747 481 441 432 268 188
174 137
EcoNI CCTnn'n_nnAGG
Cuts at: 903 1420 903
Size: 517 4414
EcoO109I rG'GnC_Cy
Cuts at: 123 124 249 375 525 658 1484 1551 2070
Size: 1 125 126 150 133 826 67 519
Cuts at: 2070 123
Size: 2984
Fragments arranged by size:
2984 826 519 150 133 126 125 67
1
EcoRI G'AATT_C
Cuts at: 1935 1935
Size: 4931
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 441 2950 441
Size: 2509 2422
FspI TGC'GCA
Cuts at: 2712 2712
Size: 4931
HaeII r_GCGC'y
Cuts at: 1740 2365 2613 3635 4614 1740
Size: 625 248 1022 979 2057
Fragments arranged by size:
2057 1022 979 625 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 937 969 1229 1261 1384 1416 2773 2803 2805
Size: 32 260 32 123 32 1357 30 2
Cuts at: 2805 2835 937
Size: 30 3033
Fragments arranged by size:
3033 1357 260 123 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 543 1200 1970 543
Size: 657 770 3504
Fragments arranged by size:
3504 770 657
KasI G'GCGC_C
Cuts at: 1736 2361 2609 1736
Size: 625 248 4058
Fragments arranged by size:
4058 625 248
KpnI G_GTAC'C
Cuts at: 996 1024 1951 996
Size: 28 927 3976
Fragments arranged by size:
3976 927 28
MfeI C'AATT_G
Cuts at: 1805 1805
Size: 4931
MluI A'CGCG_T
Cuts at: 4364 4364
Size: 4931
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 818 3602 3786 818
Size: 2784 184 1963
Fragments arranged by size:
2784 1963 184
MscI TGG'CCA
Cuts at: 815 1459 1801 2692 815
Size: 644 342 891 3054
Fragments arranged by size:
3054 891 644 342
MslI CAynn'nnrTG
Cuts at: 202 1100 1257 1289 1330 2920 3047 202
Size: 898 157 32 41 1590 127 2086
Fragments arranged by size:
2086 1590 898 157 127 41 32
MspA1I CmG'CkG
Cuts at: 393 1041 1141 1531 1767 2042 2356 2716 3729
Size: 648 100 390 236 275 314 360 1013
Cuts at: 3729 3974 393
Size: 245 1350
Fragments arranged by size:
1350 1013 648 390 360 314 275 245
236 100
NaeI GCC'GGC
Cuts at: 3112 3112
Size: 4931
NarI GG'CG_CC
Cuts at: 1737 2362 2610 1737
Size: 625 248 4058
Fragments arranged by size:
4058 625 248
NcoI C'CATG_G
Cuts at: 107 468 785 107
Size: 361 317 4253
Fragments arranged by size:
4253 361 317
NdeI CA'TA_TG
Cuts at: 764 764
Size: 4931
NgoMIV G'CCGG_C
Cuts at: 3110 3110
Size: 4931
NspI r_CATG'y
Cuts at: 1198 1294 1984 2484 3391 1198
Size: 96 690 500 907 2738
Fragments arranged by size:
2738 907 690 500 96
PasI CC'CwG_GG
Cuts at: 654 1350 654
Size: 696 4235
PciI A'CATG_T
Cuts at: 1194 3387 1194
Size: 2193 2738
PflMI CCAn_nnn'nTGG
Cuts at: 1356 1494 1797 1356
Size: 138 303 4490
Fragments arranged by size:
4490 303 138
PfoI T'CCnGG_A
Cuts at: 212 581 212
Size: 369 4562
PmeI GTTT'AAAC
Cuts at: 1930 1930
Size: 4931
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4070 4102 4070
Size: 32 4899
PpuMI rG'GwC_Cy
Cuts at: 375 525 658 1551 375
Size: 150 133 893 3755
Fragments arranged by size:
3755 893 150 133
PspOMI G'GGCC_C
Cuts at: 123 1484 123
Size: 1361 3570
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 976 1008 976
Size: 32 4899
PstI C_TGCA'G
Cuts at: 1978 2663 1978
Size: 685 4246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4931
PvuII CAG'CTG
Cuts at: 1041 1141 1531 2356 2716 1041
Size: 100 390 825 360 3256
Fragments arranged by size:
3256 825 390 360 100
RsrII CG'GwC_CG
Cuts at: 3126 3126
Size: 4931
SacI G_AGCT'C
Cuts at: 365 1945 365
Size: 1580 3351
SalI G'TCGA_C
Cuts at: 1968 1968
Size: 4931
SanDI GG'GwC_CC
Cuts at: 658 658
Size: 4931
SapI GCTCTTCn'nnn_
Cuts at: 2954 3164 2954
Size: 210 4721
SbfI CC_TGCA'GG
Cuts at: 1978 1978
Size: 4931
ScaI AGT'ACT
Cuts at: 4448 4448
Size: 4931
SfcI C'TryA_G
Cuts at: 33 727 1563 1974 2659 3652 3843 33
Size: 694 836 411 685 993 191 1121
Fragments arranged by size:
1121 993 836 694 685 411 191
SfoI GGC'GCC
Cuts at: 1738 2363 2611 1738
Size: 625 248 4058
Fragments arranged by size:
4058 625 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4931
SmaI CCC'GGG
Cuts at: 154 871 1953 154
Size: 717 1082 3132
Fragments arranged by size:
3132 1082 717
SmlI C'TyrA_G
Cuts at: 454 1120 1396 1756 2086 3001 3493 3755 454
Size: 666 276 360 330 915 492 262 1630
Fragments arranged by size:
1630 915 666 492 360 330 276 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4931
SphI G_CATG'C
Cuts at: 1984 2484 1984
Size: 500 4431
StyI C'CwwG_G
Cuts at: 107 468 521 785 956 1493 1724 2065 2419
Size: 361 53 264 171 537 231 341 354
Cuts at: 2419 107
Size: 2619
Fragments arranged by size:
2619 537 361 354 341 264 231 171
53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 1041 1888 2514 2647 3106 3315 688
Size: 353 847 626 133 459 209 2304
Fragments arranged by size:
2304 847 626 459 353 209 133
TatI w'GTAC_w
Cuts at: 4446 4446
Size: 4931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 735 767 4448 4480 735
Size: 32 3681 32 1186
Fragments arranged by size:
3681 1186 32 32
Tth111I GACn'n_nGTC
Cuts at: 1315 2728 1315
Size: 1413 3518
XbaI T'CTAG_A
Cuts at: 240 1962 240
Size: 1722 3209
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 345 762 783 2426 345
Size: 417 21 1643 2850
Fragments arranged by size:
2850 1643 417 21
XmaI C'CCGG_G
Cuts at: 152 869 1951 152
Size: 717 1082 3132
Fragments arranged by size:
3132 1082 717
XmnI GAAnn'nnTTC
Cuts at: 421 421
Size: 4931
ZraI GAC'GTC
Cuts at: 1581 1581
Size: 4931
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AhdI AlfI AloI AscI AvrII BcgI BfrBI
BmtI BsiWI BsmI BsmBI BsrGI BssHII BstEII ClaI
EcoRV FseI FspAI HindIII HpaI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI SacII SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI