Restriction map for
pF1KB6179
(Circular) (Six-base) MAPSORT of: pF1KB6179.seq Check: 4396 from: 1 to: 4934
pF1KB6179 4934 bp
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 509 1972 509
Size: 1463 3471
Acc65I G'GTAC_C
Cuts at: 1950 1950
Size: 4934
AclI AA'CG_TT
Cuts at: 4774 4774
Size: 4934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1253 1738 1816 2759 3191 3938 4206 1180
Size: 73 485 78 943 432 747 268 1908
Fragments arranged by size:
1908 943 747 485 432 268 78 73
AflII C'TTAA_G
Cuts at: 877 877
Size: 4934
AflIII A'CryG_T
Cuts at: 1391 1847 2286 3390 4367 1391
Size: 456 439 1104 977 1958
Fragments arranged by size:
1958 1104 977 456 439
AgeI A'CCGG_T
Cuts at: 3322 3322
Size: 4934
AleI CACnn'nnGTG
Cuts at: 517 517
Size: 4934
AlwNI CAG_nnn'CTG
Cuts at: 871 1767 1893 2239 3806 871
Size: 896 126 346 1567 1999
Fragments arranged by size:
1999 1567 896 346 126
ApaI G_GGCC'C
Cuts at: 162 162
Size: 4934
ApaLI G'TGCA_C
Cuts at: 3704 3704
Size: 4934
ApoI r'AATT_y
Cuts at: 1938 4287 4543 1938
Size: 2349 256 2329
Fragments arranged by size:
2349 2329 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4934
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
AvaI C'yCGr_G
Cuts at: 299 337 800 1625 1954 299
Size: 38 463 825 329 3279
Fragments arranged by size:
3279 825 463 329 38
BamHI G'GATC_C
Cuts at: 1503 1959 1503
Size: 456 4478
BanI G'GyrC_C
Cuts at: 114 165 189 1950 2364 2612 2647 114
Size: 51 24 1761 414 248 35 2401
Fragments arranged by size:
2401 1761 414 248 51 35 24
BanII G_rGCy'C
Cuts at: 162 338 1424 1732 1948 2978 162
Size: 176 1086 308 216 1030 2118
Fragments arranged by size:
2118 1086 1030 308 216 176
BbeI G_GCGC'C
Cuts at: 118 169 2368 2616 118
Size: 51 2199 248 2436
Fragments arranged by size:
2436 2199 248 51
BbsI GAAGACnn'nnnn_
Cuts at: 619 1093 1726 619
Size: 474 633 3827
Fragments arranged by size:
3827 633 474
BciVI GTATCCnnnnn_n'
Cuts at: 542 735 2832 3593 4934 542
Size: 193 2097 761 1341 542
Fragments arranged by size:
2097 1341 761 542 193
BclI T'GATC_A
Cuts at: 200 2454 200
Size: 2254 2680
BglI GCCn_nnn'nGGC
Cuts at: 112 349 112
Size: 237 4697
BglII A'GATC_T
Cuts at: 2449 4486 2449
Size: 2037 2897
BlpI GC'TnA_GC
Cuts at: 1814 2046 1814
Size: 232 4702
Bme1580I G_kGCm'C
Cuts at: 162 1284 2559 2652 3708 162
Size: 1122 1275 93 1056 1388
Fragments arranged by size:
1388 1275 1122 1056 93
BmgBI CAC'GTC
Cuts at: 213 296 213
Size: 83 4851
BmrI ACTGGGnnnn_n'
Cuts at: 454 543 831 2146 2227 2562 4706 454
Size: 89 288 1315 81 335 2144 682
Fragments arranged by size:
2144 1315 682 335 288 89 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 4934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 1684
Size: 4934
Bpu10I CC'TnA_GC
Cuts at: 232 248 332 232
Size: 16 84 4834
Fragments arranged by size:
4834 84 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2110 2989 3481 3779 1822
Size: 288 879 492 298 2977
Fragments arranged by size:
2977 879 492 298 288
BsaI GGTCTCn'nnnn_
Cuts at: 1594 4638 1594
Size: 3044 1890
BsaAI yAC'GTr
Cuts at: 409 1850 2287 409
Size: 1441 437 3056
Fragments arranged by size:
3056 1441 437
BsaBI GATnn'nnATC
Cuts at: 6 1051 2448 4485 6
Size: 1045 1397 2037 455
Fragments arranged by size:
2037 1397 1045 455
BsaHI Gr'CG_yC
Cuts at: 115 166 2365 2613 3315 4811 115
Size: 51 2199 248 702 1496 238
Fragments arranged by size:
2199 1496 702 248 238 51
BsaWI w'CCGG_w
Cuts at: 2123 2644 3322 3596 3743 4154 2123
Size: 521 678 274 147 411 2903
Fragments arranged by size:
2903 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 171
Size: 4934
BseYI C'CCAG_C
Cuts at: 669 2359 3117 3694 4190 669
Size: 1690 758 577 496 1413
Fragments arranged by size:
1690 1413 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 1279 385
Size: 894 4040
BsiEI CG_ry'CG
Cuts at: 104 260 2476 2522 3730 104
Size: 156 2216 46 1208 1308
Fragments arranged by size:
2216 1308 1208 156 46
BsiHKAI G_wGCw'C
Cuts at: 338 1409 1948 2726 2916 3708 338
Size: 1071 539 778 190 792 1564
Fragments arranged by size:
1564 1071 792 778 539 190
Bsp1286I G_dGCh'C
Cuts at: 162 338 1284 1409 1424 1732 1948 2559 2652
Size: 176 946 125 15 308 216 611 93
Cuts at: 2652 2726 2916 2978 3708 162
Size: 74 190 62 730 1388
Fragments arranged by size:
1388 946 730 611 308 216 190 176
125 93 74 62 15
BspEI T'CCGG_A
Cuts at: 4154 4154
Size: 4934
BspHI T'CATG_A
Cuts at: 4110 4110
Size: 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 357 1539 1984 2198 2500 2881 357
Size: 1182 445 214 302 381 2410
Fragments arranged by size:
2410 1182 445 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3226 3280 4764 4929 50
Size: 3176 54 1484 165 55
Fragments arranged by size:
3176 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1008 2846 1008
Size: 1838 3096
BsrFI r'CCGG_y
Cuts at: 124 2932 3113 3322 124
Size: 2808 181 209 1736
Fragments arranged by size:
2808 1736 209 181
BssSI C'ACGA_G
Cuts at: 3205 3563 3205
Size: 358 4576
BstAPI GCAn_nnn'nTGC
Cuts at: 2311 2311
Size: 4934
BstBI TT'CG_AA
Cuts at: 3295 3295
Size: 4934
BstEII G'GTnAC_C
Cuts at: 1144 1896 1144
Size: 752 4182
BstYI r'GATC_y
Cuts at: 1 1503 1959 2426 2449 2784 3030 4042 4128
Size: 1502 456 467 23 335 246 1012 86
Cuts at: 4128 4140 4486 1
Size: 12 346 449
Fragments arranged by size:
1502 1012 467 456 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4934
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2884 3065 3228 2884
Size: 181 163 4590
Fragments arranged by size:
4590 181 163
BtsI GCAGTG_nn'
Cuts at: 1699 2177 1699
Size: 478 4456
DraI TTT'AAA
Cuts at: 750 1933 750
Size: 1183 3751
DrdI GACnn_nn'nnGTC
Cuts at: 1381 1603 2640 3498 1381
Size: 222 1037 858 2817
Fragments arranged by size:
2817 1037 858 222
EaeI y'GGCC_r
Cuts at: 257 1320 2519 2693 3084 3111 4456 257
Size: 1063 1199 174 391 27 1345 735
Fragments arranged by size:
1345 1199 1063 735 391 174 27
EagI C'GGCC_G
Cuts at: 257 2519 257
Size: 2262 2672
EarI CTCTTCn'nnn_
Cuts at: 383 1651 2957 3167 383
Size: 1268 1306 210 2150
Fragments arranged by size:
2150 1306 1268 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3142 3452 3598 4744 3142
Size: 310 146 1146 3332
Fragments arranged by size:
3332 1146 310 146
EcoICRI GAG'CTC
Cuts at: 336 1946 336
Size: 1610 3324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1180 1253 1684 1738 1816 2759 3191 3938 4206
Size: 73 431 54 78 943 432 747 268
Cuts at: 4206 1180
Size: 1908
Fragments arranged by size:
1908 943 747 432 431 268 78 73
54
EcoNI CCTnn'n_nnAGG
Cuts at: 247 247
Size: 4934
EcoO109I rG'GnC_Cy
Cuts at: 159 693 883 2073 159
Size: 534 190 1190 3020
Fragments arranged by size:
3020 1190 534 190
EcoRI G'AATT_C
Cuts at: 1938 1938
Size: 4934
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4934
FspI TGC'GCA
Cuts at: 2715 2715
Size: 4934
HaeII r_GCGC'y
Cuts at: 118 169 179 1821 1887 2368 2616 3638 4617
Size: 51 10 1642 66 481 248 1022 979
Cuts at: 4617 118
Size: 435
Fragments arranged by size:
1642 1022 979 481 435 248 66 51
10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1191 1223 2776 2806 2808 2838 1191
Size: 32 1553 30 2 30 3287
Fragments arranged by size:
3287 1553 32 30 30 2
HincII GTy'rAC
Cuts at: 1566 1973 1566
Size: 407 4527
HindIII A'AGCT_T
Cuts at: 634 634
Size: 4934
KasI G'GCGC_C
Cuts at: 114 165 2364 2612 114
Size: 51 2199 248 2436
Fragments arranged by size:
2436 2199 248 51
KpnI G_GTAC'C
Cuts at: 1954 1954
Size: 4934
MluI A'CGCG_T
Cuts at: 4367 4367
Size: 4934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 559 631 3605 3789 559
Size: 72 2974 184 1704
Fragments arranged by size:
2974 1704 184 72
MscI TGG'CCA
Cuts at: 2695 2695
Size: 4934
MslI CAynn'nnrTG
Cuts at: 517 628 793 2923 3050 517
Size: 111 165 2130 127 2401
Fragments arranged by size:
2401 2130 165 127 111
MspA1I CmG'CkG
Cuts at: 198 2045 2359 2719 3732 3977 198
Size: 1847 314 360 1013 245 1155
Fragments arranged by size:
1847 1155 1013 360 314 245
NaeI GCC'GGC
Cuts at: 126 3115 126
Size: 2989 1945
NarI GG'CG_CC
Cuts at: 115 166 2365 2613 115
Size: 51 2199 248 2436
Fragments arranged by size:
2436 2199 248 51
NcoI C'CATG_G
Cuts at: 107 107
Size: 4934
NgoMIV G'CCGG_C
Cuts at: 124 3113 124
Size: 2989 1945
NspI r_CATG'y
Cuts at: 1987 2487 3394 1987
Size: 500 907 3527
Fragments arranged by size:
3527 907 500
PciI A'CATG_T
Cuts at: 3390 3390
Size: 4934
PflMI CCAn_nnn'nTGG
Cuts at: 922 1893 1917 922
Size: 971 24 3939
Fragments arranged by size:
3939 971 24
PfoI T'CCnGG_A
Cuts at: 1916 1916
Size: 4934
PmeI GTTT'AAAC
Cuts at: 1933 1933
Size: 4934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4902
PpuMI rG'GwC_Cy
Cuts at: 693 693
Size: 4934
PshAI GACnn'nnGTC
Cuts at: 802 1561 802
Size: 759 4175
PspOMI G'GGCC_C
Cuts at: 158 158
Size: 4934
PspXI vC'TCGA_Gb
Cuts at: 337 337
Size: 4934
PstI C_TGCA'G
Cuts at: 1418 1709 1981 2666 1418
Size: 291 272 685 3686
Fragments arranged by size:
3686 685 291 272
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4934
PvuII CAG'CTG
Cuts at: 2359 2719 2359
Size: 360 4574
RsrII CG'GwC_CG
Cuts at: 3129 3129
Size: 4934
SacI G_AGCT'C
Cuts at: 338 1948 338
Size: 1610 3324
SalI G'TCGA_C
Cuts at: 1971 1971
Size: 4934
SapI GCTCTTCn'nnn_
Cuts at: 1651 2957 3167 1651
Size: 1306 210 3418
Fragments arranged by size:
3418 1306 210
SbfI CC_TGCA'GG
Cuts at: 1981 1981
Size: 4934
ScaI AGT'ACT
Cuts at: 4451 4451
Size: 4934
SfcI C'TryA_G
Cuts at: 33 732 1040 1414 1705 1977 2662 3655 3846
Size: 699 308 374 291 272 685 993 191
Cuts at: 3846 33
Size: 1121
Fragments arranged by size:
1121 993 699 685 374 308 291 272
191
SfoI GGC'GCC
Cuts at: 116 167 2366 2614 116
Size: 51 2199 248 2436
Fragments arranged by size:
2436 2199 248 51
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
SmaI CCC'GGG
Cuts at: 1627 1956 1627
Size: 329 4605
SmlI C'TyrA_G
Cuts at: 337 877 1837 2089 3004 3496 3758 337
Size: 540 960 252 915 492 262 1513
Fragments arranged by size:
1513 960 915 540 492 262 252
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4934
SphI G_CATG'C
Cuts at: 1987 2487 1987
Size: 500 4434
SspI AAT'ATT
Cuts at: 831 831
Size: 4934
StuI AGG'CCT
Cuts at: 1580 1681 1580
Size: 101 4833
StyI C'CwwG_G
Cuts at: 107 1499 2068 2422 107
Size: 1392 569 354 2619
Fragments arranged by size:
2619 1392 569 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 529 562 1132 2517 2650 3109 3318 529
Size: 33 570 1385 133 459 209 2145
Fragments arranged by size:
2145 1385 570 459 209 133 33
TatI w'GTAC_w
Cuts at: 753 4449 753
Size: 3696 1238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1488 1520 4451 4483 1488
Size: 32 2931 32 1939
Fragments arranged by size:
2931 1939 32 32
Tth111I GACn'n_nGTC
Cuts at: 2731 2731
Size: 4934
XbaI T'CTAG_A
Cuts at: 1965 1965
Size: 4934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1099 2429 1099
Size: 1330 3604
XhoI C'TCGA_G
Cuts at: 337 337
Size: 4934
XmaI C'CCGG_G
Cuts at: 1625 1954 1625
Size: 329 4605
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PsiI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XmnI ZraI